RESUMEN
Using a panel of hybrid clones (common shrew--Chinese hamster and common shrew--mouse), the syntheny and localization of the following genes was determined: genes for alpha-galactosidase (GLA), acid phosphatase (ACP1), and phosphoglycerate kinase (PGK1) on chromosome de; adenosine kinase (ADK) and glucuronidase 2 (GUS2) on chromosome ik; glutamic-oxaloacetic transaminase 2 (GOT2) and peptidase D (PEPD) on chromosome hn; and glyoxalase 1 (GLO1) and phosphoglucomutase 2 (PGM2) on chromosome go. Gene for beta-galactosidase (GLB1) was assigned to arm p of chromosome mp. Thus, including previously mapped genes, the cytogenetic map of the common shrew contains 39 genes. They form seven syntheny groups and mark eight out of ten chromosomes.
Asunto(s)
Mapeo Cromosómico , Cricetulus/genética , Ratones/genética , Musarañas/genética , Animales , Segregación Cromosómica , Clonación Molecular , Cricetinae , Enzimas/genética , Homología de Secuencia de Ácido NucleicoRESUMEN
Syntheny and localization of the following genes in common shrew Sorex araneus were determined: isocitrate dehydrogenase 2 (IDH2), acid phosphatase 2 (ACP2), glutamine--pyruvate--oxo-acid transaminase (GPT), and inorganic pyrophosphatase (PP) on chromosome ik; adenylate kinases 1 and 3 (AK1 and AK3) on chromosome af; and enolase 1 (ENO1) on chromosome jl. Two genes were assigned to definite arms: aminoacylase 1 (ACY1) to arm p of chromosome mp and glutamic-oxaloacetic transaminase 1 (GOT1) to arm q of chromosome qr. Thus, 26 genes marking eight out of ten chromosomes are present now on the cytogenetic map of common shrew. These include previously described localizations.
Asunto(s)
Fosfatasa Ácida/genética , Alanina Transaminasa/genética , Mapeo Cromosómico , Isocitrato Deshidrogenasa/genética , Musarañas/genética , Adenilato Quinasa/genética , Animales , Línea Celular , Marcadores Genéticos , Pirofosfatasa Inorgánica , Cariotipificación , Fosfopiruvato Hidratasa/genética , Pirofosfatasas/genéticaRESUMEN
The comparative cytogenetic analysis of the interspecific mouse-mink hybridoma cells revealed a segregation of the great number of the mink chromosomes, inter- and intraline variability according to the number of cells with the mink DNA and its quantity in the cells. No characteristics of the mink chromosomal material distribution in the hybridoma cells which secreted the immunoglobulins of the American mink or lost its secretion were found. The great changes in the karyotype of the hybrid cells were revealed by in situ hybridization with 3H-labelled total mink DNA. Numerous insertions of the regions from the mink chromosomes to the mouse chromosomes and the appearance of the chromosomes not typical of the mink and mouse parent cells were observed. The number of cells with translocations of fragments from the chromosomes to the mouse one was observed to grow in the hybridoma cell lines cultivated for a long time. Synthesis of the lambda-L-chains of the mink immunoglobulin in the cells of line 7 was absent because they lost lambda-gene.
Asunto(s)
Hibridomas/fisiología , Ratones/genética , Visón/genética , Animales , ADN/análisis , Hibridomas/citología , Inmunoglobulinas/fisiología , Cariotipificación , Translocación Genética/genéticaRESUMEN
We have demonstrated that X chromosomes are reactivated in hybrids obtained by fusion of mouse PCC4azaI teratocarcinoma cells (XO, 39HPRT-) with splenocytes from mouse females heterozygous in Hprt gene. These hybrids are capable of spontaneous differentiation. We also obtained similar interspecies hybrids of PCC4azaI cells with bone marrow cells of the American mink. The majority of such hybrids remained undifferentiated, however, after long-term cultivation at high cell density they differentiated into epithelial- or fibroblast-like cells similarly to PCC4azaI cells. Two hybrids had the autosomal complement of the mouse and two X chromosomes (mouse and mink); both X chromosomes were active. These X chromosomes were not inactivated during differentiation in vitro.