A biphasic drug-releasing microneedle with ROS scavenging and angiogenesis for the treatment of diabetic ulcers.

Diabetic ulcers are one of the common complications in diabetic patients. Delayed wound healing is associated with persistent pro-inflammatory M1 polarization, reduced angiogenesis and increased reactive oxygen species (ROS) in the microenvironment. Wound healing consists of multiple phases and therefore requires treatment tailored to each phase. In this study, a biphasic drug-releasing microneedle (MN) was fabricated to achieve early ROS scavenging and late accelerated angiogenesis to promote wound healing. Vascular endothelial growth factor (VEGF) was first encapsulated in methacryloylated sulfonated chitosan (SCSMA) microspheres (V@MP), and then V@MP was loaded into hyaluronic acid (HA) microneedles along with cerium dioxide nanoparticles (CONPs). Rapid dissolution of HA rapidly releases the CONPs to clear ROS, whereas the V@MP stays in the wound. SCSMA slow degradation prolongs the release of VEGF, thereby promoting angiogenesis. In vitro and in vivo studies have shown that this biphasic drug-releasing smart microneedle improves cell proliferation and migration, effectively scavenges ROS, promotes angiogenesis and tissue regeneration, and synergistically promotes M2 macrophage polarization. It provides a new delivery mode for nano-enzymes and growth factors that could be multifunctional and synergistic in the treatment of diabetic ulcers. STATEMENT OF SIGNIFICANCE: In our study, we present a microneedle (V@MP/C@MN) that can release drugs biphasically, which showed good repair ability in diabetic ulcer model. Large amounts of CONPs were rapidly released to alleviate oxidative stress during the inflammation of the wound, and V@MP stayed in the wound for a long period of time to release VEGF and promote angiogenesis in the late stage of wound healing. The results indicated that V@MP/C@MN could promote cell proliferation and migration, effectively scavenge ROS, promote angiogenesis and tissue regeneration, and synergistically promote M2 macrophage polarization, which could play a multifunctional and synergistic role in the treatment of diabetic ulcers.

Active balancing strategy for AUV power battery pack based on PSO-PID algorithm.

In this paper, the battery inconsistency equalisation strategy is investigated and a novel fusion model based on equivalent circuit models is proposed. The three equivalent circuit models, 1RC, 2RC and PNGV, are weighted and fused by BP neuron network, which realizes the complementary advantages of the three equivalent circuit models. Even though the estimated values of all three models are lower than the true value, they can still be close to the true value after reasonable weight allocation. With reference to the open-source DST dynamic operating test data from the Advanced Battery Association of America, a comparison is made with the three common equivalent circuit models of 1RC, 2RC and PNGV. It is found that the proposed novel fusion model has the highest estimation accuracy with a maximum error of only 0.00947; the RMSE is 0.00217. The 1RC equivalent circuit model has the worst accuracy with a maximum error of 0.10145 and an RMSE of 0.02153. The 2RC and PNGV models have accuracies between the two. Then an active equalisation system is constructed to realise the charge equalisation of multiple unbalanced single batteries by distributed power supply. The PSO-PID strategy is used to control the topology circuit for adaptive equalisation. The equalisation effect of different sized battery packs is verified by simulation. Compared with the traditional logic control strategy, the method is simple and effective, and the equalisation effect is better as the number of inconsistent battery cells increases. In a battery pack with five initial SOC inconsistencies, the inter-cell variability is quickly equalised. When dynamic disturbances are introduced into the system, the algorithm also keeps the battery packs within the equalisation range with an average variance of 0.0016.

Preparation and identification of novel antioxidant peptides from collagen hydrolysate of sheep hoof assisted by ultrasound.

In this study, different ultrasound-assisted modes [ultrasonic simultaneous (US) and ultrasonic preconditioning (UP)] of synergistic enzymatic hydrolysis were used to prepare bioactive peptides of sheep hoof collagen. The 2, 2-diphenyl - 1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2 '-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity and metal chelating capacity of sheep's hoof collagen antioxidant peptides (SCPs) (at 1 mg/mL) prepared at 20 min of treatment in US treatment mode (US-20) were 48.56 ±â€¯0.68 %, 51.97 ±â€¯1.15 % and 65.58 ±â€¯1.36 %, respectively, which were higher compared with the control and UP groups. Using LC-MS/MS analysis, 9336, 11,527, and 11,909 peptide sequences were identified from collagen hydrolysate by C, UP-20, and US-20, respectively. The peptides ACEDAPPSAAHFR and FGFEVGPACFLG with high bioactivity were screened using computer analysis. Molecular docking results revealed that hydrogen bonding and hydrophobic interactions between the two peptide sequences with DPPH and ABTS radicals may be responsible for their antioxidant properties. Therefore, we have optimized the extraction of bioactive peptides from sheep hoof collagen using ultrasound-assisted enzymatic hydrolysis, which is helpful for the high-value utilisation of sheep hoof by-products and the extraction of foodborne antioxidant peptides.

Extending Dynamic Blind Interaction: Microgap Discharge-Induced Flexible Virtual Electrotactile Braille.

Braille is an essential implement for the blind to communicate with outside, but traditional Braille is limited to a paper-based format that cannot directly provide real-time word information. In this work, a flexible virtual electrotactile Braille is proposed that can benefit the blind from blocked interaction. The Braille interface, S-shaped wires and a sphere electrode with a textile fingerstall integrated by silicone, offers flexibility and simultaneously generates the microgap through textile cracks, which achieves virtual electrotactile sensation by electrostatic discharge. Powered by a high-voltage triboelectric generator of 10.2 kV designed through the charge accumulation and induction strategy, the electrotactile stimulation is realized with a microgap discharge of only 40 µA current induced on the finger. A dynamic electrotactile Braille is finally assembled, controlled by a programmable relay array. The strategies of short circuit and voice reminder are employed, so that the recognition of dynamic Braille letters is realized with spatiotemporal electrotactile stimulation and high recognition accuracy. This virtual electrotactile Braille brings convenience for the blind to access the information world and illustrates its applications to promote virtual electrotactility in this special community.

Microbial diversity across tea varieties and ecological niches: correlating tea polyphenol contents with stress resistance.

Introduction: Microorganisms exhibit intricate interconnections with tea plants; however, despite the well-established role of microorganisms in crop growth and development, research on microbes within the tea plant remains insufficient, particularly regarding endophytic microorganisms. Methods: In this study, we collected samples of leaves and rhizosphere soils from 'Zhuyeqi', 'Baojing Huangjincha#1', 'Baiye#1', and 'Jinxuan' varieties planted. Results: Our analyses revealed significant variations in tea polyphenol contents among tea varieties, particularly with the 'Zhuyeqi' variety exhibiting higher levels of tea polyphenols (>20% contents). Microbiome studies have revealed that endophytic microbial community in tea plants exhibited higher host specificity compared to rhizospheric microbial community. Analyses of across-ecological niches of the microbial community associated with tea plants revealed that soil bacteria serve as a significant reservoir for endophytic bacteria in tea plants, Bacillus may play a crucial role in shaping the bacterial community across-ecological niche within the tea plants with higher tea polyphenol levels. In the aforementioned analyses, the microbial community of 'Zhuyeqi' exhibited a higher degree of host specificity for leaf endophytic microorganisms, the topological structure of the co-occurrence network is also more intricate, harboring a greater number of potential core microorganisms within its nodes. A closer examination was conducted on the microbial community of 'Zhuyeqi', further analyses of its endophytic bacteria indicated that its endophytic microbial community harbored a greater abundance of biomarkers, particularly among bacteria, and the enriched Methylobacterium and Sphingomonas in 'Zhuyeqi' may play distinct roles in disease resistance and drought resilience in tea plants. Conclusion: In summary, this study has shed light on the intricate relationships of tea plant varieties with their associated microbial communities, unveiling the importance of microorganisms and tea varieties with higher tea polyphenols, and offering valuable insights to the study of microorganisms and tea plants.

Epoxy-based multifunctional re-bondable polymer with self-healing, shape memory and superb bonding properties.

Thermoset epoxy resin-based materials are widely used, but their permanent cross-linked network limits their processability and reusability, which can lead to environmental burdens. In this work, by exploiting the weak reactivity of aniline to design appropriate reaction ratios, we achieved a linear link between the epoxy resin and the curing agent. This linear link, along with the crosslinking points provided by the flexibly branched polyurethanes, avoids the inherent brittleness associated with the highly crosslinked network of conventional epoxy resins. As a result, the adhesive exhibits extraordinary improvements in extensibility and toughness. The lap shear strength, tensile strength and elongation at break reach 11.9 MPa, 14.4 MPa and 607 %, respectively. The fracture toughness is as high as 109.6 kJ/m2, far beyond the existing epoxy adhesives. The synergistic effect of disulfide bonds and hydrogen bonds confers the adhesive with self-healing and repeatable bonding characteristics. The multi-level hydrogen bonding and appropriate phase separation structure are key to optimizing toughness, resulting in excellent comprehensive performance. The introduction of polyurethane not only improves toughness but also enhances the interfacial bonding force between the adhesive and the substrate, broadening the scope of applications. The prepared high-performance polymers provide new insights into reusable epoxy adhesives.

Recovering skin-nerve interaction by nanoscale metal-organic framework for diabetic ulcers healing.

Skin-nerve interaction plays an important role in promoting wound healing. However, in diabetic ulcers (DUs), the diabetic periphery neuropathy and excessive levels of reactive oxygen species (ROS) block skin-nerve interaction and further impede the DUs healing. Herein, we developed a nanoscale metal-organic framework loaded with nerve growth factor (NGF/Ce-UiO-66, denoted NGF/CU) for the treatment of DUs. The Ce-UiO-66 (CU) was applied as an antioxidant to scavenge ROS and reduce the inflammatory response while the NGF aided in the recovery of cutaneous nerves to further promote DUs healing. Both in vitro and in vivo experiments revealed the effective ability of NGF/CU for DUs healing. Subsequent RNA sequencing analysis revealed the mechanism that NGF/CU can improve wound healing by inhibiting the NF-κB signaling pathway and recovering the neuroendocrine system of the skin. This strategy of nerve regulation will provide more ideas for the treatment of DUs and other organ injuries.

Inhalable nanoparticles with enhanced cuproptosis and cGAS-STING activation for synergistic lung metastasis immunotherapy.

Due to the insufficient Cu+ accumulation, Cu+ efflux mechanism, and highly immunosuppressive tumor microenvironment (TME) in lung metastasis, the cuproptosis efficacy is limited. Herein, an inhalable nanodevice (CLDCu) is constructed to successfully overcome the drawbacks of cuproptosis. CLDCu consists of a Cu2+-chitosan shell and low molecular weight heparin-tocopherol succinate (LMWH-TOS, LT) core with disulfiram (DSF) loading. The prepared CLDCu can be inhaled and accumulate in large amounts in lung lesions (63.6%) with 56.5 times higher than intravenous injection. Within tumor cells, the mild acidity triggers the co-release of DSF and Cu2+, thus generating bis(diethyldithiocarbamate)-copper (CuET) to block Cu+ efflux protein ATP7B and forming toxic Cu+, leading to enhanced cuproptosis. Meanwhile, the released chitosan cooperates with CLDCu-induced cuproptosis to activate stimulator of interferon genes (STING) pathway, which significantly potentiates dendritic cells (DCs) maturation, as wells as evokes innate and adaptive immunity. In lung metastatic mice model, CLDCu is found to induce cuproptosis and reverse the immunosuppressive TME by inhalation administration. Moreover, CLDCu combined with anti-programmed cell death protein ligand-1 antibody (aPD-L1) provokes stronger antitumor immunity. Therefore, nanomedicine that combines cuproptosis with STING activation is a novel strategy for tumor immunotherapy.

[A review of functional electrical stimulation based on brain-computer interface].

Individuals with motor dysfunction caused by damage to the central nervous system are unable to transmit voluntary movement commands to their muscles, resulting in a reduced ability to control their limbs. However, traditional rehabilitation methods have problems such as long treatment cycles and high labor costs. Functional electrical stimulation (FES) based on brain-computer interface (BCI) connects the patient's intentions with muscle contraction, and helps to promote the reconstruction of nerve function by recognizing nerve signals and stimulating the moving muscle group with electrical impulses to produce muscle convulsions or limb movements. It is an effective treatment for sequelae of neurological diseases such as stroke and spinal cord injury. This article reviewed the current research status of BCI-based FES from three aspects: BCI paradigms, FES parameters and rehabilitation efficacy, and looked forward to the future development trend of this technology, in order to improve the understanding of BCI-based FES.

An atomically controlled insulator-to-metal transition in iridate/manganite heterostructures.

All-insulator heterostructures with an emerging metallicity are at the forefront of material science, which typically contain at least one band insulator while it is not necessary to be. Here we show emergent phenomena in a series of all-correlated-insulator heterostructures that composed of insulating CaIrO3 and insulating La0.67Sr0.33MnO3. We observed an intriguing insulator-to-metal transition, that depends delicately on the thickness of the iridate component. The simultaneous enhancements of magnetization, electric conductivity, and magnetoresistance effect indicate a percolation-type nature of the insulator-to-metal transition, with the percolation threshold can be reached at an exceptionally low volume fraction of the iridate. Such a drastic transition is induced by an interfacial charge transfer, which interestingly alters the electronic and crystalline structures of the bulk region rather than the limited ultrathin interface. We further showcased the central role of effective correlation in modulating the insulator-to-metal transition, by demonstrating that the critical thickness of iridate for triggering the metallic state can be systematically reduced down to a single unit-cell layer.

The causal relationship between 91 inflammatory cytokines and Gestational Diabetes Mmellitus: A bidirectional two-sample Mendelian randomization study.

BACKGROUND: Gestational Diabetes Mellitus (GDM) poses significant risks to maternal and fetal health, yet its precise etiology remains unclear. Observational studies have demonstrated a link between specific inflammatory cytokines and the occurrence of GDM, but the causal relationships remain uncertain. METHODS: Utilizing publicly accessible genetic data, we performed a bidirectional two-sample mendelian randomization (MR) analysis to elucidate the causal association between 91 inflammatory cytokines and GDM. Sensitivity analysis was carried out to evaluate the robustness, heterogeneity, and potential presence of horizontal pleiotropy within the results. RESULTS: Elevated levels of Interleukin-7 (IL7) and Neurturin (NRTN) (OR=1.104, 95 % CI=1.003-1.216, p = 0.042; OR=1.102, 95 % CI=1.023-1.187, p = 0.010), along with decreased levels of Glial cell line-derived neurotrophic factor (GDNF), Interleukin-12 subunit beta (IL12ß), and Interleukin-20 (IL20) (OR=0.911, 95 % CI=0.849-0.979, p = 0.010;OR=0.955, 95 % CI=0.916-0.996, p = 0.033; OR=0.892, 95 % CI=0.819-0.971, p = 0.008), are associated with increased GDM risk. Additionally, GDM occurrence correlates with increased Matrix metalloproteinase-10 (MMP-10) and decreased Interleukin-20 receptor subunit alpha (IL-20Rα) levels (OR=1.042, 95 % CI=1.002-1.084, p = 0.038; OR=0.949, 95 % CI=0.909-0.992, p = 0.021). Sensitivity analyses detected no significant heterogeneity or pleiotropy. CONCLUSION: This study has clarified the causal link between inflammatory cytokines and GDM, thereby enhancing our comprehension of the potential mechanisms involved in GDM pathogenesis. These findings offer new insights into the etiology, diagnosis, and therapeutic strategies for GDM.

A comprehensive strategy of lipidomics and pharmacokinetics based on ultra-high-performance liquid chromatography-mass spectrometry of Shaoyao Gancao Decoction.

Shaoyao Gancao Decoction (SGD), a traditional Chinese medicine, has been proven to have a good liver protection effect, but the mechanism and pharmacodynamic substances of SGD in the treatment of acute liver injury are still unclear. In this study, an ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was established to characterize 107 chemical components of SGD and 12 compounds absorbed in rat plasma samples after oral administration of SGD. Network pharmacology was applied to construct a component-target-pathway network to screen the possible effective components of SGD in acute liver injury. Using lipidomics based on UHPLC-Q-TOF-MS coupled with a variety of statistical analyses, 20 lipid biomarkers were screened and identified, suggesting that the improvement of acute liver injury by SGD was involved in cholesterol metabolism, glycerol-phospholipid metabolism, sphingolipid signaling pathways and fatty acid biosynthesis. In addition, the UHPLC-tandem MS method was established for pharmacokinetics analysis, and 10 potential active components were determined simultaneously within 12 min through the optimization of 0.1% formic acid water and acetonitrile as a mobile phase system. A Pharmacokinetics study showed that paeoniflorin, albiflorin, oxypaeoniflorin, liquiritigenin, isoliquiritigenin, liquiritin, ononin, formononetin, glycyrrhizic acid, and glycyrrhetinic acid as the potential active compounds of SGD curing acute liver injury.

Protocol to establish a demyelinated animal model to study hippocampal neurogenesis and cognitive function in adult rodents.

Cognitive dysfunction is a prevalent feature in multiple sclerosis, a chronic inflammatory demyelinating disease, which may be correlated with the impairment of adult hippocampal neurogenesis. Here, we present a detailed protocol for the induction of cuprizone demyelinated mice to assess the cognitive function and explore the precise mechanisms underlying cognitive deficits in demyelinated hippocampus. We describe steps for behavioral tests, 5-Ethynyl-2'-deoxyuridine (EdU) and bromodeoxyuridine (BrdU) administration, retrovirus packaging and stereotactic injection, hippocampal tissue preparation, and immunofluorescence staining. For complete details on the use and execution of this protocol, please refer to Song et al.1.

Dual-Alpha: a large EEG study for dual-frequency SSVEP brain-computer interface.

BACKGROUND: The domain of brain-computer interface (BCI) technology has experienced significant expansion in recent years. However, the field continues to face a pivotal challenge due to the dearth of high-quality datasets. This lack of robust datasets serves as a bottleneck, constraining the progression of algorithmic innovations and, by extension, the maturation of the BCI field. FINDINGS: This study details the acquisition and compilation of electroencephalogram data across 3 distinct dual-frequency steady-state visual evoked potential (SSVEP) paradigms, encompassing over 100 participants. Each experimental condition featured 40 individual targets with 5 repetitions per target, culminating in a comprehensive dataset consisting of 21,000 trials of dual-frequency SSVEP recordings. We performed an exhaustive validation of the dataset through signal-to-noise ratio analyses and task-related component analysis, thereby substantiating its reliability and effectiveness for classification tasks. CONCLUSIONS: The extensive dataset presented is set to be a catalyst for the accelerated development of BCI technologies. Its significance extends beyond the BCI sphere and holds considerable promise for propelling research in psychology and neuroscience. The dataset is particularly invaluable for discerning the complex dynamics of binocular visual resource distribution.

Chemical Reaction Modulated Low-Dimensional Phase Toward Highly Efficient Sky-Blue Perovskite Light-Emitting Diodes.

Blue perovskite light-emitting diodes (PeLEDs) are crucial avenues for achieving full-color displays and lighting based on perovskite materials. However, the relatively low external quantum efficiency (EQE) has hindered their progression towards commercial applications. Quasi-two-dimensional (quasi-2D) perovskites stand out as promising candidates for blue PeLEDs, with optimized control over low-dimensional phases contributing to enhanced radiative properties of excitons. Herein, the impact of organic molecular dopants on the crystallization of various n-phase structures in quasi-2D perovskite films. The results reveal that the highly reactive bis(4-(trifluoromethyl)phenyl)phosphine oxide (BTF-PPO) molecule could effectively restrain the formation of organic spacer cation-ordered layered perovskite phases through chemical reactions, simultaneously passivate those uncoordinated Pb2+ defects. Consequently, the prepared PeLEDs exhibited a maximum EQE of 16.6 % (@ 490 nm). The finding provides a new route to design dopant molecules for phase modulation in quasi-2D PeLEDs.

S-(N,N-diethyldithiocarbamoyl)-N-acetyl-l-cysteine for the treatment of non-small cell lung cancer through regulating NF-κB signalling pathway without neurotoxicity.

The discovery of novel targeted agents for non-small cell lung cancer (NSCLC) remains an important research landscape due to the limited efficacy, side effects and drug resistance of current treatment options. Among many repurposed drugs, disulphiram (DSF) has shown the potential to target tumours. However, its unpleasant neurotoxicity greatly limits its use. A DSF derivative, S-(N,N-diethyldithiocarbamoyl)-N-acetyl-l-cysteine (DS-NAC), was synthesised against NSCLC. The therapeutic effects, mechanism and toxicities of DS-NAC were evaluated in A549 and H460 cells and the mouse model of in situ lung cancer. The in vitro results exhibited that DS-NAC had potent anti-proliferation, apoptotic, anti-metastasis and epithelial-mesenchymal transition (EMT) inhibition effects. In the orthotopic lung cancer mouse model, therapeutic effects of DS-NAC were better than those of DSF and were similar to docetaxel (DTX). Also, results from western blot and immunohistochemistry showed that DS-NAC in combination with copper exerted therapeutic effects via regulating NF-κB signalling pathway and ROS-related proteins such as HIF-1α, Nrf2 and PKC-δ rather than regulating ROS level directly. Moreover, the safety evaluation study showed that DS-NAC had low haematologic and hepatic toxicities in comparison with DTX as well as low neurological toxicity compared with DSF. DS-NAC could be a promising anti-lung cancer agent with a favourable safety profile.

[Latest Findings on the Role of α-Ketoglutarate in Metabolic Syndrome]. / α-é ®æˆŠäºŒé ¸åœ¨ä»£è°¢ç»¼åˆå¾ä¸­çš„ç ”ç©¶è¿›å±•.

Alpha-ketoglutarate (α-KG), an endogenous intermediate of the tricarboxylic acid cycle, is involved in a variety of cellular metabolic pathways. It serves as an energy donor, a precursor of amino acid biosynthesis, and an epigenetic regulator. α-KG plays physiological functions in immune regulation, oxidative stress, and anti-aging as well. In recent years, it has been reported that the level of α-KG in the body is closely associated with metabolic syndrome, including obesity, hyperglycemia, and other pathological factors. Exogenous supplementation of α-KG improves obesity, blood glucose levels, and cardiovascular disease risks associated with metabolic syndrome. Furthermore, α-KG regulates the common pathological mechanisms of metabolic syndrome, suggesting the potential application prospect of α-KG in metabolic syndrome. In order to provide a theoretical basis for further exploration of the application of α-KG in metabolic syndrome, we focused on α-KG and metabolic syndrome in this article and summarized the latest research progress in the role of α-KG in improving the pathological condition and disease progression of metabolic syndrome. For the next step, researchers may focus on the co-pathogenesis of metabolic syndrome and investigate whether α-KG can be used to achieve the therapeutic goal of "homotherapy for heteropathy" in the treatment of metabolic syndrome.

Surficial Homogenic Effect Enables Highly Stable Deep-Blue Perovskite Light-Emitting Diodes.

The device performance of deep-blue perovskite light-emitting diodes (PeLEDs) is primarily constrained by low external quantum efficiency (EQE) especially poor operational stability. Herein, we develop a facile strategy to improve deep-blue emission through rational interface engineering. We innovatively reported the novel electron transport material, 4,6-Tris(4-(diphenylphosphoryl)phenyl)-1,3,5-triazine (P-POT2T), and utilized a sequential wet-dry deposition method to form homogenic gradient interface between electron transport layer (ETL) and perovskite surface. Unlike previous reports that achieved carrier injection balance by inserting new interlayers, our strategy not only passivated uncoordinated Pb in the perovskite via P=O functional groups but also reduced interfacial carrier recombination without introducing new interfaces. Additionally, this strategy enhanced the interface contact between the perovskite and ETL, significantly boosting device stability. Consequently, the fabricated deep-blue PeLEDs delivered an external quantum efficiency (EQE) exceeding 5% (@ 460 nm) with an exceptional halftime extended to 31.3 minutes. This straightforward approach offers a new strategy to realize highly efficient especially stable PeLEDs.

In Situ Formation of Compound Eye-like SAN-OSB Composite Microspheres by Melt-Blending Method: Enhancing Multiple-Scattering Effect.

The preparation of novel structures of light-diffusing particles is currently a research focus in the field of light-diffusing materials. This study, conducted by the common melt-blending process, controlled thermodynamic and kinetic factors to distribute smaller-sized organic silica bead (OSB) particles at the interface between a polycarbonate (PC) matrix and spherical island-phase styrene-acrylonitrile copolymer (SAN) for the in situ formation of compound eye-like microspheres with SAN as "large eyes" and OSBs as "small eyes". Through the multiple-scattering effects of these compound eye-like microspheres, these light-diffusing materials significantly improved the haze, scattering range, and light-shielding capabilities while maintaining high transmittance. Specifically, the PC/SAN-OSB light-scattering materials achieved a haze of 100% with an OSB content of only 0.17%, maintaining a transmittance of 88%. Compared with the PC/OSB system with the same level of haze, the addition of OSB was reduced by 88%. Therefore, this study achieved exceptionally effective light-diffusing materials through a simple, environmentally friendly, and low-cost preparation method, suitable for the scalable production of light-diffusing materials in new display and lighting fields.

Design, Synthesis and Preliminary Bioactivity Evaluation of N-Acetylcysteine Derivatives as Antioxidative and Anti-Inflammatory Agents.

N-acetylcysteine (NAC) is a commonly used mucolytic agent and antidote for acetaminophen overdose. For pulmonary diseases, NAC exhibits antioxidative properties, regulates cytokine production, reduces apoptosis of lung epithelial cells, and facilitates the resolution of inflammation. However, the efficacy of NAC in clinical trials targeting different pathological conditions is constrained by its short half-life and low bioavailability. In the present study, a series of NAC derivatives were designed and synthesized to further enhance its pharmacological activity. Structure-activity relationship (SAR) studies were conducted to optimize the activating groups. In vitro evaluations revealed that compounds 4 r, 4 t, 4 w, and 4 x exhibited superior antioxidative and anti-inflammatory activities compared to the positive controls of NAC and fudosteine. The ADME prediction analysis indicated that these compounds exhibited a favorable pharmacological profile. In-vivo experiments with compound 4 r demonstrated that the high-dose group (80 mg/kg) exhibited improved therapeutic effects in reversing the HPY level in mice with pulmonary fibrosis compared to the NAC group (500 mg/kg), further proving its superior oral bioavailability and therapeutic effect compared to NAC.