RESUMEN
The technological development of flow cytometry (FC) together with new findings reveal the need for immunophenotyping in research of monoclonal gammopathy (MG) because of its diagnostic, prognostic and predictive significance. The aim of the European Myeloma Network (EMN) is to standardize this analytical method and implement it into routine clinical examination. Since the overall significance and application of FC are still analysed, standardisation could help obtain more clinical relevant information in terms of MG pathophysiology.
Asunto(s)
Citometría de Flujo , Paraproteinemias/inmunología , Células Plasmáticas/inmunología , Antígenos CD/análisis , Citometría de Flujo/métodos , Citometría de Flujo/normas , Humanos , Inmunofenotipificación , Paraproteinemias/patología , Células Plasmáticas/clasificaciónRESUMEN
Multiple myeloma (MM) is a plasma cell (PC) disorder and associated with immune impairments. Flow cytometry based phenotyping and quantification of regulatory T cells (Tregs) enable to monitor the immune status of myeloma patients. Apart from enumeration of Tregs, functional characterization using proliferation or suppression assay adds key value in demonstrating the functional value ofTregs. Our study revealed that in MM patientsTregs are elevated compared to healthy subjects, which demonstrate the immune deregulation in MM.
Asunto(s)
Citometría de Flujo , Inmunofenotipificación , Mieloma Múltiple/inmunología , Linfocitos T Reguladores/inmunología , Humanos , Mieloma Múltiple/terapia , Linfocitos T Reguladores/clasificaciónRESUMEN
Centrosome amplification (CA) as a potential marker of mitotic disruptions in multiple myeloma (MM) was investigated in two populations of B-cell lineage: B-cells and plasma cells (PCs). Using immunofluorescent staining, it was shown that CA in B-cells is present in 3.2+/-2.5% in healthy donors versus 9.9+/-7.9% in MM patients (p<0.0001). Based on the calculated threshold value of CA in B-cells, 37% (14/38) of MM patients were positive. There was no significant correlation between CA-positive MM cases (based on PC samples evaluation) and the occurrence of cytogenetic abnormalities in PCs, including del(13)(q14), del(17)(p13), gain(1)(q21) and hyperdiploidy.