Effects of temperature on biological activity of permafrost microorganisms.

The number and viability of microorganism specimens Bacillus spp. isolated from permafrost soil remained unchanged after incubation at temperatures of -16-37°C. Experiments on F1 CBA/Black-6 mice showed that incubation of bacteria at -5°C for 72 h promotes a decrease in their toxicity and an increase in their immunostimulating effect.

Effects of low-doses of Bacillus spp. from permafrost on differentiation of bone marrow cells.

The effects of a new microorganism species (Bacillus spp., strain M3) isolated from permafrost specimens from Central Yakutia (Mamontova Mountain) on the bone marrow hemopoiesis were studied on laboratory mice. Analysis of the count and immunophenotype of bone marrow cells indicated that even in low doses (1000-5000 microbial cells) these microorganisms modulated hemopoiesis and lymphopoiesis activity. The percentage of early hemopoietic precursors (CD117(+)CD34(-)) increased, intensity of lymphocyte precursor proliferation and differentiation (CD25(+)CD44(-)) decreased, and the percentage of lymphocytes released from the bone marrow (CD25(+)CD44(+)) increased on day 21 after injection of the bacteria. These changes in activity of hemopoiesis were associated with changes in the level of regulatory T lymphocytes (reduced expression of TCRαß) and were most likely compensatory. The possibility of modulating hemopoiesis activity in the bone marrow by low doses of one microorganism strain isolated from the permafrost could be useful for evaluating the effects of other low dose bacteria on the bone marrow hemopoiesis.

Effects of short-term local warm and cold applications to the chest on immunophysiological parameters of the organism.

Applications of cold and warm activated cellular immunity and orientation and exploratory behavior in mice. Application of cold increased the number of turns to the left in the open field, muscle force, and activity of the food instinct, and caused an increase in the total count of peripheral blood leukocytes. Warm applications reduced the food instinct activity and stimulated the humoral immunity.