Adhesion induces matrix metalloproteinase-9 gene expression in ovarian cancer cells / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 17-19, 2002.
Article
en Zh
| WPRIM
| ID: wpr-354084
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This work was conducted to investigate the expression of matrix metalloproteinase 9 (MMP 9) gene in cancer cells by fibronectin adhesion and the underlying mechanism of cell invasion.</p><p><b>METHODS</b>Following adhesion of ovarian cancer cells A2780 to fibronectin, mRNA expression of MMP cells were assayed by reverse transcription-polymerase chain reaction (RT-PCR). MMP9 promoter was cloned from genomic DNA of HT1080 cells with PCR. The MMP-9-pGL2 reporter gene vector was constructed and then transiently transfected into A2780 cells.</p><p><b>RESULTS</b>Adhesion induced the increase of cellular MMP9 mRNA content in A2780 cells, not affecting the expression of MMP2 or TIMP-1 gene. The stimulation was enhanced with the increase adhesion time. When the transfected cells were allowed to adhere and spread on FN-coated surface, the promoter activity of MMP9 gene was also enhanced dramatically.</p><p><b>CONCLUSION</b>Cell-ECM adhesion may stimulate the expression of MMP9 gene through stimulating the promoter activity, thereby enhancing cancer cell invasion and metastasis.</p>
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Neoplasias Ováricas
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Patología
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Fisiología
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Células Tumorales Cultivadas
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Expresión Génica
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Adhesión Celular
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Metaloproteinasa 9 de la Matriz
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Genética
Límite:
Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Año:
2002
Tipo del documento:
Article