Structures, functions, and inhibitors of LUBAC and its related diseases.

Ubiquitination is a reversible posttranslational modification in which ubiquitin is covalently attached to substrates at catalysis by E1, E2, and E3 enzymes. As the only E3 ligase for assembling linear ubiquitin chains in animals, the LUBAC complex exerts an essential role in the wide variety of cellular activities. Recent advances in the LUBAC complex, including structure, physiology, and correlation with malignant diseases, have enabled the discovery of potent inhibitors to treat immune-related diseases and cancer brought by LUBAC complex dysfunction. In this review, we summarize the current progress on the structures, physiologic functions, inhibitors of LUBAC, and its potential role in immune diseases, tumors, and other diseases, providing the theoretical basis for therapy of related diseases targeting the LUBAC complex.

Comprehensive assessment of three typical antibiotics on cyanobacteria (Microcystis aeruginosa): The impact and recovery capability.

This innovative study provided a comprehensive evaluation of the effects of three typical antibiotics exposures (cefradine, norfloxacin and amoxicillin) on Microcystis aeruginosa in two periods (exposure and post-exposure) at a new perspective. The results indicated that the irreversible growth inhibition of M. aeruginosa attributed to the norfloxacin in the exposure and the re-exposure stages. In contrast, although the algal cell size recovered to the control level after the exposure of 20 mg/L of cefradine, the significant stimulation on glutathione (GSH) still persisted even if the contaminants were removed. On the other hand, amoxicillin inhibited the activities of superoxide dismutase (SOD), GSH contents and the algal cell size in the exposure period while malonaldehyde (MDA) contents increased significantly in two periods.

Occurrence, distribution and sources of bisphenol analogues in a shallow Chinese freshwater lake (Taihu Lake): Implications for ecological and human health risk.

Since the production and use of BPA were regulated in China around 2008, several bisphenol analogues were widely used to substitute BPA in the manufacture of polycarbonates, epoxy resins, and plastics. However, there is limited understanding of the spatial distribution, potential sources and risk assessment for those bisphenol analogues. In this study, seven bisphenol analogues were investigated in water and sediment samples from Taihu Lake, China. Compared to the same lake in 2013 (range: 5.4-87ng/L for waters and 0.37-8.3ng/g dw for sediments), the samples from Taihu Lake contained comparatively higher BPs (2.0×102-9.5×102ng/L and 23-4.3×102ng/gdw) in 2016, indicating that the BPs levels in Taihu Lake have aggravated recently. In waters, BPAF has become the predominant congener in Taihu Lake, suggesting that BPAF was the most widely used substitute of BPA, recently. In sediments, BPA was the most abundant compound. Moderate or strong correlations between some individual BPs indicated that those BPs may have the common sources and/or similar environmental behavior. The strongly positive correlation between ∑BPs and TOC content indicated that TOC content was one of the major factors controlled the distribution of ∑BPs in the sediment. The risk assessment at the sampling sites showed no high eco-toxicity or estrogenic risk in Taihu Lake.

[Survey of blindness and low vision among residents in Shuimogou District, Urumqi, China in 2010].

OBJECTIVE: To estimate the prevalence of visual impairment among adults aged ≥ 40 years, compare the prevalence between Uyghur and Han residents and analyze the causes of visual impairment. METHODS: Population-based cluster sampling survey was conducted among a target population of 5032 persons ≥ 40 years of age from Shuimogou District in Urumqi, Xinjiang Uyghur Autonomous Region. Distance visual acuity (VA) was measured with a Tumbling-E chart at 5 m. Presenting and best corrected VA were recorded. Clinical evaluations were performed. And primary causes of visual impairment was determined for those with VA < 0.3. RESULTS: Among them, there were 4104 responders (Uyghur 2006 persons, Han 2098 persons) with a response rate 81.56%. Prevalence of bilateral blindness was 1.25%/1.86% (Uyghur/Han) (P < 0.05) and bilateral low vision 4.04%/3.29% (Uyghur/Han) (P < 0.05). Primary cause of visual impairment among participants was cataract (cause proportion of 49.23% among Uyghurs and 51.85% among Hans). Visual impairment was correlated with advanced age, males and poor literacy. Prevalences of bilateral blindness and bilateral low vision in age group 40 - 50 yr increased from 0.48% and 1.13% to 3.47% and 10.88% in age group ≥ 70 among Uygur citizens respectively (χ(2) = 22.36, P < 0.05 vs χ(2) = 70.97, P < 0.05). Relative data among the Han citizens varied from 0% and 1.42% to 4.39% and 7.89% (χ(2) = 27.94, P < 0.05 vs χ(2) = 39.99, P < 0.05). CONCLUSION: The most frequent cause of visual impairment in this area is cataract (Uyghur 49.23% vs Han 51.85%) while retinal diseases and glaucoma are important causes as well. Blindness prevention programs targeting the elderly should be expanded, particularly in areas with a limited access and affordability of eye care services.

[Establishment and application of co-transfection screening method for phytoestrogen active constituents].

OBJECTIVE: To establish a highly sensitive screening method for phytoestrogen active constituents and to primarily screen the phytoestrogenic active constituents from the chickpea extractions by the method. METHOD: Human ERalpha cDNA was cloned using MCF-7 total RNA as the template by RT-PCR and then was constructed into a pcDNA3 and named as pERalpha. The cell line MCF-7 was co-transfected with pERalpha and the reporter plasmid pERE-Luc which carrying the estrogen response element (ERE) plus the luciferase reporter gene. The luciferase activity was then assayed. The model was optimized by changing the ratio of two plasmids. The feasibility of the optimized model was further proved by the several known phytoestrogen compounds including fermononetin, biochanin A and genistein, et al. As an application of the model, the phytoestrogen activity of the extracts of the chickpea was assayed. RESULT: The recombinant plasmid (pERalpha) can enhance luciferase activities of pERE-Luc transfected MCF-7 cells. The highest transfection efficiency and luciferase activity were found at the ratio of 10:1 (pERE-Luc: pERalpha), the luciferase activity was improved five times as high as the unique pERE-Luc transfection. The co-transfection screening model also indicated that fermononetin, biochanin A and genistein could induce ERE-driven luciferase activity and ICI 182,780 suppressed the induced transcription. As the application of the model, the results showed that the ethanol (70%) total extraction, the ethyl acetate extraction and the ligarine extraction of the chickpea can induce ERE-driven luciferase activity. Concurrent treatment with ICI 182,780 abolished the induced luciferase activity. CONCLUSION: A phytoestrogen active constituent screening mode have been established based on co-transfection method. It is sensitive to assay the phytoestrogen active constituents and can be applied to screen the active component of phytoestrogens.