Asunto(s)
Investigación Biomédica/economía , Investigación Biomédica/organización & administración , Redes Comunitarias/organización & administración , Bélgica , Investigación Biomédica/instrumentación , Tecnología Biomédica/economía , Tecnología Biomédica/instrumentación , Tecnología Biomédica/métodos , Tecnología Biomédica/organización & administración , Movilidad Laboral , Redes Comunitarias/economía , Genómica/economía , Genómica/instrumentación , Genómica/organización & administración , Humanos , Laboratorios/economía , Laboratorios/organización & administración , Personal de Laboratorio/economía , Personal de Laboratorio/organización & administración , Proteómica/economía , Proteómica/instrumentación , Proteómica/organización & administración , EspañaRESUMEN
Although it is well described in model membranes, little is known about phase separation in biological membranes. Here, we provide evidence for a coexistence of at least two different lipid bilayer phases in the apical plasma membrane of epithelial cells. Phase connectivity was assessed by measuring long-range diffusion of several membrane proteins by fluorescence recovery after photobleaching in two polarized epithelial cell lines and one fibroblast cell line. In contrast to the fibroblast plasma membrane, in which all of the proteins diffused with similar characteristics, in the apical membrane of epithelial cells the proteins could be divided into two groups according to their diffusion characteristics. At room temperature ( approximately 25 degrees C), one group showed fast diffusion and complete recovery. The other diffused three to four times slower and, more importantly, displayed only partial recovery. Only the first group comprises proteins that are believed to be associated with lipid rafts. The partial recovery is not caused by topological constraints (microvilli, etc.), cytoskeletal constraints, or protein-protein interactions, because all proteins show 100% recovery in fluorescence recovery after photobleaching experiments at 37 degrees C. In addition, the raft-associated proteins cannot be coclustered by antibodies on the apical membrane at 12 degrees C. The interpretation that best fits these data is that the apical membrane of epithelial cells is a phase-separated system with a continuous (percolating) raft phase <25 degrees C in which isolated domains of the nonraft phase are dispersed, whereas at 37 degrees C the nonraft phase becomes the continuous phase with isolated domains of the raft phase dispersed in it.
Asunto(s)
Membrana Celular/química , Membrana Celular/metabolismo , Polaridad Celular , Células Epiteliales/citología , Animales , Anticuerpos/inmunología , Línea Celular , Membrana Celular/inmunología , Difusión , Perros , Recuperación de Fluorescencia tras Fotoblanqueo , Humanos , Cinética , Espectrometría de Fluorescencia , TemperaturaAsunto(s)
Membrana Celular/metabolismo , Aparato de Golgi/metabolismo , Proteínas Proto-Oncogénicas p21(ras)/metabolismo , Acilación , Animales , Diferenciación Celular , Proliferación Celular , Transformación Celular Neoplásica , Células Cultivadas , Citosol/metabolismo , Perros , Transferencia Resonante de Energía de Fluorescencia , Genes ras , Guanosina Trifosfato/metabolismo , Humanos , Sistema de Señalización de MAP Quinasas , Proteínas Quinasas Activadas por Mitógenos/metabolismo , Modelos Biológicos , Ácido Palmítico/metabolismo , Isoformas de Proteínas/química , Isoformas de Proteínas/metabolismo , Proteínas Proto-Oncogénicas p21(ras)/química , Proteínas rasRESUMEN
Epithelial polarization involves the segregation of apical and basolateral membrane domains, which are stabilized and maintained by tight junctions and membrane traffic. We report that unlike most apical and basolateral proteins in MDCK cells, which separate only after junctions have formed, the apical marker gp135 signifies an early level of polarized membrane organization established already in single cells. We identified gp135 as the dog orthologue of podocalyxin. With a series of domain mutants we show that the COOH-terminal PSD-95/Dlg/ZO-1 (PDZ)-binding motif is targeting podocalyxin to the free surface of single cells as well as to a subdomain of the terminally polarized apical membrane. This special localization of podocalyxin is shared by the cytoplasmic PDZ-protein Na+/H+ exchanger regulatory factor (NHERF)-2. Depleting podocalyxin by RNA interference caused defects in epithelial polarization. Together, our data suggest that podocalyxin and NHERF-2 function in epithelial polarization by contributing to an early apical scaffold based on PDZ domain-mediated interactions.
Asunto(s)
Polaridad Celular/fisiología , Proteínas del Citoesqueleto/fisiología , Sialoglicoproteínas/fisiología , Fosfatasa Alcalina , Animales , Cadherinas/metabolismo , Línea Celular , Polaridad Celular/genética , Proliferación Celular , Proteínas del Citoesqueleto/genética , Proteínas del Citoesqueleto/metabolismo , ADN Complementario/genética , Perros , Células Epiteliales/citología , Células Epiteliales/metabolismo , Células Epiteliales/fisiología , Proteínas Ligadas a GPI , Expresión Génica/genética , Glicoproteínas/metabolismo , Glicosilación , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Inmunoprecipitación , Integrina beta1/metabolismo , Isoenzimas/genética , Isoenzimas/metabolismo , Proteínas de la Membrana/metabolismo , Microscopía Fluorescente , Mutación , Plásmidos/genética , Unión Proteica , Interferencia de ARN , Sialoglicoproteínas/química , Sialoglicoproteínas/genética , TransfecciónRESUMEN
We have investigated whether raft lipids of Madin-Darby canine kidney (MDCK) cells play any role in microvilli maintenance using a combination of atomic force microscopy (AFM) and laser scanning confocal microscopy. MDCK cells were treated to reduce the amount of sphingolipids, cholesterol, or both and subsequently imaged, in buffer solution, using AFM. It was observed that inhibition of either sphingolipid or cholesterol biosynthesis led to a reduction in the number of microvilli on the surface of MDCK cells. However, this effect was not uniform across the monolayer, with some cells resembling those in untreated controls. The subsequent extraction of cholesterol from cells grown in the presence of inhibitors led to a further reduction in microvilli on the surface of the cells and, in some cases, resulted in monolayers devoid of full length microvilli. Significantly, smaller spikes were observed on the surface of the smoother cells.