RESUMEN
Groundwater discharge (GD) is an important component of the water budget in large urban areas with high water demands. Radon is a routinely used groundwater tracer in mass-balances for evaluating GD to surface water bodies. The diffusion of 222Rn from aquifers' sediments parameter is important for GD's assessments. Sediment equilibration experiments were employed with various sediment and rock materials, including sand, granite, gneiss and loess that constitute the Pampeano Aquifer (PA) in the Buenos Aires Province, Argentina. These experiments allowed the 222Rn concentration determination in pore fluids at secular equilibrium and to examine the factors affecting the magnitude of radon 222Rn emanation from the materials under study. We found that radon emanation decreases in a power function (R2 = 0.9, n = 6) with the particle size of the tested PA sediment and rock materials. Based on our results, loess sediments with the smallest particle size and the largest particle surface area have the highest radon emanation. This strongly suggests that these two parameters are the parameters that govern the radon diffusive fluxes' magnitude in the PA. On the other hand, we found that PA basement rocks, primarily granite and gneiss, showed an exhalation rate of radon of 8.1 ± 0.81 Bqâm-2âh-1 and 13.2 ± 1.32 Bqâm-2âh-1. These rates are two orders of magnitude higher than loess sediments (0.3 ± 0.1 Bqâm-2âh-1), owning to the higher natural content of radon's parent isotopes from the 238U natural decay series. These high radon levels are consistent with currently available radon concentrations measured in groundwater in contact with the PA basement rock formations. This study demonstrates the importance of considering site-specific aquifer properties in the radon diffusive fluxes when utilizing radon as a groundwater tracer in hydrological studies. This is the first quantitative study that examines the aquifer characteristics affecting radon emanations in this large hydrogeological system.
Asunto(s)
Agua Subterránea , Monitoreo de Radiación , Radón , Radón/análisis , Arena , Dióxido de Silicio , AguaRESUMEN
Sirtuin 2 (SIRT2), a nicotinamide adenine dinucleotide (NAD)-dependent deacetylase expressed by oligodendrocytes (OLs), the myelin-producing cells of the central nervous system (CNS), is markedly up-regulated during active myelination (Li et al. (2007) J Neurosci 27:2606-2616; Southwood et al. (2007) Neurochem Res 32:187-195; Werner et al. (2007) J Neurosci 27:7717-7730). SIRT2 is a component of the myelin proteome and is severely reduced in the Plp1 knockout mouse brain, in which both proteolipid protein (PLP) and DM20 are absent (Werner et al. (2007) J Neurosci 27:7717-7730). The mechanisms that regulate SIRT2 expression in OLs and myelin remain to be investigated. We report for the first time that the expression of SIRT2 is regulated by the QKI-dependent pathway and this effect is mediated through selective regulation of PLP. In the homozygous quakingviable (qk(v) /qk(v) ) mutant mouse that harbors QKI deficiency in OLs (Bockbrader and Feng (2008) Future Neurol 3:655-668; Ebersole et al. (1996) Nat Genet 12:260-265; Hardy et al. (1996) J Neurosci 16:7941-7949), PLP, but not DM20 mRNA, was selectively down-regulated and SIRT2 protein was severely reduced whereas SIRT2 mRNA expression was unaffected. Expression of the cytoplasmic isoform QKI6 in OLs (Zhao et al. (2006) J Neurosci 26:11278-11286) rescued SIRT2 expression in the qk(v) /qk(v) mutant concomitantly with restoration of PLP expression. Moreover, SIRT2 protein is diminished in myelin tracts and compact myelin of the PLP-ISEdel mutant brain, in which PLP protein but not DM20 is selectively reduced (Wang et al. (2008) Exp Neurol 214:322-330). In contrast, SIRT2 expression and its cellular function in regulating process complexity are not affected by the absence of PLP in PLP-ISEdel non-myelinating OLs. Collectively, our results indicate that the abundance of SIRT2 in myelin is dependent on PLP, but not DM20.
Asunto(s)
Encéfalo/citología , Proteína Proteolipídica de la Mielina/metabolismo , Vaina de Mielina/metabolismo , Oligodendroglía/metabolismo , Proteínas de Unión al ARN/metabolismo , Transducción de Señal/fisiología , Sirtuina 2/metabolismo , Factores de Edad , Animales , Animales Recién Nacidos , Diferenciación Celular , Citometría de Flujo , Regulación del Desarrollo de la Expresión Génica , Proteínas Fluorescentes Verdes , Ratones , Ratones Endogámicos C57BL , Ratones Quaking , Ratones Transgénicos , Mutación , Proteína Proteolipídica de la Mielina/genética , ARN Mensajero , Proteínas de Unión al ARN/genética , Receptores del Factor Natriurético Atrial/genética , Transducción de Señal/genética , Sirtuina 2/genéticaRESUMEN
We present a new simple approach for automated, non-destructive measurement of the alpha-emitting radium isotopes ((223)Ra, (224)Ra, and (226)Ra) in water based on the emanation of their respective radon daughters ((219)Rn, (220)Rn, and (222)Rn). The method combines the high adsorption uptake of MnO(2) Resin for radium (K(d)=2.4 x 10(4)ml/g) over a wide pH range with the simplicity of the activity registration using a commercial radon-in-air analyzer (RAD7, DURRIDGE Company, Inc). Radium is first adsorbed onto the MnO(2) Resin by passing a water sample through the resin packed in a gas-tight glass cartridge. The same cartridge is then connected to the radon analyzer via a simple tubing system to circulate air through the resin and a drying system. The efficiency of the proposed system is determined by running standards prepared in the same manner. Our results indicate that the efficiency for (226)Ra is >22% if both (218)Po and (214)Po counts are collected. This is comparable with typical efficiencies for alpha spectrometry but with much less sample preparation. We estimate that an MDA of 0.8 pCi/L for (226)Ra may be obtained with this new approach using a 1L water sample and less than 4h of counting.