RESUMEN
The fly Drosophila yakuba has lost an ancestral component of the male courtship song: this is due to ontogenetic death of effector neurons in the ventral nerve cord, a result of the D. yakuba sex-determining gene dsx producing a male isoform, dsxM, with cell-death-promoting activity similar to that of the female isoform, dsxF, in D. melanogaster.
Asunto(s)
Cortejo , Proteínas de Drosophila , Drosophila , Conducta Sexual Animal , Animales , Masculino , Conducta Sexual Animal/fisiología , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Femenino , Drosophila/fisiología , Drosophila/genética , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Neuronas/fisiología , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismoRESUMEN
Vocal communication is used across extant vertebrates, is evolutionarily ancient, and been maintained, in many lineages. Here I review the neural circuit architectures that support intraspecific acoustic signaling in representative anuran, mammalian and avian species as well as two invertebrates, fruit flies and Hawaiian crickets. I focus on hindbrain motor control motifs and their ties to respiratory circuits, expression of receptors for gonadal steroids in motor, sensory, and limbic neurons as well as divergent modalities that evoke vocal responses. Hindbrain and limbic participants in acoustic communication are highly conserved, while forebrain participants have diverged between anurans and mammals, as well as songbirds and rodents. I discuss the roles of natural and sexual selection in driving speciation, as well as exaptation of circuit elements with ancestral roles in respiration, for producing sounds and driving rhythmic vocal features. Recent technical advances in whole brain fMRI across species will enable real time imaging of acoustic signaling partners, tying auditory perception to vocal production.
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Acústica , Sonido , Animales , Neuronas , Percepción Auditiva , Rombencéfalo , Drosophila , MamíferosRESUMEN
The sex-determining genes Double Sex and Fruitless are expressed in sexually differentiated neurons of the Drosophila brain. A tiny cluster of neurons, aDN cells, serves as a key circuit switch with sexually dimorphic properties: those of female flies respond to visual signals in males, while those of male flies respond to smell and humidity in females, supporting effective courtship and communal egg laying behaviors, respectively.
Asunto(s)
Proteínas de Drosophila , Drosophila , Animales , Encéfalo/metabolismo , Cortejo , Drosophila/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Femenino , Masculino , Caracteres Sexuales , Factores de Transcripción/metabolismoRESUMEN
In many species, vocal communication is essential for coordinating social behaviors including courtship, mating, parenting, rivalry, and alarm signaling. Effective communication requires accurate production, detection, and classification of signals, as well as selection of socially appropriate responses. Understanding how signals are generated and how acoustic signals are perceived is key to understanding the neurobiology of social behaviors. Here we review our long-standing research program focused on Xenopus, a frog genus which has provided valuable insights into the mechanisms and evolution of vertebrate social behaviors. In Xenopus laevis, vocal signals differ between the sexes, through development, and across the genus, reflecting evolutionary divergence in sensory and motor circuits that can be interrogated mechanistically. Using two ex vivo preparations, the isolated brain and vocal organ, we have identified essential components of the vocal production system: the sexually differentiated larynx at the periphery, and the hindbrain vocal central pattern generator (CPG) centrally, that produce sex- and species-characteristic sound pulse frequencies and temporal patterns, respectively. Within the hindbrain, we have described how intrinsic membrane properties of neurons in the vocal CPG generate species-specific vocal patterns, how vocal nuclei are connected to generate vocal patterns, as well as the roles of neurotransmitters and neuromodulators in activating the circuit. For sensorimotor integration, we identified a key forebrain node that links auditory and vocal production circuits to match socially appropriate vocal responses to acoustic features of male and female calls. The availability of a well supported phylogeny as well as reference genomes from several species now support analysis of the genetic architecture and the evolutionary divergence of neural circuits for vocal communication. Xenopus thus provides a vertebrate model in which to study vocal communication at many levels, from physiology, to behavior, and from development to evolution. As one of the most comprehensively studied phylogenetic groups within vertebrate vocal communication systems, Xenopus provides insights that can inform social communication across phyla.
Asunto(s)
Comunicación Animal , Red Nerviosa/fisiología , Rombencéfalo/fisiología , Vocalización Animal/fisiología , Xenopus laevis/fisiología , Estimulación Acústica , Animales , Cartílago Aritenoides/fisiología , Evolución Biológica , Generadores de Patrones Centrales/fisiología , Femenino , Hormonas Esteroides Gonadales/fisiología , Técnicas In Vitro , Músculos Laríngeos/fisiología , Nervios Laríngeos/fisiología , Masculino , Bulbo Raquídeo/fisiología , Neurotransmisores/fisiología , Caracteres Sexuales , Conducta Sexual Animal/fisiología , Conducta Social , Especificidad de la EspecieRESUMEN
Listeners locate potential mates using species-specific vocal signals. As tetrapods transitioned from water to land, lungs replaced gills, allowing expiration to drive sound production. Some frogs then returned to water. Here we explore how air-driven sound production changed upon re-entry to preserve essential acoustic information on species identity in the secondarily aquatic frog genus Xenopus. We filmed movements of cartilage and muscles during evoked sound production in isolated larynges. Results refute the current theory for Xenopus vocalization, cavitation, and favor instead sound production by mechanical excitation of laryngeal resonance modes following rapid separation of laryngeal arytenoid discs. Resulting frequency resonance modes (dyads) are intrinsic to the larynx rather than due to neuromuscular control. Dyads are a distinctive acoustic signature. While their component frequencies overlap across species, their ratio is shared within each Xenopus clade providing information on species identity that could facilitate both conspecific localization and ancient species divergence. Editorial note: This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).
Asunto(s)
Vocalización Animal/fisiología , Agua , Xenopus/fisiología , Animales , Fenómenos Biomecánicos , Cartílago/fisiología , Laringe/fisiología , Movimiento (Física) , Filogenia , Sonido , Xenopus/anatomía & histologíaRESUMEN
To identify mechanisms of behavioral evolution, we investigated the hindbrain circuit that generates distinct vocal patterns in two closely related frog species. Male Xenopus laevis and Xenopus petersii produce courtship calls that include a fast trill: trains of â¼60 Hz sound pulses. Although fast trill rates are similar, X. laevis fast trills have a longer duration and period than those of X. petersii To pinpoint the neural basis of these differences, we used whole-cell patch-clamp recordings in a key premotor hindbrain nucleus (the Xenopus parabrachial area, PBX) in ex vivo brains that produce fictive vocalizations, vocal nerve activity corresponding to advertisement call patterns. We found two populations of PBX neurons with distinct properties: fast trill neurons (FTNs) and early vocal neurons (EVNs). FTNs, but not EVNs, appear to be intrinsically tuned to produce each species' call patterns because: (1) X. laevis FTNs generate longer and slower depolarizations than X. petersii FTNs during their respective fictive vocalizations, (2) current steps in FTNs induce burst durations that are significantly longer in X. laevis than X. petersii, and (3) synaptically isolated FTNs oscillate in response to NMDA in a species-specific manner: longer and slower in X. laevis than in X. petersii Therefore, divergence of premotor neuron membrane properties is a strong candidate for generating vocal differences between species.SIGNIFICANCE STATEMENT The vertebrate hindbrain includes multiple neural circuits that generate rhythmic behaviors including vocalizations. Male African clawed frogs produce courtship calls that are unique to each species and differ in temporal patterns. Here, we identified two functional subtypes of neurons located in the parabrachial nucleus: a hindbrain region implicated in vocal and respiratory control across vertebrates. One of these neuronal subtypes exhibits distinct properties across species that can account for the evolutionary divergence of song patterns. Our results suggest that changes to this group of neurons during evolution may have had a major role in establishing novel behaviors in closely related species.
Asunto(s)
Evolución Biológica , Neuronas/fisiología , Rombencéfalo/fisiología , Vocalización Animal/fisiología , Animales , Masculino , Especificidad de la Especie , XenopusRESUMEN
The vertebrate hindbrain includes neural circuits that govern essential functions including breathing, blood pressure and heart rate. Hindbrain circuits also participate in generating rhythmic motor patterns for vocalization. In most tetrapods, sound production is powered by expiration and the circuitry underlying vocalization and respiration must be linked. Perception and arousal are also linked; acoustic features of social communication sounds-for example, a baby's cry-can drive autonomic responses. The close links between autonomic functions that are essential for life and vocal expression have been a major in vivo experimental challenge. Xenopus provides an opportunity to address this challenge using an ex vivo preparation: an isolated brain that generates vocal and breathing patterns. The isolated brain allows identification and manipulation of hindbrain vocal circuits as well as their activation by forebrain circuits that receive sensory input, initiate motor patterns and control arousal. Advances in imaging technologies, coupled to the production of Xenopus lines expressing genetically encoded calcium sensors, provide powerful tools for imaging neuronal patterns in the entire fictively behaving brain, a goal of the BRAIN Initiative. Comparisons of neural circuit activity across species (comparative neuromics) with distinctive vocal patterns can identify conserved features, and thereby reveal essential functional components.
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Prosencéfalo/fisiología , Rombencéfalo/fisiología , Vocalización Animal/fisiología , Xenopus laevis/fisiología , Animales , Espiración/fisiología , Técnicas de Cultivo de ÓrganosRESUMEN
The neural circuits underlying divergent courtship behaviors of closely related species provide a framework for insight into the evolution of motor patterns. In frogs, male advertisement calls serve as unique species identifiers and females prefer conspecific to heterospecific calls. Advertisement calls of three relatively recently (â¼8.5â Mya) diverged species - Xenopus laevis, X. petersii and X. victorianus - include rapid trains of sound pulses (fast trills). We show that while fast trills are similar in pulse rate (â¼60 pulses s-1) across the three species, they differ in call duration and period (time from the onset of one call to the onset of the following call). Previous studies of call production in X. laevis used an isolated brain preparation in which the laryngeal nerve produces compound action potentials that correspond to the advertisement call pattern (fictive calling). Here, we show that serotonin evokes fictive calling in X. petersii and X. victorianus as it does in X. laevis As in X. laevis, fictive fast trill in X. petersii and X. victorianus is accompanied by an N-methyl-d-aspartate receptor-dependent local field potential wave in a rostral hindbrain nucleus, DTAM. Across the three species, wave duration and period are strongly correlated with species-specific fast trill duration and period, respectively. When DTAM is isolated from the more rostral forebrain and midbrain and/or more caudal laryngeal motor nucleus, the wave persists at species-typical durations and periods. Thus, intrinsic differences within DTAM could be responsible for the evolutionary divergence of call patterns across these related species.
Asunto(s)
Evolución Biológica , Vocalización Animal , Xenopus/fisiología , Potenciales de Acción , Animales , Femenino , Nervios Laríngeos/fisiología , Masculino , Receptores de N-Metil-D-Aspartato/metabolismo , Rombencéfalo/fisiología , Serotonina/metabolismo , Especificidad de la Especie , Proteínas de Xenopus/metabolismoRESUMEN
Medullary motoneurons drive vocalization in many vertebrate lineages including fish, amphibians, birds, and mammals. The developmental history of vocal motoneuron populations in each of these lineages remains largely unknown. The highly conserved transcription factor Paired-like Homeobox 2b (Phox2b) is presumed to be expressed in all vertebrate hindbrain branchial motoneurons, including laryngeal motoneurons essential for vocalization in humans. We used immunohistochemistry and in situ hybridization to examine Phox2b protein and mRNA expression in caudal hindbrain and rostral spinal cord motoneuron populations in seven species across five chordate classes. Phox2b was present in motoneurons dedicated to sound production in mice and frogs (bullfrog, African clawed frog), but not those in bird (zebra finch) or bony fish (midshipman, channel catfish). Overall, the pattern of caudal medullary motoneuron Phox2b expression was conserved across vertebrates and similar to expression in sea lamprey. These observations suggest that motoneurons dedicated to sound production in vertebrates are not derived from a single developmentally or evolutionarily conserved progenitor pool.
Asunto(s)
Evolución Biológica , Neuronas Motoras/fisiología , Vertebrados/fisiología , Vocalización Animal/fisiología , AnimalesRESUMEN
Mating depends on the accurate detection of signals that convey species identity and reproductive state. In African clawed frogs, Xenopus, this information is conveyed by vocal signals that differ in temporal patterns and spectral features between sexes and across species. We characterized spectral sensitivity using auditory-evoked potentials (AEPs), commonly known as the auditory brainstem response, in males and females of four Xenopus species. In female X. amieti, X. petersii, and X. laevis, peripheral auditory sensitivity to their species own dyad-two, species-specific dominant frequencies in the male advertisement call-is enhanced relative to males. Males were most sensitive to lower frequencies including those in the male-directed release calls. Frequency sensitivity was influenced by endocrine state; ovariectomized females had male-like auditory tuning while dihydrotestosterone-treated, ovariectomized females maintained female-like tuning. Thus, adult, female Xenopus demonstrate an endocrine-dependent sensitivity to the spectral features of conspecific male advertisement calls that could facilitate mating. Xenopus AEPs resemble those of other species in stimulus and level dependence, and in sensitivity to anesthetic (MS222). AEPs were correlated with body size and sex within some species. A frequency following response, probably encoded by the amphibian papilla, might facilitate dyad source localization via interaural time differences.
Asunto(s)
Percepción Auditiva/fisiología , Sistema Endocrino/fisiología , Potenciales Evocados Auditivos del Tronco Encefálico/fisiología , Caracteres Sexuales , Vocalización Animal/fisiología , Xenopus/fisiología , Estimulación Acústica/métodos , Aminobenzoatos/farmacología , Andrógenos/administración & dosificación , Anestésicos/farmacología , Animales , Percepción Auditiva/efectos de los fármacos , Peso Corporal , Dihidrotestosterona/administración & dosificación , Sistema Endocrino/efectos de los fármacos , Potenciales Evocados Auditivos del Tronco Encefálico/efectos de los fármacos , Femenino , Masculino , Ovariectomía , Especificidad de la EspecieRESUMEN
African clawed frogs, genus Xenopus, are extraordinary among vertebrates in the diversity of their polyploid species and the high number of independent polyploidization events that occurred during their diversification. Here we update current understanding of the evolutionary history of this group and describe six new species from west and central sub-Saharan Africa, including four tetraploids and two dodecaploids. We provide information on molecular variation, morphology, karyotypes, vocalizations, and estimated geographic ranges, which support the distinctiveness of these new species. We resurrect Xenopus calcaratus from synonymy of Xenopus tropicalis and refer populations from Bioko Island and coastal Cameroon (near Mt. Cameroon) to this species. To facilitate comparisons to the new species, we also provide comments on the type specimens, morphology, and distributions of X. epitropicalis, X. tropicalis, and X. fraseri. This includes significantly restricted application of the names X. fraseri and X. epitropicalis, the first of which we argue is known definitively only from type specimens and possibly one other specimen. Inferring the evolutionary histories of these new species allows refinement of species groups within Xenopus and leads to our recognition of two subgenera (Xenopus and Silurana) and three species groups within the subgenus Xenopus (amieti, laevis, and muelleri species groups).
Asunto(s)
Poliploidía , Xenopus/anatomía & histología , Xenopus/genética , África Central , África Occidental , Animales , Evolución Biológica , Código de Barras del ADN Taxonómico , ADN Mitocondrial , Haplotipos , Hibridación Genética , Cariotipo , Fenotipo , Filogenia , Filogeografía , Xenopus/clasificaciónRESUMEN
The extant species of African clawed frogs (Xenopus and Silurana) provide an opportunity to link the evolution of vocal characters to changes in the responsible cellular and molecular mechanisms. In this review, we integrate several robust lines of research: evolutionary trajectories of Xenopus vocalizations, cellular and circuit-level mechanisms of vocalization in selected Xenopus model species, and Xenopus evolutionary history and speciation mechanisms. Integrating recent findings allows us to generate and test specific hypotheses about the evolution of Xenopus vocal circuits. We propose that reduced vocal sex differences in some Xenopus species result from species-specific losses of sexually differentiated neural and neuromuscular features. Modification of sex-hormone-regulated developmental mechanisms is a strong candidate mechanism for reduced vocal sex differences.
Asunto(s)
Evolución Biológica , Cortejo , Vocalización Animal/fisiología , Xenopus/fisiología , Potenciales de Acción , Animales , Generadores de Patrones Centrales/fisiología , Femenino , Hormonas Esteroides Gonadales/fisiología , Músculos Laríngeos/fisiología , Músculos Laríngeos/ultraestructura , Laringe/anatomía & histología , Masculino , Neuronas Motoras/fisiología , Fibras Musculares de Contracción Rápida/fisiología , Cadenas Pesadas de Miosina/fisiología , Oscilometría , Filogenia , Ranidae/anatomía & histología , Ranidae/fisiología , Mecánica Respiratoria , Rombencéfalo/anatomía & histología , Rombencéfalo/fisiología , Caracteres Sexuales , Espectrografía del Sonido , Especificidad de la Especie , Xenopus/anatomía & histologíaRESUMEN
Phylogenetic studies can reveal patterns of evolutionary change, including the gain or loss of elaborate courtship traits in males. Male African clawed frogs generally produce complex and rapid courtship vocalizations, whereas female calls are simple and slow. In a few species, however, male vocalizations are also simple and slow, suggesting loss of male-typical traits. Here, we explore features of the male vocal organ that could contribute to loss in two species with simple, slow male calls. In Xenopus boumbaensis, laryngeal morphology is more robust in males than in females. Larynges are larger, have a more complex cartilaginous morphology and contain more muscle fibers. Laryngeal muscle fibers are exclusively fast-twitch in males but are both fast- and slow-twitch in females. The laryngeal electromyogram, a measure of neuromuscular synaptic strength, shows greater potentiation in males than in females. Male-specific physiological features are shared with X. laevis, as well as with a species of the sister clade, Silurana tropicalis, and thus are likely ancestral. In X. borealis, certain aspects of laryngeal morphology and physiology are sexually monomorphic rather than dimorphic. In both sexes, laryngeal muscle fibers are of mixed-twitch type, which limits the production of muscle contractions at rapid intervals. Muscle activity potentiation and discrete tension transients resemble female rather than male X. boumbaensis. The de-masculinization of these laryngeal features suggests an alteration in sensitivity to the gonadal hormones that are known to control the sexual differentiation of the larynx in other Xenopus and Silurana species.
Asunto(s)
Vocalización Animal , Xenopus/fisiología , Animales , Electromiografía , Femenino , Laringe/fisiología , Masculino , Fibras Musculares Esqueléticas/fisiología , Caracteres Sexuales , Especificidad de la EspecieRESUMEN
Work on vocal communication, influenced by a drive to understand the evolution of language, has focused on auditory processing and forebrain control of learned vocalizations. The actual hindbrain neural mechanisms used to create communication signals are understudied, in part because of the difficulty of experimental studies in species that rely on respiration for vocalization. In these experimental systems-including those that embody vocal learning-vocal behaviors have rhythmic qualities. Recent studies using molecular markers and 'fictive' patterns produced by isolated brains are beginning to reveal how hindbrain circuits generate vocal patterns. Insights from central pattern generators for respiration and locomotion are illuminating common neural and developmental mechanisms. Choice of vocal patterns is responsive to socially salient input. Studies of the vertebrate social brain network suggest mechanisms used to integrate socially salient information and produce an appropriate vocal response.
Asunto(s)
Encéfalo/fisiología , Comunicación , Conducta Social , Vocalización Animal/fisiología , Animales , Encéfalo/anatomía & histología , Toma de Decisiones , Humanos , Vías Nerviosas/fisiologíaRESUMEN
Social interaction requires that relevant sensory information is collected, classified, and distributed to the motor areas that initiate an appropriate behavioral response. Vocal exchanges, in particular, depend on linking auditory processing to an appropriate motor expression. Because of its role in integrating sensory information for the purpose of action selection, the amygdala has been implicated in social behavior in many mammalian species. Here, we show that two nuclei of the extended amygdala play essential roles in vocal communication in the African clawed frog, Xenopus laevis. Transport of fluorescent dextran amines identifies the X. laevis central amygdala (CeA) as a target for ascending auditory information from the central thalamic nucleus and as a major afferent to the vocal pattern generator of the hindbrain. In the isolated (ex vivo) brain, electrical stimulation of the CeA, or the neighboring bed nucleus of the stria terminalis (BNST), initiates bouts of fictive calling. In vivo, lesioning the CeA of males disrupts the production of appropriate vocal responses to females and to broadcasts of female calls. Lesioning the BNST in males produces an overall decrease in calling behavior. Together, these results suggest that the anuran CeA evaluates the valence of acoustic cues and initiates socially appropriate vocal responses to communication signals, whereas the BNST plays a role in the initiation of vocalizations.
Asunto(s)
Amígdala del Cerebelo/fisiología , Generadores de Patrones Centrales/fisiología , Conformidad Social , Vocalización Animal/fisiología , Amígdala del Cerebelo/citología , Animales , Vías Auditivas/citología , Vías Auditivas/fisiología , Generadores de Patrones Centrales/citología , Señales (Psicología) , Femenino , Masculino , Xenopus laevisRESUMEN
Independent or convergent evolution can underlie phenotypic similarity of derived behavioural characters. Determining the underlying neural and neuromuscular mechanisms sheds light on how these characters arose. One example of evolutionarily derived characters is a temporally simple advertisement call of male African clawed frogs (Xenopus) that arose at least twice independently from a more complex ancestral pattern. How did simplification occur in the vocal circuit? To distinguish shared from divergent mechanisms, we examined activity from the calling brain and vocal organ (larynx) in two species that independently evolved simplified calls. We find that each species uses distinct neural and neuromuscular strategies to produce the simplified calls. Isolated Xenopus borealis brains produce fictive vocal patterns that match temporal patterns of actual male calls; the larynx converts nerve activity faithfully into muscle contractions and single clicks. In contrast, fictive patterns from isolated Xenopus boumbaensis brains are short bursts of nerve activity; the isolated larynx requires stimulus bursts to produce a single click of sound. Thus, unlike X. borealis, the output of the X. boumbaensis hindbrain vocal pattern generator is an ancestral burst-type pattern, transformed by the larynx into single clicks. Temporally simple advertisement calls in genetically distant species of Xenopus have thus arisen independently via reconfigurations of central and peripheral vocal neuroeffectors.
Asunto(s)
Encéfalo/fisiología , Nervios Laríngeos/fisiología , Vocalización Animal/fisiología , Xenopus/fisiología , Comunicación Animal , Animales , Evolución Biológica , Técnicas In Vitro , Laringe/fisiología , Masculino , Neuronas/fisiología , Rombencéfalo/fisiología , Serotonina/fisiología , Especificidad de la EspecieRESUMEN
The developmental programs that contribute to myogenic stem cell proliferation and muscle fiber differentiation control fiber numbers and twitch type. In this study, we describe the use of an experimental model system-androgen-regulated laryngeal muscle of juvenile clawed frogs, Xenopus laevis-to examine the contribution of proliferation by specific populations of myogenic stem cells to expression of the larynx-specific myosin heavy chain isoform, LM. Androgen treatment of juveniles (Stage PM0) resulted in upregulation of an early (Myf-5) and a late (myogenin) myogenic regulatory factor; the time course of LM upregulation tracked that of myogenin. Myogenic stem cells stimulated to proliferate by androgen include a population that expresses Pax-7, a marker for the satellite cell myogenic stem cell population. Since androgen can switch muscle fiber types from fast to slow even in denervated larynges, we developed an ex vivo culture system to explore the relation between proliferation and LM expression. Cultured whole larynges maintain sensitivity to androgen, increasing in size and LM expression. Blockade of cell proliferation with cis-platin prevents the switch from slow to fast twitch muscle fibers as assayed by ATPase activity. Blockade of cell proliferation in vivo also resulted in inhibition of LM expression. Thus, both in vivo and ex vivo, inhibition of myogenic stem cell proliferation blocks androgen-induced LM expression and fiber type switching in juveniles.
Asunto(s)
Andrógenos/metabolismo , Músculos Laríngeos/crecimiento & desarrollo , Desarrollo de Músculos/fisiología , Cadenas Pesadas de Miosina/metabolismo , Células Madre/fisiología , Andrógenos/farmacología , Animales , Diferenciación Celular/efectos de los fármacos , Diferenciación Celular/fisiología , Dihidrotestosterona/metabolismo , Dihidrotestosterona/farmacología , Inmunohistoquímica , Hibridación in Situ , Músculos Laríngeos/efectos de los fármacos , Microscopía Confocal , Desarrollo de Músculos/efectos de los fármacos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Células Madre/efectos de los fármacos , Xenopus laevisRESUMEN
Perception of the temporal structure of acoustic signals contributes critically to vocal signaling. In the aquatic clawed frog Xenopus laevis, calls differ primarily in the temporal parameter of click rate, which conveys sexual identity and reproductive state. We show here that an ensemble of auditory neurons in the laminar nucleus of the torus semicircularis (TS) of X. laevis specializes in encoding vocalization click rates. We recorded single TS units while pure tones, natural calls, and synthetic clicks were presented directly to the tympanum via a vibration-stimulation probe. Synthesized click rates ranged from 4 to 50 Hz, the rate at which the clicks begin to overlap. Frequency selectivity and temporal processing were characterized using response-intensity curves, temporal-discharge patterns, and autocorrelations of reduplicated responses to click trains. Characteristic frequencies ranged from 140 to 3,250 Hz, with minimum thresholds of -90 dB re 1 mm/s at 500 Hz and -76 dB at 1,100 Hz near the dominant frequency of female clicks. Unlike units in the auditory nerve and dorsal medullary nucleus, most toral units respond selectively to the behaviorally relevant temporal feature of the rate of clicks in calls. The majority of neurons (85%) were selective for click rates, and this selectivity remained unchanged over sound levels 10 to 20 dB above threshold. Selective neurons give phasic, tonic, or adapting responses to tone bursts and click trains. Some algorithms that could compute temporally selective receptive fields are described.
Asunto(s)
Comunicación Animal , Nervio Coclear/fisiología , Mesencéfalo/fisiología , Vocalización Animal/fisiología , Xenopus laevis/fisiología , Estimulación Acústica , Algoritmos , Animales , Fenómenos Electrofisiológicos/fisiología , Femenino , Masculino , Conducta Sexual Animal/fisiologíaRESUMEN
Seasonal courtship signals, such as mating calls, are orchestrated by steroid hormones. Sex differences are also sculpted by hormones, typically during brief sensitive periods. The influential organizational-activational hypothesis [50] established the notion of a strong distinction between long-lasting (developmental) and cyclical (adult) effects. While the dichotomy is not always strict [1], experimental paradigms based on this hypothesis have indeed revealed long-lasting hormone actions during development and more transient anatomical, physiological and behavioral effects of hormonal variation in adulthood. Sites of action during both time periods include forebrain and midbrain sensorimotor integration centers, hindbrain and spinal cord motor centers, and muscles. African clawed frog (Xenopus laevis) courtship vocalizations follow the basic organization-activation pattern of hormone-dependence with some exceptions, including expanded steroid-sensitive periods. Two highly-tractable preparations-the isolated larynx and the fictively calling brain-make this model system powerful for dissecting the hierarchical action of hormones. We discuss steroid effects from larynx to forebrain, and introduce new directions of inquiry for which Xenopus vocalizations are especially well-suited.