DNA-binding properties of the overexpressed recombinant estrogen receptor alpha.
Biochem Mol Biol Int
; 45(4): 673-80, 1998 Jul.
Article
en En
| MEDLINE
| ID: mdl-9713689
ABSTRACT
Estrogen receptor alpha was overexpressed in COS-7 cells by transformation of an expression vector with the full length open reading frame of the receptor alpha. The nuclear estradiol-receptor complex and the soluble receptor from the COS-7 cells were cross-linked with an estrogen response element (ERE), which was substituted with 5-bromo-2'-deoxyuridine (BrdUVRE), as the receptor dimers by UV irradiation. In gel retardation analysis, the specific bindings of both nuclear and soluble receptors to ERE were decreased with increasing of KCl concentration compared with 0.1 M KCl. The ionic interactions of both receptors with ERE are thought to be similar.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Receptores de Estrógenos
/
Secuencias Reguladoras de Ácidos Nucleicos
Límite:
Animals
Idioma:
En
Revista:
Biochem Mol Biol Int
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón