Reduced lecithin: cholesterol acyltransferase (LCAT) and Na+, K+, ATPase activity in diabetic patients.
Clin Biochem
; 30(2): 177-82, 1997 Mar.
Article
en En
| MEDLINE
| ID: mdl-9127701
ABSTRACT
OBJECTIVE:
To investigate the plasma concentrations of lecithincholesterol acyltransferase (LCAT EC 2.3.1.43) and erythrocyte membrane Na+, K+, ATPase and the correlation of these parameters in diabetes mellitus. DESIGN ANDMETHODS:
Na+, K+, ATPase was measured with spectrophotometric method and LCAT with radioactive method in 19 patients with insulin-dependent diabetes mellitus (IDDM), in 20 with non-insulin-dependent diabetes mellitus (NIDDM) and in 20 healthy volunteers as the control group.RESULTS:
Compared with the control group, plasma LCAT concentrations were found to be decreased in both of the patient groups (p < 0.01 for both). Erythrocyte membrane Na+, K+, ATPase activities were higher in the controls than both in the NIDDM and IDDM groups (p < 0.01 and p < 0.001, respectively). There were significant correlations between LCAT and Na+, K+, ATPase in IDDM (r = 0.82, p < 0.001) and in NIDDM (r = 0.74, p < 0.001). In order to investigate the effect of cholesterol (C) and lysophosphatidylcholine (LPC) on Na+, K+, ATPase activity, this enzyme's activity was determined in erythrocyte membranes obtained from diabetic subjects after in vitro incubation with increasing concentrations of LPC and C (2-10 microM). Enzymatic activity was significantly reduced by in vitro C at increasing concentrations but significantly increased by in vitro LPC at increasing concentrations.CONCLUSIONS:
From these data, it is to be concluded that the decrease in Na+, K+, ATPase activity in diabetes might be due to decreased LCAT concentrations and that may explain the development of atherosclerosis in diabetics.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ATPasa Intercambiadora de Sodio-Potasio
/
Diabetes Mellitus Tipo 1
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Diabetes Mellitus Tipo 2
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Fosfatidilcolina-Esterol O-Aciltransferasa
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Clin Biochem
Año:
1997
Tipo del documento:
Article