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Genet Mol Res ; 12(2): 1411-20, 2013 Apr 26.
Artículo en Inglés | MEDLINE | ID: mdl-23661464

RESUMEN

Pathogenesis-related proteins (PRs) are among the defense mechanisms of plants that work as an important barrier to the development of pathogens. These proteins are classified into 17 families according to their amino acid sequences, serology, and/or biological or enzyme activity. The present study aimed to identify PRs associated with the pathosystem of Capsicum baccatum var. pendulum: Pepper yellow mosaic virus (PepYMV). Forty-five-day-old plants from accession UENF 1624, previously identified as resistant to PepYMV, were inoculated with the virus. Control and infected leaves were collected for analysis after 24, 48, 72, and 96 h. The inoculated and control plants were grown in cages covered with anti-aphid screens. Proteins were extracted from leaf tissue and the presence of ß-1,3-glucanase, chitinase, peroxidase, and lipid transport protein was verified. No difference was observed between the protein pattern of control and infected plants when ß-1,3-glucanase, chitinase, and lipid transport protein were compared. However, increased peroxidase expression was observed in infected plants at 48 and 72 h after inoculation, indicating that this PR is involved in the response of resistance to PepYMV in C. baccatum var. pendulum.


Asunto(s)
Capsicum/enzimología , Capsicum/virología , Resistencia a la Enfermedad , Virus del Mosaico/fisiología , Peroxidasa/metabolismo , Enfermedades de las Plantas/virología , Capsicum/genética , Resistencia a la Enfermedad/genética , Activación Enzimática , Interacciones Huésped-Patógeno , Peroxidasa/genética , Enfermedades de las Plantas/genética
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