Peptide bank generated by large-scale preparation of circulating human peptides.
J Chromatogr A
; 776(1): 125-32, 1997 Jul 25.
Article
en En
| MEDLINE
| ID: mdl-9286086
ABSTRACT
Human hemofiltrate (HF) is a source for the purification of circulating regulatory peptides. HF is obtained in large quantities during treatment of patients suffering from chronic renal failure. We have developed a large-scale method for separating peptides from amounts up to 10,000 1 HF into 300 fractions in a standardized two-step procedure, employing cation-exchange separation, followed by reversed-phase chromatography. These fractions represent a peptide bank containing bioactive, desalted and lyophilized peptides of blood. Screening for and isolation of regulatory human peptides is simplified by using this peptide bank.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr A
Año:
1997
Tipo del documento:
Article
País de afiliación:
Alemania