Advancing Multi-Ion Sensing with Poly-Octylthiophene: 3D-Printed Milker-Implantable Microfluidic Device.

On-site rapid multi-ion sensing accelerates early identification of environmental pollution, water quality, and disease biomarkers in both livestock and humans. This study introduces a pocket-sized 3D-printed sensor, manufactured using additive manufacturing, specifically designed for detecting iron (Fe2+), nitrate (NO3 -), calcium (Ca2+), and phosphate (HPO4 2-). A unique feature of this device is its utilization of a universal ion-to-electron transducing layer made from highly redox-active poly-octylthiophene (POT), enabling an all-solid-state electrode tailored to each ion of interest. Manufactured with an extrusion-based 3D printer, the device features a periodic pattern of lateral layers (width = 80 µm), including surface wrinkles. The superhydrophobic nature of the POT prevents the accumulation of nonspecific ions at the interface between the gold and POT layers, ensuring exceptional sensor selectivity. Lithography-free, 3D-printed sensors achieve sensitivity down to 1 ppm of target ions in under a minute due to their 3D-wrinkled surface geometry. Integrated seamlessly with a microfluidic system for sample temperature stabilization, the printed sensor resides within a robust, pocket-sized 3D-printed device. This innovation integrates with milking parlors for real-time calcium detection, addressing diagnostic challenges in on-site livestock health monitoring, and has the capability to monitor water quality, soil nutrients, and human diseases.

Functionalized Graphene-Based Biosensors for Early Detection of Subclinical Ketosis in Dairy Cows.

Precision livestock farming utilizing advanced diagnostic tools, including biosensors, can play a key role in the management of livestock operations to improve the productivity, health, and well-being of animals. Detection of ketosis, a metabolic disease that occurs in early lactation dairy cows due to a negative energy balance, is one potential on-farm use of biosensors. Beta-hydroxybutyrate (ßHB) is an excellent biomarker for monitoring ketosis in dairy cows because ßHB is one of the main ketones produced during this metabolic state. In this report, we developed a low-cost, Keto-sensor (graphene-based sensor) for the detection of ßHB concentrations in less than a minute. On this device, graphene nanosheets were layered onto a screen-printed electrode (SPE), and then, a stabilized enzyme (beta-hydroxybutyrate dehydrogenase, NAD+, and glycerol) was used to functionalize the graphene surface enabled by EDC-NHS conjugation chemistry. The Keto-sensor offers an analytical sensitivity of 10 nm and a limit of detection (LoD) of 0.24 nm within a detection range of 0.01 µm-3.00 mm. Spike testing indicates that the Keto-sensor can detect ßHB in serum samples from bovines with subclinical ketosis. The Keto-sensor developed in this study shows promising results for early detection of subclinical ketosis on farms.

Direct decoration of commercial cotton fabrics by binary nickel-cobalt metal-organic frameworks for flexible glucose sensing in next-generation wearable sensors.

Having a prime significance in diagonsing and predicting the dangerous symptoms of chronic diseases in the early stages, special attention has been drawn by wearable glucose-sensing platforms in recent years. Herein, modified commercial cotton fabrics, decorated with binary Ni-Co metal-organic frameworks (NC-MOFs) through a one-pot scalable hydrothermal route, were directly utilized as flexible electrodes for non-enzymatic glucose amperometric sensing. Glucose sensitivities of 105.2 µA mM-1 cm-2 and 23 µA mM-1 cm-2 were acheived within two distinct linear dynamic ranges of 0.04-3.13 mM and 3.63-8.28 mM, respectively. Receiving benefits from a remarkable glucose sensitivity behavior in co-existence of iso-structures and interferences, rapid response (4.2 s), and remarkable reproducibility and repeatability, NC-MOF-modified cotton fabric electrodes are imensilly promising for developing high-performance wearable glucose sensing platfroms. The sensing performance of fabricated electrodes was further investigated in human blood serum and saliva.