Variational approach for Stokes flow through a two-dimensional non-uniform channel.

A variational approach is proposed to study the Stokes flow in a two-dimensional non-uniform channel. By using the stationarity of the Lagrangian, the Euler-Lagrange equations are established which leads to a simple set of ordinary differential equations to provide an estimate for the average pressure drop explicitly in terms of the channel shape function. The results for the pressure drop show an excellent agreement with the second-order extended lubrication theory. A higher-order formulation further improves the accuracy of the results for the pressure drop along the channel.

Whole-Slide Imaging (WSI) Versus Traditional Microscopy (TM) Through Evaluation of Parameters in Oral Histopathology: A Pilot Study.

Background: histopathology plays a pivotal role in clinical diagnosis, research, and medical education. In recent years, whole slide imaging (wsi) has emerged as a potential alternative to traditional microscopy for pathological examination. This study aims to provide a comprehensive comparison of wsi and traditional microscopy(tm) in various aspects of histopathology practice. Materials and Methods: In this study, total of 30 cases comprising of oral premalignant and malignant cases which were diagnostically challenging was considered from the archives of the institute for validation. The slides were scanned with slide scanner and were evaluated by histopathologists. The comparative parameters which were noted were diagnostic discordances, number of fields observed to reach the diagnosis and time taken. Results: The mean time taken by the pathologists to reach the diagnosis was significantly less in whole slide imaging technique. The average number of fields observed was higher by using wsi that too in a lesser time compared to tm, the results were found to be statistically significant with p=0.001.however the diagnostic disparity were seen to be maximum for verrucous lesions both in wsi and tm. Conclusion: wsi has facilitated the specialty with rapid mode of diagnosis in a more efficient and error less manner. It has also aided in case banking as well as research possibilities. Hence with the advent of telepathology it is very much necessary to get trained with wsi as early as possible so that the professionals can render correct diagnosis.

Utilizing Artificial Intelligence Application for Diagnosis of Oral Lesions and Assisting Young Oral Histopathologist in Deriving Diagnosis from Provided Features - A Pilot study.

Background: AI in healthcare services is advancing every day, with a focus on uprising cognitive capabilities. Higher cognitive functions in AI entail performing intricate processes like decision-making, problem-solving, perception, and reasoning. This advanced cognition surpasses basic data handling, encompassing skills to grasp ideas, understand and apply information contextually, and derive novel insights from previous experiences and acquired knowledge. ChatGPT, a natural language processing model, exemplifies this evolution by engaging in conversations with humans, furnishing responses to inquiries. Objective: We aimed to understand the capability of ChatGPT in solving doubts pertaining to symptoms and histological features related to subject of oral pathology. The study's objective is to evaluate ChatGPT's effectiveness in answering questions pertaining to diagnoses. Methods: This cross-sectional study was done using an AI-based ChatGPT application that provides free service for research and learning purposes. The current version of ChatGPT3.5 was used to obtain responses for a total of 25 queries. These randomly asked questions were based on basic queries from patient aspect and early oral histopathologists. These responses were obtained and stored for further processing. The responses were evaluated by five experienced pathologists on a four point liekart scale. The score were further subjected for deducing kappa values for reliability. Result & Statistical Analysis: A total of 25 queries were solved by the program in the shortest possible time for an answer. The sensitivity and specificity of the methods and the responses were represented using frequency and percentages. Both the responses were analysed and were statistically significant based on the measurement of kappa values. Conclusion: The proficiency of ChatGPT in handling intricate reasoning queries within pathology demonstrated a noteworthy level of relational accuracy. Consequently, its text output created coherent links between elements, producing meaningful responses. This suggests that scholars or students can rely on this program to address reasoning-based inquiries. Nevertheless, considering the continual advancements in the program's development, further research is essential to determine its accuracy levels in future versions.

Assessment of NIR-triggered PEG-coated NaGdF4:Tm3+/Yb3+bio-compatible upconversion nanoparticles for contrast enhancement in OCT imaging and optical thermometry.

In this investigation, we embarked on the synthesis of polyethylene glycol coated NaGdF4:Tm3+/Yb3+upconversion nanoparticles (UCNPs), aiming to assess their utility in enhancing image contrast within the context of swept source optical coherence tomography (OCT) and photo-thermal OCT imaging. Our research unveiled the remarkable UC emissions stemming from the transitions of Tm3+ions, specifically the1G4→3H6transitions, yielding vibrant blue emissions at 472 nm. We delved further into the UC mechanism, meticulously scrutinizing decay times and the nanoparticles' capacity to convert radiation into heat. Notably, these nanoparticles exhibited an impressive photo-thermal conversion efficiency of 37.5%. Furthermore, our investigations into their bio-compatibility revealed a promising outcome, with more than 90% cell survival after 24 h of incubation with HeLa cells treated with UCNPs. The nanoparticles demonstrated a notable thermal sensitivity of 4.7 × 10-3K-1at 300 K, signifying their potential for precise temperature monitoring at the cellular level.

Juvenile pemphigus vulgaris: Literature review and a rare case report.

Pemphigus vulgaris (PV) is a chronic autoimmune blistering disorder characterized by the loss of intraepithelial adhesion affecting the skin and mucous membranes, predominantly affects females in their fifth and sixth decades of life. Due to its rare occurrence in children and adolescents, there is often a delay in diagnosis and treatment in this age group. PV should always be considered in the differential diagnosis of oral ulcerative and vesiculobullous lesions in both children and adolescents.

NaYF4:Ho3+/Yb3+@NaGdF4 core@shell upconversion nanoparticles for contrast enhancement in bimodal in-vitro OCT imaging.

Contrast enhancement is explored in optical coherence tomography images using core NaYF4:Ho3+/Yb3+ and core@shell NaYF4:Ho3+/Yb3+@NaGdF4 nanoparticles. Under 980 nm excitation, core@shell nanoparticles exhibited 2.8 and 3.3 times enhancement at 541 nm and 646 nm emission wavelengths of Ho3+ ions compared to core nanoparticles. Photo-thermal conversion efficiencies were 32% and 20% for core and core@shell nanoparticles. In swept-source optical coherence tomography (SSOCT), core@shell nanoparticles have shown superior contrast, while in photo-thermal optical coherence tomography (PTOCT) core nanoparticles have excelled due to their higher photo-thermal conversion efficiency. The enhancement in contrast to noise ratio obtained is 58 dB. Comparative assessments of scattering coefficients and contrast-to-noise ratios were conducted, providing insights into nanoparticle performance for contrast enhancement in optical coherence tomography.

Edible oil based optical clearing for optical coherence tomography angiography imaging.

In optical imaging, optical clearing agents are commonly used to enhance the structural details of a sample. The current study investigates how to use it to improve the data obtained by an optical coherence tomography angiography system. A natural edible oil with no chemical base has been used for optical clearing. In-vivo testing on mice and humans yielded excellent optical clearing. Using computational techniques, the improvement in angiography signal caused by the optical clearing agent is investigated qualitatively and quantitatively. Compared to the control group, applying the edible oil-based optical clearing agent demonstrated improved vessel percentage and refined vascular signal intensity along depth.

Evaluating the relative influence of climate and human activities on recent vegetation dynamics in West Bengal, India.

Assessing the relative importance of climate change and human activities is important in developing sustainable management policies for regional land use. In this study, multiple remote sensing datasets, i.e. CHIRPS (Climate Hazard Group InfraRed Precipitation with Station Data) precipitation, MODIS Land Surface Temperature (LST), Enhanced Vegetation Index (EVI), Potential Evapotranspiration (PET), Soil Moisture (SM), WorldPop, and nighttime light have been analyzed to investigate the effect that climate change (CC) and regional human activities (HA) have on vegetation dynamics in eastern India for the period 2000 to 2022. The relative influence of climate and anthropogenic factors is evaluated on the basis of non-parametric statistics i.e., Mann-Kendall and Sen's slope estimator. Significant spatial and elevation-dependent variations in precipitation and LST are evident. Areas at higher elevations exhibit increased mean annual temperatures (0.22 °C/year, p < 0.05) and reduced winter precipitation over the last two decades, while the northern and southwest parts of West Bengal witnessed increased mean annual precipitation (17.3 mm/year, p < 0.05) and a slight cooling trend. Temperature and precipitation trends are shown to collectively impact EVI distribution. While there is a negative spatial correlation between LST and EVI, the relationship between precipitation and EVI is positive and stronger (R2 = 0.83, p < 0.05). Associated hydroclimatic parameters are potent drivers of EVI, whereby PET in the southwestern regions leads to markedly lower SM. The relative importance of CC and HA on EVI also varies spatially. Near the major conurbation of Kolkata, and confirmed by nighttime light and population density data, changes in vegetation cover are very clearly dominated by HA (87%). In contrast, CC emerges as the dominant driver of EVI (70-85%) in the higher elevation northern regions of the state but also in the southeast. Our findings inform policy regarding the future sustainability of vulnerable socio-hydroclimatic systems across the entire state.

Temperature-dependent transformation of microbial community: A systematic approach to analyzing functional microbes and biogas production.

The stability and effectiveness of the anaerobic digestion (AD) system are significantly influenced by temperature. While majority research has focused on the composition of the microbial community in the AD process, the relationships between functional gene profile deduced from gene expression at different temperatures have received less attention. The current study investigates the AD process of potato peel waste and explores the association between biogas production and microbial gene expression at 15, 25, and 35 °C through metatranscriptomic analysis. The production of total biogas decreased with temperature at 15 °C (19.94 mL/g VS), however, it increased at 35 °C (269.50 mL/g VS). The relative abundance of Petrimonas, Clostridium, Aminobacterium, Methanobacterium, Methanothrix, and Methanosarcina were most dominant in the AD system at different temperatures. At the functional pathways level 3, α-diversity indices, including Evenness (Y = 5.85x + 8.85; R2 = 0.56), Simpson (Y = 2.20x + 2.09; R2 = 0.33), and Shannon index (Y = 1.11x + 4.64; R2 = 0.59), revealed a linear and negative correlation with biogas production. Based on KEGG level 3, several dominant functional pathways associated with Oxidative phosphorylation (ko00190) (25.09, 24.25, 24.04%), methane metabolism (ko00680) (30.58, 32.13, and 32.89%), and Carbon fixation pathways in prokaryotes (ko00720) (27.07, 26.47, and 26.29%), were identified at 15 °C, 25 °C and 35 °C. The regulation of biogas production by temperature possibly occurs through enhancement of central function pathways while decreasing the diversity of functional pathways. Therefore, the methanogenesis and associated processes received the majority of cellular resources and activities, thereby improving the effectiveness of substrate conversion to biogas. The findings of this study illustrated the crucial role of central function pathways in the effective functioning of these systems.

Bacteria populating freshly appeared supraglacial lake possess metals and antibiotic-resistant genes.

Antibiotic resistance (AR) has been extensively studied in natural habitats and clinical applications. AR is mainly reported with the use and misuse of antibiotics; however, little is known about its presence in antibiotic-free remote supraglacial lake environments. This study evaluated bacterial strains isolated from supraglacial lake debris and meltwater in Dook Pal Glacier, northern Pakistan, for antibiotic-resistant genes (ARGs) and metal-tolerant genes (MTGs) using conventional PCR. Several distinct ARGs were reported in the bacterial strains isolated from lake debris (92.5%) and meltwater (100%). In lake debris, 57.5% of isolates harbored the blaTEM gene, whereas 58.3% of isolates in meltwater possessed blaTEM and qnrA each. Among the ARGs, qnrA was dominant in debris isolates (19%), whereas in meltwater isolates, qnrA (15.2%) and blaTEM (15.2%) were dominant. ARGs were widely distributed among the bacterial isolates and different bacteria shared similar types of ARGs. Relatively greater number of ARGs were reported in Gram-negative bacterial strains. In addition, 92.5% of bacterial isolates from lake debris and 83.3% of isolates from meltwater harbored MTGs. Gene copA was dominant in meltwater isolates (50%), whereas czcA was greater in debris bacterial isolates (45%). Among the MTGs, czcA (18.75%) was dominant in debris strains, whereas copA (26.0%) was greater in meltwater isolates. This presents the co-occurrence and co-selection of MTGs and ARGs in a freshly appeared supraglacial lake. The same ARGs and MTGs were present in different bacteria, exhibiting horizontal gene transfer (HGT). Both positive and negative correlations were determined between ARGs and MTGs. The research provides insights into the existence of MTGs and ARGs in bacterial strains isolated from remote supraglacial lake environments, signifying the need for a more detailed study of bacteria harboring ARGs and MTGs in supraglacial lakes.

Climate uncertainty and vulnerability of urban flooding associated with regional risk using multi-criteria analysis in Mumbai, India.

The study made a comprehensive effort to examine climatic uncertainties at both yearly and monthly scales, along with mapping flood risks based on different land use categories. Recent studies have progressively been engrossed in demonstrating regional climate variations and associated flood probability to maintain the geo-ecological balance at micro to macro-regions. To carry out this investigation, various historical remote sensing record, reanalyzed and in-situ data sets were acquired with a high level of spatial precision using the Google Earth Engine (GEE) web-based remote sensing platform. Non-parametric techniques and multi-layer integration methods were then employed to illustrate the fluctuations in climate factors alongside creating maps indicating the susceptibility to floods. The study reveals an increased pattern in LST (Land Surface Temperature) (0.03 °C/year), albeit marginal declined in southern coastal regions (-0.15 °C/year) along with uneven rainfall patterns (1.42 mm/year). Moreover, long-term LULC change estimation divulges increased trends of urbanization (16.4 km2/year) together with vegetation growth (8.7 km2/year) from 2002 to 2022. Furthermore, this inquiry involves numerous environmental factors that influence the situation (elevation data, topographic wetness index, drainage density, proximity to water bodies, slope, and soil properties) as well as socio-economic attributes (population) to assess flood risk areas through the utilization of Analytical Hierarchy Process and overlay methods with assigned weights. The outcomes reveal nearly 55 percent of urban land is susceptible to flood in 2022, which were 45 and 37 percent in 2012 and 2002 separately. Additionally, 106 km2 of urban area is highly susceptible to inundation, whereas vegetation also occupies a significant proportion (52 km2). This thorough exploration offers a significant chance to formulate flood management and mitigation strategies tailored to specific regions during the era of climate change.

Phase Asymmetry of Andreev Spectra from Cooper-Pair Momentum.

In analogy to conventional semiconductor diodes, the Josephson diode exhibits superconducting properties that are asymmetric in applied bias. The effect has been investigated in a number of systems recently, and requires a combination of broken time-reversal and inversion symmetries. We demonstrate a dual of the usual Josephson diode effect, a nonreciprocal response of Andreev bound states to a superconducting phase difference across the normal region of a superconductor-normal-superconductor Josephson junction, fabricated using an epitaxial InAs/Al heterostructure. Phase asymmetry of the subgap Andreev spectrum is absent in the absence of in-plane magnetic field and reaches a maximum at 0.15 T applied in the plane of the junction transverse to the current direction. We interpret the phase diode effect in this system as resulting from finite-momentum Cooper pairing due to orbital coupling to the in-plane magnetic field. At higher magnetic fields, we observe a sign reversal of the diode effect that appears together with a reopening of the spectral gap. Within our model, the sign reversal of the diode effect at higher fields is correlated with a topological phase transition that requires Zeeman and spin-orbit interactions in addition to orbital coupling.

High prevalence of antibiotic-resistant and metal-tolerant cultivable bacteria in remote glacier environment.

Studies of antibiotic-resistant bacteria (ARB) have mainly originated from anthropic-influenced environments, with limited information from pristine environments. Remote cold environments are major reservoirs of ARB and have been determined in polar regions; however, their abundance in non-polar cold habitats is underexplored. This study evaluated antibiotics and metals resistance profiles, prevalence of antibiotic resistance genes (ARGs) and metals tolerance genes (MTGs) in 38 ARB isolated from the glacier debris and meltwater from Baishui Glacier No 1, China. Molecular identification displayed Proteobacteria (39.3%) predominant in debris, while meltwater was dominated by Actinobacteria (30%) and Proteobacteria (30%). Bacterial isolates exhibited multiple antibiotic resistance index values > 0.2. Gram-negative bacteria displayed higher resistance to antibiotics and metals than Gram-positive. PCR amplification exhibited distinct ARGs in bacteria dominated by ß-lactam genes blaCTX-M (21.1-71.1%), blaACC (21.1-60.5%), tetracycline-resistant gene tetA (21.1-60.5%), and sulfonamide-resistant gene sulI (18.4-52.6%). Moreover, different MTGs were reported in bacterial isolates, including mercury-resistant merA (21.1-63.2%), copper-resistant copB (18.4-57.9%), chromium-resistant chrA (15.8-44.7%) and arsenic-resistant arsB (10.5-44.7%). This highlights the co-selection and co-occurrence of MTGs and ARGs in remote glacier environments. Different bacteria shared same ARGs, signifying horizontal gene transfer between species. Strong positive correlation among ARGs and MTGs was reported. Metals tolerance range exhibited that Gram-negative and Gram-positive bacteria clustered distinctly. Gram-negative bacteria were significantly tolerant to metals. Amino acid sequences of blaACC,blaCTX-M,blaSHV,blaampC,qnrA, sulI, tetA and blaTEM revealed variations. This study presents promising ARB, harboring ARGs with variations in amino acid sequences, highlighting the need to assess the transcriptome study of glacier bacteria conferring ARGs and MTGs.

Calcifications in oral carcinomas: Depicts diversity of calcium in cancer biology!

Calcifications in the soft tissues can be accidental findings during diagnostic procedures. Oral squamous cell carcinoma (OSCC) forms the major percentage of oral malignancies; calcifications are rare findings in OSCC. Calcifications are seen as a result of necrosis, chronic inflammations as well as degenerative changes and imbalances of the local calcium and phosphorous environment. The presence of calcifications can be a prognostic marker, hypothesizing that the influx of calcium from hard tissues into the soft tissues, can probably determine the invasive nature and the destructive characteristics of the carcinoma; hence, detecting calcifications can help us in predicting the prognosis and spread of the malignancy.

Real Scenario of Oral Cancer Awareness Sessions-A Narrative Viewpoint.

Oral cancer is one of the most common forms of cancer seen in Southeast Asia. Tobacco, betel nut, and slaked lime are the important constituents of betel quid; this is regularly consumed by the youth and elderly as their regular practice. To curb this oral cancer menace, there are numerous policies and pathways, which are adopted by government, local authorities, and institutions. Among the various policies, one of the easiest ways to reach out to masses is in form of screening camps and sessions. Oral cancer screening forms the most vital part of any dental check-up camps. Due to ignorance or lack of adequate knowledge about the deadly results of cancer, people often neglect these screening camps. This may attribute to various reasons that lead to such ignorance and failure of such free screening sessions.

ABO Blood Grouping and Rhesus Factor Determination From Dental Pulp Tissue: A Forensic Research.

Introduction Collection of blood samples from mass disaster victims and blood grouping is a challenging task. This can be attributed to various reasons. From the forensic odontology perspective, teeth and bones are one of the noteworthy remains that can be derived from such sites. Aims and objectives The aim of our study is to detect ABO blood groups and Rhesus (Rh) factor from extirpated pulp tissue of the extracted teeth at zero, three, and six months' time interval by absorption-elution technique. Materials and methods The study consisted of 90 freshly extracted teeth as suggested by a biostatistician. Thirty teeth were analyzed immediately and 60 teeth were stored in vials without any preservative at room temperature. The pulp tissue was extirpated and studied at zero months, three months, and six months to determine blood groups and Rh factors. The extraction socket blood was tested to identify the blood group of that patient and used as a control reference. The blood grouping was done at respective time periods through the absorption-elution method and matched with the control. Data were analyzed using Statistical Package for the Social Sciences (SPSS) version 24 software (IBM Corp., Armonk, NY). The chi-square test and Kruskal-Wallis test were done. A p-value < 0.05 was considered statistically significant. Results Pulp showed the highest sensitivity for blood groups at zero months but it could be identified up to six months, although the sensitivity and specificity gradually decreased. ABO blood grouping showed higher sensitivity than the Rh factor as time progressed. Conclusion In cases where teeth are the only remains in a forensic condition, the dental pulp can be an authentic source for blood group detection.

Water Quality Index Assessment of River Ganga at Haridwar Stretch Using Multivariate Statistical Technique.

The Ganges (Ganga) river contributes significant water resources for the ecology and economy, but it frequently encounters severe deterioration due to cumulative impact from upstream natural and anthropogenic variables. Knowledge and understanding of the dynamic behavior of such networks remain a significant challenge, particularly in the context of rising environmental pressures, such as climate change and industrialization, as well as constraints in both process and data understanding across geographies. An interdisciplinary approach is required to be developed to investigate the hydrogeochemical dynamics and anthropogenic sources influencing water quality in major river systems. The present study has been carried out to evaluate the characterization of river water quality in terms of the physico-chemical & bacteriological parameters. Also, the development of a water quality index (WQI) for Domestic (drinking) and Spiritual (bathing) usage is a part of the study. The water quality index has been developed using the Canadian Council of Ministers of the Environmental Water Quality Index (CCME WQI). The river's water quality index score in the present study lies in the range of 38.32 to 79.82, indicating the quality of water from fair to poor for drinking purposes. The highest water quality index value of 79.82 has been observed at Guru Kashnik Ghat, while the lowest WQI value of 38.32 has been observed at Har ki Pauri for drinking purposes. However, the water quality score for bathing purposes ranged from 71.04 to 91.22 thus signifying the quality of the water from fair to good for bathing purposes. The highest water quality index value of 91.22 has been assessed at Guru Kashnik Ghat, while the lowest WQI value of 71.04 has been assessed at Bhimgoda Barrage. The developed water indices assessment in the present study will be beneficial for society to provide a benchmark for the control of water pollution in River Ganga. These findings will support policymakers and stakeholders in addressing water quality issues in a more efficient and effective manner. The study also emphasizes the requirement for ongoing water quality monitoring and evaluation in order to guarantee the long-term well-being of the river and its ecosystems.

Infantile Hemangioma of the Upper Lip: Report of a Rare Case With a Brief Review of Literature.

Hamartomas are tumor-like abnormalities typified by the presence of cellular proliferation indigenous to the native site. However, hamartomas maintain growth cessation without the potential for further growth or malignant transformation. Hamartomas are commonly seen in the lungs, kidney, liver, and spleen and rarely occur in the orofacial region. Various hamartomatous oral lesions include hemangiomas, lymphangiomas, tori, exostosis, dens invaginatus, dens evaginatus, odontomas, nevi, and cherubism. Infantile hemangiomas are benign vascular tumors that emerge soon after birth and experience rapid growth within the first year. Oral hemangiomas affect up to 6.4% of infants and are more common on the ventral surface of the tongue, as opposed to oral vascular malformations, which are more prevalent on the lips. It also has a 3:1 female-to-male predominance. Afterward, their growth usually stabilizes and enters a prolonged, incomplete involution phase. Uncomplicated hemangiomas generally exhibit spontaneous resolution, whereas few can leave behind scars and telangiectasias on the external surface of the skin on which it occurs. Thus, lesions located in anatomically sensitive regions necessitate vigilant surveillance and treatment. This paper deals with an asymptomatic swelling of the upper lip in a four-year-old female child but with problems in aesthetics, speech, and feeding. A thorough history, clinical examination, positive diascopy, ultrasonography, and histopathology confirmed the diagnosis of infantile hemangioma.

Exploring the Effect of Size Variability on Efficiency of Upconversion Nanoparticles as Optical Contrast Agents.

The current works explores the optical contrast property and nanaotheranostic capabilities of the rare earth based Upconversion nanoparticles. Optical coherence tomography (OCT) has been used to explore the particles ability to increase contrast and improve signal intensity. Photo thermal OCT, a function extension of OCT has been used to gauge the photo thermal potential of the nanoparticles. The nanoparticles successfully improved contrast and information from the deeper layers of the sample. The particles also showed excellent capability for use as photo thermal agents.

Search for Ultralight Dark Matter with Spectroscopy of Radio-Frequency Atomic Transitions.

The effects of scalar and pseudoscalar ultralight bosonic dark matter (UBDM) were searched for by comparing the frequency of a quartz oscillator to that of a hyperfine-structure transition in ^{87}Rb, and an electronic transition in ^{164}Dy. We constrain linear interactions between a scalar UBDM field and standard-model (SM) fields for an underlying UBDM particle mass in the range 1×10^{-17}-8.3×10^{-13} eV and quadratic interactions between a pseudoscalar UBDM field and SM fields in the range 5×10^{-18}-4.1×10^{-13} eV. Within regions of the respective ranges, our constraints on linear interactions significantly improve on results from previous, direct searches for oscillations in atomic parameters, while constraints on quadratic interactions surpass limits imposed by such direct searches as well as by astrophysical observations.