Influence of 40 Hz and 100 Hz Vibration on SH-SY5Y Cells Growth and Differentiation-A Preliminary Study.

(1) Background: A novel bioreactor platform of neuronal cell cultures using low-magnitude, low-frequency (LMLF) vibrational stimulation was designed to discover vibration influence and mimic the dynamic environment of the in vivo state. To better understand the impact of 40 Hz and 100 Hz vibration on cell differentiation, we join biotechnology and advanced medical technology to design the nano-vibration system. The influence of vibration on the development of nervous tissue on the selected cell line SH-SY5Y (experimental research model in Alzheimer's and Parkinson's) was investigated. (2) Methods: The vibration stimulation of cell differentiation and elongation of their neuritis were monitored. We measured how vibrations affect the morphology and differentiation of nerve cells in vitro. (3) Results: The highest average length of neurites was observed in response to the 40 Hz vibration on the collagen surface in the differentiating medium, but cells response did not increase with vibration frequency. Also, vibrations at a frequency of 40 Hz or 100 Hz did not affect the average density of neurites. 100 Hz vibration increased the neurites density significantly with time for cultures on collagen and non-collagen surfaces. The exposure of neuronal cells to 40 Hz and 100 Hz vibration enhanced cell differentiation. The 40 Hz vibration has the best impact on neuronal-like cell growth and differentiation. (4) Conclusions: The data demonstrated that exposure to neuronal cells to 40 Hz and 100 Hz vibration enhanced cell differentiation and proliferation. This positive impact of vibration can be used in tissue engineering and regenerative medicine. It is planned to optimize the processes and study its molecular mechanisms concerning carrying out the research.

Autonomous Instrumentation for Measuring Electromagnetic Radiation from Rocks in Mine Conditions-A Functional Analysis.

This paper analyses the function of an innovative integrated receiver for the measurement of electromagnetic field emissions. The autonomous receiver measures and registers the elevated emission levels of both components of the EM field originating from rocks subjected to increased mechanical stress. The receiver's sensitivity of 60 µV/m, its dynamic range of 98 dB, and its impulse response of 0.23 V/µs were determined in laboratory conditions. Real EM field signals from hard coal samples subjected to crushing force were recorded using an autonomous receiver. The observed and recorded results confirm that the receiver operates in the full range of amplitudes of the EM field signal emitted from the rock. The results determine the band of characteristic signals for EM field emission from hard coal. The system created on the basis of autonomous EM receivers can support the existing seismic safety systems in real mine conditions by predicting the possibility of mine collapse hazards.