Angiogenesis Induced by the Implantation of Autogenous Whole Bone Marrow Stem Cells in an Ischemic Animal Model
Journal of the Korean Society for Vascular Surgery
; : 113-117, 2005.
Article
en En
| WPRIM
| ID: wpr-22829
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE:
Bone marrow contains many kinds of primitive cells and endothelial progenitor cells that secrete several growth factors. We hypothesized that angiogenesis could be induced by autogenous whole bone marrow stem cell implantation in an animal ischemic limb.METHOD:
A chronic ischemic hind limb model was created by encircling the femoral artery with an ameroid constrictor (2 mm inner diameter) in a dog model. About 20 ml of autogenous whole bone marrow stem cells were aspirated from the femur and then injected into ischemic limb muscles. Contralateral limbs injected with 20 ml of normal saline as controls. To assess angiogenic effects, an angiogram and a histologic evaluation were performed at 8 weeks after bone marrow stem cell implantation.RESULT:
Imaging analysis of angiograms showed that newly developed capillaries were significantly more plentiful in treated limbs. Mean capillary density in the treated limb group was significantly greater than that in the control group (151+/-11.7 vs 81.5+/-7.2 cap/mm2, respectively, P<0.05), and the proportion of larger diameter (Fig. 6) newly developed capillaries was significantly higher in treated limbs than in control limbs.CONCLUSION:
These findings indicated that autogenous whole bone marrow stem cell implantation increases the efficiency of angiogenesis.Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Células Madre
/
Médula Ósea
/
Capilares
/
Modelos Animales
/
Péptidos y Proteínas de Señalización Intercelular
/
Extremidades
/
Arteria Femoral
/
Fémur
/
Músculos
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Journal of the Korean Society for Vascular Surgery
Año:
2005
Tipo del documento:
Article