RESUMEN
Non-A, non-B viral hepatitis was transmitted to four colony-born chimpanzees by infusion of three lots of antihemophilic factor (factor VIII) implicated in the transmission of non-A, non-B hepatitis to two human recipients. All four inoculated animals showed histopathological evidence of viral hepatitis, and all demonstrated significant ALT elevations between seven and one-half weeks after inoculation. Acute-phase plasma from one of the infected chimpanzees (no. 771) was shown to induce non-A, non-B hepatitis in two other chimpanzees approximately three weeks after their inoculation. In addition, an acute-phase open liver wedge biopsy obtained from animal no. 771 was processed and examined by immune electron microscopy (IEM) for virus-like particles with convalescent serum from a serologically confirmed case of non-A, non-B hepatitis. Twenty-five to 30 nm (mean = 27 nm) diameter virus-like particles that were either "full" or "empty" were identified in this liver preparation by IEM. Two additional chimpanzees inoculated with a cesium chloride gradient fraction of an isopycnically banded liver homogenate (animal no. 771) also developed elevated ALT activity two to two and one-half weeks later. Our findings have experimentally verified that commercially produced factor VIII materials can induce non-A, non-B hepatitis in champanzees and that the disease can be subpassaged in these animals by inoculation of either acute-phase plasma or liver. These results also provide evidence for the association of 27 nm-diameter virus-like particles with non-A, non-B viral hepatitis.
Asunto(s)
Factor VIII/efectos adversos , Virus de Hepatitis/aislamiento & purificación , Adulto , Alanina Transaminasa/sangre , Animales , Femenino , Virus de Hepatitis/ultraestructura , Hepatitis Viral Humana/enzimología , Hepatitis Viral Humana/microbiología , Hepatitis Viral Humana/transmisión , Humanos , Hígado/microbiología , Masculino , Pan troglodytesAsunto(s)
Marcapaso Artificial/efectos adversos , Anciano , Femenino , Humanos , Masculino , Persona de Mediana EdadAsunto(s)
Educación Médica , Hospitales de Veteranos , Facultades de Medicina , Hospitales de Enseñanza , Humanos , KansasRESUMEN
This report describes a relatively asymptomatic 52-year-old man who had a markedly enlarged cardiac silhouette on chest roentgenogram when first seen. Clinical studies confirmed the presence of a mass, and thoracotomy revealed a huge intrathoracic tumor. The lesion was interpreted as an intrapulmonary hamartoma that had undergone malignant degeneration.