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1.
Nat Methods ; 21(6): 974-982, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38622459

RESUMEN

The simultaneous measurement of three-dimensional (3D) genome structure and gene expression of individual cells is critical for understanding a genome's structure-function relationship, yet this is challenging for existing methods. Here we present 'Linking mRNA to Chromatin Architecture (LiMCA)', which jointly profiles the 3D genome and transcriptome with exceptional sensitivity and from low-input materials. Combining LiMCA and our high-resolution scATAC-seq assay, METATAC, we successfully characterized chromatin accessibility, as well as paired 3D genome structures and gene expression information, of individual developing olfactory sensory neurons. We expanded the repertoire of known olfactory receptor (OR) enhancers and discovered unexpected rules of their dynamics: OR genes and their enhancers are most accessible during early differentiation. Furthermore, we revealed the dynamic spatial relationship between ORs and enhancers behind stepwise OR expression. These findings offer valuable insights into how 3D connectivity of ORs and enhancers dynamically orchestrate the 'one neuron-one receptor' selection process.


Asunto(s)
Cromatina , Elementos de Facilitación Genéticos , Perfilación de la Expresión Génica , Neuronas Receptoras Olfatorias , Receptores Odorantes , Análisis de la Célula Individual , Animales , Neuronas Receptoras Olfatorias/metabolismo , Análisis de la Célula Individual/métodos , Receptores Odorantes/genética , Receptores Odorantes/metabolismo , Ratones , Perfilación de la Expresión Génica/métodos , Cromatina/genética , Cromatina/metabolismo , Genoma , Transcriptoma , ARN Mensajero/genética , ARN Mensajero/metabolismo
3.
Nat Commun ; 15(1): 1768, 2024 Feb 27.
Artículo en Inglés | MEDLINE | ID: mdl-38409079

RESUMEN

Extrachromosomal circular DNAs (eccDNAs) have emerged as important intra-cellular mobile genetic elements that affect gene copy number and exert in trans regulatory roles within the cell nucleus. Here, we describe scCircle-seq, a method for profiling eccDNAs and unraveling their diversity and complexity in single cells. We implement and validate scCircle-seq in normal and cancer cell lines, demonstrating that most eccDNAs vary largely between cells and are stochastically inherited during cell division, although their genomic landscape is cell type-specific and can be used to accurately cluster cells of the same origin. eccDNAs are preferentially produced from chromatin regions enriched in H3K9me3 and H3K27me3 histone marks and are induced during replication stress conditions. Concomitant sequencing of eccDNAs and RNA from the same cell uncovers the absence of correlation between eccDNA copy number and gene expression levels, except for a few oncogenes, including MYC, contained within a large eccDNA in colorectal cancer cells. Lastly, we apply scCircle-seq to one prostate cancer and two breast cancer specimens, revealing cancer-specific eccDNA landscapes and a higher propensity of eccDNAs to form in amplified genomic regions. scCircle-seq is a scalable tool that can be used to dissect the complexity of eccDNAs across different cell and tissue types, and further expands the potential of eccDNAs for cancer diagnostics.


Asunto(s)
ADN Circular , ADN , Masculino , Humanos , ADN Circular/genética , Cromosomas , Línea Celular , Oncogenes
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