RESUMEN
Here we introduce the human induced pluripotent stem cell lines (hiPSCs), HIMRi002-A and HIMRi003-A, generated from cultured dermal fibroblasts of 61-year-old (HIMRi002-A) and 38-year-old (HIMRi003-A) female patients, carrying a known heterozygous pathogenic variant (p.A46T) in the Caveolin 3 (CAV3) gene, via lentiviral expression of OCT4, SOX2, KLF4 and c-MYC. HIMRi002-A and HIMRi003-A display typical embryonic stem cell-like morphology, carry the p.A46T CAV3 gene mutation, express several pluripotent stem cell markers, retain normal karyotype (46, XX) and can differentiate in all three germ layers. We postulate that the HIMRi002-A and HIMRi003-A iPSC lines can be used for the characterization of CAV3-associated pathomechanisms and for developing new therapeutic options.
Asunto(s)
Células Madre Pluripotentes Inducidas , Enfermedades Musculares , Células Madre Pluripotentes , Humanos , Femenino , Persona de Mediana Edad , Células Madre Pluripotentes Inducidas/metabolismo , Factor 4 Similar a Kruppel , Enfermedades Musculares/metabolismo , Enfermedades Musculares/patología , Fibroblastos/metabolismo , Mutación , Diferenciación Celular/genéticaRESUMEN
In the present study, Veronica multifida leaf extract and zinc acetate dihydrate were utilized to synthesize zinc oxide (ZnO) nanoparticles (NPs) eco-friendly and cost-effectively under different physical conditions. Soxhlet extractor was used for the preparation of aqueous plant extract. UV-Vis (ultraviolet-visible) spectrophotometer, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and transmission electron microscope (TEM) were used to characterize the ZnO NPs. UV-Vis spectrophotometer in the range of 200-800 nm was used to get information about the formation of ZnO NPs at different pH and temperatures. FTIR spectrum revealed the presence of functional groups in ZnO NPs. XRD, scanning electron microscope, and TEM analyses confirmed the crystal structure and average size of ZnO NPs. The antimicrobial activities of ZnO NPs were tested on microorganisms, that is, Escherichia coli ATCC 43895, Staphylococcus aureus ATCC 29213, Bacillus subtilis, Bacillus licheniformis, Pseudomonas aeruginosa, and Salmonella typhimurium. Moreover, antibiofilm activity of ZnO NPs was performed against P. aeruginosa and S. aureus ATCC 29213. ZnO NPs have shown effective antimicrobial and antibiofilm activities against tested microorganisms. The results elucidated that eco-friendly and cost-effectively produced ZnO NPs could be used as coating materials and in a wide range of industrial applications, such as pharmaceutical industries and cosmetics.
Asunto(s)
Antibacterianos/farmacología , Biopelículas/efectos de los fármacos , Nanopartículas del Metal/química , Veronica/química , Óxido de Zinc/química , Antibacterianos/química , Antibacterianos/metabolismo , Bacterias/efectos de los fármacos , Fenómenos Fisiológicos Bacterianos , Tecnología Química Verde , Pruebas de Sensibilidad Microbiana , Óxido de Zinc/metabolismoRESUMEN
Hermansky-Pudlak Syndrome (HPS) is a rare autosomal recessive disorder presenting with oculocutaneous albinism, bleeding diathesis and lysosomal accumulation of ceroid lipofuscin which leads to interstitial fibrosis in lung. Pulmonary fibrosis which is usually associated with HPS-1 and HPS-4 subtypes usually manifests in the third/fourth decades of life representing with giant lamellar bodies of alveolar type-II-cells and their apparent degeneration causes restrictive lung disease. Pulmonary manifestation of this syndrome may lead to premature death. Pulmonary Alveolar Proteinosis(PAP) is another rare disease characterized by alveolar deposition of surfactant phospholipids and proteins secondary to defective clearance by alveolar macrophages. PAP may occur as autoimmune diseases and/or secondary to toxic inhalation, systemic infections or hematological disorders. None of the cases were reported secondary to HPS according to the best our knowledge. As well, pulmonary involvement of HPS was never reported as PAP. We report the first case of PAP in a patient with HPS.
Asunto(s)
Síndrome de Hermanski-Pudlak , Proteinosis Alveolar Pulmonar , Ceroide , Humanos , Pulmón/metabolismo , Fibrosis PulmonarRESUMEN
BACKGROUND: The presence of restrictive lung disease has classically required the measure of total lung capacity to document 'true' restriction, which has limited its detection in large population-based studies. METHODS: We used spirometric data to classify people with restricted spirometry (forced expiratory volume in 1 second [FEV(1)]/forced vital capacity ≥ 0.70 and FEV(1) < 80% predicted) in the Burden of Lung Disease (BOLD) Study and determined the relation between this finding and demographic factors and the presence of chronic diseases, including diabetes mellitus, hypertension and cardiovascular disease. RESULTS: Overall, we found that 11.7% of men (546/4664) and 16.4% of women (836/5098) had restricted spirometry. Prevalence varied widely by site, from a low of 4.2% among males in Sydney, Australia, to a high of 48.7% among females in Manila, The Philippines. Compared to people with normal lung function, those with restricted spirometry had a higher prevalence of diabetes (12.2% vs. 4.6%), heart disease (15.0% vs. 7.7%) and hypertension (38.8% vs. 22.8%). CONCLUSIONS: Restricted spirometry is a common finding in population studies. Additional research is needed to better define and describe the mechanisms that lead to restricted spirometry and potential interventions.
Asunto(s)
Enfermedades Pulmonares/diagnóstico , Capacidad Pulmonar Total , Capacidad Vital , Enfermedad Crónica , Estudios de Factibilidad , Femenino , Volumen Espiratorio Forzado , Salud Global , Humanos , Enfermedades Pulmonares/epidemiología , Enfermedades Pulmonares/fisiopatología , Masculino , Prevalencia , Reproducibilidad de los Resultados , Espirometría/métodosAsunto(s)
Coartación Aórtica/genética , Síndrome de Cornelia de Lange/genética , Defectos del Tabique Interventricular/genética , Enfermedades del Prematuro/genética , Coartación Aórtica/diagnóstico , Síndrome de Cornelia de Lange/diagnóstico , Femenino , Estudios de Seguimiento , Defectos del Tabique Interventricular/diagnóstico , Humanos , Recién Nacido , Enfermedades del Prematuro/diagnóstico , FenotipoRESUMEN
For the prevention and control of non-communicable diseases (NCD), an action plan on NCDs is intended to support coordinated, comprehensive and integrated implementation of strategies and evidence-based interventions across individual diseases and risk factors, especially at the national and regional levels by World Health Organization (WHO). The Global Alliance against Chronic Respiratory Diseases (GARD) is making every attempt to align with WHO's non-communicable diseases action plan. GARD activities have been commenced in over 40 countries and in 11 countries an integrated NCD action plan is being prepared or has already been initiated. This integrated approach of GARD has also targeted to GARD Turkey project. The Turkish Ministry of Health has decided to apply this national control program in conformity with other NCD action plans. This article is intended to summarize these integration efforts of GARD Turkey (the National Control Program on Chronic Airway Diseases) with other NCD national programs.
Asunto(s)
Programas Nacionales de Salud , Enfermedades Respiratorias/prevención & control , Organización Mundial de la Salud , Enfermedad Crónica , Política de Salud , Humanos , Enfermedades Respiratorias/patología , TurquíaRESUMEN
BACKGROUND: Vitamin D receptor (VDR) gene is regarded as one of the candidate genes for type 1 diabetes mellitus (T1D) susceptibility and of some genetic factors involved in the development of osteoporosis in this group. STUDY DESIGN: We characterized the VDR gene polymorphism (BsmI, ApaI, TaqI, FokI and Cdx-2 binding site) in a group of Turkish patients with T1D (n=90) and correlated respective VDR genotypes with the bone mass and some parameters of bone turnover. RESULTS: There were no differences in the genotype frequencies of the BsmI, ApaI, TaqI and Cdx-2 polymorphisms in patients and control subjects. We found a significantly higher prevalence of the F allele/the FF genotype in the patients compared to controls (p=0,0031, odds 1.96 (1,27-3,01)). We observed no difference in markers of bone turnover (Serum levels of osteocalcin, PINP and alkaline phosphatase, urinary levels of calcium/ creatinine and N-telopeptid) among different VDR genotypes. No correlation was found between VDR polymorphisms and DEXA measurements of these patients. CONCLUSIONS: Although the FF genotype was found to be a risk factor in a Turkish population, elucidation of this result is necessary in other larger study groups drawn from the same ethnic population.
Asunto(s)
Densidad Ósea , Diabetes Mellitus Tipo 1/genética , Predisposición Genética a la Enfermedad , Polimorfismo Genético , Receptores de Calcitriol/genética , Factor de Transcripción CDX2 , Niño , Preescolar , Femenino , Genotipo , Proteínas de Homeodominio/fisiología , Humanos , Masculino , Transactivadores/fisiología , TurquíaRESUMEN
In order to prevent and control non-communicable diseases (NCDs), the 61st World Health Assembly has endorsed an NCD action plan (WHA resolution 61.14). A package for essential NCDs including chronic respiratory diseases (CRDs) has also been developed. The Global Alliance against Chronic Respiratory Diseases (GARD) is a new but rapidly developing voluntary alliance that is assisting World Health Organization (WHO) in the task of addressing NCDs at country level. The GARD approach was initiated in 2006. GARD Turkey is the first comprehensive programme developed by a government with all stakeholders of the country. This paper provides a summary of indicators of the prevalence and severity of chronic respiratory diseases in Turkey and the formation of GARD Turkey.
Asunto(s)
Política de Salud , Enfermedades Respiratorias/epidemiología , Enfermedades Respiratorias/prevención & control , Organización Mundial de la Salud , Enfermedad Crónica , Humanos , Prevalencia , Enfermedades Respiratorias/patología , Índice de Severidad de la Enfermedad , Turquía/epidemiologíaRESUMEN
The present study was undertaken as part of the first application and evaluation of the BREF (Best Available Techniques; BAT Reference Document) Textile Document within the context of the European Union's Integrated Pollution Prevention and Control (IPPC) Directive to a large scale textile mill in Turkey. The BAT requirements for the denim manufacturing textile mill were selected in cooperation with the factory management. Detailed mass balance calculations were conducted to evaluate the overall effect of the selected BAT options. The initial findings indicated that the adoption of the selected BAT options resulted in considerable savings in water and energy consumption in the mill. Besides the installation of flow meters and use of semi-counter current rinsing in the most water-intensive processes, minimization of wash waters in the water softening plant, reuse of the concentrate stream from the reverse osmosis plant and compressor cooling waters provided a 29.5% reduction in the total specific water consumption of the mill, reaching the lower limits suggested by the BREF Textile Document. In terms of energy consumption, use of waste heat from finishing wastewater streams in heating up the wash waters, heat insulation and maintenance applications in addition to BAT measures taken for water minimization reduced specific energy consumption by 9% achieving the limits set by the BREF Textile Document.
Asunto(s)
Suministros de Energía Eléctrica , Industria Textil , Agua , Difusión de Innovaciones , Unión EuropeaRESUMEN
Published guidelines recommend spirometry to accurately diagnose chronic obstructive pulmonary disease (COPD). However, even spirometry-based COPD prevalence estimates can vary widely. We compared properties of several spirometry-based COPD definitions using data from the international Burden of Obstructive Lung Disease (BOLD)study. 14 sites recruited population-based samples of adults aged > or =40 yrs. Procedures included standardised questionnaires and post-bronchodilator spirometry. 10,001 individuals provided usable data. Use of the lower limit of normal (LLN) forced expiratory volume in 1 s (FEV(1)) to forced vital capacity (FVC) ratio reduced the age-related increases in COPD prevalence that are seen among healthy never-smokers when using the fixed ratio criterion (FEV(1)/FVC <0.7) recommended by the Global Initiative for Chronic Obstructive Lung Disease. The added requirement of an FEV(1) either <80% predicted or below the LLN further reduced age-related increases and also led to the least site-to-site variability in prevalence estimates after adjusting for potential confounders. Use of the FEV(1)/FEV(6) ratio in place of the FEV(1)/FVC yielded similar prevalence estimates. Use of the FEV(1)/FVCAsunto(s)
Volumen Espiratorio Forzado
, Enfermedad Pulmonar Obstructiva Crónica/diagnóstico
, Enfermedad Pulmonar Obstructiva Crónica/fisiopatología
, Espirometría
, Capacidad Vital
, Adulto
, Anciano
, Estudios de Cohortes
, Femenino
, Humanos
, Masculino
, Persona de Mediana Edad
, Guías de Práctica Clínica como Asunto
, Valor Predictivo de las Pruebas
, Prevalencia
, Enfermedad Pulmonar Obstructiva Crónica/epidemiología
, Reproducibilidad de los Resultados
, Estudios Retrospectivos
, Encuestas y Cuestionarios
RESUMEN
Proper cell attachment and distribution, and thus stronger association in vivo between a bone implant and native tissue will improve the success of the implant. In this study, the aim was to achieve promotion of attachment and uniform distribution of rat mesenchymal stem cell-derived osteoblasts by introducing chemical and topographical cues on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) film surfaces. As the chemical cues, either alkaline phosphatase was covalently immobilized on the film surface to induce deposition of calcium phosphate minerals or fibrinogen was adsorbed to improve cell adhesion. Microgrooves and micropits were introduced on the film surface by negative replication of micropatterned Si wafers. Both chemical cues improved cell attachment and even distribution on the PHBV films, but Fb was more effective especially when combined with the micropatterns. Cell alignment (<10 degrees deviation angle) parallel to chemically modified microgrooves (1, 3, or 8 microm groove width) and on 10 microm-thick Fb lines printed on the unpatterned films was achieved. The cells on unpatterned and 5 microm-deep micropitted films were distributed and oriented randomly. Results of this study proved that microtopographies on PHBV can improve osseointegration when combined with chemical cues, and that microgrooves and cell adhesive protein lines on PHBV can guide selective osteoblast adhesion and alignment.
Asunto(s)
Osteoblastos/citología , Poliésteres/metabolismo , Naranja de Acridina , Animales , Adhesión Celular , Recuento de Células , Células Cultivadas , Osteoblastos/ultraestructura , Poliestirenos/metabolismo , Ratas , Ratas Sprague-Dawley , Propiedades de SuperficieRESUMEN
OBJECTIVES: The aim of this study is to evaluate the expired-air carbon monoxide level which relates to the severity of inflammation in patients with chronic obstructive pulmonary disease (COPD). DESIGN: Cross sectional study. SETTING: Cukurova University, Faculty of Medicine, Department of Chest Disease, Out-patient clinic. PATIENTS: The characteristics of patients enrolled in this study were following; 20 ex-smokers with stable COPD (mean age: 68.8 +/- 7.2 years, FEV1: 45.6 +/- 16.6% of predicted), 22 current smokers with stable COPD (mean age: 58.7 +/- 8.2 years, FEVI: 57.5 +/- 20.9% of predicted), 20 healthy smokers (mean age: 55.916.0 years, FEVI: 86.7 +/- 14.2% of predicted), and 20 healthy non-smokers (mean age: 60.8 +/- 9.2 years, FEV1: 95.3 +/- 13.5% of predicted). INTERVENTION: CO level was measured in expired-air. MEAAUREMENT AND RESULTS: The measurement of expired-air CO level was measured by DisCOver, carbon monoxide analyser. It is known that the level of expired-air carbon monoxide in healthy smokers (11.8 +/- 6.4 ppm) and in current smokers with COPD (11.1 +/- 7.4 ppm) is higher than in healthy non-smokers (1.7:0.7 ppm) and in ex-smokers with COPD (2.0 +/- 1.8 ppm) (p = 0.0001). CONCLUSION: We assumed that the level of expired-air carbon monoxide may not useful in assessing the severity of inflammation in COPD (Tab. 1, Fig. 2, Ref. 23).
Asunto(s)
Pruebas Respiratorias , Monóxido de Carbono/análisis , Enfermedad Pulmonar Obstructiva Crónica/patología , Anciano , Femenino , Volumen Espiratorio Forzado , Humanos , Inflamación , Masculino , Persona de Mediana Edad , Enfermedad Pulmonar Obstructiva Crónica/metabolismo , Enfermedad Pulmonar Obstructiva Crónica/fisiopatologíaRESUMEN
Before this study, the human norepinephrine transporter (hNET) was the only member of the biogenic amine neurotransmitter transporter family that had not been demonstrated to be a functional homo-oligomer. Here, using two forms of the transporter, I155C and hNET-myc, with distinct antigenicity and inhibitor sensitivity, we demonstrated that hNET exists as a homo-oligomer. hNET I155C is a functional mutant and is sensitive to inactivation by the sulfhydryl reagent [2-(trimethylammonium)ethyl]methanethiosulfonate, while hNET-myc is resistant to inactivation by this reagent. Coimmunoprecipitation of these two forms demonstrated that a physical interaction exists between norepinephrine transporter monomers. Further characterization of this physical interaction has revealed that the activity of norepinephrine transporters depends on interactions between monomers. Because norepinephrine transporters and serotonin transporters are the only two members of the neurotransmitter transporter family endogenously expressed in the cell membrane of the same cells, placental syncytiotrophoblasts, we tested the ability of norepinephrine transporters and serotonin transporters to associate and function in a hetero-oligomeric form. Similarly, coexpression of hNET-myc with serotonin transporter-FLAG showed a physical interaction in coimmunoprecipitation assays. However, coexpression of serotonin and norepinephrine transporters did not sensitize norepinephrine transporter activity to inhibition by citalopram, a selective serotonin transport inhibitor. Thus, the norepinephrine transporter-serotonin transporter physical association did not produce functional consequences. Based on this, we propose that the transporters for biogenic amine neurotransmitters interact functionally in homo- but not hetero-oligomeric forms.
Asunto(s)
Monoaminas Biogénicas/metabolismo , Proteínas de Transporte de Membrana , Proteínas del Tejido Nervioso , Simportadores/metabolismo , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Secuencia de Bases , Proteínas Portadoras/química , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Membrana Celular/metabolismo , Expresión Génica/fisiología , Células HeLa , Humanos , Glicoproteínas de Membrana/química , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Mesilatos/química , Datos de Secuencia Molecular , Mutación , Proteínas de Transporte de Noradrenalina a través de la Membrana Plasmática , Pruebas de Precipitina , Unión Proteica/fisiología , Proteínas Proto-Oncogénicas c-myc/genética , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Proteínas de Transporte de Serotonina en la Membrana Plasmática , Reactivos de Sulfhidrilo/química , Simportadores/química , Simportadores/genética , TransfecciónRESUMEN
We investigated the oxidant-antioxidant balance and the effect of inhaled corticosteroids on this balance in mild stable asthmatics. Included in the study were 30 mild asthmatic patients (11 male, 19 female, mean age (year) +/- SD: 35.1 +/- 9.7) and 26 healthy adults (7 male, 19 female, mean age (year) +/- SD: 40.8 +/- 13.3). In all study groups, the peripheral venous blood samples were taken for plasma malonyldialdehyde (MDA), a parameter of lipid peroxidation caused by the oxidants, and erythrocyte superoxide dismutase (SOD), an antioxidant enzyme. The mean plasma MDA level was lower in the asthmatic group (5.7 +/- 1.2 nmol/ml) than in the healthy group (6.3 +/- 1.7 nmol/ml); and the mean erythrocyte SOD level was higher in asthmatic group (1086.4 +/- 247.4 U/gHb) than in the healthy group (1028.0 +/- 230.0 U/gHb). However, there were no significant differences in measurements of both plasma MDA levels and erythrocyte SOD enzyme activities between the groups (respectively, p = 0.1 and p = 0.4). When asthmatic patients were divided into subgroups as "inhaled steroid user" and "no inhaled steroid user", no significant differences were observed in the measurements of either plasma MDA level or erythrocyte SOD enzyme activity between the mentioned subgroups. According to the results of our study, we can say that oxidant-antioxidant balance is not significantly affected in mild asthmatics or measurement of plasma level of MDA and erythrocyte SOD enzyme activity is not sensitive to the oxidant-antioxidant balance in mild asthmatics.
Asunto(s)
Antioxidantes/metabolismo , Asma/metabolismo , Oxidantes/metabolismo , Administración por Inhalación , Corticoesteroides/uso terapéutico , Adulto , Asma/tratamiento farmacológico , Asma/fisiopatología , Biomarcadores/sangre , Eritrocitos/efectos de los fármacos , Eritrocitos/enzimología , Femenino , Volumen Espiratorio Forzado/efectos de los fármacos , Volumen Espiratorio Forzado/fisiología , Humanos , Peroxidación de Lípido/efectos de los fármacos , Peroxidación de Lípido/fisiología , Masculino , Malondialdehído/metabolismo , Estrés Oxidativo/efectos de los fármacos , Estrés Oxidativo/fisiología , Valor Predictivo de las Pruebas , Pruebas de Función Respiratoria , Índice de Severidad de la Enfermedad , Pruebas Cutáneas , Superóxido Dismutasa/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Resultado del Tratamiento , TurquíaRESUMEN
The purpose of this study was to verify the kinetic response of the human marrow myeloid progenitor cells to the short term use of GM-CSF and its impact on the therapeutic activity of this three-drug cisplatinum containing regimen in non small cell lung cancer (NSCLC). Sixty patients with stage III-B and IV NSCLC were randomised to receive GM-CSF for 3 days, five days prior to the onset of chemotherapy. The chemotherapy regimen consisted of Mitomycin-C: 6 mg/m2 on day one, Ifosfamide: 2000 mg/m2 days 1 to 3, Mesna: 2000 mg/m2 days 1 to 3, Cisplatinum: 30 mg/m2 days 1 to 3, and was repeated every 4 weeks. All the patients received 30-50 Gy of radiotherapy to the primary and/or metastatic sites. There were positive correlations between stage of the disease, chemosensitivity of the tumor, number of chemotherapy cycles and overall survival (p=0.000). Administration of GM-CSF was an independent prognostic parameter in locally advanced and metastatic disease (p=0.041). In the GM-CSF receiving arm more courses could be given (117 versus 99, p=0.0415), and less courses were postponed (6 versus 22). In this arm, the mean of granulocyte nadir was higher (p=0.033) and mean time to granulocyte recovery became shorter (p=0.001) as the number of chemotherapy cycles increased. It was concluded that, dose intensification with GM-CSF prophylaxis is benefical in increasing the treatment tolerability by decreasing the intensity of granulocytopenia as well as providing rapid recovery.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Carcinoma de Pulmón de Células no Pequeñas/tratamiento farmacológico , Factor Estimulante de Colonias de Granulocitos y Macrófagos/uso terapéutico , Neoplasias Pulmonares/tratamiento farmacológico , Adulto , Anciano , Protocolos de Quimioterapia Combinada Antineoplásica/efectos adversos , Carcinoma de Pulmón de Células no Pequeñas/mortalidad , Carcinoma de Pulmón de Células no Pequeñas/patología , Carcinoma de Pulmón de Células no Pequeñas/radioterapia , Cisplatino/administración & dosificación , Esquema de Medicación , Femenino , Células Madre Hematopoyéticas/patología , Humanos , Ifosfamida/administración & dosificación , Neoplasias Pulmonares/mortalidad , Neoplasias Pulmonares/patología , Neoplasias Pulmonares/radioterapia , Masculino , Mesna/administración & dosificación , Persona de Mediana Edad , Mitomicina/administración & dosificación , Proteínas Recombinantes , Tasa de SupervivenciaRESUMEN
Expansion of trinucleotide repeats within genes is well known to cause pathological conditions in humans. Here we report a large number of genes containing simple sequence repeats (SSR) from the brain of channel catfish, of which a homologue of the RAD23B gene was found to include (CCA) trinucleotide repeats within its coding region. Because of the importance of the RAD23B gene in the nucleotide excision repair (NER) system, the catfish RAD23B locus was further characterized. The (ACC) repeats encode a polythreonine (T) tract within the catfish RAD23B gene that is absent from the previously cloned human and mouse genes. A survey of the allele variation at the locus indicated the existence of variable microsatellite repeats in the NER RAD23B gene, suggesting that the trinucleotide repeats are expanding or shrinking. The majority of individuals harbor 10 (ACC) repeats within the RAD23B gene, but alleles with 8 and 11 repeats were also detected. The (ACC) repeats are limited to only channel catfish and the closely related blue catfish, but are absent from flathead catfish and the cloned human and mouse genes, suggesting that the microsatellite invasion into the RAD23B gene is a recent event in evolution.
Asunto(s)
Encéfalo/metabolismo , Proteínas de Unión al ADN/genética , Repeticiones de Microsatélite , Expansión de Repetición de Trinucleótido , Alelos , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Southern Blotting , Bagres , Reparación del ADN , Enzimas Reparadoras del ADN , ADN Complementario/metabolismo , Evolución Molecular , Etiquetas de Secuencia Expresada , Biblioteca de Genes , Humanos , Ratones , Datos de Secuencia Molecular , Plásmidos/metabolismo , Reacción en Cadena de la Polimerasa , Homología de Secuencia de Aminoácido , Distribución TisularRESUMEN
Analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and development of resources useful for functional genomics studies. As part of the transcriptome analysis in channel catfish (Ictalurus punctatus), we have conducted EST analysis using a cDNA library made from the head kidney. We analysed 2228 EST clones. Orthologues were established for 1495 (67.1%) clones representing 748 genes, of which 545 (36.5%) clones were singletons. The remaining 733 (32.9%) clones represent unknown gene clones, for which the number of genes has not yet been determined.
Asunto(s)
Etiquetas de Secuencia Expresada , Ictaluridae/genética , Riñón/fisiología , Animales , Secuencia de Bases , ADN Complementario/química , Perfilación de la Expresión Génica , Ictaluridae/metabolismo , Riñón/metabolismo , Datos de Secuencia Molecular , ARN/química , ARN/aislamiento & purificación , Análisis de Secuencia de ADN , Homología de Secuencia de Ácido NucleicoRESUMEN
Expressed sequence tag (EST) analysis was conducted using a complementary DNA (cDNA) library made from the brain mRNA of channel catfish (Ictalurus punctatus). As part of our transcriptome analysis in catfish to develop molecular reagents for comparative functional genomics, here we report analysis of 1201 brain cDNA clones. Of the 1201 clones, 595 clones (49.5%) were identified as known genes by BLAST searches and 606 clones (50.5%) as unknown genes. The 595 clones of known gene products represent transcripts of 251 genes. These known genes were categorized into 15 groups according to their biological functions. The largest group of known genes was the genes involved in translational machinery (21.4%) followed by mitochondrial genes (6.2%), structural genes (3.1%), genes homologous to sequences of unknown functions (2.3%), enzymes (2.7%), hormone and regulatory proteins (2.5%), genes involved in immune systems (2.1%), genes involved in sorting, transport, and metal metabolism (1.8%), transcriptional factors and DNA repair proteins (1.6%), proto-oncogenes (1.2%), lipid binding proteins (1.2%), stress-induced genes (0.7%), genes homologous to human genes involved in mental diseases (0.6%), and development or differentiation-related genes (0.3%). The number of genes represented by the 606 clones of unknown genes is not known at present, but the high percentage of clones showing no homology to any known genes in the GenBank databases may indicate that a great number of novel genes exist in teleost brain.
Asunto(s)
Perfilación de la Expresión Génica , Ictaluridae/genética , Animales , Encéfalo/metabolismo , ADN Complementario/química , ADN Complementario/genética , ADN Mitocondrial/genética , Etiquetas de Secuencia Expresada , Regulación de la Expresión Génica , Biblioteca de Genes , Datos de Secuencia Molecular , ARN de Transferencia de Valina/genética , Proteínas Ribosómicas/genética , Análisis de Secuencia de ADN , Transcripción GenéticaRESUMEN
This study was aimed to evaluate the effect of ifosfamide, cisplatin and etoposide (ICE) combined chemotherapy in small cell lung cancer (SCLC), and to test the feasibility of adding recombinant human granulocyte colony-stimulating factor (rhG-CSF) to aggressive chemotherapy. Thirty consecutive, previously untreated, patients with SCLC (17 with limited disease and 13 with extensive disease) entered this study. The ICE regimen consisted of ifosfamide (I) 4 g/m2 i.v. with same dose mesna i.v. on first day, cisplatin (C) 25 mg/m2 i.v. on days 1 to 3 and etoposide (E) 100 mg/m2 i.v. on days 1 to 3. A total of 30 MU rhG-CSF i.v. were given from day 7 to 14 if WBC were lower than 3000 x 10(6)/L, neutrophils were lower than 1000 x 10(6)/L. Overall response (OR) rate was 93% with a complete response (CR) rate of 23%. Median survival was 12 months [95% confidence interval (CI): 11-14] and median response duration was 10 months [95% CI: 8-10]. Thirty-seven percent of patients had grade 3 neutropenia, 40% had grade 3 anemia, and 1% had grade 2 thrombocytopenia. Nonhematologic toxicity was mild with nausea and vomiting being the most common. RhG-CSF, which reduced leukopenic nadirs and shortened the neutropenic period, was also well tolerated. This chemotherapy protocol seems to be active, well tolerated and is currently being compared with various conventional chemotherapies.