Selective separation process of proteins based on the heat stress-induced translocation across phospholipid membranes.
J Chromatogr B Biomed Sci Appl
; 711(1-2): 111-6, 1998 Jun 26.
Article
en En
| MEDLINE
| ID: mdl-9699980
ABSTRACT
Heating of several protein solutions at 40-47 degrees C for 5-60 min in the presence of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes induced the translocation of beta-galactosidase (beta-gal), alpha-glucosidase (alpha-glu) and bovine carbonic anhydrase (CAB) from outer to inner aqueous phase across the liposome membrane. The translocated amounts of beta-gal at various temperatures were maximized under suitable heating conditions (45 degrees C, 30 min). Those of alpha-glu and CAB were maximized at 40-45 and 60 degrees C, respectively. Each maximum value could be correlated with the corresponding local hydrophobicity of each protein evaluated by the aqueous two-phase partitioning method. The possibility to apply these heat-induced translocation phenomena to the bioseparation of proteins was successfully demonstrated for the model mixture solution of beta-gal, alpha-glu and CAB.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Beta-Galactosidasa
/
Liposomas
Idioma:
En
Revista:
J Chromatogr B Biomed Sci Appl
Asunto de la revista:
QUIMICA CLINICA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón