Ultrasonic seed treatment improved seed germination, growth, and yield of rice by modulating associated physio-biochemical mechanisms.

Ultrasonic seed (US) treatment could alter seed germination mechanism, however, US induced alterations in morph-physiological attributes and yield of fragrant rice were rarely reported. In the present study, the seeds of three fragrant rice cultivars viz., Xiangyaxiangzhan, Meixiangzhan 2, Ruanhuayou 6100 and one non-fragrant rice viz., Wufengyou 615 were exposed to ultrasonic waves at 20-40 kHz for 1.5 min (T) whereas the seeds without exposure were taken as control (CK). Results showed that US treatment caused minor cracks on seed surface while improved seed germination rate (1.79 %-11.09 %) and 3-indoleacetic acid (IAA) (3.36 %-46.91 %). Furthermore, peroxidase (POD) activity and methionine sulfoxide reductase activity was increased by 29.15 %-74.13 % and 11.26 %-20.87 %, respectively; however, methionine sulfoxide reductase related protein repairing gene MSRA4 was down-regulated by 17.93 % -41.04 % under T, compared to CK. Besides, US treatment also improved soluble protein in flag leaf (0.92 %-40.79 %), photosynthesis (3.37 %-16.46 %), biomass (5.17 %-31.87 %), as well as 2-acetyl-1-pyrroline content (4.77 %-15.48 %) in rice grains. In addition, multivariate analysis showed that the dry weight at the maturity stage were significantly related to the POD, glutathione reductase (GR) activity, IAA, and abscisic acid (ABA) content while germination rate was positively related to the GR activity, ABA content, and yield, but which were negatively related to the IAA and gibberellic acid content.

Silica nanoparticles conferring resistance to bacterial wilt in peanut (Arachis hypogaea L.).

Peanut bacterial wilt (PBW) caused by the pathogen Ralstonia solanacearum severely affects the growth and yield potential of peanut crop. In this study, we synthesized silica nanoparticles (SiO2 NPs), a prospective efficient management approach to control PBW, and conducted a hydroponic experiment to investigate the effects of different SiO2 NPs treatments (i.e., 0, 100, and 500 mg L-1 as NP0, NP100, and NP500, respectively) on promoting plant growth and resistance to R. solanacearum. Results indicated that the disease indices of NP100 and NP500 decreased by 51.5 % and 55.4 % as compared with NP0 under R. solanacearum inoculation, respectively, while the fresh and dry weights and shoot length of NP100 and NP500 increased by 7.62-42.05 %, 9.45-32.06 %, and 2.37-17.83 %, respectively. Furthermore, SiO2 NPs induced an improvement in physio-biochemical enzymes (superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, and lipoxygenase) which eliminated the excess production of hydrogen peroxide, superoxide anions, and malondialdehyde to alleviate PBW stress. Notably, the targeted metabolomic analysis indicated that SiO2 NPs enhanced salicylic acid (SA) contents, which involved the induction of systemic acquired resistance (SAR). Moreover, the transcriptomic analysis revealed that SiO2 NPs modulated the expression of multiple transcription factors (TFs) involved in the hormone pathway, such as AHLs, and the identification of hormone pathways related to plant defense responses, such as the SA pathway, which activated SA-dependent defense mechanisms. Meanwhile, the up-regulated expression of the SA-metabolism gene, salicylate carboxymethyltransferase (SAMT), initiated SAR to promote PBW resistance. Overall, our findings revealed that SiO2 NPs, functioning as a plant elicitor, could effectively modulate physiological enzyme activities and enhance SA contents through the regulation of SA-metabolism genes to confer the PBW resistance in peanuts, which highlighted the potential of SiO2 NPs for sustainable agricultural practices.

l-Glutamate Seed Priming Enhances 2-Acetyl-1-pyrroline Formation in Fragrant Rice Seedlings in Response to Arsenite Stress.

2-Acetyl-1-pyrroline (2-AP) is a fragrance compound and flavor in fragrant rice whose precursors are generally glutamate (Glu) and proline (Pro). Our previous study revealed that exogenous Glu enhanced the arsenic (As) tolerance in fragrant rice by improving the ascorbic acid-glutathione cycle and the Pro content in roots. However, less is known about how Glu is involved in 2-AP biosynthesis in fragrant rice under As stress. Herein, a hydroponic experiment of L-Glu seed priming with 0, 100, and 500 µM l-glutamic acid solutions was conducted with two fragrant rice varieties. After that, the 10-day-old seedlings were cultured under 0 and 100 µM arsenite stress for 10 d. Results showed that the 2-AP and Pro contents were increased by 18-30% and 21-78% under As100 µM-Glu100 µM treatment in comparison to the control As100 µM to Glu0 µM, while the activities of pyrroline-5-carboxylate synthetase (P5CS) and proline dehydrogenase (ProDH) were increased by 19-46% and 3-19%, respectively. Furthermore, the 2-AP, Pro contents, and P5CS activity were correlated positively. Correspondingly, a significant abundance of differential expressed metabolites (18) and differential expressed genes (26) was observed in amino acid metabolism and glutathione metabolism pathways. In addition, several essential genes were verified and grouped into the pathways of glutathione metabolism, proline, and arginine metabolism with antioxidant defense system to comodulate 2-AP biosynthesis and stress detoxification. Therefore, the Glu seed priming treatment had a positive impact on the 2-AP biosynthesis of fragrant rice under 100 µM arsenite toxicity.

Application of glutamic acid improved As tolerance in aromatic rice at early growth stage.

To alleviate the arsenic (As) toxicity in aromatic rice, a hydroponic experiment of two As concentrations (0 and 100 µM sodium arsenite: A0, A1), three glutamic acid (Glu) concentrations (0, 100, and 500 µM l-glutamic acid: G0, G1, and G2) with Xiangyaxiangzhan and Meixiangzhan 2 was conducted. Results showed that the root As content were increased under A1G2 but reduced under A1G1 for Xiangyaxiangzhan as compared with A1G0. A decrement of As was transported from root to shoot caused by up-regulated OsABCC1 relative expression in Meixiangzhan 2. Likewise, As stress enhanced the H2O2 and malondialdehyde content, resulting in the impaired cell wall observed by transmission electron microscopy. However, compared with A1G0, the superoxide dismutase activity, ascorbic acid, glutathione, proline, and soluble sugar content were increased under A1G1. Additionally, arsenate reductase, monodehydroascorbate reductase activity, Glu, proline, and soluble sugar content were found positively associated with the As accumulation. Further, the metabolome analysis indicated that the pathway of amino acid and arginine biosynthesis were notably enriched after Glu application. Generally, 100 µM Glu application was the better treatment to enhance As tolerance in aromatic rice through up-regulating amino acid biosynthesis with increasing antioxidants and osmolytes to scavenge excessive reactive oxygen species.

Insights of Improved Aroma under Additional Nitrogen Application at Booting Stage in Fragrant Rice.

Plant mineral nutrition substantially affects the growth, yield and quality of rice, whereas nitrogen (N) application contributes significantly in this regard. Undoubtedly, N application improves rice aroma biosynthesis; however, the molecular mechanism underlying the regulation of grain 2-acetyl-1-pyrroline (2-AP) biosynthesis in the presence of nitrogen application at the booting stage has remained largely unexplored. The present study examined the effects of three N levels, i.e., 0 g per pot (N0), 0.43 g per pot (N1) and 0.86 g per pot (N2) on intermediates, enzymes and genes involved in 2-AP biosynthesis, as well as on the yield of two fragrant rice cultivars viz, Meixiangzhan2 and Xiangyaxiangzhan. N was additionally applied at the booting stage. The results depicted that the levels of precursor, such as proline, and the activity of enzymes involved in 2-AP biosynthesis, such as Δ1-pyrroline-5-carboxylate synthetase (P5CS) and diamine oxidase (DAO), and P5CS1 gene expression were comparatively higher under N1 than N0 in both fragrant rice cultivars. Moreover, the N2 treatment increased the grain panicle-1, filled grain percentage and grain yield of both rice cultivars, while the grain yield of Meixiangzhan2 and Xiangyaxiangzhan was increased by 15.87% and 12.09%, respectively, under N2 compared to N1 treatment. Hence, 0.43 g per pot of N showed positive performances in yield and aroma accumulation in fragrant rice and should be further employed in the practice and production for better cultivation in the rice market.

Effect of Exogenous Melatonin Application on the Grain Yield and Antioxidant Capacity in Aromatic Rice under Combined Lead-Cadmium Stress.

This study aimed to determine the mechanism of exogenous melatonin application in alleviating the combined Pb and Cd (Pb-Cd) toxicity on aromatic rice (Oryza sativa L.). In this study, a pot experiment was conducted; two aromatic rice varieties, Yuxiangyouzhan and Xiangyaxiangzhan, were selected, and sprays using 50, 100, 200, and 400 µmol L-1 melatonin (denoted as S50, S100, S200, and S400) and irrigation using 100, 300, and 500 µmol L-1 melatonin (denoted as R100, R300, and R500) were also selected. The results showed that, under the S50, S100, and S200 treatments, the Pb content of aromatic rice grain decreased, and the grain yield increased significantly. Moreover, the application of exogenous melatonin significantly reduced the accumulation of H2O2 in rice leaves at maturity under Cd-Pb stress and reduced the MDA content in Xiangyaxiangzhan leaves. In addition, the microbial community structure changed significantly under S50 and R300 treatments. Some pathways, such as the synthesis of various amino acids and alanine, aspartate, and glutamate metabolism, were regulated by S50 treatment. Overall, melatonin application improved aromatic rice grain yield while reducing heavy metal accumulation by regulating the antioxidant capacity and metabolites in aromatic rice plants and altering the physicochemical properties and microbial community structures of the soil.

Silicon Application Modulates the Growth, Rhizosphere Soil Characteristics, and Bacterial Community Structure in Sugarcane.

Silicon (Si) deficiency, caused by acidic soil and rainy climate, is a major constraint for sugarcane production in southern China. Si application generally improves sugarcane growth; however, there are few studies on the relationships between enhanced plant growth, changes in rhizosphere soil, and bacterial communities. A field experiment was conducted to measure sugarcane agronomic traits, plant nutrient contents, rhizosphere soil enzyme activities and chemical properties, and the rhizosphere bacterial community diversity and structure of three predominant sugarcane varieties under two Si treatments, i.e., 0 and 200 kg of silicon dioxide (SiO2) ha-1 regarded as Si0 and Si200, respectively. Results showed that Si application substantially improved the sugarcane stalk fresh weight and Si, phosphorus (P), and potassium (K) contents comparing to Si0, and had an obvious impact on rhizosphere soil pH, available Si (ASi), available P (AP), available K (AK), total phosphorus (TP), and the activity of acid phosphatase. Furthermore, the relative abundances of Proteobacteria showed a remarkable increase in Si200, which may be the dominant group in sugarcane growth under Si application. Interestingly, the AP was noticed as a major factor that caused bacterial community structure differences between the two Si treatments according to canonical correspondence analysis (CCA). In addition, the association network analysis indicated that Si application enriched the rhizosphere bacterial network, which could be beneficial to sugarcane growth. Overall, appropriate Si application, i.e., 200 kg SiO2 ha-1 promoted sugarcane growth, changed rhizosphere soil enzyme activities and chemical properties, and bacterial community structures.

Ultrasonic seed treatment improved morpho-physiological and yield traits and reduced grain Cd concentrations in rice.

Rice cultivation under cadmium (Cd) contaminated soil often results in reduced growth with excess grain Cd concentrations. A pot experiment was conducted to assess the potential of ultrasonic seed treatment to alleviate Cd stress in rice. Seeds of two aromatic rice cultivars i.e., Xiangyaxiangzhan and Meixiangzhan 2 and two non-aromatic rice cultivars i.e., Huahang 31 and Guangyan 1 were exposed to ultrasonic waves for 1.5 min in 20-40 KHz mixing frequency. The experimental treatments were comprised of untreated seeds (U0) and ultrasonic treated seeds (U1) transplanted in un-contaminated soil (H0) and Cd-contaminated soil (H1). Results revealed that Cd contents and Cd accumulation in grain in U1 were 33.33-42.31% and 12.86-57.58% lower than U0 for fragrant rice cultivars under H1. Meanwhile, biomass production was higher in U1 than U0 under H0 and better yield was assessed in U1 for all cultivars under H1. The activity of peroxidase (POD) in flag leaves was increased by 8.28-115.65% for all cultivars while malondialdehyde (MDA) contents were significantly decreased in U1 compared with U0 under H0. Conclusively, ultrasonic treatment modulated Cd distribution and accumulation in different parts while improved physiological performance as well as yield and grain quality of rice under Cd contaminated conditions.

Transcriptome Analysis Revealed the Mechanisms Involved in Ultrasonic Seed Treatment-Induced Aluminum Tolerance in Peanut.

Ultrasonic (US) treatment is an efficient method to induce crop tolerance against heavy metal toxicity; however, US-induced aluminum (Al) tolerance in peanuts was rarely studied. This study was comprised of two treatments, namely, CK, without ultrasonic treatment, and US, an ultrasonic seed treatment, for 15 min. Both treated and non-treated treatments were applied with Al in the form of AlCl3.18H2O at 5 mmol L-1 in Hoagland solution at one leaf stage. Results depicted that plant height, main root length, and number of lateral roots increased significantly under US treatment. Transcriptome analysis revealed that plant hormone signal transduction and transcription factors (TFs) were significantly enriched in the differentially expressed genes (DEGs) in US treatment, and the plant hormones were measured, including salicylic acid (SA) and abscisic acid (ABA) contents, were substantially increased, while indole acetic acid (IAA) and jasmonic acid (JA) contents were decreased significantly in US treatment. The TFs were verified using quantitative real-time (qRT)-PCR, and it was found that multiple TFs genes were significantly upregulated in US treatment, and ALMT9 and FRDL1 genes were also significantly upregulated in US treatment. Overall, the US treatment induced the regulation of hormone content and regulated gene expression by regulating TFs to improve Al tolerance in peanuts. This study provided a theoretical rationale for US treatment to improve Al tolerance in peanuts.

Application of inorganic passivators reduced Cd contents in brown rice in oilseed rape-rice rotation under Cd contaminated soil.

Immobilization of heavy metals by the application of chemical amendments is an eco-friendly, economical and effective method to remediate cadmium (Cd) -contaminated soils. Field experiments were conducted during 2016-2018 following oilseed rape-rice rotation with the application of inorganic passivators i.e., slaked lime (CaOH2>95%) and sepiolite (SiO2>50%, MgO>20%). The experimental treatments were comprised of: rice planting in winter fallow and without passivator (F-PA0); fallow with slaked lime (F-PA1); fallow with sepiolite (F-PA2); oilseed rape-rice rotation without passivator (R-PA0); rotation with slaked lime (R-PA1); and rotation with sepiolite (R-PA2). The slaked lime and sepiolite were applied after the harvest of rape at 2000 and 5000 kg ha-1, respectively. Results revealed that the Cd contents were reduced by 47.44-49.03% in brown rice for F-PA2 and 9.54-42.66% in soil for R-PA2. The superoxide dismutase (SOD) activity was decreased by 10.65-17.98%, the malondialdehyde (MDA) contents were reduced by 28.57% whereas the proline content was decreased by 32.61% under R-PA2. In addition, the filled grain percentage was improved by 6.87% (F-PA2) and 3.70% (R-PA1), respectively. Overall, rice sown after oilseed rape gave better yields than sown after fallow fields while application of slaked lime and sepiolite as passivator could be a potential management option to grow crops in metal polluted soils.

Ultrasonic seed treatment improved cadmium (Cd) tolerance in Brassica napus L.

Cadmium (Cd) affects crop growth and productivity by disrupting normal plant metabolism. To determinate whether ultrasonic (US) seed treatment can alleviate Cd stress in rape (Brassica napus L.), the seeds of two oilseed rape cultivars i.e., 'Youyanzao18' and 'Zaoshu104' were exposed to ultrasonic waves for 1 min at 20 KHz frequency. Seeds without US treatment were taken as control (CK). Results revealed that the germination rate of both cultivars was significantly (P < 0.05) higher in US treatment than CK only at 0 and 10 mg Cd L-1. The shoot and root length of both cultivars were significantly higher in US treatment than CK at all Cd treatments except the root length of Youyanzao18 at 50 mg Cd L-1. The fresh weight Youyanzao18 was significantly (P < 0.05) higher in US than CK except for Youyanzao18 at 25 mg Cd L-1. Moreover, the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) activities and the proline, glutathione (GSH), and soluble protein contents in Youyanzao18 were relatively higher in the US treatment than CK. The malondialdehyde (MDA) contents were prominently reduced in US treatment than CK. The pods per plant, seeds per pod and rapeseed yield were increased by 15.9, 11.4, and 16.4% in Youyanzao18 and 10.3, 9.5, and 11.5% in Zaoshu104, respectively in US treatment, compared to CK. Moreover, the contents of Cd in root, stem, leaf, rape pod shell, and rapeseeds were comparatively less in US treatment than CK whereas the Cd concentrations in different plant parts of both rape cultivars were recorded as: leaf ˃ root ˃ stem ˃ rape pod shell ˃ rapeseed. In sum, the US treatment improved the morphological growth and rapeseed yield whereas reduced the Cd accumulation in different plant parts of rapeseed under Cd contaminated soil.

Ultrasonic seed treatment improved physiological and yield traits of rice under lead toxicity.

Lead (Pb) is a highly toxic metal that damages normal plant metabolism and often results in reduced crop growth and yield. To measure whether or not ultrasonic seed (US) treatment alleviates Pb stress in rice, the seeds of two rice cultivars, i.e., Guangyan1 and Huahang31, were exposed to 30 min of ultrasonic vibration and then the seedlings were transplanted to Pb-contaminated soil. Results revealed that the Pb contents in roots, stems, leaves, panicles (at heading), and brown rice (at maturity) were lower in US treatment than control (CK; without US treatment); the trend for accumulation of Pb in different plant parts was recorded as: root ˃ stems ˃ leaves ˃ panicles ˃ brown rice in both rice cultivars. Overall, the Pb contents in the brown rice of Huahang31 were higher than those in Guangyan1. Moreover, the contents of proline and soluble protein as well as the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) were significantly higher while the malondialdehyde (MDA) contents were significantly reduced in US treatment compared to CK. The effective panicle number, seed set percentage, grains per panicle, 1000-grain weight, and grain yield were increased by 8.9%, 5.3%, 6.8%, 4.4%, and 26.6% in Guangyan1 and 3.2%, 5.7%, 5.7%, 5.0%, and 24.2% in Huahang31, respectively in US treatment compared to CK. Hence, seed treatment with ultrasonic waves could improve rice performance and reduce brown rice Pb accumulation under Pb-polluted soils.

Mesenchymal stem cells enhanced cardiac nerve sprouting via nerve growth factor in a rat model of myocardial infarction.

BACKGROUND: Transplantation of mesenchymal stem cells (MSCs) alters the ventricular electrophysiologic properties after myocardial infarction (MI) in rats. However, it is unclear whether MSCs transplantation enhances the secretion of nerve growth factor (NGF) and affects cardiac sympathetic remodeling. METHODS: MI was induced in 35 male Sprague-Dawley rats. Two weeks later, the animals were randomized to MSCs or phosphate buffer solution (PBS) injections into the infarcted myocardium. Six weeks thereafter, the expressions of NGF, growth associated protein 43 (GAP43) and tyrosine hydroxylase (TH) were measured and the density of GAP43 and TH positive nerves was calculated in the borderzone. NGF levels were detected in different culture conditions with neonatal rat ventricular myocytes (NRVMs, 2 × 10(5)/well) and MSCs (2 × 10(5)/well). RESULTS: Compared with PBS, mRNA expression and protein levels of NGF, GAP43 and TH increased in the border zone after MSCs injection. Immunohistochemistry showed more GAP43- and TH-positive nerves in the MSCs than in the PBS group. Compared to monocultured MSCs, mono-cultured NRVMs secreted more NGF in vitro. CONCLUSIONS: The expression of NGF increased after MSCs transplantation, which may affect sympathetic remodeling and the electrophysiological properties after MI. Paracrine factors secreted by MSC-CM may be involved in this process.