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J Hazard Mater ; 470: 134271, 2024 May 15.
Artículo en Inglés | MEDLINE | ID: mdl-38608593

RESUMEN

Rapid and sensitive monitoring of pH and histamine is crucial for bridging biological and food systems and identifying corresponding abnormal situations. Herein, N-doped carbon dots (CDs) are fabricated by a hydrothermal method employing dipicolinic acid and o-phenylenediamine as precursors. The CDs exhibit colorimetric and fluorescent dual-mode responses to track pH and histamine variations in living cells and food freshness, respectively. The aggregation-induced emission enhancement and intramolecular charge transfer result in a decrease in absorbance and an increase in fluorescence, which become readily apparent as the pH changes from acidic to neutral. This property enables precise differentiation between normal and cancerous cells. Furthermore, given the intrinsic basicity of histamine, pH-responsive CDs are advantageous for additional colorimetric and fluorescent monitoring of histamine in food freshness, achieving linearities of 25-1000 µM and 30-1000 µM, respectively, which are broader than those of alternative nanoprobes. Interestingly, the smartphone-integrated sensing platform can portably and visually evaluate pH and histamine changes due to sensitive color changes. Therefore, the sensor not only establishes a dynamic connection between pH and histamine for the purposes of biological and food monitoring, but also presents a novel approach for developing a multifunctional biosensor that can accomplish environmental monitoring and biosensing simultaneously.


Asunto(s)
Carbono , Colorimetría , Histamina , Puntos Cuánticos , Histamina/análisis , Carbono/química , Colorimetría/métodos , Concentración de Iones de Hidrógeno , Puntos Cuánticos/química , Humanos , Técnicas Biosensibles/métodos , Espectrometría de Fluorescencia , Teléfono Inteligente , Análisis de los Alimentos/métodos , Nitrógeno/química , Fluorescencia , Colorantes Fluorescentes/química
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