The family myth in nursing care for children in psychological distress.

This is a theoretical study aimed at reflecting on the contribution of the concept of family myth to nursing care for children in psychological distress. It is methodologically structured around three topics: the importance of the family in caring for children; the perspective of family-centered nursing care for children in psychological distress; and the contribution of the understanding of family myth to nursing care for children in psychological distress. The following dialectic is considered: the family, considered by current literature to be a harmonious unit, also triggers family conflicts that can be the cause of psychological suffering. The concept of family myth emerges as a possible theoretical anchor for nursing care for children in psychological distress, as it allows nurses to consider the signifiers that mark the child's psychological structure and construct their symptoms. Uncovering the place that the family assigns to the child enables nurses to help them construct and elaborate their own place as a subject in their subjectivity.

Collagen modifications predictive of lymph node metastasis in dogs with carcinoma in mixed tumours.

Introduction: Mixed tumours in the canine mammary gland are the most common histological type in routine diagnosis. In general, these neoplasms have a favourable prognosis that does not evolve into metastatic disease. However, some cases develop into lymph node metastases and are associated with worse patient survival rates. Methods: Here is a retrospective study of 46 samples of primary mixed tumours of the canine mammary gland: 15 cases of benign mixed tumours (BMT), 16 cases of carcinoma in mixed tumours without lymph node metastasis (CMT), and 15 cases of carcinomas in mixed tumours with lymph node metastasis (CMTM). In addition, we selected 23 cases of normal mammary glands (NMT) for comparison. The samples were collected from biopsies performed during nodulectomy, simple mastectomy, regional mastectomy, or unilateral/bilateral radical mastectomy. We used multiphoton microscopy, second harmonic generation, and two-photon excited fluorescence, to evaluate the characteristics of collagen fibres and cellular components in biopsies stained with haematoxylin and eosin. We performed Ki67, ER, PR, and HER-2 immunostaining to define the immunophenotype and COX-2. We showed that carcinomas that evolved into metastatic disease (CMTM) present shorter and wavier collagen fibres as compared to CMT. Results and discussion: When compared to NMT and BMT the carcinomas present a smaller area of fibre coverage, a larger area of cellular coverage, and a larger number of individual fibres. Furthermore, we observed a correlation between the strong expression of COX-2 and a high rate of cell proliferation in carcinomas with a smaller area covered by cell fibres and a larger number of individual fibres. These findings highlight the fundamental role of collagen during tumour progression, especially in invasion and metastatic dissemination.

[Intermittent fasting: effects in diverse clinical settings]. / Ayuno intermitente: efectos en diversos escenarios clínicos.

Introduction: Introduction: intermittent fasting plans propose to limit food intake during specific periods as nutritional therapeutic strategies to treat different metabolic conditions in various clinical entities. However, the heterogeneity between each context of intermittent fasting could generate different results in metabolic parameters. Objective: to evaluate the clinical application of intermittent fasting and to discern whether it offers advantages over other traditional strategies. Methods: structured questions were formulated (PICO), and the methodology followed the guidelines established by the PRISMA 2020 statement. The search was conducted in different databases (PubMed, Cochrane Library and Google Scholar). Results: we found 3,962 articles, of which 56 were finally included; 3,906 articles that did not directly or indirectly answer the structured questions were excluded. Conclusions: compared to conventional diets, the various AI schemes do not generate advantages or disadvantages in terms of weight loss and lipid profile, although in the alternate-day variant there are greater insulin reductions than those observed in the continuous energy restriction. The heterogeneity of the interventions, the populations studied, the comparators, the results, and the type of design make it impossible to extrapolate the effects found in all clinical scenarios and generalize the recommendations.

Introducción: Introducción: los esquemas de ayuno intermitente (AI) proponen limitar la ingestión de alimentos durante periodos específicos. Se han propuesto como estrategia dietoterapéutica para tratar distintas condiciones metabólicas en diversos padecimientos, sin embargo, la heterogeneidad entre cada contexto de ayuno intermitente pudiera generar diferentes resultados en parámetros metabólicos. Objetivo: evaluar la aplicación clínica del ayuno intermitente y discernir si ofrece ventajas sobre otras estrategias tradicionales. Métodos: se formularon preguntas estructuradas (PICO) y la metodología se apegó a las guías establecidas por la declaración PRISMA 2020. Se realizó una búsqueda de literatura científica en las plataformas de PubMed, Cochrane Library y Google Scholar. Resultados: se encontraron 3.962 artículos, de los cuales se incluyeron finalmente 56, eliminando 3.906 trabajos que no contestaban directa o indirectamente las preguntas estructuradas. Conclusiones: frente a las dietas convencionales, los diversos esquemas de AI no generan ventajas ni desventajas en cuanto a pérdida de peso o perfil lipídico, aunque en la variante a días alternos se producen mayores reducciones de insulina a las observadas en la restricción energética continua. La heterogeneidad de las intervenciones y poblaciones estudiadas, así como los comparadores, los desenlaces y el tipo de diseño imposibilitan extrapolar los efectos a todos los escenarios clínicos y generalizar las recomendaciones.

The family myth in nursing care for children in psychological distress / El mito familiar en los cuidados de enfermería a niños con malestar psíquico / O mito familiar no cuidado de enfermagem à criança em sofrimento psíquico

ABSTRACT This is a theoretical study aimed at reflecting on the contribution of the concept of family myth to nursing care for children in psychological distress. It is methodologically structured around three topics: the importance of the family in caring for children; the perspective of family-centered nursing care for children in psychological distress; and the contribution of the understanding of family myth to nursing care for children in psychological distress. The following dialectic is considered: the family, considered by current literature to be a harmonious unit, also triggers family conflicts that can be the cause of psychological suffering. The concept of family myth emerges as a possible theoretical anchor for nursing care for children in psychological distress, as it allows nurses to consider the signifiers that mark the child's psychological structure and construct their symptoms. Uncovering the place that the family assigns to the child enables nurses to help them construct and elaborate their own place as a subject in their subjectivity.

RESUMEN Se trata de un estudio teórico dirigido a reflexionar sobre la contribución del concepto de mito familiar a los cuidados de enfermería a niños con sufrimiento psicológico. Metodológicamente se estructura en torno a tres temas: la importancia de la familia en el cuidado de los niños; la perspectiva de los cuidados de enfermería centrados en la familia para niños con sufrimiento psicológico; y la contribución de la comprensión del mito familiar a los cuidados de enfermería a niños con sufrimiento psicológico. Se plantea la dialéctica: la familia, considerada por la literatura actual como una unidad armoniosa, también desencadena conflictos familiares que pueden ser causa de sufrimiento psicológico. El concepto de mito familiar emerge como anclaje teórico para los cuidados de enfermería a los niños en malestar psicológico, ya que permite a las enfermeras considerar los significantes que marcan la estructura psicológica del niño y construyen sus síntomas. Desvelar el lugar que la familia asigna al niño permite a las enfermeras ayudarle a construir y elaborar su propio lugar como sujeto en su subjetividad.

RESUMO Trata-se de um estudo teórico com o objetivo de refletir sobre a contribuição do conceito de mito familiar para o cuidado de enfermagem à criança em sofrimento psíquico, sendo metodologicamente estruturado em três tópicos: importância da família no cuidado de crianças; perspectiva do cuidado de enfermagem centrado na família de crianças em sofrimento psíquico; e contribuição do entendimento de mito familiar para o cuidado de enfermagem à criança em sofrimento psíquico. Considera-se a dialética: a família, tida pela literatura atual como unidade harmônica, também desencadeia conflitos familiares que podem ser a causa de sofrimentos psíquicos. O conceito de mito familiar surge como possibilidade de ancoragem teórica ao cuidado de enfermagem à criança em sofrimento psíquico ao permitir que o enfermeiro considere os significantes que marcam a estruturação psíquica da criança e constroem seu sintoma. Desvendar o lugar que a família destina à criança viabiliza ao enfermeiro auxiliá-la a construir e elaborar um local próprio enquanto sujeito em sua subjetividade.

Processo de enfermagem e relação interpessoal: uma reflexão sobre o cuidado em saúde mental / Proceso de enfermería y relación interpersonal: una reflexión sobre el cuidado en salud mental / Nursing process and interpersonal relationship: a reflection on mental health care

Objetivo: refletir a possibilidade de articulação entre Processo de Enfermagem e fases da relação interpessoal e suas implicações no cuidado em saúde mental. Método: estudo de reflexão teórica, qualitativo, alinhado a visão e expertise das pesquisadoras junto ao referencial teórico da relação interpessoal. Resultados: a reflexão desenvolveu-se em três eixos sendo eles Processo de Enfermagem - um cuidado clínico possível; Relação interpessoal - uma teoria que sustenta o cuidado de enfermagem na saúde mental e Cuidado em saúde mental e as implicações do Processo de Enfermagem orientado pelas fases da relação interpessoal. Considerações finais: a relação interpessoal inserida no Processo de Enfermagem possibilita as intervenções, por meio da empatia, escuta, esclarecimento e encorajamento que ocorre no setting da consulta de enfermagem. A clínica da enfermagem em saúde mental compreende as fases da relação interpessoal inseridas discretamente no Processo de Enfermagem, mitigando o dilema de seu uso aliado a processos relacionais no cuidado de enfermagem.

Objetivo: reflejar la posibilidad de articulación entre Proceso de Enfermería y fases de la relación interpersonal, y sus implicaciones en el cuidado en salud mental. Método: estudio de reflexión teórica, cualitativo, alineado con la visión y experiencia de las investigadoras junto al referencial teórico de la relación interpersonal. Resultados: la reflexión se desarrolló en tres ejes: Proceso de Enfermería - un cuidado clínico posible; Relación interpersonal - una teoría que sustenta el cuidado de enfermería en la salud mental y Cuidado en salud mental y las implicaciones del Proceso de Enfermería orientado por las fases de la relación interpersonal. Consideraciones finales: la relación interpersonal inserta en el Proceso de Enfermería posibilita las intervenciones, por medio de la empatía, escucha, esclarecimiento y estímulo que ocurre en el setting de la consulta de enfermería. La clínica de enfermería en salud mental comprende las fases de la relación interpersonal insertas discretamente en el Proceso de Enfermería, mitigando el dilema de su uso aliado a procesos relacionales en el cuidado de enfermería.

Objective: to reflect the possibility of articulation between Nursing Process and phases of interpersonal relationship, and its implications for mental health care. Method: study of theoretical, qualitative reflection, aligned with the vision and expertise of the researchers with the theoretical framework of interpersonal relationship. Results: the reflection was developed in three axes: Nursing Process - a possible clinical care; Interpersonal relationship - a theory that supports nursing care in mental health and mental health care and the implications of the Nursing Process guided by the phases of interpersonal relationship. Final considerations: the interpersonal relationship inserted in the Nursing Process enables interventions, through empathy, listening, clarification and encouragement that occurs in the nursing consultation setting. The nursing clinic in mental health comprises the phases of interpersonal relationship inserted discreetly in the Nursing Process, mitigating the dilemma of its use allied to relational processes in nursing care.

Relación entre resiliencia y conductas alimentarias de riesgo / Relationship between resilience and risk eating behaviors

Resumen: Los trastornos de la conducta alimentaria son de las enfermedades crónicas más frecuentes en adolescentes y mujeres jóvenes. En México se han encontrado porcentajes desde 5 hasta 18% de conductas alimentarias de riesgo en muestras de mujeres estudiantes universitarias (Unikel-Santoncini, et al., 2004). Por otro lado, la resiliencia se define como un constructo que incluye ciertos fenómenos que consisten en la adecuada adaptación del individuo ante situaciones traumáticas, privación extrema, amenazas graves o estrés elevado (González-Arratia, 2008, en Camacho-Valadez, 2016). Esta investigación examina la relación entre la resiliencia y las conductas alimentarias de riesgo por medio de la aplicación de los instrumentos EAT-26 y RESI-M a 201 mujeres mexicanas, con un promedio de edad de 22 años. Se realizó un análisis de correlación de Producto Momento de Pearson, el cual demostró que, aquellas mujeres con mayores niveles de resiliencia mostraban menos conductas alimentarias de riesgo. Se propone realizar el mismo estudio con poblaciones más jóvenes, así como con participantes de sexo masculino.

Abstract: Eating disorders are one of the most frequent chronic diseases in adolescents and young women. In Mexico, percentages from 5 to 18% of risk eating behaviors have been found in samples of female university students (Unikel-Santoncini, et al., 2004). On the other hand, resilience is defined as a construct that includes certain phenomena that consist of the individual's adequate adaptation to traumatic situations, extreme deprivation, serious threats, or high stress (González-Arratia, 2008, in Camacho Valadez, 2016). This research examines the relationship between resilience and risk eating behaviors through the application of the EAT-26 and RESI-M instruments to 201 Mexican women, with an average age of 22 years. A Pearson Product Moment correlation analysis was performed, which showed that those women with higher levels of resilience showed less risk eating behaviors. It's proposed to carry out the same study with younger populations, as well as with male participants.

Insulin resistance induced by long-term hyperinsulinemia abolishes the effects of acute insulin exposure on cell-surface nicotinic acetylcholine receptor levels and actin cytoskeleton morphology.

Using CHO-K1/A5 cells, a clonal cell line that robustly expresses adult muscle-type nicotinic acetylcholine receptor (nAChR), we explored whether insulin resistance in these mammalian cells affects cell-surface expression of the nAChR, its endocytic internalization, and actin cytoskeleton integrity. Acute nanomolar insulin stimulation resulted in a slow increase in nAChR cell-surface levels, reaching maximum levels at ∼1 h. Long periods of insulin incubation caused CHO-K1/A5 cells to become insulin resistant, as previously observed with several other cell types. Furthermore, long-term insulin treatment abolished the effects of short-term insulin exposure on cell-surface nAChR levels, suggestive of a desensitization phenomenon. It also affected the kinetics of ligand-induced nAChR internalization. Since the integrity of the cortical actin cytoskeleton affects nAChR endocytosis, we also studied the effects of long-term insulin treatment on this meshwork. We found that it significantly affected the cortical actin morphology of CHO-K1/A5 cells and the response of the actin cytoskeleton to a subsequent short-term insulin stimulus. Overall, the present results show for the first time the effects of insulin signaling on cell-surface nAChR expression and actin cytoskeleton-associated internalization.

Experience of hospitalization of the family with children and adolescents in psychological distress.

OBJECTIVE: To learn about the experience of families of children and adolescents in psychological distress facing hospitalization in a psychiatric inpatient unit of a general university hospital. METHOD: This is a qualitative phenomenological-social study, with data collection carried out from February to March 2022 through open interviews, analyzed using Alfred Schutz's framework with the construction of categories of meaning. RESULTS: Based on the analysis of eight interviews, it was possible to understand changes in the families' way of life, impacts on their routine, work, and social relationships. Their expectations were about the recovery of mental health and autonomy. CONCLUSION: This study allowed us to understand the experience of families faced with the hospitalization of children and adolescents in psychological distress and understand their members' needs. The need for implementation of care spaces that incorporate the relationship between the multidisciplinary team and the family is highlighted.

Neonatal overfeeding alters the functioning of the mesolimbic dopaminergic circuitry involving changes in DNA methylation and effects on feeding behavior.

Mesolimbic dopaminergic circuit is essential for food reward and motivational behaviors and can contribute to weight gain and obesity. Litter reduction is a classical model for studying the effects of neonatal overfeeding and overweight. Litters of Wistar rats were reduced to 4 pups/dam for small litter (SL) and 10 pups/dam for normal litter at postnatal day (PND) 4. Immediately after performing the feeding behavior tests, the animals were sacrificed in PND21 and PND90. The ventral tegmental area (VTA), Nucleus Accumbens Core (NAcC) and Shell (NAcSh) were isolated from frozen brain sections using the Palkovits micropunch technique. RNA and DNA were extracted from these areas, gene expression was measured by RT-qPCR and DNA methylation levels were measured by MSRM-qPCR technique. SL-PND21 animals presented increased expression levels of Tyrosine Hydroxylase and Dopamine Receptor D2 in VTA, decreased expression levels of dopamine active transporter (DAT) in VTA, and higher expression levels of DAT in NAcC. On the other hand, SL-PND90 animals showed decreased expression levels of Dopamine Receptor D1 and higher expression of DAT in NAcSh. These animals also evidenced impaired sensory-specific satiety. In addition, altered promoter methylation was observed at weaning, and remained in adulthood. This work demonstrates that neonatal overfeeding induces disruptions in the mesolimbic dopaminergic circuitry and causes alterations in feeding behavior from weaning to adulthood, suggesting that the neonatal period is critical for the normal development of dopaminergic circuit that impact on feeding behavior.

EEG coherence and power spectra during REM sleep related to melatonin intake in mild-to-moderate Alzheimer's disease: a pilot study.

It has been reported that melatonin diminishes rapid eye movement (REM) sleep latency in patients with Alzheimer's disease (AD). Pharmacological studies suggest that melatonin promotes prompt sleep installation through interaction with GABA receptors, and that it is associated with acute suppression of neural electrical activity. Nevertheless, melatonin's effects on electroencephalographic (EEG) activity related to REM sleep onset in AD patients have not been analyzed. Thus, in this pilot study we analyzed the effects of melatonin on EEG activity during the first episode of REM sleep in eight patients treated with 5-mg of fast-release melatonin.During a single-blind, placebo-controlled study, polysomnographic recordings were obtained from frontal, central, temporal, and occipital scalp derivations. REM sleep latency, as well as the relative power (RP) and EEG coherences of six EEG bands, were compared between the placebo and melatonin conditions.Results showed that melatonin intake in AD patients decreased REM sleep onset, and that this was associated with lower RP and coherence of the ß and γ EEG bands.The possibility that the inhibitory GABAergic pathways related to REM sleep generation are well-preserved in mild-to-moderate AD is discussed. We conclude that the short REM sleep onset related to melatonin intake in AD patients is associated with a significant decrease in both RP and EEG coherence, mainly in the fast frequencies.

Experience of hospitalization of the family with children and adolescents in psychological distress / Experiencia de la familia de niños y adolescentes en sufrimiento psicológico en la internación / Experiência da família de crianças e adolescentes em sofrimento psíquico diante da hospitalização

ABSTRACT Objective: To learn about the experience of families of children and adolescents in psychological distress facing hospitalization in a psychiatric inpatient unit of a general university hospital. Method: This is a qualitative phenomenological-social study, with data collection carried out from February to March 2022 through open interviews, analyzed using Alfred Schutz's framework with the construction of categories of meaning. Results: Based on the analysis of eight interviews, it was possible to understand changes in the families' way of life, impacts on their routine, work, and social relationships. Their expectations were about the recovery of mental health and autonomy. Conclusion: This study allowed us to understand the experience of families faced with the hospitalization of children and adolescents in psychological distress and understand their members' needs. The need for implementation of care spaces that incorporate the relationship between the multidisciplinary team and the family is highlighted.

RESUMEN Objetivo: Conocer la experiencia de familias de niños y adolescentes en sufrimiento psicológico ante la internación en una unidad de internación psiquiátrica de un hospital general universitario. Método: Estudio cualitativo fenomenológico-social, recolección de datos realizada de febrero a marzo de 2022 a través de entrevistas abiertas, analizadas utilizando el referencial de Alfred Schutz con la construcción de categorías de significado. Resultados: A partir del análisis de ocho entrevistas, fue posible comprender cambios en el modo de vida de las familias, impactos en su rutina, trabajo y relaciones sociales. Sus expectativas estaban puestas en la recuperación de la salud mental y la autonomía. Conclusión: Este estudio permitió comprender la experiencia de las familias ante la hospitalización de niños y adolescentes en sufrimiento psicológico y comprender las necesidades de sus miembros. Se destaca la necesidad de implementar espacios de atención que incorporen la relación entre el equipo multidisciplinario y la familia.

RESUMO Objetivo: Conhecer a experiência da família de crianças e adolescentes em sofrimento psíquico diante da hospitalização em uma unidade de internação psiquiátrica de um hospital geral universitário. Método: Estudo qualitativo fenomenológico-social, coleta de dados realizada nos meses de fevereiro a março de 2022 por meio de entrevistas abertas, analisadas sob referencial de Alfred Schutz com construção de categorias de significado. Resultados: Com base na análise de oito entrevistas, foi possível compreender mudanças no modo de vida das famílias, impactos na sua rotina, trabalho e relações sociais. Suas expectativas versaram sobre a recuperação da saúde mental e autonomia. Conclusão: Este estudo permitiu conhecer a experiência das famílias diante da hospitalização de crianças e adolescentes em sofrimento psíquico e compreender as necessidades de seus membros. Destaca-se a necessidade da implementação de espaços de cuidados que incorporem a relação entre a equipe multiprofissional e a família.

Percepções da equipe de enfermagem sobre cuidados de crianças e adolescentes internados com transtornos mentais / Percepciones del equipo de enfermería sobre la atención a niños y adolescentes hospitalizados con trastornos mentales / Nursing team's perceptions on care for children and adolescents hospitalized with mental disorders

Resumo Objetivo conhecer as percepções da equipe de enfermagem ante o cuidado de crianças e adolescentes com transtornos mentais internados. Método estudo qualitativo fenomenológico social cuja coleta de dados foi realizada com 12 membros das equipes de enfermagem por meio de entrevista fenomenológica. Resultados "Motivos porque" descritos em duas categorias: a percepção da equipe de enfermagem em face dos cuidados prestados e ações e cuidados da equipe de enfermagem da criança com transtornos mentais. "Motivos para" expressos na categoria: a recuperação da criança com transtorno mental e o seu retorno para casa. Conclusões e Implicações para a prática a possibilidade do estabelecimento da relação face a face entre equipe, família e criança como forma de apreender a situação biográfica e o acervo de conhecimento dos sujeitos envolvidos e, então, entender as suas reais necessidades e demandas promovem um cuidado integral. Através da fenomenologia social de Alfred Schutz, traz-se como implicação para a prática a possibilidade de compreender a percepção da equipe de enfermagem sobre a relevância da relação face a face no cuidado de crianças e adolescentes com transtornos mentais.

Resumen Objetivo conocer las percepciones del equipo de enfermería sobre los cuidados prestados a los niños y adolescentes hospitalizados con trastornos mentales. Método estudio cualitativo fenomenológico social cuya recogida de datos se realizó con 12 miembros de los equipos de enfermería mediante entrevista fenomenológica. Resultados "Motivos-por qué" se describen en dos categorías: la percepción del equipo de enfermería ante los cuidados prestados y las acciones y cuidados del equipo de enfermería del niño con trastornos mentales. "Motivos-para" expresadas en la categoría: la recuperación del niño con trastorno mental y su regreso a casa. Conclusiones e Implicaciones para la práctica La posibilidad de establecer una relación cara a cara entre el equipo, la familia y el niño como una forma de aprehender la situación biográfica y el cuerpo de conocimiento de los sujetos involucrados y luego comprender sus necesidades y demandas reales, promueve la atención integral. A través de la fenomenología social de Alfred Schutz, aporta como implicaciones para la práctica la posibilidad de entender la percepción del equipo de enfermería sobre la relevancia de la relación cara a cara en el cuidado de niños y adolescentes con trastornos mentales.

Abstract Objective to know the perceptions of the nursing team about the care of hospitalized children and adolescents with mental disorders. Method social phenomenological qualitative study whose data collection was carried out with 12 members of the nursing teams by means of phenomenological interviews. Results "Reasons why" described in two categories: the perception of the nursing team in view of the care provided and actions and care of the nursing team of the child with mental disorders. "Reasons for" expressed in the category: the recovery of the child with mental disorder and his return home. Conclusions and Implications for practice The possibility of establishing a face-to-face relationship between the team, the family, and the child to apprehend the biographical situation and the body of knowledge of the subjects involved, and then understand their real needs and demands promotes comprehensive care. Through Alfred Schutz's social phenomenology, it brings as implications for practice the possibility of understanding the perception of the nursing staff about the relevance of the face-to-face relationship in caring for children and adolescents with mental disorders.

Manejo del paciente anticoagulado en una cirugía dental / Management of the anticoagulated patient in dental surgery / Gerenciamento do paciente anticoagulado em cirurgia odontológica

Introducción: una adecuada hemostasia es crucial para el éxito del tratamiento odontológico invasivo, ya que los problemas de sangrado pueden dar lugar a complicaciones asociadas a una importante morbimortalidad. El tratamiento odontológico de pacientes que tienden a un mayor riesgo de sangrado debido al uso de fármacos anticoagulantes plantea un desafío en la práctica diaria de los profesionales de la odontología. El conocimiento adecuado de los mecanismos subyacentes a la hemostasia y el manejo optimizado de estos pacientes son, por lo tanto, cuestiones muy importantes. Se realiza un estudio de los fármacos anticoagulantes actualmente disponibles en el mercado, evaluando los riesgos y beneficios de suspender dicho fármaco previo a un tratamiento odontológico invasivo. Además, se hace una revisión de los protocolos de manejo actuales que se utilizan en estos pacientes. Material y métodos: se realizó una búsqueda bibliográfica en las bases de datos Epistemonikos y Medline/PubMed; en el portal Timbó y en la biblioteca virtual Scielo. Abarcando todos los estudios publicados en los últimos 15 años en inglés y español. Se encontraron 30 artículos, se seleccionaron 15 en primera instancia para finalizar con 11 artículos. En dicha selección el filtro fue que los demás artículos se referían a otros anticoagulantes que no eran parte de este trabajo. Resultados: se han desarrollado múltiples protocolos de manejo, aunque en todos los casos se requiere una historia clínica completa, junto con pruebas hemostáticas complementarias para minimizar los riesgos derivados del tratamiento odontológico. Discusión: muchos autores consideran que la medicación de los pacientes indicada para el tratamiento de una enfermedad de base no debe ser alterada o suspendida a menos que así lo indique el médico prescriptor. Se ha demostrado que las medidas hemostáticas locales son suficientes para controlar los posibles problemas de sangrado derivados del tratamiento dental.

Introduction: Adequate hemostasis is crucial for the success of invasive dental treatment, since bleeding problems can lead to complications associated with significant morbidity and mortality. The dental treatment of patients who are prone to an increased risk of bleeding due to the use of anticoagulant drugs poses a challenge in the daily practice of dental professionals. Adequate knowledge of the mechanisms underlying hemostasis and optimized management of these patients are therefore very important issues. A review is made of the anticoagulant drugs currently available on the market, evaluating the risks and benefits of suspending such a drug prior to invasive dental treatment. In addition, a review is made of the current management protocols used in these patients. Material and methods: A bibliographic search was carried out in the Epistemonikos and Medline/PubMed databases; in the Timbo portal and in the Scielo virtual library. All the studies published in the last 15 years in English and Spanish were included. Thirty articles were found, 15 were selected in the first instance to end up with 11 articles. In this selection, the filter was that the other articles referred to other anticoagulants that were not part of this work. Results: multiple management protocols have been developed, although in all cases a complete clinical history is required, together with complementary hemostatic tests to minimize the risks derived from dental treatment. Discussion: many authors consider that the patient's medication indicated for the treatment of an underlying disease should not be altered or suspended unless so indicated by the prescribing physician. It has been shown that local hemostatic measures are sufficient to control possible bleeding problems derived from dental treatment.

Introdução: A hemostasia adequada é crucial para o sucesso do tratamento dentário invasivo, pois problemas de sangramento podem levar a complicações associadas a uma morbidade e mortalidade significativas. O tratamento odontológico de pacientes que são propensos a um risco maior de sangramento devido ao uso de drogas anticoagulantes representa um desafio na prática diária dos profissionais da odontologia. O conhecimento adequado dos mecanismos subjacentes à hemostasia e o gerenciamento otimizado desses pacientes são, portanto, questões muito importantes. É realizada uma revisão dos anticoagulantes atualmente disponíveis no mercado, avaliando os riscos e benefícios de descontinuar tal medicamento antes do tratamento dentário invasivo. Além disso, é feita uma revisão dos protocolos de gerenciamento atuais usados nesses pacientes. Material e métodos: Foi realizada uma pesquisa bibliográfica nas bases de dados Epistemonikos e Medline/PubMed; no portal Timbo e na biblioteca virtual Scielo. Todos os estudos publicados nos últimos 15 anos, em inglês e espanhol, foram incluídos. Trinta artigos foram encontrados, 15 foram selecionados em primeira instância para acabar com 11 artigos. Nesta seleção, o filtro foi que os outros artigos se referiam a outros anticoagulantes que não faziam parte deste trabalho. Resultados: foram desenvolvidos múltiplos protocolos de gerenciamento, embora em todos os casos seja necessário um histórico clínico completo, juntamente com testes hemostáticos complementares para minimizar os riscos derivados do tratamento odontológico. Discussão: muitos autores consideram que a medicação os pacientes indicada para o tratamento de uma doença subjacente não deve ser alterada ou descontinuada, a menos que o médico que a prescreve dê instruções nesse sentido. Medidas hemostáticas locais demonstraram ser suficientes para controlar potenciais problemas de sangramento resultantes do tratamento odontológico.

Separation and analysis of Bacillus subtilis respiratory chain complexes.

Bacillus subtilis is a Gram-positive bacterium with a respiratory chain embedded in the cytoplasmic membrane. The respiratory chain is bifurcated after menaquinol into a cytochrome b6c + caa3 branch and a branch with up to three quinol oxidases. The complexes that generate the proton gradient are b6c, associated with caa3 and aa3 oxidase. The b6c and caa3 complexes form a supercomplex, and it is proposed to form respiratory strings in the membrane. There is still information missing about the quinol branch and if the primary oxidase quinol aa3 is associated with the electron donor complexes. It is unclear whether succinate quinone reductase (SQR) can form associations with the quinol branch or the cytochrome branch. In this paper, we show the separation of an almost pure b6c complex associated with cytochromes c550 and c551. We obtained a b6c + caa3 supercomplex of 600 kDa and SQR, aa3, and NADH dehydrogenase by dodecyl maltoside solubilization and separation of the respiratory chain components by ionic exchange chromatography. We found that aa3 does not associate with other complexes. SQR was associated with the b6c complex in a mutant lacking aa3. This association could facilitate electron transfer from SQR to menaquinone-7. The lack of associations between the abundant quinol oxidase aa3 and other complexes is a feature we cannot explain yet.

Muscle wasting assessment tools for prostate cancer.

Prostate cancer and its treatment may induce muscle wasting. Body composition and muscle functionality are rarely assessed in patients with prostate cancer from developing countries due to the limited availability of high-quality equipment for routine diagnosis. This cross-sectional study evaluated the association between several simplistic techniques for assessing muscle mass and function with a more complex standard of reference for muscle wasting among Mexican men with prostate cancer. Muscle wasting was highly prevalent, yet it was presumably associated with aging rather than cancer and its treatment itself. The restricted availability of specific equipment in clinical settings with technological limitations supports using unsophisticated techniques as surrogate measurements for muscle wasting. The left-arm handgrip dynamometry displayed the highest correlation with the standard of reference and exhibited an acceptable predicted probability for muscle estimation. Combining several simplistic techniques may be preferable. We also developed and internally validated a manageable model that helps to identify elderly patients with prostate cancer at risk of muscle depletion and impairment. These findings promote the early recognition and treatment of muscle wasting alterations occurring among older adults with prostate cancer.

Solid Carcinoma of the Canine Mammary Gland: a Histological Type or Tumour Cell Arrangement?

Mammary neoplasms are the most frequently diagnosed tumours in female dogs and are classified into various histological types, including solid carcinomas. We proposed a subclassification of solid carcinomas based on morphological and immunohistochemical characteristics, and correlated the subtypes with prognostic factors. A total of 135 cases of solid mammary carcinoma were selected from 3,400 canine mammary neoplasms in the archives of the Laboratory of Comparative Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Brazil. Epidemiological and survival data were obtained, and immunolabelling for chromogranin A, pancytokeratin, cytokeratin 14, Ki67 and p63 was performed. Solid carcinomas were classified into six subgroups: malignant adenomyoepithelioma (68/135), carcinoma with solid pattern (22/135), malignant myoepithelioma (16/135), basaloid carcinoma (14/135), neuroendocrine carcinoma (10/135) and solid papillary carcinoma (5/135). Shorter survival time was associated with the presence of lymphatic invasion (P = 0.009) in the initial clinical staging (I-III). When considering all clinical stages (I-V), vascular invasion (P <0.001) and the presence of regional metastasis (P = 0.004) were important prognostic factors. Basaloid carcinoma and solid papillary carcinoma did not reach the median survival time for early-stage cases, and malignant myoepithelioma had the highest median survival in advanced stages. Carcinoma with a solid pattern was associated with a higher number of regional metastases. Distinguishing the various histological and immunophenotypic subtypes that exhibit a solid arrangement, using histological and immunohistochemical criteria, is essential for understanding the behaviour of these neoplasms and for the selection of more appropriate and specific therapies.

Histopathologic and immunophenotypic profile of a spontaneous mammary carcinoma in a male Humboldt's white-fronted capuchin (Cebus albifrons).

A 20-year-old male captive Humboldt's white-fronted capuchin (Cebus albifrons) that died accidentally had a small non-ulcerative mammary nodule diagnosed as a mammary carcinoma. Immunohistochemistry demonstrated that neoplastic cells were positive for pan-cytokeratin and cytokeratin-7. Approximately 20% of neoplastic cells were positive for Ki-67. Neoplastic cells expressed estrogen and progesterone receptors.

The act of playing as a signifier for the application of the dramatic Therapeutic Toy performed by the nurse: theoretical reflection / El jugar como significante para aplicación del juguete terapéutico dramático por el enfermero: reflexión teórica / O brincar como significante para aplicação do Brinquedo Terapêutico dramático pelo enfermeiro: reflexão teórica

ABSTRACT Objective: To reflect on the possible contributions of the act of playing as a signifier in the use of dramatic Therapeutic Toy performed by the nurse. Methods: Theoretical-reflexive study, based on three moments: The language of the unconscious that emerges as a signifier when playing; How the dramatic Therapeutic Toy is used by the nurse; and the act of playing as a signifier during the application of the dramatic Therapeutic Toy: contributions to nursing practice. Results: Through the intervention of the nurse in the sessions, the act of playing mediated by the dramatic Therapeutic Toy provides the child with the opportunity to elaborate on signifier elements that had not been meant by him. Final considerations: Through the dramatic Therapeutic Toy, the articulation of the child's organism with his reality in the formation of the self constitutes an advance for the production of knowledge and nursing assistance to the child, enabling complete care and allowing the elaboration of their anxieties, which collaborates so that the child constitutes himself as a subject.

RESUMEN Objetivo: Reflejar posibles contribuciones del jugar mientras significante en la utilización del Juguete Terapéutico dramático en la actuación del enfermero. Métodos: Estudio teórico-reflexivo, ancorado en tres momentos: Lenguaje del inconsciente que emerge en el jugar como significante; Como el Juguete Terapéutico dramático es utilizado por el enfermero; y El jugar mientras significante en transcurso de la aplicación del Juguete Terapéutico dramático: contribuciones a práctica de enfermería. Resultados: Por intermedio del manejo de sesiones por el enfermero, el jugar mediado por Juguete Terapéutico dramático proporciona al niño la oportunidad de elaboración de elementos significantes que no le habían sido significados. Consideraciones finales: Mediante Juguete Terapéutico dramático, la articulación del organismo del niño con su realidad en su formación del yo constituye avanzo para producción de conocimiento y asistencia de enfermería pediátrica, posibilitando cuidado integral y permitiendo la elaboración de sus angustias, que colabora para que el niño se constituya mientras sujeto.

RESUMO Objetivo: Refletir sobre possíveis contribuições do brincar enquanto significante na utilização do Brinquedo Terapêutico dramático na atuação do enfermeiro. Métodos: Estudo teórico-reflexivo, ancorado em três momentos: A linguagem do inconsciente que emerge no brincar como significante; Como o Brinquedo Terapêutico dramático é utilizado pelo enfermeiro; e O brincar enquanto significante no decorrer da aplicação do Brinquedo Terapêutico dramático: contribuições para a prática de enfermagem. Resultados: Por intermédio do manejo das sessões pelo enfermeiro, o brincar mediado pelo Brinquedo Terapêutico dramático proporciona à criança a oportunidade de elaboração de elementos significantes que não haviam sido significados por ela. Considerações finais: Mediante o Brinquedo Terapêutico dramático, a articulação do organismo da criança com sua realidade na formação do eu constitui avanço para produção de conhecimento e assistência de enfermagem à criança, possibilitando cuidado integral e permitindo a elaboração de suas angústias, o que colabora para que a criança se constitua enquanto sujeito.

Visita à criança hospitalizada em terapia intensiva: vivências de irmãos reveladas por meio do brinquedo terapêutico dramático / Visita al niño hospitalizado en terapia intensiva: vivencias de hermanos revelado por el juego terapéutico dramático / Visiting a hospitalized child in intensive care: experiences of siblings revealed through the dramatic therapeutic play

Resumo Objetivo: compreender, por meio do brinquedo terapêutico dramático, o significado, para o irmão, de visitar a criança hospitalizada em terapia intensiva. Método: pesquisa qualitativa, modalidade fenomenológica, que utilizou o brinquedo terapêutico dramático para acessar às experiências dos irmãos. Foi realizada em Unidade de Terapia Intensiva Pediátrica do interior do estado de São Paulo, Brasil. Participaram das sessões de brinquedo terapêutico 11 irmãos menores de 10 anos, as quais foram analisadas à luz da Teoria do Amadurecimento. Resultados: os irmãos, tendo um lugar para brincar, dramatizaram situações, anteriormente, vividas, de seu cotidiano e da visita à criança hospitalizada. Ao viver, criativamente, revelaram que brincar é fazer a integração das experiências do "eu", favorecendo o continuar a ser diante da situação vivida. Conclusões e implicações para a prática: o Brinquedo Terapêutico Dramático compreendido à luz de um referencial teórico possibilitou que o irmão significasse a visita como uma experiência de integração do "eu", revelando emoções, desejos e preferências do cotidiano. Nesse sentido, o cuidado ao irmão da criança hospitalizada define-se pela oferta do brincar livre, para que ele demonstre o sentimento de continuar a ser em suas interações com o mundo, no qual o contexto hospitalar tornou parte da realidade.

Resumen Objeto: Comprender por medio del juego terapéutico dramático el significado, para el hermano, de la visita al niño hospitalizado en Terapia Intensiva Pediátrica. Método: Investigación cualitativa, modalidad fenomenológica, que utilizó el juego terapéutico dramático para comprender la experiencia del hermano. Se realizó en Unidad de Terapia Intensiva Pediátrica del interior del Estado de São Paulo, Brasil. Participaron de las sesiones de juego terapéutico 11 hermanos con menos de 10 años, quienes fueron analizados a la luz de la Teoría de la Maduración. Resultados: Los hermanos, al tener un lugar para jugar, dramatizaron situaciones anteriormente vividas, de su cotidiano y de la visita al niño hospitalizado. Al vivir de forma creativa, revelaron que jugar es permitir la integración de las experiencias del "yo", lo que favorece el concepto de seguir siendo, ante la situación vivida. Conclusiones e implicaciones para la práctica: El Juego Terapéutico Dramático comprendido a la luz de un referencial teórico hizo posible que el hermano entendiera la visita como una experiencia de integración del "yo", revelando emociones, deseos y preferencias cotidianas. En este sentido, el cuidado del hermano del niño hospitalizado se define por la oferta de juego libre, para que pueda demostrar su sentimiento de seguir siendo en sus interacciones con el mundo, en el que el contexto hospitalario se ha convertido en parte de la realidad.

Abstract Objective: to understand, by means of dramatic therapeutic play, the meaning, for the sibling, of visiting the child hospitalized in intensive care. Method: a qualitative research, phenomenological modality, which used the dramatic therapeutic play to access the siblings' experiences. It was carried out in a Pediatric Intensive Care Unit in the countryside of the State of São Paulo, Brazil. Eleven siblings under ten years of age participated in the therapeutic play sessions, which were analyzed in the light of the Theory of Maturation. Results: the siblings, having a place to play, dramatized previously lived situations, from their daily life and from the visit to the hospitalized child. By living creatively, they revealed that playing is to integrate the experiences of the "I", favoring the continuity of being in the face of the situation lived. Conclusions and implications for practice: the Dramatic Therapeutic Play understood in the light of a theoretical framework allowed the sibling to mean the visit as an experience of integration of the "I", revealing emotions, desires and preferences of daily life. In this sense, the care for the brother of the hospitalized child is defined by the offer of free play, so that he demonstrates the feeling of continuing to be in his interactions with the world, in which the hospital context has become part of reality.

The act of playing as a signifier for the application of the dramatic Therapeutic Toy performed by the nurse: theoretical reflection.

OBJECTIVE: To reflect on the possible contributions of the act of playing as a signifier in the use of dramatic Therapeutic Toy performed by the nurse. METHODS: Theoretical-reflexive study, based on three moments: The language of the unconscious that emerges as a signifier when playing; How the dramatic Therapeutic Toy is used by the nurse; and the act of playing as a signifier during the application of the dramatic Therapeutic Toy: contributions to nursing practice. RESULTS: Through the intervention of the nurse in the sessions, the act of playing mediated by the dramatic Therapeutic Toy provides the child with the opportunity to elaborate on signifier elements that had not been meant by him. FINAL CONSIDERATIONS: Through the dramatic Therapeutic Toy, the articulation of the child's organism with his reality in the formation of the self constitutes an advance for the production of knowledge and nursing assistance to the child, enabling complete care and allowing the elaboration of their anxieties, which collaborates so that the child constitutes himself as a subject.