Knowledge, Attitude, Practice, and Fear of COVID-19: an Online-Based Cross-cultural Study.

The study aimed to measure Knowledge, Attitude, and Practice (KAP) and fear level towards COVID-19 and explore its cross-cultural variances in knowledge by sociodemographic factors among the general population of 8 different countries over 5 continents. It was a cross-sectional online survey. This survey was conducted in April 2020 among 1296 participants using the "Google Form" platform. Considering the social distancing formula and pandemic situation, we collect data using popular social media networks. Univariate and bivariate analyses were used to explore the collected data on KAP, fear, and sociodemographic factors. Overall knowledge score was 9.7 ± 1.7 (out of 12), and gender differences (female vs male: 9.8 ± 1.6 vs 9.5 ± 1.9) were significant (p = 0.008) in the bivariate analysis. Knowledge score variances were found significant in some regions by gender, marital status, and education qualification. The highest and lowest mean knowledge scores were recorded in the Middle East (10.0 ± 1.7) and Europe (9.3 ± 2.0). Despite having a high fear score (22.5 ± 5.6 out of 35), 78.35% of respondents were positively and 81.7% in a good practice level. Fear score rankings: Middle East (1st; 23.8 ± 5.5), Europe (2nd; 23.2 ± 5.8), Africa (3rd; 22.7 ± 5.0), South Asia (4th; 22.1 ± 5.7), Oceania (5th; 21.9 ± 5.8), and North America (6th; 21.7 ± 5.5). Fear and knowledge were not correlated. KAP and fear variation exist among geographical regions. Gender, marital status, and education qualification are factors in knowledge variances for some regions. KAP and fear measures can help health education programs consider some sociodemographic factors and regions during an outbreak of highly contagious disease and uplift a positive attitude and good practice. Supplementary Information: The online version contains supplementary material available at 10.1007/s11469-021-00638-4.

Road traffic delays in commuting workplace and musculoskeletal health among sedentary workers: A cross-sectional study in Dhaka city.

OBJECTIVES: Despite previous research aimed at identifying factors linked to musculoskeletal health issues, there was no evidence about the relationship between road traffic delays (RTDs) and musculoskeletal health in sedentary employees. As a result, the aim of our research was to understand such a correlation among bank employees in Dhaka, Bangladesh. METHODS: A cross-sectional analysis was conducted with bank employees who worked in sedentary settings. The Eriksen subjective health complaints scale was used to measure the eight items of musculoskeletal health complaints (MHCs), and RTDs were measured using principal component analysis using variables commute time, distance, and traffic congestion experience to work. The association between RTDs and MHCs was identified using a multilevel model after adjusting potential confounders. RESULTS: A total of 628 employees (mean[SD] age, 36.1[7.0] years; 254[40.5%] women) participated in the study. Among the employees, the one-month prevalence of MHCs was 57.2%. The highest prevalence of MHCs was low-back pain (36.6%), followed by neck pain (22.9%) and upper-back pain (21.2%). Also, 136(21.7%) employees reported long-RTDs in commuting workplace and 81% of them had MHCs. The multilevel analysis identified that long-RTDs had a significant relationship with MHCs (adjusted odds ratio, AOR = 10.20, 95%CI = 5.41-16.91). Private transportation commuters reported 70% reduced odds of MHCs (AOR = 0.30, 95%CI = 0.15-0.59) and walking or bicycling commuters had 84% fewer MHCs (AOR = 0.16, 95%CI = 0.10-0.28) compared to public bus commuters. CONCLUSIONS: Sedentary employees with long-RTDs reported increased MHCs, emphasizing the importance of including musculoskeletal exercise in office facilities. Findings of this study also highlight the need for a sound public transportation system in Dhaka city.

Association between daily commute and subjective health complaints among the office workers in an urban community.

PURPOSE: To determine the prevalence of subjective health complaints (SHC) among metropolitan office commuters and to investigate the relationship between SHC and socio-demographic, commuting, and work-related factors. METHODS: The participants in this cross-sectional study were 628 full-time bank employees in Dhaka. One-month prevalence was determined using the SHC inventory scale. Internal consistency was determined using factor analysis. The discrepancy between socio-demographic and SHC was summarized using descriptive analysis. To discover factors related to SHC, random logistic regression intercept models were employed. RESULTS: Sadness (54.0 percent), low back pain (36.6 percent), anxiety (34.2 percent), pseudo-neurological disorders (26.6 percent), and musculoskeletal pains (20.2%) were the most common health complaints. The relationship between traffic congestion and SHCs was found to be statistically significant (p = 0.001) for the majority of complaints. According to multilevel analysis, long-distance office commuters were 7.29 times more likely than short-distance commuters to suffer from musculoskeletal pains (AOR = 7.29, 95% CI = 3.58-15.21). Furthermore, we discovered that long-distance commuters were 2.72 times more likely to complain about flu (AOR = 2.72, 95% CI = 1.22-6.27), 1.56 times more likely to complain about pseudo-neurological problems (AOR = 1.56, 95% CI = 0.84-2.92), and 1.88 times more likely to complain about gastrointestinal problems (AOR = 1.88, 95% CI = 0.69-5.41). CONCLUSION: In Dhaka, we found a high prevalence of health concerns among full-time bank personnel. A significant prevalence of health complaints was related to traffic congestion, long commutes, and use of public transportation. Reducing daily commuting time, switching modes of transportation, and avoiding traffic congestion could help to alleviate the burden of health concerns experienced by regular office commuters.

Immediate impact of stay-at-home orders to control COVID-19 transmission on mental well-being in Bangladeshi adults: Patterns, Explanations, and future directions.

OBJECTIVE: We aim to evaluate the immediate impacts of COVID-19 stay-at-home orders on the mental well-being of Bangladeshi adults. We recruited 1404 healthy adults following the Bangladesh government's lockdown announcement. A questionnaire comprising the Warwick Edinburgh Mental Well-being Scale was used to define mental health. RESULTS: The overall mean score for well-being was 42.4, indicating that 51.9% of adults suffered from poor mental health. And within that 48% of males and 57% of females were depressed. The mean scores for government workers, unemployed workers, and business employees were 45.1, 39.6, and 39.5, respectively. Confounding adjustments in multivariable linear regression models revealed that married women, unemployed and business communities, and individuals returning to villages were heavily depressed. Stay-at-home orders had significant repercussions on mental health and created a gender disparity in depression among adults. Suggestions include promoting mental health for women, unemployed, and business individuals. Married women need to be taken into special consideration as their mental well-being is worse. Older people (50 years of age and over) reported a high day-to-day variation in their mental health. These results should be factored in when discussing the mental health of adults and communities to cope with quarantine.

Genotype-phenotype correlation of ß-lactamase-producing uropathogenic Escherichia coli (UPEC) strains from Bangladesh.

Escherichia coli is a pathogen commonly encountered in clinical laboratories, and is capable of causing a variety of diseases, both within the intestinal tract (intestinal pathogenic strains) and outside (extraintestinal pathogenic E. coli, or ExPEC). It is associated with urinary tract infections (UTIs), one of the most common infectious diseases in the world. This report represents the first comparative analysis of the draft genome sequences of 11 uropathogenic E. coli (UPEC) strains isolated from two tertiary hospitals located in Dhaka and Sylhet, Bangladesh, and is focused on comparing their genomic characteristics to each other and to other available UPEC strains. Multilocus sequence typing (MLST) confirmed the strains belong to ST59, ST131, ST219, ST361, ST410, ST448 and ST4204, with one of the isolates classified as a previously undocumented ST. De novo identification of the antibiotic resistance genes blaNDM-5, blaNDM-7, blaCTX-M-15 and blaOXA-1 was determined, and phenotypic-genotypic analysis of virulence revealed significant heterogeneity within UPEC phylogroups.

Draft Genome Sequence of Bacillus cereus TN10, a Chromium-Resistant and -Reducing Strain Isolated from Tannery Effluent.

Here, we present the draft genome sequence of Bacillus cereus strain TN10, which exhibited chromium resistance and chromium-reducing ability. The whole-genome sequence analysis of strain TN10 will help us to understand its genetic factors involved in the bioremediation of Cr6.

Prevalence and associated occupational factors of low back pain among the bank employees in Dhaka City.

OBJECTIVE: Low back pain (LBP) is one of the common health problems among full-time office employees that causes absenteeism from work. The aim of the study is to identify the association between occupational factors and LBP among full-time bank employees in Dhaka City. MATERIALS AND METHODS: We conducted a cross-sectional study involving 593 full-time bank employees who were engaged in sedentary works. The 1-month complaint of LBP was measured using a subscale of subjective health complaints inventory. Multivariable logistic models were performed to identify variables related to LBP, and a random forest technique was performed to determine the top five important variables. RESULTS: The 1-month prevalence for LBP was found to be 36.6% among the bank employees, and the prevalence was the highest (64.3%) for the 51- to 59-year-old age group. The regression analysis indicates that respondents from both agegroups, 41-50 years (OR = 2.00, 95% confidence interval [CI] = 1.10-3.69) and 51-59 years age groups (OR = 5.14, 95% CI = 2.05-13.64), are significantly associated with LBP. Furthermore, obesity (OR = 2.06, 95% CI = 1.01-4.21), and prolong working hours (>9 hours) (OR = 1.42, 95% CI = 1.01-2.0) are positively associated with LBP. The top five important variables for LBP identified by random forest technique are: age, length of employment, prolong office hours, presence of chronic illness, and physical activity. CONCLUSION: LBP is highly prevalent in full-time bank employees. The occupational factors, including the length of employment (>10 years) and long working hours, play a significant role in developing LBP among bank employees. Moreover, several factors, including age, chronic illness, obesity, and physical activity, should be taken into account in the prevention of LBP in bank employees.

Age and gender-specific antibiotic resistance patterns among Bangladeshi patients with urinary tract infection caused by Escherichia coli.

BACKGROUND: In Bangladesh, treatment for urinary tract infection has become increasingly difficult due to antibiotic resistance. In addition, the prescription of age and gender-specific drugs is still far from being practiced in Bangladesh. We are examining trends of antibiotic resistance per age and gender in patients with urinary tract infection (UTI) caused by the most frequent agent, Escherichia coli. METHODS: We determined the resistance of 1663 E. coli isolates obtained from urine cultures. A sensitivity study using the Kirby-Bauer method was carried out to identify the antibiotic resistance trends. RESULTS: Imipenem with 1.9% resistance of all isolates found to be the lowest percentage of resistance. Meropenem (2.8%), amikacin (2.8%), colistin (2.9%), and nitrofurantoin (15.8%) showed low resistance percentages. The sensitivity analysis suggests that age and gender (area under curve = 0.67) should be taken into consideration to prescribe amikacin. The increasing odds ratios (OR) by age groups suggest that amikacin is a less effective agent for older patients with UTIs. Moreover, nitrofurantoin (OR = 1.45, 95% confidence interval (CI) = 1.07-1.95) and colistin (OR = 2.09, CI = 1.13-3.76) were less effective against isolates obtained from males compared to isolates obtained from females. Meropenem was effective against bacteria obtained from all age groups and genders. On the other hand, efficacy of imipenem was lower in isolates obtained from adults older than 40 years (OR: 0.44 for < = 18 years, OR = 0.47 for 19-40 years, OR = 0.86 for 41-60 years; reference: > = 61 years). CONCLUSION: In Bangladesh, meropenem, imipenem, amikacin, colistin, and nitrofurantoin are suitable therapeutic alternatives against urinary tract pathogens. Among the oral agents, amikacin, colistin, and nitrofurantoin should be prescribed, taking consideration of age and gender. These results will assist physicians in prescribing effective primary care antibiotics for UTI patients and encouraging the implementation of health policies for a safe prescription of antibiotics.

Whole genome sequencing provides genomic insights into three Morganella morganii strains isolated from bovine rectal swabs in Dhaka, Bangladesh.

Morganella morganii, a gram negative, facultative anaerobic bacterium belonging to the Proteeae tribe of the Morganellaceae family, is an unusual opportunistic pathogen mainly responsible for nosocomial and urinary tract infections. While cattle have long been established as a source of a few zoonotic pathogens, no such data has been recorded for M. morganii despite its ubiquitous presence in nature and a number of animal hosts. In this study, draft genomes were produced of three M. morganii isolates from Bangladeshi cattle. The three isolates, named B2, B3 and B5, possessed an average genome size of 3.9 Mp, a GC% of ∼51% and pan and core genomes of 4637 and 3812 genes, respectively. All strains were bearers of the qnrD1 carrying plasmid Col3M and possessed roughly similar virulence profiles and prophage regions. The strains also carried genes that were unique when compared with other publicly available M. morganii genomes. Many of these genes belonged to metabolic pathways associated with adaptation to environmental stresses and were predicted in silico to be borne in genomic islands. The findings of this study expand on the current understanding of M. morganii''s genomic nature and its adaptation in cattle.

High-fat diet-induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation.

The main objective of this experiment was to determine the effects of yogurt supplementation on fat deposition, oxidative stress, inflammation and fibrosis in the liver of rats with high-fat (HF) diet-induced obesity. Male Wistar rats were used in this study and were separated into the following four different groups: the control, control + yogurt, high fat and high fat+ yogurt groups. The high fat groups received a HF diet for eight weeks. A 5% yogurt (w/w) supplement was also provided to rats fed the HF diet. Yogurt supplementation prevented glucose intolerance and normalized liver-specific enzyme activities in the HF diet-fed rats. Yogurt supplementation also significantly reduced the levels of oxidative stress markers in the plasma and liver of HF diet-fed rats. Moreover, inflammatory cell infiltration, collagen deposition and fibrosis in the liver of HF diet-fed rats were also prevented by yogurt supplementation. Furthermore, yogurt supplementation normalized the intestinal lining and brush border in HF diet-fed rats. This study suggests that yogurt supplementation potentially represents an alternative therapy for the prevention of metabolic syndrome in HF diet-fed rats.

Occupational factors and low back pain: a cross-sectional study of Bangladeshi female nurses.

BACKGROUND: The suffering from low back pain (LBP) is very common among nurses. The high prevalence rates of LBP are observed in many countries. Many back injuries are due to individual and work-related factors. Our aim is to investigate whether there is an association of occupational factors with LBP among the female nurses who are currently working in tertiary hospitals of Bangladesh. METHODS: We conducted a cross-sectional study with 229 female nurses from two selected tertiary hospitals in Bangladesh. Data was collected through face-to-face interview using a standard structured questionnaire on four different measures of LBP along with questions on socio-demographic, occupational factors, physical and psychological factors. RESULTS: Prevalence rates of LBP that lasted for at least 1 day, chronic LBP, intense pain and sought medical care because of LBP during the last 12 months were 72.9, 31.8, 24.4 and 36.2%, respectively. The multiple logistic regression analyses indicates that insufficient supporting staffs, overtime working hours and manual lifting in a working environment are associated with LBP. Besides, age and parity are found positively associated with chronic LBP. CONCLUSION: The prevalence of LBP among nurses in Bangladesh is high and should be actively addressed. Certain occupational factors play a key role in developing LBP among nurses. Nurses to patients ratio should be taken into consideration to reduce the occurrence of LBP among nurses employed in hospitals.