Effect of magnetized water on blood indices and histomorphometric parameters of intestinal sections of Japanese quail.

A two-way experimental design was used to demonstrate the physiological effects of magnetized water and sex on blood indices and histomorphometric parameters of Japanese quail intestine sections. Red blood cell count (RBCs), packed cell volume (PCV), hemoglobin concentration (Hb), thrombocytes, white blood cell count (WBCs), and WBC differentiation were investigated. A total of 450 unsexed Japanese quail were randomized into three groups (45/replicate; 3 replicates; 135/group). As a monitoring group, the first group was given untreated tap water to drink. The two others were consumed magnetized water that were subjected to an electrical magnetic field with a power of 1 Tesla (10,000 Gauss) and 2 Tesla (20,000 Gauss), respectively. The treatments had a significant (p ≤ 0.05) effect on thrombocytes and Hb. Sex showed significant (p ≤ 0.05) differences for RBCs and PCV at 42 days of age. At different ages, significant effects were observed on histomorphometric parameters of the Japanese quail intestinal tract. It may be inferred that the influence of magnetized water, up to 1 Tesla, was positive on the haematological and histomorphometric parameters of the Japanese quail intestinal tract by augmenting the haematological measurements, which were within a normal range and increasing the surface area of the villus.

Changes in cell proliferation kinetics during organogenesis and differentiation of the brain and optic cup in the chick embryo.

The kinetics of cell proliferation on during early ontogenesis of the brain and formation of the eye was studied in the chick embryo. Kinetic parameters--the mitotic index (MI), proliferative activity (P), the duration of mitosis (tm) and the generation time (T)--were measured in various regions of the brain and eye cup, using colchicine as a stathmokinetic agent. It was found that MI could be taken as an indicator of P during early development. In stage 11 it is also clear that the shortest T occurs in areas of rapid proliferation. By stage 12, although the generation time in the various regions studied differs, P and MI are approximately the same. A short tm is not always restricted to areas with a high P. P is affected by variation of T rather than by tm or time of any other parameter of the cycle. With increasing age, tm values decrease and T increase, probably owing to the progress of differentiation during embryonic development.