RESUMEN
OBJECTIVE: An understanding of the role that self and body compassion may play as protective mechanisms in the context of psychological and physical health conditions is steadily increasing. The availability of research pertaining to their role in mitigating the health-related quality of life (HRQoL) impacts associated with endometriosis is limited. The current study examined the influence of self and body compassion on HRQoL in people diagnosed with endometriosis. METHODS: Individuals aged 18+ years (n = 318), assigned female at birth, with a self-reported diagnosis of symptomatic endometriosis completed an online cross-sectional survey. Participant demographics and endometriosis-related data was collected in addition to self and body compassion and HRQoL. Standard multiple regression analyses (MRA) were conducted to estimate the proportion of variance in HRQoL that can be accounted for by self and body compassion in endometriosis. RESULTS: Self and body compassion were both associated with higher HRQoL across all domains. However, when both self and body compassion were entered into a regression, only body compassion was significantly associated with HRQoL domains of physical wellbeing, bodily pain, vitality, social engagement and general HRQoL; self-compassion explained no unique variance. In the domain of emotional wellbeing, when both self and body compassion were entered into a regression, both were significantly associated, and explained unique variance. CONCLUSIONS: It is suggested that future psychological interventions for individuals living with endometriosis should emphasise building general self-compassionate abilities with a subsequent specific focus on strategies designed to improve body compassion.
Asunto(s)
Endometriosis , Calidad de Vida , Recién Nacido , Humanos , Femenino , Calidad de Vida/psicología , Empatía , Endometriosis/psicología , Estudios Transversales , EmocionesRESUMEN
The experimental objective was to determine the relative availability of endogenous Zn in soybean protein (SP) and cooked ground beef (GB) compared with the availability of Zn from ZnCO(3) for young swine. Crossbred pigs (4 trials with total n = 104) were weaned at an average age of 11 d, placed in metabolism cages, and allotted to 13 treatments. The basal semipurified negative control (NC) diet contained 2 mg of Zn/kg and wet-autoclaved spray-dried egg albumen (AEA) as the protein source. Reference diets were made by adding 3, 6, or 9 mg of Zn/kg from ZnCO(3) to the NC diet. The AEA in the NC diet was replaced with SP or GB to make diets with 3, 6, and 9 mg of Zn/kg from SP and GB. Positive control (PC) diets were made by adding 101 mg of Zn/kg from ZnCO(3) to the diets with 9 mg of Zn/kg from ZnCO(3), SP, or GB. Diets were mixed with deionized water and fed to appetite in gruel form every 3 h from 0800 to 2300 h. Pigs were weighed and blood samples were taken on d 7, 14, 21, and 28. Plasma concentrations of Cu, Fe, Zn, glucose, and urea N were determined. Total urine and fecal grab samples (Cr(2)O(3) in diets) were collected from d 7 to 14 and d 21 to 28 to determine Cu, Fe, Zn, and N apparent balance and energy utilization. On d 29, pigs were scored visually for parakeratosis and dosed orally with (65)Zn from ZnCl(2). Pigs were counted for whole body gamma radioactivity on d 29 and d 34 to 36, with Zn retention estimated by extrapolation to zero time. Pigs were killed on d 36 (NC on d 29). Samples of skin, tongue, and esophagus were taken for histological evaluation, and a tibia bone was excised for measurements and breaking strength. Pigs fed the diets with 9 mg Zn/kg from GB or ZnCO(3) were not different in Zn availability and retained about 70% Zn (apparent balance and (65)Zn) compared with 30% (P ≤ 0.05) for pigs fed the diet with 9 mg Zn/kg from SP. Also, pigs fed the diets with 9 mg Zn/kg from GB or ZnCO(3) had greater (P ≤ 0.05) growth performance, bone weight and breaking strength, % N retention, and % ME utilization, and smaller plasma urea N concentrations and parakeratosis scores (visual and histological) than pigs fed the diet with 9 mg Zn/kg from SP. Pigs fed the PC diets were not different in any response criteria. In conclusion, apparent Zn availability was not different from cooked GB and ZnCO(3), and Zn availability from cooked GB and ZnCO(3) was markedly greater than Zn availability from SP for young swine.
Asunto(s)
Alimentación Animal/análisis , Carne/análisis , Porcinos/fisiología , Zinc/farmacocinética , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Disponibilidad Biológica , Nitrógeno de la Urea Sanguínea , Carbonatos , Bovinos , Dieta/veterinaria , Óvulo/metabolismo , Proteínas de Soja , Destete , Zinc/sangre , Zinc/metabolismo , Compuestos de ZincRESUMEN
Globally, phosphorus (P) limits productivity of trees in many forests and plantations especially in highly weathered, acidic or calcareous profiles. Most trees form mycorrhizal associations which are prevalent in the organic and mineral soil horizons. This review critically examines mechanisms that enhance the acquisition of P by tree roots. Mycorrhizal roots have a greater capacity to take up phosphate (Pi) from the soil solution than non-mycorrhizal root tips. Factors that contribute to this include the extent of extraradical hyphal penetration of soil and the physiology and biochemistry of the fungal/soil and fungal/plant interfaces. Ectomycorrhizal (ECM) trees are likely to benefit from association with basidiomycetes that possess several high-affinity Pi transporters that are expressed in extraradical hyphae and whose expression is enhanced by P deficiency. To understand fully the role of these putative transporters in the symbiosis, data regarding their localization, Pi transport capacities and regulation are required. Some ECM fungi are able to effect release of Pi from insoluble mineral P through excretion of low-molecular-weight organic anions such as oxalate, but the relative contribution of insoluble P dissolution in situ remains to be quantified. How the production of oxalate is regulated by nitrogen remains a key question to be answered. Lastly, phosphatase release from mycorrhizas is likely to play a significant role in the acquisition of Pi from labile organic forms of P (Po). As labile forms of Po can constitute the major fraction of the total P in some tropical and temperate soils, a greater understanding of the forms of Po available to the phosphatases is warranted.
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Micorrizas/metabolismo , Fósforo/metabolismo , Árboles/metabolismo , Árboles/microbiología , Proteínas Portadoras/metabolismo , Proteínas Fúngicas/metabolismoRESUMEN
AIMS: The aim of this study was to develop a novel isolation technique using a mixture of Bacillus and Streptomyces phages to selectively isolate wax-utilizing non-streptomycete actinomycetes effective in ameliorating water repellency in a problem soil. METHODS AND RESULTS: Phages added to a soil suspension reduced the dominance of Bacillus and Streptomyces isolates and significantly increased the number of non-streptomycete actinomycetes on isolation plates. Promising isolates, grown on a medium containing beeswax as sole carbon source, were selected for application to water repellent soil. Their addition significantly reduced water repellency. CONCLUSIONS: Phage application significantly increased the isolation of non-streptomycete actinomycetes. Wax-utilizing isolates were found to significantly reduce water repellency in a problem soil. SIGNIFICANCE AND IMPACT OF THE STUDY: The phage technique can be used for the routine isolation of non-streptomycete actinomycetes. Beeswax medium can be used to selectively isolate wax-utilizing micro-organisms with the potential to ameliorate water repellency in soil.
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Actinobacteria , Microbiología del Suelo , Suelo , Agua , Actinobacteria/aislamiento & purificación , Actinobacteria/metabolismo , Actinobacteria/virología , Agar/química , Bacillus/clasificación , Bacillus/crecimiento & desarrollo , Bacillus/virología , Carbono/deficiencia , Ecología , Streptomyces/clasificación , Streptomyces/crecimiento & desarrollo , Streptomyces/virología , Ceras/químicaRESUMEN
Zinc deprivation results in decreased and cyclic food intake in rats. We determined the response of zinc-deprived rats to neuropeptide Y (NPY). In a preliminary experiment, rats were fed a low (-Zn; <1 mg/kg) or adequate zinc diet (+Zn; 100 mg/kg) for 4 days. NPY (5 or 10 microg) was then administered via an intracerebroventricular (ICV) cannula and food intake measured for 4 h. NPY stimulated food intake in all rats, but the difference in food intake due to zinc deprivation persisted. In a subsequent experiment, rats were fed the low zinc and adequate zinc diets for 4, 5 or 6 days. Food intake was suppressed in rats fed the low zinc compared to the adequate zinc diet on all of these days. When NPY (10 microg) was administered at the onset of the light cycle, the food intake was approximately 2.5-fold greater regardless of dietary zinc status, but the amount of food consumed by rats fed low zinc was approximately one-half the quantity consumed by NPY-stimulated zinc-adequate rats. NPY administered at the onset of dark failed to stimulate food intake in either dietary group although the total intake difference due to zinc status persisted. ICV administration of 5 nmol of zinc prior to NPY injection failed to correct the food intake response of the zinc-deficient rats. We conclude that the basis of the reduced food intake of zinc-deficient rats does not relate to NPY quantity or release, or to impairment of its signal transduction. There appears to be another undefined factor that limits food intake in zinc deficiency.
Asunto(s)
Ingestión de Alimentos/efectos de los fármacos , Neuropéptido Y/farmacología , Zinc/deficiencia , Animales , Inyecciones Intraventriculares , Masculino , Estado Nutricional , Ratas , Ratas Wistar , Zinc/administración & dosificaciónRESUMEN
The jcpk gene on mouse Chromosome 10 causes a severe, early onset form of polycystic kidney disease (PKD) when inherited in an autosomal recessive manner. In order to positionally clone this gene, high resolution genetic and radiation hybrid maps were generated along with a detailed physical map of the approximately 500-kb region containing the jcpk gene. Additionally, sixty-nine kidney-specific ESTs were evaluated as candidates for jcpk and subsequently localized throughout the mouse genome by radiation hybrid mapping analysis. Previous studies indicating non-complementation of the jcpk mutation and 67Gso, a new PKD translocation mutant had suggested that 67Gso represents a new allele of jcpk. Fluorescence in situ hybridization (FISH) analysis using key bacterial artificial chromosome clones from the jcpk critical region, refined the 67Gso breakpoint and provided support for the allelism of jcpk and 67Gso.
Asunto(s)
Mapeo Cromosómico , Enfermedades Renales Poliquísticas/genética , Animales , Etiquetas de Secuencia Expresada , Genes Recesivos , Marcadores Genéticos , Humanos , Ratones , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Ratones Mutantes , Datos de Secuencia MolecularRESUMEN
The present study investigates whether there is significant remobilization of (10)B previously loaded in the flag and penultimate leaves into the young, actively growing ear enclosed within the sheaths of flag and penultimate leaves. It also explores whether B transport into the enclosed ear declines when air humidity in the shoot canopy increases. After 5 d (10)B labelling during the period from early to full emergence of the flag leaf, the plants were transferred into nutrient solutions containing either 10 microM (11)B or no added B for 3 d. Regardless of the subsequent B supply levels to the roots, (10)B contents in the ear continued to increase by up to 5-fold 3 d after the end of (10)B supply in the nutrient solution. During these 3 d, the ear experienced a rapid increase in biomass. However, the majority of B in the ear during the 3 d treatment period was from the newly acquired (11)B from root uptake, rather than retranslocation of (10)B previously deposited in the leaves. By comparing the relative distribution of (10)B, Rb (xylem-to-phloem transfer marker) and Sr (xylem-marker) in the ear and the flag leaf, the distribution of (10)B resembled that of Rb more than Sr. Canopy cover treatment greatly suppressed leaf transpiration and decreased the amount of newly acquired (10)B in the flag leaf and the ear, but not in the upper stem segments. The results suggest that whilst the young ear was still fully enclosed within the leaf sheaths without any significant transpiration activity, B transport into the ear is predominantly dependent on the long-distance B transport in the xylem driven by leaf transpiration and, therefore, on concurrent B uptake from the roots.
Asunto(s)
Boro/metabolismo , Triticum/metabolismo , Transporte Biológico , Biomasa , Humedad , Hidroponía , Marcaje Isotópico , Estructuras de las Plantas/efectos de los fármacos , Estructuras de las Plantas/fisiología , Transpiración de Plantas/efectos de los fármacos , Transpiración de Plantas/fisiología , Rubidio/metabolismo , Estroncio/metabolismo , Distribución Tisular , Triticum/química , Triticum/efectos de los fármacos , Triticum/crecimiento & desarrolloRESUMEN
The concentration of plasma zinc is the generally accepted index of zinc status. Although low plasma zinc is an essential criterion of deficiency, alone it is inadequate. To supplement this index, we sought to determine the first limiting biochemical defect in animals fed zinc-deficient diets and concluded that the limiting function is associated with a posttranslational change in plasma membrane proteins. Among the signs of zinc deficiency in rats is a bleeding tendency associated with failure of platelet aggregation, a phenomenon that correlates with impaired uptake of Ca(2+) when stimulated. Zinc-deficient guinea pigs exhibit signs of peripheral neuropathy, and their brain synaptic vesicles exhibit impaired Ca(2+) uptake when they are stimulated with glutamate. Red cells from zinc-deficient rats show increased osmotic fragility associated with decreased plasma membrane sulfhydryl concentration. Both phenomena are readily reversed (2 d) by dietary zinc repletion. Volume recovery is dependent on Ca-dependent K channels and the sulfhydryl redox state. Both the impaired aggregation and calcium uptake of zinc-deficient platelets are corrected by in vitro incubation of blood with glutathione. Considering the fact that plasma membranes from several cell types show impaired function that is associated with a decreased rate of calcium uptake, it is postulated that a defect in calcium channels is the first limiting biochemical defect in zinc deficiency. The calcium uptake defect and consequent impaired second-messenger function likely results from an abnormal sulfhydryl redox state in the membrane channel protein.
Asunto(s)
Membrana Celular/efectos de los fármacos , Membrana Celular/fisiología , Proteínas de la Membrana/metabolismo , Zinc/fisiología , Animales , Canales de Calcio/efectos de los fármacos , Canales de Calcio/metabolismo , Membrana Celular/metabolismo , Enfermedades Carenciales/tratamiento farmacológico , Enfermedades Carenciales/fisiopatología , Dieta , Estado Nutricional , Fragilidad Osmótica , Oxidación-Reducción , Compuestos de Sulfhidrilo/metabolismo , Zinc/sangre , Zinc/deficiencia , Zinc/metabolismoRESUMEN
This review is concerned with the role of zinc in the function of the nervous system with emphasis on the effects of dietary zinc deprivation. Zinc deficiency leads to several pathological signs, some of which occur within a few days in growing animals deprived of zinc. Depletion eventually leads progressively to more severe behavioral changes, to abnormal stance, and to peripheral neuropathy. The nervous system contains many essential zinc metalloproteins that are highly stable, i.e. have high zinc association constants. These proteins, which include enzymes, transcription factors and storage proteins, do not become limiting during incipient zinc deficiency. It is likely that other, yet unidentified, zinc dependent proteins become limiting within a few days after animals are deprived of dietary zinc and lead to behavioral changes such as decreased and cyclic feeding. One candidate for the first limiting zinc component of nerve tissue is the "chelatable" zinc pool. Another is the plasma membrane zinc pool; the latter pool is decreased in zinc deficient rat erythrocytes, leading to oxidation of protein thiol groups and malfunction of that membrane. While the defective biochemical mechanism(s) that leads to the signs of deficiency is not entirely clear, there is evidence that synaptosomes from zinc deficient guinea pigs do not take up calcium normally when stimulated. It is postulated that the most vulnerable zinc pool serves a protective role against oxidative damage to plasma membrane proteins including the proteins involved with calcium channels. If so, the first limiting role of zinc is protective rather than catalytic or that of a neurotransmitter.
RESUMEN
Zinc deficiency leads to pathological signs that are related to impaired function of plasma membrane proteins. The purpose of this study was to assess the effect of dietary zinc status on the sulfhydryl (SH) content of erythrocyte plasma membranes and erythrocyte function. Three experiments were performed. In the first, immature male rats were fed for 21 d either a low-zinc (<1.0 mg/kg) diet free choice (-ZnAL), an adequate-zinc (100 mg/kg) diet free choice (+ZnAL), or the adequate-zinc diet limited to the intake of -ZnAL pair-mates (+ZnPF). Tail blood was sampled to measure osmotic fragility and SH concentration of erythrocyte membrane proteins. The zinc-deficient rats were then repleted for 2 d and erythrocytes assayed for fragility and SH content. In the second experiment blood was sampled at 3-d intervals to determine the time course of change in fragility and SH concentration. In the third experiment the SH concentration of erythrocyte band 3 protein and the binding of zinc to isolated plasma membranes were measured. SH concentration decreased from approximately 75 nmol/mg protein to 68 nmol/mg protein during 21 d of depletion and returned to control level within 2 d of repletion. There was an inverse relationship between osmotic fragility and SH concentration of erythrocyte membrane proteins. Maximal decrease in SH occurred within 6 d of consuming the low-zinc diet. The SH content of band 3 protein isolated from deficient rats was also significantly lower than that of pair-fed controls (45 vs. 51 nmol/mg protein). The zinc-binding affinity of plasma membrane proteins tended to be decreased by zinc deficiency. In summary, low-zinc status lowers the plasma membrane SH concentration, and the decreased reducing potential is inversely related to osmotic fragility, and presumably, with impaired volume recovery of erythrocytes.
Asunto(s)
Proteína 1 de Intercambio de Anión de Eritrocito/metabolismo , Proteínas de la Membrana/metabolismo , Fragilidad Osmótica , Compuestos de Sulfhidrilo/metabolismo , Zinc/deficiencia , Animales , Enfermedades Carenciales/sangre , Enfermedades Carenciales/metabolismo , Dieta , Masculino , Proteínas de la Membrana/análisis , Estado Nutricional , Ratas , Ratas Wistar , Compuestos de Sulfhidrilo/análisis , Zinc/sangre , Zinc/metabolismoRESUMEN
Growth failure in zinc-deficient animals is associated with decreased DNA synthesis; zinc deprivation of 3T3 cells, by use of diethylenetrinitrilopentaacetate (DTPA), impairs thymidine incorporation when the cells are stimulated with fetal bovine serum (FBS). The purpose of this study was to determine the step of cell cycle progression that is affected by zinc deprivation. Swiss murine 3T3 cells were cultured for 3 d in complete media and then for 2 d in low serum media. Cells were then placed in serum-free media and stimulated in sequence with platelet-derived growth factor (PDGF; 3 h), epidermal growth factor (EGF; 0.5 h) and insulin-like growth factor-I (IGF-I; 16 h). The combination of growth factors stimulated thymidine incorporation to the same extent as 10% FBS, and DTPA or EDTA (0.6 mmol/L) inhibited thymidine incorporation. Inhibition was prevented by addition of zinc, but not calcium, iron or cadmium (0.4 mmol/L). When DTPA was present during all stages with no addition of zinc, or zinc added during the competency-priming (PDGF and EGF) step, the IGF-I step, or both steps, the zinc effect occurred at the IGF-I step. Zinc addition 4 h before the measurement of thymidine incorporation had no ameliorative effect, but the presence of zinc during the prior 12 h increased incorporation. Thus zinc exerts its major effect on DNA synthesis during the IGF-I stimulatory phase of the cell cycle. The total zinc concentration of 3T3 cells treated with DTPA for 16 h was not different from that of untreated cells; hence only a small compartment of the cell is affected by DTPA.
Asunto(s)
Células 3T3/efectos de los fármacos , Células 3T3/metabolismo , Quelantes/toxicidad , ADN/biosíntesis , Factor I del Crecimiento Similar a la Insulina/farmacología , Ácido Pentético/toxicidad , Timidina/metabolismo , Zinc/deficiencia , Animales , Células Cultivadas , Factor de Crecimiento Epidérmico/farmacología , Ratones , Factor de Crecimiento Derivado de Plaquetas/farmacología , Zinc/farmacología , Zinc/fisiologíaRESUMEN
Depletion of zinc inhibits growth in animals and proliferation of cultured cells. Additionally, zinc can serve as an antioxidant protecting many compounds, including proteins, from oxidation. Regulation of cell division also involves insulin-like growth factor type I (IGF-I) and its receptor, especially during late G1 phase, allowing progression of the cell to S phase with subsequent DNA synthesis. We examined the effects of zinc depletion from the culture media of Swiss 3T3 cells on the cell cycle and IGF-I receptor expression. Cells were exposed to reduced fetal bovine serum concentrations to induce growth arrest, then returned to normal fetal bovine serum concentrations with the divalent cation chelator diethylenetriamine pentaacetic acid. Reducing the fetal bovine serum concentration did not induce quiescence in the cells as previously suggested. Zinc depletion reduced the proliferative fraction (S and G2/M phases) of the cell cycle. The addition of glutathione to the zinc-depleted media partially returned the proliferative fraction to the control level. Fetal bovine serum deprivation reduced IGF-I receptor expression whereas the absence of zinc had little effect on receptor expression. We conclude that depletion of zinc from culture media inhibits 3T3 cell proliferation independent of insulin-like growth factor-I receptor expression, and part of this inhibition is due to the antioxidant capacity of this divalent cation.
Asunto(s)
Células 3T3/fisiología , Receptor IGF Tipo 1/metabolismo , Zinc/deficiencia , Animales , Bovinos , División Celular , Separación Celular/métodos , Quelantes/farmacología , Citometría de Flujo/métodos , Glutatión/farmacología , Ratones , Ácido Pentético/farmacología , Albúmina Sérica Bovina/farmacología , Zinc/metabolismoRESUMEN
This article encompasses my perception of, and experience in, an exciting segment of the trace element era in nutrition research: the role of zinc in the nutrition of animals and humans. Zinc has been a major player on the stage of trace element research, and it has left a trail that galvanized the attention of many researchers, including myself. It is ubiquitous in biological systems, and it plays a multitude of physiologic and biochemical functions. A brief historical overview is followed by a discussion of the contributions the work done in my laboratory has made toward understanding the physiological and biochemical functions of zinc. The effort of 40 years has led to the belief that one of zinc's major roles, and perhaps its first limiting role, is to preserve plasma-membrane function as regards ion channels and signal transduction. Although substantial knowledge has been gained relating to the importance of zinc in nutrition, much remains to be discovered.
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Fenómenos Fisiológicos de la Nutrición , Zinc/fisiología , Animales , Disponibilidad Biológica , Alimentos , Historia del Siglo XX , Humanos , Investigación/historiaRESUMEN
Zinc deficiency in rats results in impaired growth accompanied by decreased and cyclic food intake. These signs are associated with decreased plasma insulin-like growth factor-I (IGF-I), a major mediator of growth. The purpose of this study was to determine the relationship between decreased plasma IGF-I and the impairment of appetite and growth in zinc deficiency. Immature male rats were fed free choice a low zinc (<1 mg/kg) diet (-Zn) or a zinc adequate (100 mg/kg) control diet (+Zn). Plasma IGF-I concentrations were normalized in zinc-deficient rats by the following two methods: osmotic pump infusion of IGF-I (2.4 mg/kg body weight daily) and oral administration (50 mg/kg body weight twice daily) of the synthetic progestin, megestrol acetate (MA). Infusion of IGF-I for 8 d sustained plasma IGF-I concentrations in zinc-deficient rats at control levels but had no effect on either food intake or growth rate. MA administration for 8 d maintained the plasma IGF-I of deficient rats and significantly increased food intake. The early aspects of cyclic food intake were eliminated, and, after a few days, food intake of deficient rats given MA was not different than that of controls. MA increased food intake and fat deposition regardless of zinc status, but it had no effect on the growth rate of deficient rats. MA significantly decreased body weight of controls, uncoupling energy intake and gain. The results suggest that reduced food intake precedes the decreased plasma IGF-I concentration and that IGF-I is not responsible for the decreased growth and food intake of zinc-deficient rats. The appetite and growth impairment of zinc-deficient rats may arise from disrupted function of IGF-I receptors in the brain and peripheral tissues, but not from low circulating levels of IGF-I.
Asunto(s)
Estimulantes del Apetito/farmacología , Ingestión de Alimentos/efectos de los fármacos , Factor I del Crecimiento Similar a la Insulina/farmacología , Acetato de Megestrol/farmacología , Zinc/deficiencia , Tejido Adiposo , Animales , Composición Corporal , Dieta , Factor I del Crecimiento Similar a la Insulina/metabolismo , Masculino , Ratas , Ratas Wistar , Aumento de Peso/efectos de los fármacos , Zinc/administración & dosificaciónRESUMEN
Pichia pastoris is a yeast capable of expressing large amounts of some proteins. When expression vectors are introduced into P. pastoris, individual transformants typically express widely varying amounts of protein. Because clones expressing the highest level of protein occur infrequently during the transformation process, finding them can be very labor-intensive. We developed an immunological based filter screening method that rapidly detects transformants secreting large amounts of a heterologous protein. We have applied this method to the expression of a soluble trimeric form of CD40L, a molecule that regulates B-cell responses. Using this method, we identified transformants with one to 13 copies of the CD40L expression cassette. Maximum expression was obtained with clones containing eight or more copies of the expression cassette, and a clone with eight copies was selected for further analysis. High cell density fermentation of this clone using a mixed glycerol:methanol feed yielded 255 mg CD40L per liter of supernatant.
Asunto(s)
Glicoproteínas de Membrana/genética , Pichia/genética , Secuencia de Aminoácidos , Animales , Ligando de CD40 , Clonación Molecular , Fermentación , Expresión Génica , Vectores Genéticos , Humanos , Glicoproteínas de Membrana/biosíntesis , Glicoproteínas de Membrana/inmunología , Ratones , Datos de Secuencia Molecular , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/inmunologíaRESUMEN
Zinc-deficient guinea pigs develop a peripheral neuropathy characterized by abnormal posture and gait, hyperesthesia, slowed motor nerve conduction velocity (MNCV), and decreased sciatic nerve Na,K-ATPase activity. This study was designed to further investigate longitudinally the morphophysiologic features of the neuropathy. Weanling guinea pigs were fed a low-zinc (<1 mg/kg) diet ad libitum (-ZnAL), an adequate-zinc (100 mg/kg) diet ad libitum (+ZnAL), or the adequate diet restricted in intake ((+ZnRF). Electrophysiologic, morphologic, and biochemical parameters of peripheral nerves were examined at 2.5, 4.0, and 5.5 weeks. Serum zinc was significantly lower by 2.5 weeks and growth rate reduced by 4 weeks in -ZnAL animals. Postural abnormalities were first obvious at 4 weeks, although MNCVs were significantly slower in zinc-deficient animals at all time intervals. The conduction of sensory impulses, as measured by spinal cord somatosensory evoked potentials (sSSEP), was significantly slower in the -ZnAL animals at 5.5 weeks. Examination of teased preparations and histologic sections of sciatic nerves at 5.5 weeks revealed no degenerative lesions or differences in density of myelinated fibers (MF). The size frequency distribution of MF in all groups was unimodal, with a trend toward smaller myelinated nerve fibers in -ZnAL and +ZnRF animals. Sciatic nerve Na,K-ATPase activity in the -ZnAL animals was significantly reduced after 4 weeks of zinc deprivation. At 5.5 weeks, nerve concentrations of myo-inositol, glucose, fructose, and sorbitol were significantly decreased in -ZnAL animals compared with the +ZnRF and +ZnAL controls. The peripheral neuropathy associated with acute zinc deficiency is a parenchymatous axonal disorder characterized by slowed motor and sensory nerve impulse conduction and reduction in nerve Na,K-ATPase activity and nerve concentrations of simple sugars and their metabolites.
Asunto(s)
Enfermedades del Sistema Nervioso Periférico/fisiopatología , Zinc/deficiencia , Animales , Glucemia/metabolismo , Peso Corporal , Plexo Braquial/química , Plexo Braquial/patología , Carbohidratos/análisis , Potenciales Evocados Somatosensoriales/fisiología , Femenino , Fructosa/sangre , Cobayas , Inositol/análisis , Estudios Longitudinales , Masculino , Conducción Nerviosa/fisiología , Enfermedades del Sistema Nervioso Periférico/patología , ATPasa Intercambiadora de Sodio-Potasio/metabolismo , Sorbitol/sangre , Médula Espinal/fisiopatología , Zinc/sangreRESUMEN
There are numerous measures of essential mineral element status, including growth rate, tissue and physiological fluid concentrations, enzyme concentrations and activities, chemical balance and mobilizable stores. For the adult human, blood and its specific nutrient concentrations provide a useful but frequently inadequate index. The first limiting biochemical system should provide the most valid index, but in many cases it is unknown or not readily measured. Chemical balance and mobilizable stores provide valid measures but are difficult to determine. Two indices are infinitely more valuable than one and should be determined if possible. More research is needed to establish valid indicators of nutritional status for mineral elements.
Asunto(s)
Minerales , Modelos Biológicos , Necesidades Nutricionales , Estado Nutricional , Oligoelementos , Adulto , Alimentos Fortificados , Homeostasis , Humanos , Masculino , Zinc/metabolismoRESUMEN
Cardiopulmonary bypass surgical techniques that allow a surgeon to operate on the infant's heart use an extracorporeal circuit consisting of a pump, oxygenator, arterial and venous reservoirs, cannulae, an arterial filter, and tubing. The extracorporeal technique currently used in infants and neonates is sometimes associated with neurologic damage. We are developing a modified cardiopulmonary bypass system for neonates that has been tested in vitro and in one animal in vivo. Unlike other extracorporeal circuits which use steady flow, this system utilizes pulsatile flow, a low prime volume (500 ml) and a closed circuit. During in vitro experiments, the pseudo patient's mean arterial pressure was kept constant at 40 mmHg and the extracorporeal circuit pressure did not exceed a mean pressure of 200 mmHg. In our single in vivo experiment, the primary objective was to determine whether physiologic pulsatility with a 10 F (3.3 mm) aortic cannula could be achieved. The results suggest that this is possible.
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Puente Cardiopulmonar , Flujo Pulsátil/fisiología , Animales , Diseño de Equipo , Circulación Extracorporea/efectos adversos , Humanos , Técnicas In Vitro , Lactante , Recién Nacido , Membranas Artificiales , Consumo de Oxígeno/fisiología , Oxigenadores de Membrana/normas , Cloruro de PoliviniloRESUMEN
Zinc deficiency in guinea pigs decreases glutamate-stimulated calcium uptake in cortical synaptosomes. Glutamate not only stimulates calcium uptake but also potentiates the binding of the drug dizocilpine (MK-801) to an internal site of the N-methyl-D-aspartate receptor/calcium channel, a subtype of the glutamate receptor. The purpose of this study was to determine whether the effect of zinc deficiency on calcium uptake by glutamate-stimulated synaptosomes is related to N-methyl-D-aspartate receptor number or function, as measured by MK-801 binding. Immature guinea pigs consumed a low zinc (< 1 mg/kg) diet ad libitum or an adequate zinc (100 mg/kg) diet, either ad libitum or restricted to maintain weight similar to that of the low zinc animals. Binding of MK-801 to cortical membranes was measured first in the presence of saturating concentrations of glutamate or N-methyl-D-aspartate in combination with glycine. Zinc deficiency significantly reduced the concentration of MK-801 binding sites (20%) regardless of the potentiating agonist used, but had no effect on binding affinity. The binding of MK-801 in response to 1, 10 and 100 mumol/L glycine, in the presence of 100 nmol/L glutamate, was then measured and found to be significantly reduced (12%). The results suggest that zinc deficiency decreases the number of functional N-methyl-D-aspartate receptor/channels in cortical membranes, probably because of impaired channel opening.