[Clinical analysis and countermeasures of 739 cases treated in the emergency department of stomatology in general hospital during COVID-19 epidemic].

PURPOSE: In this paper, based on the age, sex, disease type, and consultation time of dental emergency during COVID-19 epidemic, a comprehensive analysis of dental emergency management and prevention and control of COVID-19 was conducted. METHODS: A total of 739 emergency dental cases were collected from January 29 2020 to February 28 2020. They were divided into 3 groups，including adolescents (≤18 years), young adults (18 ~ 60 years), and elderly ( ≥60 years old). The data was analyzed using SPSS 21.0 software package. RESULTS: The ratio of male to female was 1.24∶1. There were 655 emergency cases during the day and 84 cases during the night. The types of diseases included pericoronitis (15.83%), apical periodontitis (14.21%), pulpitis (13.40%), periodontitis (12.31%), oral mucosal disease (12.18%), Oral and maxillofacial trauma (10.55%), oral and maxillofacial space infection (8.39%), dental disease in children (5.41%), oral and maxillofacial tumors (2.84%), temporomandibular joint dislocations and disorders (1.76%), and others (3.11%). CONCLUSIONS: Under the epidemic situation of the new coronavirus, as one of the high-risk departments, it is of great significance to enhance the clinical emergency skills and ability of emergency treatment, improve patients' oral health awareness, address the diagnosis and treatment of essential diseases, for the improvement the quality of dental medical care and the prevention and control of COVID-19 epidemic.

[Synthetic PTHrP1-36 and its role in promoting alveolar bone formation].

PURPOSE: To synthetise mouse parathyroid hormone related protein 1-36 (PTHrP1-36) and to study its role in promoting alveolar bone formation. METHODS: pGEX-2TK/ PTHrP1-36 was constructed and PTHrP1-36 was expressed. The role of PTHrP1-36 in promoting alveolar bone formation was observed by treating bone marrow cells in vitro and ovariectomy-induced maxillofacial osteoporosis in vivo. Statistical analysis was performed using SPSS 10.0 software package. RESULTS: The results of PCR analysis showed that pGEX-2TK/ PTHrP1-36 plasmid was correct templates for expressing PTHrP1-36. The molecular weights obtained by SDS-PAGE were the same as the theoretical value after IPTG induction. Scale up production was carried out by 3-hour-induction with 1mM IPTG which was the optimal induction factors. Bone marrow cell culture revealed that synthetic PTHrP1-36 increased the number of total fibroblastic colony forming units (CFU-f), ALP positive CFU-f and calcific nodules. After PTHrP1-36 was subcutaneously administered to ovariectomy mice, density and volume of alveolar bone were improved, as showed by X-ray and histochemistry staining of collagen-l and H-E staining. CONCLUSIONS: Synthetic PTHrP1-36 can promote bone marrow cell osteogenic differentiation and increase alveolar bone formation.

[Effects of high temperature on Bt protein content and nitrogen metabolic physiology in boll wall of Bt cotton].

Bt cotton cultivar Sikang 1 (a conventional cultivar) and Sikang 3 (a hybrid cultivar) from China, and 99B (a conventional cultivar) and Daiza 1 (a hybrid cultivar) from USA were selected as experimental materials, the ball wall Bt protein content and nitrogen metabolic physiology were investigated under different high temperature levels at peak boll stage. The results showed that the Bt protein content of boll wall decreased with the increasing temperature. Compared with the control (32 °C, the boll wall Bt protein content decreased significantly when the temperature was above 38 °C for the conventional cultivars and above 40 °C for the hybrid cultivars. The Bt protein contents of cultivar Sikang 1 and 99B decreased by 53.0% and 69.5% respectively with the temperature at 38 °C, and that of cultivar Sikang 3 and Daiza 1 decreased by 64.8% and 54.1% respectively with the temperature at 40 °C. Greater reductions in the boll wall soluble protein contents and GPT activities, larger increments for the boll wall free amino acid contents and proteinsase activities were also observed when the boll wall Bt protein content was significantly reduced. Therefore, high temperature resulted in the reduction of Bt protein synthesis and increase of the insecticidal protein degradation in the boll wall significantly, which caused the reductions in boll wall Bt protein content and insect resistance.