Extraction and characterization of exopolysaccharides from Lactiplantibacillus plantarum strain PRK7 and PRK 11, and evaluation of their antioxidant, emulsion, and antibiofilm activities.

Exopolysaccharides (EPS) are produced by probiotic bacteria Lactiplantibacillus plantarum PRK7 and L. plantarum PRK11. The structure of EPS-7 and EPS-11 was characterized by Fourier-transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), gas chromatography-mass spectroscopy (GCMS), and thermogravimetric analysis (TGA). Further, in in vitro studies antioxidant, emulsion, and antibiofilm activity were investigated. The FTIR spectrum confirmed the presence of polysaccharides in EPS-7 and EPS-11, with absorbance at 1654.93 and 1655.33 cm-1, respectively. H1 NMR further confirmed the presence of glucose, galactose, xylose, and mannose. Sugar derivatives in EPS-7 and EPS-11 were further confirmed with GCMS. The SEM analysis revealed that EPS-7 had a weblike structure and EPS-11 had a smooth porous layer. The result of the TGA revealed that EPS-7 and EPS-11 had greater thermal stability at 319.1 and 300.1 °C, respectively. Furthermore, EPS-7 and EPS-11 showed a good percentage of free radical scavenging in DPPH (89.77 % and 93.1 %), ABTS (57.65 % and 58.63 %), hydroxyl radical scavenging (44.46 % and 40.308 %), and reducing power assay. The emulsion activity was confirmed with edible oils such as coconut oil, sesame oil, almond oil, castor oil, and neem oil. The highest emulsion activity for EPS-7 and EPS-11 was found with coconut and castor oil. In addition, the antibiofilm activity against pathogens revealed that EPS can prevent biofilm formation. Thus, it was found that EPS-7 and EPS-11 possess good structural characteristics and their biological activity makes them ideal for applications in the food and pharmaceutical industry.

Green synthesis of stable antioxidant, anticancer and photocatalytic activity of zinc oxide nanorods from Leea asiatica leaf.

The present work reveals zinc oxide nanorods (ZnO NRs) obtained via green synthesis approach utilizing phytochemicals present in the Leea asiatica plant. The synthesized ZnO NRs was confirmed by various characterization methods like X-ray diffraction, FTIR, SEM-EDX, UV-vis, TEM and Raman spectra analysis. The obtained nanorods show less particle size with a distinctive high surface area. The photodegradation activity of obtained ZnO NRs shows 81.3 % of dye degradation at high concentration whereas 42.9 % in low concentration and also antioxidant test shows a better result for free radical scavenging. To understand the safety of the ZnO NRs zebrafish embryos was utilized as a model and found to be less toxic after assessment. Furthermore, to understand the anticancer activity of ZnO NRs it was assessed against breast cancer cell line through MTT, XTT, NRU, and LDH assay. The ZnO NRs exposed against breast cancer cell line (MCF-7) had resulted in necrosis and apoptosis which was further confirmed by genotoxicity assay. Also, the molecular mechanisms behind the necrosis and apoptosis are also discussed to show the involvement of ZnO NRs towards breast cancer cells (MCF-7). The present study on ZnO NRs gives a wide spectrum understanding of using ZnO NRs as a potential drug for human breast cancer by showing its physiological pathways as well as a novel way to synthesize ZnO NRs using Leea asiatica plant extract.