[Study on the chemical reaction of cytosine with 3,3',5,5'-tetrabromo-m-cresojsulphonphthalein].

The system of reaction for t he cytosine (Cy) and 3,3',5,5'-tetrabrono-m-cresojsulphonphthalein (XJFL) was studied in acid solution using UV spectra. The factors that affect the reaction, such as temperature, time and concentration of ion were determined. The maximum absorption peak of the complex appeared at t he wavelength 620 nm, the molar absorptivity was epsilon = 1.32 x 10(3) L x cm(-1) x mol(-1), the linear range of standard was 0-32 micro x mL(-1), and the maximum combining number was 16. The reaction mechanism, and the interaction of inorganic substance, biological substance, and surface active agent were studied.

[Study on the reaction of Lys-ZCN-CTMAB complex].

The interaction of lysozyme (Lys), O-{2-[alpha-(2-Hydroxy-5-Sulphophenlylazo)-benzylidene] hydrazino} benzoic acid (ZCN) and cetyltrimethy ammonicumbromide (CTMAB) was investigated by UV spectrophotometric method. The effect of temperature, time, and ion intensity in the buffer solution (pH 4.46) on reaction system was determined. Linear range of the reaction was 0-10 microg x mL(-1). The molar absorptivity was epsilon = 4.6164 x 10(5) L x cm(-1) x mol(-1). The reaction mechanism and interference of inorganic substance, biological substance and surface active agent to the reaction was investigated. Study system was established.