RESUMEN
Organisms adjust their physiology to cope with environmental fluctuations and maintain fitness. These adaptations occur via genetic changes over multiple generations or through acclimation, a set of reversible phenotypic changes that confer resilience to the individual. Aquatic organisms are subject to dramatic seasonal fluctuations in water salinity, which can affect the function of lateral line mechanosensory hair cells. To maintain hair cell function when salinity decreases, ion-regulating cells, Neuromast-associated ionocytes (Nm ionocytes), increase in number and invade lateral line neuromasts. How environmental changes trigger this adaptive differentiation of Nm ionocytes and how these cells are specified is still unknown. Here, we identify Nm ionocyte progenitors as foxi3a/foxi3b-expressing skin cells and show that their differentiation is associated with sequential activation of different Notch pathway components, which control ionocyte survival. We demonstrate that new Nm ionocytes are rapidly specified by absolute salinity levels, independently of stress response pathways. We further show that Nm ionocyte differentiation is selectively triggered by depletion of specific ions, such as Ca2+ and Na+/Cl-, but not by low K+ levels, and is independent of media osmolarity. Finally, we demonstrate that hair cell activity plays a role in Nm ionocyte recruitment and that systemic factors are not necessary for Nm ionocyte induction. In summary, we have identified how environmental changes activate a signaling cascade that triggers basal skin cell progenitors to differentiate into Nm ionocytes and invade lateral line organs. This adaptive behavior is an example of physiological plasticity that may prove essential for survival in changing climates.
RESUMEN
Loss of sensory hair cells (HCs) in the mammalian inner ear leads to permanent hearing and vestibular defects, whereas loss of HCs in zebrafish results in their regeneration. We used single-cell RNA sequencing (scRNA-seq) to characterize the transcriptional dynamics of HC regeneration in zebrafish at unprecedented spatiotemporal resolution. We uncovered three sequentially activated modules: first, an injury/inflammatory response and downregulation of progenitor cell maintenance genes within minutes after HC loss; second, the transient activation of regeneration-specific genes; and third, a robust re-activation of developmental gene programs, including HC specification, cell-cycle activation, ribosome biogenesis, and a metabolic switch to oxidative phosphorylation. The results are relevant not only for our understanding of HC regeneration and how we might be able to trigger it in mammals but also for regenerative processes in general. The data are searchable and publicly accessible via a web-based interface.
Asunto(s)
Análisis de la Célula Individual , Pez Cebra , Animales , Expresión Génica , Perfilación de la Expresión Génica , Cabello , Mamíferos/genética , Pez Cebra/genéticaRESUMEN
Mammalian inner ear and fish lateral line sensory hair cells (HCs) detect fluid motion to transduce environmental signals. Actively maintained ionic homeostasis of the mammalian inner ear endolymph is essential for HC function. In contrast, fish lateral line HCs are exposed to the fluctuating ionic composition of the aqueous environment. Using lineage labeling, in vivo time-lapse imaging and scRNA-seq, we discovered highly motile skin-derived cells that invade mature mechanosensory organs of the zebrafish lateral line and differentiate into Neuromast-associated (Nm) ionocytes. This invasion is adaptive as it is triggered by environmental fluctuations. Our discovery of Nm ionocytes challenges the notion of an entirely placodally derived lateral line and identifies Nm ionocytes as likely regulators of HC function possibly by modulating the ionic microenvironment. Nm ionocytes provide an experimentally accessible in vivo system to study cell invasion and migration, as well as the physiological adaptation of vertebrate organs to changing environmental conditions.
Asunto(s)
Adaptación Fisiológica , Movimiento Celular , Ambiente , Homeostasis , Sistema de la Línea Lateral/citología , Pez Cebra/fisiología , Animales , Biomarcadores/metabolismo , Recuento de Células , Factores de Transcripción Forkhead/metabolismo , Branquias/citología , Células Ciliadas Auditivas/citología , Concentración de Iones de Hidrógeno , Imagenología Tridimensional , Receptores Notch/metabolismo , Salinidad , Transducción de Señal , Piel/citología , Proteínas de Pez Cebra/metabolismoRESUMEN
Loss of sensory hair cells leads to deafness and balance deficiencies. In contrast to mammalian hair cells, zebrafish ear and lateral line hair cells regenerate from poorly characterized support cells. Equally ill-defined is the gene regulatory network underlying the progression of support cells to differentiated hair cells. scRNA-Seq of lateral line organs uncovered five different support cell types, including quiescent and activated stem cells. Ordering of support cells along a developmental trajectory identified self-renewing cells and genes required for hair cell differentiation. scRNA-Seq analyses of fgf3 mutants, in which hair cell regeneration is increased, demonstrates that Fgf and Notch signaling inhibit proliferation of support cells in parallel by inhibiting Wnt signaling. Our scRNA-Seq analyses set the foundation for mechanistic studies of sensory organ regeneration and is crucial for identifying factors to trigger hair cell production in mammals. The data is searchable and publicly accessible via a web-based interface.
Asunto(s)
Proliferación Celular , Factores de Crecimiento de Fibroblastos/metabolismo , Células Ciliadas Auditivas/citología , ARN Citoplasmático Pequeño/genética , Receptores Notch/metabolismo , Transducción de Señal , Células Madre/metabolismo , Animales , Pez CebraRESUMEN
Collective cell migration is an essential process during embryonic development and diseases such as cancer, and still much remains to be learned about how cell intrinsic and environmental cues are coordinated to guide cells to their targets. The migration-dependent development of the zebrafish sensory lateral line proves to be an excellent model to study how proteoglycans control collective cell migration in a vertebrate. Proteoglycans are extracellular matrix glycoproteins essential for the control of several signaling pathways including Wnt/ß-catenin, Fgf, BMP and Hh. In the lateral line primordium the modified sugar chains on proteoglycans are important regulators of cell polarity, ligand distribution and Fgf signaling. At least five proteoglycans show distinct expression patterns in the primordium; however, their individual functions have not been studied. Here, we describe the function of glypican4 during zebrafish lateral line development. glypican4 is expressed in neuromasts, interneuromast cells and muscle cells underlying the lateral line. knypekfr6/glypican4 mutants show severe primordium migration defects and the primordium often U-turns and migrates back toward the head. Our analysis shows that Glypican4 regulates the feedback loop between Wnt/ß-catenin/Fgf signaling in the primordium redundantly with other Heparan Sulfate Proteoglycans. In addition, the primordium migration defect is caused non-cell autonomously by the loss of cxcl12a-expressing muscle precursors along the myoseptum via downregulation of Hh. Our results show that glypican4 has distinct functions in primordium cells and cells in the environment and that both of these functions are essential for collective cell migration.
Asunto(s)
Glipicanos/fisiología , Proteoglicanos de Heparán Sulfato/fisiología , Sistema de la Línea Lateral/embriología , Proteínas de Pez Cebra/fisiología , Animales , Proteínas Morfogenéticas Óseas/fisiología , Movimiento Celular , Polaridad Celular , Ectodermo/citología , Ectodermo/fisiología , Ectodermo/trasplante , Retroalimentación Fisiológica , Gástrula/fisiología , Regulación del Desarrollo de la Expresión Génica , Glipicanos/genética , Proteínas Hedgehog/fisiología , Sistema de la Línea Lateral/citología , Desarrollo de Músculos/fisiología , Músculo Esquelético/embriología , Vía de Señalización Wnt/fisiología , Pez Cebra/embriologíaRESUMEN
BACKGROUND: Damage or destruction of sensory hair cells in the inner ear leads to hearing or balance deficits that can be debilitating, especially in older adults. Unfortunately, the damage is permanent, as regeneration of the inner ear sensory epithelia does not occur in mammals. RESULTS: Zebrafish and other non-mammalian vertebrates have the remarkable ability to regenerate sensory hair cells and understanding the molecular and cellular basis for this regenerative ability will hopefully aid us in designing therapies to induce regeneration in mammals. Zebrafish not only possess hair cells in the ear but also in the sensory lateral line system. Hair cells in both organs are functionally analogous to hair cells in the inner ear of mammals. The lateral line is a mechanosensory system found in most aquatic vertebrates that detects water motion and aids in predator avoidance, prey capture, schooling, and mating. Although hair cell regeneration occurs in both the ear and lateral line, most research to date has focused on the lateral line due to its relatively simple structure and accessibility. CONCLUSIONS: Here we review the recent discoveries made during the characterization of hair cell regeneration in zebrafish.
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Oído Interno/citología , Células Ciliadas Auditivas/fisiología , Regeneración/fisiología , Pez Cebra/fisiología , Animales , Animales Modificados Genéticamente , Muerte Celular/genética , Oído Interno/fisiología , Expresión Génica , Sistema de la Línea Lateral/citología , Sistema de la Línea Lateral/fisiología , Regeneración/genéticaRESUMEN
Proper orchestration of quiescence and activation of progenitor cells is crucial during embryonic development and adult homeostasis. We took advantage of the zebrafish sensory lateral line to define niche-progenitor interactions to understand how integration of diverse signaling pathways spatially and temporally regulates the coordination of these processes. Our previous studies demonstrated that Schwann cells play a crucial role in negatively regulating lateral line progenitor proliferation. Here we demonstrate that ErbB/Neuregulin signaling is not only required for Schwann cell migration but that it plays a continued role in postmigratory Schwann cells. ErbB expressing Schwann cells inhibit lateral line progenitor proliferation and differentiation through non-cell-autonomous inhibition of Wnt/ß-catenin signaling. Subsequent activation of Fgf signaling controls sensory organ differentiation, but not progenitor proliferation. In addition to the lateral line, these findings have important implications for understanding how niche-progenitor cells segregate interactions during development, and how they may go wrong in disease states. DOI: http://dx.doi.org/10.7554/eLife.01832.001.
Asunto(s)
Comunicación Celular , Receptores ErbB/metabolismo , Sistema de la Línea Lateral/metabolismo , Células-Madre Neurales/metabolismo , Células de Schwann/metabolismo , Vía de Señalización Wnt , Proteínas de Pez Cebra/metabolismo , beta Catenina/metabolismo , Animales , Animales Modificados Genéticamente , Comunicación Celular/efectos de los fármacos , Diferenciación Celular , Proliferación Celular , Receptores ErbB/antagonistas & inhibidores , Receptores ErbB/genética , Factores de Crecimiento de Fibroblastos/metabolismo , Regulación del Desarrollo de la Expresión Génica , Genotipo , Sistema de la Línea Lateral/citología , Sistema de la Línea Lateral/efectos de los fármacos , Mutación , Células-Madre Neurales/efectos de los fármacos , Neurregulinas/metabolismo , Fenotipo , Inhibidores de Proteínas Quinasas/farmacología , Receptores Notch/metabolismo , Células de Schwann/efectos de los fármacos , Nicho de Células Madre , Factores de Tiempo , Vía de Señalización Wnt/efectos de los fármacos , Pez Cebra/genética , Pez Cebra/metabolismo , Proteínas de Pez Cebra/genética , beta Catenina/genéticaRESUMEN
The mechanisms of hypoxic injury to the developing human brain are poorly understood, despite being a major cause of chronic neurodevelopmental impairments. Recent work in the invertebrate Caenorhabditis elegans has shown that hypoxia causes discrete axon pathfinding errors in certain interneurons and motorneurons. However, it is unknown whether developmental hypoxia would have similar effects in a vertebrate nervous system. We have found that developmental hypoxic injury disrupts pathfinding of forebrain neurons in zebrafish (Danio rerio), leading to errors in which commissural axons fail to cross the midline. The pathfinding defects result from activation of the hypoxia-inducible transcription factor (hif1) pathway and are mimicked by chemical inducers of the hif1 pathway or by expression of constitutively active hif1α. Further, we found that blocking transcriptional activation by hif1α helped prevent the guidance defects. We identified ephrinB2a as a target of hif1 pathway activation, showed that knock-down of ephrinB2a rescued the guidance errors, and showed that the receptor ephA4a is expressed in a pattern complementary to the misrouting axons. By targeting a constitutively active form of ephrinB2a to specific neurons, we found that ephrinB2a mediates the pathfinding errors via a reverse-signaling mechanism. Finally, magnesium sulfate, used to improve neurodevelopmental outcomes in preterm births, protects against pathfinding errors by preventing upregulation of ephrinB2a. These results demonstrate that evolutionarily conserved genetic pathways regulate connectivity changes in the CNS in response to hypoxia, and they support a potential neuroprotective role for magnesium.
Asunto(s)
Efrina-B2/genética , Subunidad alfa del Factor 1 Inducible por Hipoxia , Hipoxia , Sulfato de Magnesio/farmacología , Neuronas/metabolismo , Fármacos Neuroprotectores/farmacología , Pez Cebra , Animales , Animales Modificados Genéticamente , Axones/metabolismo , Axones/fisiología , Sistema Nervioso Central/metabolismo , Efrina-B2/metabolismo , Regulación del Desarrollo de la Expresión Génica , Técnicas de Silenciamiento del Gen , Hipoxia/metabolismo , Hipoxia/patología , Subunidad alfa del Factor 1 Inducible por Hipoxia/genética , Subunidad alfa del Factor 1 Inducible por Hipoxia/metabolismo , Neuronas/patología , Receptor EphA4/genética , Receptor EphA4/metabolismo , Transducción de Señal , Activación Transcripcional , Pez Cebra/genética , Pez Cebra/fisiologíaRESUMEN
Olfactory sensory neurons expressing particular olfactory receptors project to specific reproducible locations within the bulb. The axonal guidance cues that organize this precise projection pattern are only beginning to be identified. To aid in their identification and characterization, we generated a transgenic zebrafish line, OR111-7:IRES:Gal4, in which a small subset of olfactory sensory neurons is labeled. Most sensory neurons expressing the OR111-7 transgene project to a specific location within the bulb, the central zone protoglomerulus, while a smaller number project to the lateral glomerulus 1 protoglomerulus. Inhibiting Netrin/DCC (deleted in colorectal cancer) signaling perturbs the ability of OR111-7-expressing axons to enter the olfactory bulb and alters their patterns of termination within the bulb. The Netrin receptor DCC is expressed in olfactory sensory neurons around the time that they elaborate their axons, netrin1a is expressed near the medial-most margin of the olfactory bulb, and netrin1b is expressed within the ventral region of the bulb. Loss of Netrin/DCC signaling components causes some OR111-7-expressing sensory axons to wander posteriorly after exiting the olfactory pit, away from netrin-expressing areas in the bulb. OR111-7-expressing axons that enter the bulb target the central zone less precisely than normal, spreading away from netrin-expressing regions. These pathfinding errors can be corrected by the reexpression of DCC within OR111-7 transgene-expressing neurons in DCC morphant embryos. These findings implicate Netrins as the only known attractants for olfactory sensory neurons, first drawing OR111-7-expressing axons into the bulb and then into the ventromedially positioned central zone protoglomerulus.
Asunto(s)
Axones/fisiología , Factores de Crecimiento Nervioso/fisiología , Bulbo Olfatorio/anatomía & histología , Receptores de Superficie Celular/fisiología , Transducción de Señal/fisiología , Proteínas Supresoras de Tumor/fisiología , Proteínas de Pez Cebra/fisiología , Animales , Animales Modificados Genéticamente , Axones/efectos de los fármacos , Receptor DCC , Femenino , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Masculino , Imagen Molecular/métodos , Morfolinos/farmacología , Mutación , Factores de Crecimiento Nervioso/metabolismo , Netrina-1 , Bulbo Olfatorio/efectos de los fármacos , Proteína Marcadora Olfativa/genética , Proteína Marcadora Olfativa/metabolismo , Neuronas Receptoras Olfatorias/efectos de los fármacos , Neuronas Receptoras Olfatorias/fisiología , Receptores de Superficie Celular/metabolismo , Receptores Odorantes/genética , Receptores Odorantes/metabolismo , Transducción de Señal/efectos de los fármacos , Canales Catiónicos TRPC/genética , Canales Catiónicos TRPC/metabolismo , Proteínas Supresoras de Tumor/metabolismo , Pez Cebra/genética , Proteínas de Pez Cebra/metabolismoRESUMEN
During peripheral nerve development, each segment of a myelinated axon is matched with a single Schwann cell. Tight regulation of Schwann cell movement, proliferation and differentiation is essential to ensure that these glial cells properly associate with axons. ErbB receptors are required for Schwann cell migration, but the operative ligand and its mechanism of action have remained unknown. We demonstrate that zebrafish Neuregulin 1 (Nrg1) type III, which signals through ErbB receptors, controls Schwann cell migration in addition to its previously known roles in proliferation and myelination. Chimera analyses indicate that ErbB receptors are required in all migrating Schwann cells, and that Nrg1 type III is required in neurons for migration. Surprisingly, expression of the ligand in a few axons is sufficient to induce migration along a chimeric nerve constituted largely of nrg1 type III mutant axons. These studies also reveal a mechanism that allows Schwann cells to fasciculate axons regardless of nrg1 type III expression. Time-lapse imaging of transgenic embryos demonstrated that misexpression of human NRG1 type III results in ectopic Schwann cell migration, allowing them to aberrantly enter the central nervous system. These results demonstrate that Nrg1 type III is an essential signal that controls Schwann cell migration to ensure that these glia are present in the correct numbers and positions in developing nerves.
Asunto(s)
Movimiento Celular/fisiología , Neurregulina-1/metabolismo , Isoformas de Proteínas/metabolismo , Células de Schwann/fisiología , Pez Cebra/anatomía & histología , Secuencia de Aminoácidos , Animales , Animales Modificados Genéticamente , Biomarcadores/metabolismo , Diferenciación Celular/fisiología , Proliferación Celular , Humanos , Datos de Secuencia Molecular , Neurregulina-1/genética , Neuronas/citología , Neuronas/metabolismo , Isoformas de Proteínas/genética , Receptor ErbB-2/genética , Receptor ErbB-2/metabolismo , Células de Schwann/citología , Alineación de Secuencia , Quimera por Trasplante , Pez Cebra/embriologíaRESUMEN
The rodent barrel cortex is a useful system to study the role of genes and neuronal activity in the patterning of the nervous system. Several genes encoding either intracellular signaling molecules or neurotransmitter receptors are required for barrel formation. Neurofibromin is a tumor suppressor protein that has Ras GTPase activity, thus attenuating the MAPK (mitogen-activated protein kinase) and and PI-3 kinase (phosphatidylinositol 3-kinase) pathways, and is mutated in humans with the condition neurofibromatosis type 1 (NF1). Neurofibromin is widely expressed in the developing and adult nervous system, and a common feature of NF1 is deficits in intellectual development. In addition, NF1 is an uncommonly high disorder among individuals with autism. Thus, NF1 may have important roles in normal CNS development and function. To explore roles for neurofibromin in the development of the CNS, we took advantage of a mouse conditional allele. We show that mice that lack neurofibromin in the majority of cortical neurons and astrocytes fail to form cortical barrels in the somatosensory cortex, whereas segregation of thalamic axons within the somatosensory cortex appears unaffected.
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Astrocitos/metabolismo , Neurofibromina 1/metabolismo , Neuronas/metabolismo , Corteza Somatosensorial/citología , Corteza Somatosensorial/metabolismo , Animales , Axones/fisiología , Mapeo Encefálico , Línea Celular , Genes de Neurofibromatosis 1/fisiología , Ratones , Corteza Somatosensorial/crecimiento & desarrollo , Tálamo/crecimiento & desarrolloRESUMEN
Ras-mediated signaling pathways participate in multiple aspects of neural development and function. For example, Ras signaling lies downstream of neurotrophic factors and Trk family receptor tyrosine kinases to regulate neuronal survival and morphological differentiation, including axon extension and target innervation. Neurofibromin, the protein encoded by the tumor suppressor gene Nf1, is a negative regulator of Ras [Ras-GAP (GTPase-activating protein)], and we previously demonstrated that Nf1 null embryonic sensory and sympathetic neurons can survive and differentiate independent of neurotrophin support. In this report, we demonstrate that Nf1 loss in adult sensory neurons enhances their intrinsic capacity for neurite outgrowth and collateral branching in vitro and in vivo after dorsal root injury. In contrast to the permanent sensory deficits observed in control mice after dorsal rhizotomy, neuron-specific Nf1 mutant mice spontaneously recover proprioceptive function. This phenomenon appears to be mediated both by a cell-autonomous capacity of spared Nf1-/- DRG neurons for increased axonal sprouting, and by non-cell-autonomous contribution from Nf1-/- neurons in the denervated spinal cord.
Asunto(s)
Axones/ultraestructura , Ganglios Espinales/lesiones , Ganglios Espinales/fisiopatología , Eliminación de Gen , Neurofibromina 1/genética , Neuronas Aferentes/metabolismo , Animales , Ganglios Espinales/patología , Silenciador del Gen , Integrasas , Ratones , Ratones Noqueados , Neuritas , Neurofibromina 1/deficiencia , Neuronas Aferentes/ultraestructura , Propiocepción , Recuperación de la Función , Rizotomía , Médula Espinal/metabolismo , Médula Espinal/fisiopatologíaRESUMEN
The gene responsible for neurofibromatosis type 1 (NF1) encodes a tumor suppressor that functions as a negative regulator of the Ras proto-oncogene. Individuals with germline mutations in NF1 are predisposed to the development of benign and malignant tumors of the peripheral and central nervous system (CNS). Children with this disease suffer a high incidence of optic gliomas, a benign but potentially debilitating tumor of the optic nerve; and an increased incidence of malignant astrocytoma, reactive astrogliosis and intellectual deficits. In the present study, we have sought insight into the molecular and cellular basis of NF1-associated CNS pathologies. We show that mice genetically engineered to lack NF1 in CNS exhibit a variety of defects in glial cells. Primary among these is a developmental defect resulting in global reactive astrogliosis in the adult brain and increased proliferation of glial progenitor cells leading to enlarged optic nerves. As a consequence, all of the mutant optic nerves develop hyperplastic lesions, some of which progress to optic pathway gliomas. These data point to hyperproliferative glial progenitors as the source of the optic tumors and provide a genetic model for NF1-associated astrogliosis and optic glioma.
Asunto(s)
Encéfalo/metabolismo , Neurofibromina 1/fisiología , Neuroglía/metabolismo , Glioma del Nervio Óptico/metabolismo , Células Madre/fisiología , Animales , Astrocitos/metabolismo , Astrocitos/patología , Encéfalo/crecimiento & desarrollo , Encéfalo/patología , Diferenciación Celular , Linaje de la Célula , Proliferación Celular , Hiperplasia , Ratones , Ratones Noqueados , Mutación , Neurofibromina 1/genética , Neuroglía/patología , Nervio Óptico/patología , Glioma del Nervio Óptico/genética , Glioma del Nervio Óptico/patologíaRESUMEN
The inability of CNS axons to regenerate after traumatic spinal cord injury is due, in part, to the inhibitory effects of myelin. The three major previously identified constituents of this activity (Nogo, myelin-associated glycoprotein, and oligodendrocyte myelin glycoprotein) were isolated based on their potent inhibition of axon outgrowth in vitro. All three myelin components transduce their inhibitory signals through the same Nogo receptor/p75 neurotrophin receptor/LINGO-1 (NgR1/p75/LINGO-1) complex. In this study, we considered that molecules known to act as repellants in vertebrate embryonic axonal pathfinding may also inhibit regeneration. In mice, ephrin-B3 functions during development as a midline repellant for axons of the corticospinal tract. We therefore investigated whether this repellant was expressed in the adult spinal cord and retained inhibitory activity. We demonstrate that ephrin-B3 is expressed in postnatal myelinating oligodendrocytes and, by using primary CNS neurons, show that ephrin-B3 accounts for an inhibitory activity equivalent to that of the other three myelin-based inhibitors, acting through p75, combined. Our data describe a known vertebrate axon guidance molecule as a myelin-based inhibitor of neurite outgrowth.
Asunto(s)
Sistema Nervioso Central/crecimiento & desarrollo , Efrina-B3/metabolismo , Vaina de Mielina/metabolismo , Neuritas/fisiología , Oligodendroglía/fisiología , Animales , Western Blotting , Sistema Nervioso Central/metabolismo , Inmunohistoquímica , Hibridación in Situ , Ratones , Ratones Mutantes , Neuritas/metabolismo , Oligodendroglía/metabolismo , Receptor de Factor de Crecimiento Nervioso/metabolismo , beta-GalactosidasaRESUMEN
In the rodent central nervous system, the region of the cortex that responds to facial whisker stimulation is anatomically segregated into discrete regions called barrels. Each barrel is made up of layer IV cortical neurons that receive input from a separate whisker via innervation from the thalamus. It has been shown that neurotrophins play important roles in the development and plasticity of thalamic axon innervation into the visual and retrosplenial cortex. We now extend those findings to the investigation of the role of neurotrophin signaling in barrel cortex formation. We show that the neurotrophin receptor TrkB is expressed in the thalamus and cortex during the time of cortical innervation. The two TrkB ligands, brain derived neurotrophic factor (BDNF) and neurotrophin-4 (NT-4), are expressed in the cortex at this time. Mice lacking TrkB demonstrate a developmental delay in the segregation of thalamic axons within barrels. In TrkB mutants, thalamic axons are abnormally uniform within layer IV of the cortex at postnatal day 4 compared to their control littermates, but show clear segregation into barrels 2 days later. This phenotype is recapitulated in BDNF mutant mice, but not in NT-4 mutant mice. These results demonstrate that BDNF is the sole TrkB ligand responsible for this phenotype. Analysis of conditional knockout mice that lack TrkB within the cortex, and not the thalamus, does not show a delay in thalamic axon segregation. These results indicate that TrkB expression in thalamic axons is important for the appropriate timing of barrel cortex development.
Asunto(s)
Diferenciación Celular/fisiología , Neuronas/metabolismo , Receptor trkB/metabolismo , Transducción de Señal/fisiología , Corteza Somatosensorial/crecimiento & desarrollo , Corteza Somatosensorial/metabolismo , Vías Aferentes/citología , Vías Aferentes/crecimiento & desarrollo , Vías Aferentes/metabolismo , Envejecimiento/fisiología , Animales , Animales Recién Nacidos , Factor Neurotrófico Derivado del Encéfalo/metabolismo , Regulación del Desarrollo de la Expresión Génica/genética , Conos de Crecimiento/metabolismo , Ratones , Ratones Noqueados , Ratones Mutantes , Ratones Transgénicos , Mutación/genética , Factores de Crecimiento Nervioso/metabolismo , Neuronas/citología , Receptor trkB/genética , Corteza Somatosensorial/anatomía & histología , Nervio Trigémino/crecimiento & desarrollo , Núcleos Talámicos Ventrales/crecimiento & desarrollo , Núcleos Talámicos Ventrales/metabolismo , Vibrisas/inervación , Vibrisas/fisiologíaRESUMEN
Neurotrophin signaling has been implicated in the processes of synapse formation and plasticity. To gain additional insight into the mechanism of BDNF and TrkB influence on synapse formation and synaptic plasticity, we generated a conditional knock-out for TrkB using the cre/loxp system. Using three different cre-expressing transgenic mice, three unique spatial and temporal configurations of TrkB deletion were obtained with regard to the hippocampal Schaffer collateral synapse. We compare synapse formation in mutants in which TrkB is ablated either in presynaptic or in both presynaptic and postsynaptic cells at early developmental or postdevelopmental time points. Our results indicate a requirement for TrkB at both the presynaptic and postsynaptic sites during development. In the absence of TrkB, synapse numbers were significantly reduced. In vivo ablation of TrkB after synapse formation did not affect synapse numbers. In primary hippocampal cultures, deletion of TrkB in only the postsynaptic cell, before synapse formation, also resulted in deficits of synapse formation. We conclude that TrkB signaling has a cell-autonomous role required for normal development of both presynaptic and postsynaptic components of the Schaffer collateral synapse.