RESUMEN
Measurements of methane oxidation rates were made in southeastern Bering Sea water samples with [C]methane. The rate at which CO(2) evolved from samples exposed to one methane concentration was defined as the relative methane oxidation rate. Rate determinations at three methane concentrations were used to estimate methane oxidation kinetics. The rate constant calculated from the kinetics and the observed methane concentration in the same water sample were used to calculate an in situ methane oxidation rate and the turnover time. First-order kinetics were observed in essentially all experiments in which methane oxidation kinetics were measured. Relative methane oxidation rates were greater in waters collected at inshore stations than at the offshore stations and were greater in bottom samples than in surface samples. In most water samples analyzed, there was essentially no radioactivity associated with the cells. The resulting respiration percentages were therefore very high with a mean of >98%. These data suggest that most of the methane was used by the microflora as an energy source and that very little of it was used in biosynthesis. The relative methane oxidation rates were not closely correlated with methane concentrations and did not appear to be linked to either oxygen or dissolved inorganic nitrogen concentrations. However, there was a significant correlation with relative microbial activity. Our data suggest that the methane oxidizers were associated with the general microbial heterotrophic community. Since these organisms did not appear to be using methane as a carbon source, it is unlikely that they have been isolated and identified as methane oxidizers in the past.
RESUMEN
Unusual concentrations of dissolved two- to four-carbon alkanes were observed in the waters in Norton Sound in a localized area approximately 40 kilometers south of Nome, Alaska, in 1976. The hydrocarbons were identified in the near-bottom waters downcurrent for more than 100 kilometers from a sea-floor point source. Preliminary dynamic modeling estimates of the initial gas phase composition predict methanelethane and ethanelpropane ratios of 24 and 1.7, respectively, assuming the hydrocarbons were introduced by bubbles. The low ethanelpropane ratio is indicative of gas from a liquid petroleum source rather than from nonassociated or biogenic natural gas. Preliminary data on the structural geology of Norton Basin lend support to the interpretation based on the hydrocarbon plume. Unconformably truncated strata dip basinward from the seep locus; acoustic anomalies and numerous steeply dipping faults in the immediate vicinity of the seep are corroborating evidence that shallow gas- or petroleum-charged sediments and strata coincide with avenues for migration of mobile hydrocarbons to the sea floor. These factors, taken in concert with the sedimentological regime, the recent revision (increase) of basin depth estimates, and the highly localized hydrocarbon source, strongly suggest a thermogenic rather than a recent biogenic origin for these gaseous compounds.