Identification of specific peptides from Bubali Cornu by ultra-performance liquid chromatography-tandem mass spectrometry / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1279-1285, 2022.
Article
en Zh
| WPRIM
| ID: wpr-928053
Biblioteca responsable:
WPRO
ABSTRACT
This paper explored the specific peptides from Bubali Cornu by ultra-performance liquid chromatography-tandem mass spectrometry and based on mathematics set theory. Following the profile analysis of peptides from Bubali Cornu, Bovis Grunniens Cornu, Caprae Hircus Cornu, and Suis Cornu by nano LC-LTQ-Obitrap-MS after digestion with trypsin, the relationship of peptide composition among different samples was analyzed using the mathematics set theory. The ones that existed only in the Bubali Cornu set rather than in any other set were considered as the specific peptides of Bubali Cornu. The further bioinformatic analysis revealed four specific peptides from Bubali Cornu, whose specificity was verified by UPLC-QQQ-MS. The results showed that these four peptides could be used for distinguishing Bubali Cornu from Caprae Hircus Cornu and Suis Cornu. This study has provided a rapid and simple method for seeking the specific peptides in animal medicines, which can be utilized for quality evaluation of animal medicines, thus making them authenticable and traceable.
Palabras clave
Texto completo:
1
Banco de datos:
WPRIM
Asunto principal:
Péptidos
/
Cromatografía Liquida
/
Cornus
/
Espectrometría de Masas en Tándem
/
Cuernos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2022
Tipo del documento:
Article