RESUMEN
Syntheny and localization of the following genes in common shrew Sorex araneus were determined: isocitrate dehydrogenase 2 (IDH2), acid phosphatase 2 (ACP2), glutamine--pyruvate--oxo-acid transaminase (GPT), and inorganic pyrophosphatase (PP) on chromosome ik; adenylate kinases 1 and 3 (AK1 and AK3) on chromosome af; and enolase 1 (ENO1) on chromosome jl. Two genes were assigned to definite arms: aminoacylase 1 (ACY1) to arm p of chromosome mp and glutamic-oxaloacetic transaminase 1 (GOT1) to arm q of chromosome qr. Thus, 26 genes marking eight out of ten chromosomes are present now on the cytogenetic map of common shrew. These include previously described localizations.
Asunto(s)
Fosfatasa Ácida/genética , Alanina Transaminasa/genética , Mapeo Cromosómico , Isocitrato Deshidrogenasa/genética , Musarañas/genética , Adenilato Quinasa/genética , Animales , Línea Celular , Marcadores Genéticos , Pirofosfatasa Inorgánica , Cariotipificación , Fosfopiruvato Hidratasa/genética , Pirofosfatasas/genéticaRESUMEN
The repeated DNA sequence of wild ram (Ovis ammon) of 800 bp has been cloned. The blot-hybridization, in situ-hybridization, sequencing and computer analysis were used for the sequence analysis. It was shown that the cloned DNA is from 1.714 gm/cm3 repeated satellite DNA family. Fourteen highly homologous sequences were revealed in the nucleotide sequence databases. An analysis of their alignment revealed presence of two subfamilies (A and B). Average divergence of subfamily A. sequences (including the wild ram repeated sequence) from consensus is about 1%.
Asunto(s)
ADN/genética , ADN/aislamiento & purificación , Secuencias Repetitivas de Ácidos Nucleicos/genética , Ovinos/genética , Animales , Animales Salvajes , Secuencia de Bases , Células Cultivadas , Secuencia de Consenso , ADN Satélite/genética , Cabras/genética , Masculino , Datos de Secuencia Molecular , Alineación de Secuencia/métodos , Análisis de Secuencia de ADN/métodos , Homología de Secuencia de Ácido NucleicoRESUMEN
Monoclonal antibodies (MAs) were produced against glucose-6-phosphate dehydrogenase (G6PD) of two vole species--Microtus arvalis and M. subarvalis. The binding level of the MAs to G6PD in both species were almost the same, which suggested that these MAs may be specific for the antigenic determinants common to G6PD of these species. The MAs produced against the vole G6PD were used for its intracellular localization. The patterns obtained after staining cells with the use of MAs against G6PD were the same as those obtained after staining with the use of antibodies against F-actin. There was a good conformity between the results of light and electron microscopic immunoenzyme analyses with regard to the binding of MAs produced to the actin microfilaments. It is concluded that G6PD is closely associated with actin microfilaments of the cell cytoskeleton.
Asunto(s)
Fibroblastos/enzimología , Glucosafosfato Deshidrogenasa/metabolismo , Músculos/enzimología , Animales , Anticuerpos Monoclonales/aislamiento & purificación , Arvicolinae , Línea Celular , Células Cultivadas/enzimología , Células Cultivadas/ultraestructura , Electroforesis en Gel de Poliacrilamida/métodos , Fibroblastos/ultraestructura , Técnica del Anticuerpo Fluorescente , Glucosafosfato Deshidrogenasa/análisis , Glucosafosfato Deshidrogenasa/inmunología , Hibridomas/inmunología , Inmunización , Técnicas para Inmunoenzimas , Inmunohistoquímica , Ratones , Microscopía Inmunoelectrónica , Músculos/ultraestructura , RatasRESUMEN
Expression of X-linked genes for G6PD and alpha-GAL was studied in female interspecific hybrids of Microtus. The G6PD and alpha-GAL isozymes of Microtus arvalis were found to predominate in all cases when a species carrying a heterochromatin block on the X-chromosome served as one partner of hybridization and M. arvalis containing no heterochromatin block served as another. The proportions of G6PD and alpha-GAL parental forms were approx. equal in hybrid females when both species participating in hybridization contained heterochromatin blocks on X-chromosomes. Cytological analysis for revealing active and nonactive X-chromosomes on metaphase spreads of hybrid females supports the biochemical data. Non-random inactivation of X-chromosomes carrying the heterochromatin blocks in the interspecific hybrids with M. arvalis and a random one, when both parents contain heterochromatin blocks on the X-chromosomes are supposed to be the cause for the phenomenon observed. The study provided data supporting our previous hypothesis that heterochromatin affects the X-chromosome inactivation process in interspecific hybrid voles.