RESUMEN
OBJECTIVE: To compare open pulled straws with conventional sorans system for the purpose of establishing an effective vitrification method for mouse blastocysts. METHODS: With open pulled straws (OPS) and conventional straws, vitrifi- cation of mouse blastocysts was performed, and the survival and hatching rates of the blastocysts were observed after thawing. RESULTS: After vitrification, the embryo survival in the OPS group (82.3%) was significantly higher than conventional straw group (68.6%, P<0.01), and the hatching rate was also higher (70.3%, P<5 CONCLUSIONS: Vitrification of mouse blastocysts using OPS results in higher survival and hatching rates as compared with conventional straw method.
Asunto(s)
Blastocisto/fisiología , Criopreservación/métodos , Transferencia de Embrión , Animales , Femenino , Ratones , EmbarazoRESUMEN
OBJECTIVE: To investigate whether phorbol 12-myristate 13-acetate (PMA) modifies the invasive ability of trophoblast cells by regulating their cytokine productions. METHODS: Reverse transcriptase-polymerase chain reaction was used to examine the effect of PMA on the expression of cytokines which regulated the invasive ability of trophoblast cells. RESULTS: Prior to PMA treatment, expressions of the cytokins including hepatocyte growth factor (HGF), interleukin (IL)-1beta, insulin-like growth factor (IGF)-II, transforming growth factor (TGF)- beta and vascular endothelial growth factor (VEGF) were all detected in JAR cells, only with the exception of IGF-I. After incubation with 100 nmol/L PMA for 24 h, the cells showed strong expression of IL-1beta, HGF and IGF-II, with reduced expression of TGF-beta2 and TGF-beta3. CONCLUSION: By regulating the autocrine of these cytokines, PMA exercises its effect to enhance the invasive ability of trophoblast or choriocarcinoma cells.
Asunto(s)
Coriocarcinoma/patología , Citocinas/genética , Acetato de Tetradecanoilforbol/farmacología , Trofoblastos/efectos de los fármacos , Neoplasias Uterinas/patología , Línea Celular , Factores de Crecimiento Endotelial/genética , Femenino , Humanos , Péptidos y Proteínas de Señalización Intercelular/genética , Linfocinas/genética , Invasividad Neoplásica , Embarazo , ARN Mensajero/análisis , Somatomedinas/genética , Factor de Crecimiento Transformador beta/genética , Trofoblastos/inmunología , Trofoblastos/patología , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial VascularRESUMEN
OBJECTIVE: To explore the relevance of the embryo morphology with the clinical result of intracytoplasmic sperm injection (ISCI) for infertility treatment. METHODS: A retrospective analysis was conducted of 291 ISCI and 352 in vitro fertility (IVF) cycles collected during the period from Juanary, 1999 to March, 2001. RESULTS: Significant difference was not found in the fertility rates between the 2 groups (75.7% vs 73.2%, P=0.5738), nor in the oocyte lysis rates (10.6% vs 7.9%, P=0.238). The rates of the embryos with more than 20% fragments were similar between the 2 groups (41.2% vs 55.8%, P=0.137), but ICSI group had a significantly lower rate of blastocyst formation (43.1% vs 55.8%, P<0.001). The clinical pregnancy rates and miscarriage rates did not vary significantly between the 2 groups (34.7% vs 39.9%, P=0.189; 8.9% vs 13.4%, P=0.079). CONCLUSIONS: ISCI is an effective treatment for male infertility to achieve uneventful clinical pregnancy.