[The production of high-yielding strain of rubella virus from wild type virus extracted from a patient in the Western Siberia in 2006].

The study was targeted to investigate the propagation of rubella virus in the cell cultures of various origins and with different cultivation methods. The high-yielding strain of rubella virus was produced. The "spinner-culture" cultivation method was applied and the strain's RNA was detected in 10-8 dilution in real time mode. This strain is supposed to be used in preparation of the standard antigen to implement in the development of immune enzyme test system targeted to the rubella virus specific antibodies.

[Genetic variability of hepatitis B virus isolates among population of Shuryskarsky area of Yamal-Nenets autonomous region].

UNLABELLED: The purpose of this work was to determine occurrence of serological markers of hepatites B and to describe subtypes of a superficial antigen and genotypes of hepatitis B virus (HBV) isolates among indigenous population of Yamal-Nenets Autonomous Region (YNAR), Russia. METHODS: We investigated 657 serum samples from inhabitants of Shuryskarsky area of YNAR. ELISA method was used to define the hepatitis B markers: HBsAg, anti-HBs (total) and anti-HBc (IgG and IgM). The HBsAg-positive samples were PCR-tested for the presence of HBV DNA. Genotyping of isolates was by sequencing of the Pre-Sl/Pre-82/S region of HBV genome and phylogenetic analysis. Definition of HBsAg subtypes was executed by two methods: ELISA with subtype-specific monoclonal antibodies and S-gene nucleotide sequence analysis. RESULTS: The following occurrence of hepatitis B markers was observed: HBsAg - 3.2%, anti-HBs (total) - 36.2%, anti-HBc IgG - 30.3%, anti-HBc IgM - 1.6%. Frequency of carrying even one of the markers in the observed population was 47.5%. HBV DNA was found in 17 HBsAg-positive samples. Pre-SI, Pre-S2 and S regions sequences were determined for all HBV DNA-positive samples. The phylogenetic analysis showed an accessory of all investigated HBV isolates to genotype D. HBsAg subtypes distribution appeared the following: ayw2 - 23.5%, ayw3 - 70.6%, adw2 - 5.9%. Results of definition of the subtype ELISA method and by the analysis of S gene nucleotide sequences have coincided in 10/11 (90.1%) cases. CONCLUSIONS: The indigenous population of Shuryskarsky area of YNAR belongs to groups with average HBV carrying. Absolute domination of genotype D (subtypes ayw2, ayw3 and adw2) was revealed. High percentage of concurrence of HBsAg subtypes detected by the ELISA method and method of the analysis of S gene primary structure (90%) was observed. Sequencing of HBV S-gene is preferable to define HBsAg subtypes.

[Random distribution of the level of growth factors in malignant and revertant clones from murine and human tumors]. / Sluchainoe raspredelenie urovnia ékspressii rostovykh faktorov u zlokachestvennykh i revertantnykh klonov myshinykh i chelovecheskikh opukholei.

It is well known that artificial increase in expression of growth factors and their receptors can lead to tumorigenic transformation of cells. Additionally, multiple data on the increased expression of growth factors in many human and animal tumorigenic cells have been published. Nevertheless description of the functional role of endogenous growth factors in maintenance of tumorigenic phenotype remains obscure. Previously, we described a new model for studying neoplastic transformation and dormant metastasis. This model consists of cognate tumorigenic and nontumorigenic cell clones, the latter being obtained as a result of spontaneous reversion of tumorigenic ones. All revertant clones demonstrate the three well known features of normal cells: monolayer growth on a plastic, incapability to grow in soft agar with regular culture media with 10% FCS, and incapability to form tumors in syngeneic animals. Using RT-PCR, we measured expression ratios of main growth factors, commonly believed to be associated with malignization, in tumorigenic and revertant clones of our model. For some of these clones, we also measured an activity of growth factors in conditioned media. The data obtained argue that the levels of growth factor expression, measured by both the methods, are distributed between tumorigenic and revertant clones in a sporadic manner and do not correlate with cell tumorigenicity. Thus, our experimental observations enable us to consider the variability of growth factor expression as insignificant events in the reversion of tumorigenic cells to a nontumorigenic phenotype.