RESUMEN
OBJECTIVE: To isolate the wild-type virulent phage of Helicobacter pylori (Hp) and simulate the treatments in vitro to investigate the methods for oral Hp-assisted penetration of the phage through the gastric barrier and offspring phage release for infection and treatment of gastrointestinal Hp. METHODS: The Hp strain was cultured with the candle cylinder method and the virulent phage was isolated by single plate or double plate experiment. A simulated gastric juice was applied and the bactericidal effect of the phage was tested with double flats experiment. RESULTS: After a 1.5-h treatment in simulated gastric juice, the orally derived Hp-borne phage was still capable of forming plaques while the control phage was not. CONCLUSION: The oral Hp can help the phage resist the gastric juice and then infect the gastrointestinal Hp.
Asunto(s)
Bacteriófagos/aislamiento & purificación , Bacteriófagos/fisiología , Tracto Gastrointestinal/microbiología , Infecciones por Helicobacter/terapia , Helicobacter pylori/virología , Humanos , VirulenciaRESUMEN
OBJECTIVE: To discuss the possible mechanism of drug resistance transmission between Staphylococcus and Escherichia coli. METHODS: The chloramphenicol resistance plasmid of Staphylococcus aureus was extracted to transform the sensitive Escherichia coli, and the drug-resistant Escherichia coli were screened by drug sensitivity test. RESULTS: The drug-resistant Escherichia coli were successfully obtained. CONCLUSION: Staphylococcus may have a natural shuttle plasmid of drug resistance, which can transform Escherichia coli under specific conditions.