Velocity-Load Jump Testing Predicts Acceleration Performance in Elite Speed Skaters: But Does Movement Specificity Matter?

PURPOSE: In this study, we compared the influence of movement specificity during velocity-load jump testing to predict on-ice acceleration performance in elite speed skaters. METHODS: Elite long-track speed skaters (N = 27) performed velocity-load testing with 3 external loads during unilateral horizontal jumping, lateral jumping, and bilateral vertical countermovement jumping. For the unilateral tests, external load conditions were set to 10 N, 7.5% and 15% of external load relative to body weight. For the countermovement jumping, load conditions were body weight and 30% and 60% of external load relative to body weight. On-ice performance measures were obtained during maximal 50-m accelerations from a standing start, including maximal skating speed, maximal acceleration capacity, and maximum horizontal power. The 100-m split time from a 500-m race was also obtained. Regularized regression models were used to identify the most important predictors of on-ice acceleration performance. In addition to regularized regression coefficients, Pearson correlation coefficients (r) were calculated for all variables retained by the model to assess interrelationships between single predictors and on-ice performance measures. RESULTS: The countermovement jump with 30% of body mass demonstrated the strongest association with maximal skating speed, maximum horizontal power, and 100-m time (regularized regression coefficient = .16-.49, r = .84-.97, P < .001). Horizontal jump with 15% of body mass was the strongest predictor of maximal acceleration capacity performance (regularized regression coefficient = .08, r = .83, P < .001). CONCLUSIONS: The findings of this study suggest that mechanical specificity rather than movement specificity was more relevant for predicting on-ice acceleration performance.

Relationship Between External Training Load and Session Rating of Perceived Exertion Training Impulse in Elite Sprinters.

PURPOSE: To quantify the change in session rating of perceived exertion training impulse (RPE-TRIMP) that may occur in response to increased running distance at 3 running velocity ranges in elite sprinters. METHODS: We monitored training load in elite sprinters (women: n = 7; men: n = 11) using wearable Global Positioning System technology and RPE-TRIMP for a total of 681 individual training sessions during a 22-week competition-preparation period. Internal training load was operationalized by RPE-TRIMP, and external training load was operationalized by distance covered in 3 velocity ranges. A linear mixed-effects model with athlete as a random effect was fit to RPE-TRIMP with total distance covered at ≤69.99% (low-velocity running [LVR]), 70% to 84.99% (high-velocity running [HVR]), and 85% to 100% (very-high-velocity running [VHVR]) of individual maximum velocity. RESULTS: Increased running distance in all 3 velocity ranges (LVR, HVR, and VHVR) resulted in a significant (P < .001) increase in RPE-TRIMP. Coefficients (95% CIs) were .10 (.08-.11) for LVR, .23 (.18-.28) for HVR, and .44 (.35-.53) for VHVR. A 50-m increase in running distance covered in the LVR, HVR, and VHVR velocity ranges was associated with increases in RPE-TRIMP of 5, 11.5, and 22 arbitrary units, respectively. CONCLUSIONS: Internal training load, calculated as RPE-TRIMP, increased with increases in total distance covered in the LVR, HVR, and VHVR velocity ranges (P < .001). RPE-TRIMP can be a practical solution for monitoring global training-session load in elite sprinters.

A Biopsychosocial Model for Understanding Training Load, Fatigue, and Musculoskeletal Sport Injury in University Athletes: A Scoping Review.

ABSTRACT: McClean, ZJ, Pasanen, K, Lun, V, Charest, J, Herzog, W, Werthner, P, Black, A, Vleuten, RV, Lacoste, E, and Jordan, MJ. A biopsychosocial model for understanding training load, fatigue, and musculoskeletal sport injury in university athletes: A scoping review. J Strength Cond Res 38(6): 1177-1188, 2024-The impact of musculoskeletal (MSK) injury on athlete health and performance has been studied extensively in youth sport and elite sport. Current research examining the relationship between training load, injury, and fatigue in university athletes is sparse. Furthermore, a range of contextual factors that influence the training load-fatigue-injury relationship exist, necessitating an integrative biopsychosocial model to address primary and secondary injury prevention research. The objectives of this review were (a) to review the scientific literature examining the relationship between training load, fatigue, and MSK injury in university athletes and (b) to use this review in conjunction with a transdisciplinary research team to identify biopsychosocial factors that influence MSK injury and develop an updated, holistic biopsychosocial model to inform injury prevention research and practice in university sport. Ten articles were identified for inclusion in this review. Key findings were an absence of injury surveillance methodology and contextual factors that can influence the training load-fatigue-MSK injury relationship. We highlight the inclusion of academic load, social load, and mental health load as key variables contributing to a multifactorial, gendered environmental, scientific inquiry on sport injury and reinjury in university sport. An integrative biopsychosocial model for MSK injury in university sport is presented that can be used to study the biological, psychological, and social factors that modulate injury and reinjury risk in university athletes. Finally, we provide an example of how causal inference can be used to maximize the utility of longitudinally collected observational data that is characteristic of sport performance research in university sport.

Longitudinal Monitoring of Biomechanical and Psychological State in Collegiate Female Basketball Athletes Using Principal Component Analysis.

Background: The growth in participation in collegiate athletics has been accompanied by increased sport-related injuries. The complex and multifactorial nature of sports injuries highlights the importance of monitoring athletes prospectively using a novel and integrated biopsychosocial approach, as opposed to contemporary practices that silo these facets of health. Methods: Data collected over two competitive basketball seasons were used in a principal component analysis (PCA) model with the following objectives: (i) investigate whether biomechanical PCs (i.e., on-court and countermovement jump (CMJ) metrics) were correlated with psychological state across a season and (ii) explore whether subject-specific significant fluctuations could be detected using minimum detectable change statistics. Weekly CMJ (force plates) and on-court data (inertial measurement units), as well as psychological state (questionnaire) data, were collected on the female collegiate basketball team for two seasons. Results: While some relationships (n = 2) were identified between biomechanical PCs and psychological state metrics, the magnitude of these associations was weak (r = |0.18-0.19|, p < 0.05), and no other overarching associations were identified at the group level. However, post-hoc case study analysis showed subject-specific relationships that highlight the potential utility of red-flagging meaningful fluctuations from normative biomechanical and psychological patterns. Conclusion: Overall, this work demonstrates the potential of advanced analytical modeling to characterize components of and detect statistically and clinically relevant fluctuations in student-athlete performance, health, and well-being and the need for more tailored and athlete-centered monitoring practices.

Kinesiophobia in Injured Athletes: A Systematic Review.

Athletes have a high risk of injury. Kinesiophobia is a condition in which an individual experiences a fear of physical movement and activity after an injury occurs. Our purpose was to systematically review the literature about Kinesiophobia in athletes. A systematic review was conducted in February 2023 using PubMed, CINAHL, SPORTDiscus, Web of Science, Cochrane Library, and Medline. Studies were included if they were peer-reviewed, in English, within the last 20 years and included athletes who had been injured and tracked Kinesiophobia. Articles were checked for quality via the modified Downs and Black checklist. Fourteen studies were included in the review and had an average "fair" quality score. Authors examined Kinesiophobia in injured athletes with mostly lower-extremity injuries. Kinesiophobia was associated with lower physical and mental outcomes. Kinesiophobia exists in athletes and can affect both physical and mental factors. The Tampa Scale of Kinesiophobia (TSK) was the most common tool used to examine Kinesiophobia. Common mental factors associated with Kinesiophobia include anxiety, low confidence, and fear avoidance.

Inversion for Thermal Properties with Frequency Domain Thermoreflectance.

3D integration of multiple microelectronic devices improves size, weight, and power while increasing the number of interconnections between components. One integration method involves the use of metal bump bonds to connect devices and components on a common interposer platform. Significant variations in the coefficient of thermal expansion in such systems lead to stresses that can cause thermomechanical and electrical failures. More advanced characterization and failure analysis techniques are necessary to assess the bond quality between components. Frequency domain thermoreflectance (FDTR) is a nondestructive, noncontact testing method used to determine thermal properties in a sample by fitting the phase lag between an applied heat flux and the surface temperature response. The typical use of FDTR data involves fitting for thermal properties in geometries with a high degree of symmetry. In this work, finite element method simulations are performed using high performance computing codes to facilitate the modeling of samples with arbitrary geometric complexity. A gradient-based optimization technique is also presented to determine unknown thermal properties in a discretized domain. Using experimental FDTR data from a GaN-diamond sample, thermal conductivity is then determined in an unknown layer to provide a spatial map of bond quality at various points in the sample.

Characterizing empathy and compassion using computational linguistic analysis.

Many scholars have proposed that feeling what we believe others are feeling-often known as "empathy"-is essential for other-regarding sentiments and plays an important role in our moral lives. Caring for and about others (without necessarily sharing their feelings)-often known as "compassion"-is also frequently discussed as a relevant force for prosocial motivation and action. Here, we explore the relationship between empathy and compassion using the methods of computational linguistics. Analyses of 2,356,916 Facebook posts suggest that individuals (N = 2,781) high in empathy use different language than those high in compassion, after accounting for shared variance between these constructs. Empathic people, controlling for compassion, often use self-focused language and write about negative feelings, social isolation, and feeling overwhelmed. Compassionate people, controlling for empathy, often use other-focused language and write about positive feelings and social connections. In addition, high empathy without compassion is related to negative health outcomes, while high compassion without empathy is related to positive health outcomes, positive lifestyle choices, and charitable giving. Such findings favor an approach to moral motivation that is grounded in compassion rather than empathy. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Modeling the Early and Late Acceleration Phases of the Sprint Start in Elite Long Track Speed Skaters.

ABSTRACT: Zukowski, MH, Jordan, MJ, and Herzog, W. Modeling the early and late cceleration phases of the sprint start in elite long track speed skaters. J Strength Cond Res 38(2): 236-244, 2024-This study established the reliability of an exponential function to model the change in velocity during the speed skating sprint start and the validity of associated model parameters in a group of subelite and elite long track speed skaters. Long track speed skaters ( n = 38) performed maximal effort 50-m on-ice accelerations from a standing start while tethered to a horizontal robotic resistance device that sampled position and time data continuously. An exponential function was applied to the raw data to model the change in velocity throughout the acceleration phase and compute the maximal skating speed (MSS), maximal acceleration capacity (MAC), maximum relative net horizontal power ( PMax ), and an acceleration-time constant ( τ ). All constructed models provided a sufficient fit of the raw data ( R -squared > 0.95, mean bias <2%). Intraday reliability of all model parameters ranged from good to excellent (intraclass correlation coefficient >0.8 and coefficient of variation <5%). Strong negative correlations ( r : -0.72 to -0.96) were observed between MSS and PMax and the 10 and 20 m split times measured with the robotic resistance and with 100 split times obtained from 500 m races. Moderate-to-large between-group differences were observed in MSS, MAC, and PMax between the elite vs. subelite speed skaters (Cohen d effect sizes: 1.18-3.53). Our results indicate that monoexponential modeling is a valid and reliable method of monitoring initial acceleration performance in elite level long track speed skaters.

Differences in Situational Patterns During Change of Direction Movements Greater than 90° in Youth Male and Female Soccer Players.

Change of direction (COD) maneuvers in soccer create tactical advantages, but also expose the player to an increased risk of injury. COD ability is commonly tested with pre-planned drills including cuts greater than 90°. These tests do not take into consideration positional differences players encounter during games. This case-series study used principal component analysis (PCA) to examine situational differences during COD movements between playing positions in youth soccer games. For each of the four teams included (26 females, 27 males), one game was analyzed using video-analysis. Two independent reviewers identified situational patterns and a PCA was used to examine differences between playing positions. Three principal components explained 89% of the variation in the data and were categorized as the total quantity of CODs, attacking/goal-scoring and defensive reacting types of CODs. One-way ANOVA on the individual principal component (PC) scores showed significant differences (p < 0.05) between centre midfielders, goalkeepers, and centrebacks in the quantity of CODs (PC1), and between wingers and fullbacks and centre backs in attacking/goal-scoring CODs (PC2), whereas PC3 was not different between playing positions. Differences between playing positions suggest that training and testing protocols in soccer could be enhanced to better match the individual and playing position-based needs.

Validity of Global Positioning System Technology to Measure Maximum Velocity Sprinting in Elite Sprinters.

ABSTRACT: Thome, M, Thorpe, RT, Jordan, MJ, and Nimphius, S. Validity of global positioning system (GPS) technology to measure maximum velocity sprinting in elite sprinters. J Strength Cond Res 37(12): 2438-2442, 2023-The objective of this study was to assess the concurrent validity of 10-Hz wearable Global Positioning System (GPS) technology to measure maximum velocity sprinting (Vmax) relative to Doppler radar in elite sprinters. Data were collected from a single training session performed by elite 100 and 200 m sprinters (males: n = 5; 100 m best times: 10.02 ± 0.07 seconds, range: 9.94-10.10 seconds; 200 m best times: 20.29 ± 0.42 seconds, range: 19.85-20.80 seconds; females: n = 2; age: 28.0 ± 4.2 years; body mass: 65.8 ± 4.6 kg; 100 m best times: 11.18 ± 0.34 seconds; 200 m best times: 22.53 ± 0.04 seconds). Velocity and time data from 16 maximal, 60-m sprint efforts were recorded simultaneously with 10 Hz GPS and 47 Hz radar. Validity was assessed using Bland-Altman 95% limits of agreement (LOA) and intraclass correlation coefficient (ICC), each with respective 95% confidence intervals (CI). Vmax measured with 10 Hz GPS demonstrated a LOA of -0.11 m·s-1 (-0.17, -0.05) and an ICC of 0.99 (0.98, 1.0) relative to the radar device.10 Hz GPS overestimated Vmax by 0.11 m·s-1 relative to the radar but could still be considered a suitable tool for monitoring external load in elite sprinters. However, the much smaller average annual improvement in this population (∼0.1-0.2%) in comparison with the ∼1% overestimation reduces the utility of 10 Hz GPS to detect meaningful performance changes in maximum velocity.

Call for the application of a biopsychosocial and interdisciplinary approach to the return-to-sport framework of snow sports athletes.

Snow sports such as alpine skiing or snowboarding are associated with a high risk of injury and reinjury and are subject to a very special environment with specific rehabilitation challenges that must be addressed. Due to geographic decentralisation, seasonal climatic limitations, alternation of training in off-snow and on-snow settings and unique loading patterns of practising these sports, special rehabilitation structures and processes are required compared with other sports. In addition, returning to preinjury performance requires a high level of confidence and a resumption of risk-taking in demanding situations such as high-speed skiing and high-amplitude jumps. A biopsychosocial and interdisciplinary approach can be viewed as a holistic, athlete-centred approach that promotes interprofessional communication and collaboration. This is particularly central for managing the physical/biological, psychological and social demands of injury management for snow sports. It can help ensure that rehabilitation content is well coordinated and tailored to individual needs. This is because transitions between different rehabilitation phases and caring professionals are well aligned, and rehabilitation is understood not only as purely 'physical recovery' but also as 'psychological recovery' considering the snow sports-specific setting with specific social norms. Ultimately, this may improve the rehabilitation success of snow sports athletes.

Monitoring lower limb biomechanical asymmetry and psychological measures in athletic populations-A scoping review.

BACKGROUND: Lower limb biomechanics, including asymmetry, are frequently monitored to determine sport performance level and injury risk. However, contributing factors extend beyond biomechanical and asymmetry measures to include psychological, sociological, and environmental factors. Unfortunately, inadequate research has been conducted using holistic biopsychosocial models to characterize sport performance and injury risk. Therefore, this scoping review summarized the research landscape of studies concurrently assessing measures of lower limb biomechanics, asymmetry, and introspective psychological state (e.g., pain, fatigue, perceived exertion, stress, etc.) in healthy, competitive athletes. METHODS: A systematic search of MEDLINE, Embase, CINAHL, SPORTDiscus, and Web of Science Core Collections was designed and conducted in accordance with PRISMA guidelines. Fifty-one articles were included in this review. RESULTS: Significant relationships between biomechanics (k = 22 studies) or asymmetry (k = 20 studies) and introspective state were found. Increased self-reported pain was associated with decreased range of motion, strength, and increased lower limb asymmetry. Higher ratings of perceived exertion were related to increased lower limb asymmetry, self-reported muscle soreness, and worse jump performance. Few studies (k = 4) monitored athletes longitudinally throughout one or more competitive season(s). CONCLUSION: This review highlights the need for concurrent analysis of introspective, psychological state, and biomechanical asymmetry measures along with longitudinal research to understand the contributing factors to sport performance and injury risk from biopsychosocial modeling. In doing so, this framework of biopsychosocial preventive and prognostic patient-centered practices may provide an actionable means of optimizing health, well-being, and sport performance in competitive athletes.

Single Leg Lateral and Horizontal Loaded Jump Testing: Reliability and Correlation With Long Track Sprint Speed Skating Performance.

ABSTRACT: Zukowski, MH, Jordan, MJ, and Herzog, W. Single leg lateral and horizontal loaded jump testing: reliability and correlation with long track sprint speed skating performance. J Strength Cond Res 37(11): 2251-2259, 2023-This study examined the intraday reliability of 2 novel unilateral loaded jump protocols designed for long track speed skaters. Highly trained ( n = 26), national level athletes performed single leg jumps with a horizontal robotic resistance across 3 external load conditions (10 N, 7.5% of body mass and 15% of body mass) using their dominant limb. Jumps were performed in both the horizontal (Jump Horz ) and lateral (Jump Lat ) direction to replicate the body position and line of force application observed during the running and gliding phases of on-ice acceleration. Subjects completed 2 consecutive trials of the same jump protocol to examine the intraday reliability of the peak velocity achieved for each loading condition. Peak velocity across each jump type and loading condition had good reliability (intraclass correlation coefficient >0.8, coefficient of variation <5%). Significant positive relationships ( r = 0.5-0.8, p < 0.05; n = 22) were observed between all jump conditions and on-ice sprint race split times obtained including 100, 400, and 500 m. Our results indicate that unilateral loaded jump tests are reliable in speed skating athletes and may help practitioners diagnose and monitor lower-limb maximal muscle power capacity in a sport-specific manner.

Annexin A6 mediates calcium-dependent exosome secretion during plasma membrane repair.

Exosomes are an extracellular vesicle (EV) subtype that is secreted upon the fusion of multivesicular bodies (MVBs) with the plasma membrane. Exosomes may participate in intercellular communication and have utility as disease biomarkers; however, little is known regarding the physiological stimuli that induce their secretion. Ca2+ influx promotes exosome secretion, raising the possibility that exosomes are secreted during the Ca2+-dependent plasma membrane repair of tissues damaged by mechanical stress in vivo. To determine whether exosomes are secreted upon plasma membrane damage, we developed sensitive assays to measure exosome secretion in intact and permeabilized cells. Our results suggest that exosome secretion is coupled to Ca2+-dependent plasma membrane repair. We find that annexin A6 (ANXA6), a well-known plasma membrane repair protein, is recruited to MVBs in the presence of Ca2+ and required for Ca2+-dependent exosome secretion, both in intact and in permeabilized cells. ANXA6 depletion stalls MVBs at the cell periphery, and ANXA6 truncations localize to different membranes, suggesting that ANXA6 may serve to tether MVBs to the plasma membrane. We find that cells secrete exosomes and other EVs upon plasma membrane damage and propose that repair-induced secretion may contribute to the pool of EVs present within biological fluids.

Mechanisms of Anterior Cruciate Ligament Tears in Professional National Basketball Association Players: A Video Analysis.

A systematic search was performed of online databases for any anterior cruciate ligament (ACL) injuries within the NBA. Video was obtained of injuries occurring during competition and downloaded for 2-dimensional video analysis. Thirty-five in-game videos were obtained for analysis. Of the reviewed cases, 19% were noncontact ACL injuries where there was no player-to-player contact from an opposing player. Three injury mechanism categories were found based on the events at the point of initial ground contact of the foot of the injured limb: single-leg casting (mean dorsiflexion angle 18.9° (14.4°); mean knee flexion angle 15.6° (7.8°); and mean trunk lateral flexion 18.2° (8.4°)); bilateral hop (mean dorsiflexion angle 18.2° (15.2°), mean knee flexion angle 21° (14.5°), mean trunk extension angle 6.9° (11.4°), and landing angle from the athlete's center of mass 47.9° (10.1°)); and single-leg landing after contact (mean abduction angle of the swing leg 105.4° (18.1°), mean knee flexion angle of the injured limb 34.2° (8.0°), and mean trunk ipsilateral flexion angle 22.2° (7.0°)).

Mind-bending geometry: Children's and adults' intuitions about linearity on spheres.

Humans appear to intuitively grasp definitions foundational to formal geometry, like definitions that describe points as infinitely small and lines as infinitely long. Nevertheless, previous studies exploring human's intuitive natural geometry have consistently focused on geometric principles in planar Euclidean contexts and thus may not comprehensively characterize humans' capacity for geometric reasoning. The present study explores whether children and adults can reason about linearity in spherical contexts. We showed 48 children (age range: 6-8 years) and 48 adults from the U.S. Northeast two different paths between the same two points on pictures of spheres and asked them to judge which path was the most efficient for an actor to get from a starting point to a goal object. In one kind of trial, both paths looked curved in the pictures, and in another kind of trial, the correct curved-looking path was paired with an incorrect straight-looking path. Adults were successful on both kinds of trials, and although children often chose the incorrect straight-looking path, they were surprisingly successful at identifying the efficient path when comparing two that were curved. Children thus may build on a natural geometry that gives us humans intuitions that are not limited to the formal axioms of Euclidean geometry or even to the Euclidean plane. (PsycInfo Database Record (c) 2023 APA, all rights reserved).

The Effect of Physical Exertional Testing on Postconcussion Symptom Scale Scores in Male and Female High School Students.

PURPOSE: Symptom scores commonly measured following concussion were compared between male and female adolescents with (Hx+) and without (Hx-) a history of concussion, pre and post physical exertion testing. METHODS: Eighty (males [n = 60; Hx+ = 19], female [n = 20; Hx+ = 5]) high school students (ages 15-17 y) completed the Buffalo Concussion Treadmill Test once and the modified shuttle run test twice. Symptom scores were collected using the 22-point Symptom Evaluation Scale on the Sport Concussion Assessment Tool (version 5) immediately pre and post physical exertion testing. RESULTS: The symptoms most reported during preexertional testing were fatigue/low energy, feeling slowed down, and nervous/anxious, whereas feeling slowed down, fatigue/low energy, "pressure in head" (males only), and headache (females only) were most frequently reported during postexertion testing. CONCLUSION: An understanding of the common exertion-related symptoms at baseline in a laboratory or field-based setting in adolescents may be advantageous for clinicians as they manage individual recovery postconcussion. This is particularly important during an adolescent's recovery and return to play when exertional testing may be implemented, especially since symptoms were reported pre and post exertional testing in both males and females regardless of concussion history.

Differences in kinetic variables between injured and uninjured rearfoot runners: A hierarchical cluster analysis.

Running is a popular form of physical activity with a high incidence of running-related injuries. However, the etiology of running-related injuries remains elusive, possibly due to the heterogeneity of movement patterns. The purpose of this study was to investigate whether different clusters existed within a large group of injured and uninjured runners based on their kinetic gait patterns. A sample of 134 injured and uninjured runners were acquired from an existing database and 12 discrete kinetic and spatiotemporal variables which are commonly associated with running injuries were extracted from the ground reaction force waveforms. A principal components analysis followed by an unsupervised hierarchical cluster analysis was performed. The results revealed two distinct clusters of runners which were not associated with injury status (OR = 1.14 [0.57, 2.30], χ2  = 0.143, p = 0.706) or sex (OR = 1.72 [0.85, 3.49], χ2  = 2.3258, p = 0.127). These results suggest that while there appeared to be evidence for two distinct clusters within a large sample of injured and uninjured runners, there is no association between the kinetic variables and running related injuries.

Metal retention and replacement in QueD2 protect queuosine-tRNA biosynthesis in metal-starved Acinetobacter baumannii.

In response to bacterial infection, the vertebrate host employs the metal-sequestering protein calprotectin (CP) to withhold essential transition metals, notably Zn(II), to inhibit bacterial growth. Previous studies of the impact of CP-imposed transition-metal starvation in A. baumannii identified two enzymes in the de novo biosynthesis pathway of queuosine-transfer ribonucleic acid (Q-tRNA) that become cellularly abundant, one of which is QueD2, a 6-carboxy-5,6,7,8-tetrahydropterin (6-CPH4) synthase that catalyzes the initial, committed step of the pathway. Here, we show that CP strongly disrupts Q incorporation into tRNA. As such, we compare the AbQueD2 "low-zinc" paralog with a housekeeping, obligatory Zn(II)-dependent enzyme QueD. The crystallographic structure of Zn(II)-bound AbQueD2 reveals a distinct catalytic site coordination sphere and assembly state relative to QueD and possesses a dynamic loop, immediately adjacent to the catalytic site that coordinates a second Zn(II) in the structure. One of these loop-coordinating residues is an invariant Cys18, that protects QueD2 from dissociation of the catalytic Zn(II) while maintaining flux through the Q-tRNA biosynthesis pathway in cells. We propose a "metal retention" model where Cys18 introduces coordinative plasticity into the catalytic site which slows metal release, while also enhancing the metal promiscuity such that Fe(II) becomes an active cofactor. These studies reveal a complex, multipronged evolutionary adaptation to cellular Zn(II) limitation in a key Zn(II) metalloenzyme in an important human pathogen.

Mechanisms of Achilles Tendon Rupture in National Basketball Association Players.

A systematic search was performed of online databases for any Achilles tendon (AT) injuries occurring within the National Basketball Association (NBA). Video was obtained of injuries occurring during competition and downloaded for analysis in Dartfish. NBA athletes (n = 27) were identified with AT rupture over a 30-year period (1991-2021). Of the 27 NBA athletes found to have AT ruptures (mean age: 29.3 [3.3] y; average time in the NBA: 8.5 [3.8] y), 15 in-game videos were obtained for analysis. Noncontact rupture was presumed to have occurred in 12/13 cases. Eight of the 13 athletes had possession of the ball during time of injury. The ankle joint of the injured limb for all 13 athletes was in a dorsiflexed position during the time of injury (47.9° [6.5°]). All 13 athletes performed a false-step mechanism at time of injury where they initiated the movement by taking a rearward step posterior to their center of mass with the injured limb before translating forward. NBA basketball players that suffered AT ruptures appeared to present with a distinct sequence of events, including initiating a false step with ankle dorsiflexion of the injured limb at the time of injury.