RESUMEN
The ascomycetous fungus Nectria haematococca, (asexual name Fusarium solani), is a member of a group of >50 species known as the "Fusarium solani species complex". Members of this complex have diverse biological properties including the ability to cause disease on >100 genera of plants and opportunistic infections in humans. The current research analyzed the most extensively studied member of this complex, N. haematococca mating population VI (MPVI). Several genes controlling the ability of individual isolates of this species to colonize specific habitats are located on supernumerary chromosomes. Optical mapping revealed that the sequenced isolate has 17 chromosomes ranging from 530 kb to 6.52 Mb and that the physical size of the genome, 54.43 Mb, and the number of predicted genes, 15,707, are among the largest reported for ascomycetes. Two classes of genes have contributed to gene expansion: specific genes that are not found in other fungi including its closest sequenced relative, Fusarium graminearum; and genes that commonly occur as single copies in other fungi but are present as multiple copies in N. haematococca MPVI. Some of these additional genes appear to have resulted from gene duplication events, while others may have been acquired through horizontal gene transfer. The supernumerary nature of three chromosomes, 14, 15, and 17, was confirmed by their absence in pulsed field gel electrophoresis experiments of some isolates and by demonstrating that these isolates lacked chromosome-specific sequences found on the ends of these chromosomes. These supernumerary chromosomes contain more repeat sequences, are enriched in unique and duplicated genes, and have a lower G+C content in comparison to the other chromosomes. Although the origin(s) of the extra genes and the supernumerary chromosomes is not known, the gene expansion and its large genome size are consistent with this species' diverse range of habitats. Furthermore, the presence of unique genes on supernumerary chromosomes might account for individual isolates having different environmental niches.
Asunto(s)
Cromosomas Fúngicos/genética , Genoma Fúngico , Nectria/genética , Composición de Base , Cromosomas Fúngicos/química , Hongos/clasificación , Hongos/genética , Duplicación de Gen , Nectria/química , Nectria/clasificación , FilogeniaRESUMEN
Picoeukaryotes are a taxonomically diverse group of organisms less than 2 micrometers in diameter. Photosynthetic marine picoeukaryotes in the genus Micromonas thrive in ecosystems ranging from tropical to polar and could serve as sentinel organisms for biogeochemical fluxes of modern oceans during climate change. These broadly distributed primary producers belong to an anciently diverged sister clade to land plants. Although Micromonas isolates have high 18S ribosomal RNA gene identity, we found that genomes from two isolates shared only 90% of their predicted genes. Their independent evolutionary paths were emphasized by distinct riboswitch arrangements as well as the discovery of intronic repeat elements in one isolate, and in metagenomic data, but not in other genomes. Divergence appears to have been facilitated by selection and acquisition processes that actively shape the repertoire of genes that are mutually exclusive between the two isolates differently than the core genes. Analyses of the Micromonas genomes offer valuable insights into ecological differentiation and the dynamic nature of early plant evolution.
Asunto(s)
Evolución Biológica , Chlorophyta/genética , Genoma , Plantas/genética , Adaptación Fisiológica , Chlorophyta/clasificación , Chlorophyta/citología , Chlorophyta/fisiología , Elementos Transponibles de ADN , Ecosistema , Regulación de la Expresión Génica , Genes , Variación Genética , Intrones , Meiosis/genética , Datos de Secuencia Molecular , Océanos y Mares , Fotosíntesis/genética , Filogenia , Fitoplancton/clasificación , Fitoplancton/genética , ARN no Traducido , Secuencias Repetitivas de Ácidos Nucleicos , Análisis de Secuencia de ADN , Factores de Transcripción/genéticaRESUMEN
The ChromDB website (http://www.chromdb.org) displays chromatin-associated proteins, including RNAi-associated proteins, for a broad range of organisms. Our primary focus is to display sets of highly curated plant genes predicted to encode proteins associated with chromatin remodeling. Our intent is to make this intensively curated sequence information available to the research and teaching communities in support of comparative analyses toward understanding the chromatin proteome in plants, especially in important crop species such as corn and rice. Model animal and fungal proteins are included in the database to facilitate a complete, comparative analysis of the chromatin proteome and to make the database applicable to all chromatin researchers and educators. Chromatin biology and chromatin remodeling are complex processes involving a multitude of proteins that regulate the dynamic changes in chromatin structure which either repress or activate transcription. We strive to organize ChromDB data in a straightforward and comparative manner to help users understand the complement of proteins involved in packaging DNA into chromatin.
Asunto(s)
Cromatina/química , Proteínas Cromosómicas no Histona/química , Bases de Datos de Proteínas , Histonas/química , Proteínas de Plantas/química , Animales , Ensamble y Desensamble de Cromatina , Proteínas Cromosómicas no Histona/genética , Proteínas Fúngicas/química , Genoma de Planta , Internet , Proteínas de Plantas/genética , Análisis de Secuencia de Proteína , Interfaz Usuario-ComputadorRESUMEN
The smallest known eukaryotes, at approximately 1-mum diameter, are Ostreococcus tauri and related species of marine phytoplankton. The genome of Ostreococcus lucimarinus has been completed and compared with that of O. tauri. This comparison reveals surprising differences across orthologous chromosomes in the two species from highly syntenic chromosomes in most cases to chromosomes with almost no similarity. Species divergence in these phytoplankton is occurring through multiple mechanisms acting differently on different chromosomes and likely including acquisition of new genes through horizontal gene transfer. We speculate that this latter process may be involved in altering the cell-surface characteristics of each species. In addition, the genome of O. lucimarinus provides insights into the unique metal metabolism of these organisms, which are predicted to have a large number of selenocysteine-containing proteins. Selenoenzymes are more catalytically active than similar enzymes lacking selenium, and thus the cell may require less of that protein. As reported here, selenoenzymes, novel fusion proteins, and loss of some major protein families including ones associated with chromatin are likely important adaptations for achieving a small cell size.
Asunto(s)
Chlorophyta/genética , Células Eucariotas/clasificación , Células Eucariotas/metabolismo , Genoma/genética , Plancton/clasificación , Plancton/genética , Adaptación Fisiológica , Evolución Biológica , Núcleo Celular/genética , Chlorophyta/metabolismo , Cromosomas/genética , Ambiente , Transferencia de Gen Horizontal , Metales/metabolismo , Datos de Secuencia Molecular , Plancton/metabolismo , Selenoproteínas/metabolismo , Vitaminas/metabolismoRESUMEN
Control of branch development is a major determinant of architecture in plants. Branching in petunia (Petunia hybrida) is controlled by the DECREASED APICAL DOMINANCE (DAD) genes. Gene functions were investigated by plant grafting, morphology studies, double-mutant characterization, and gene expression analysis. Both dad1-1 and dad3 increased branching mutants can be reverted to a near-wild-type phenotype by grafting to a wild-type or a dad2 mutant root stock, indicating that both genes affect the production of a graft-transmissible substance that controls branching. Expression of the DAD1 gene in the stems of grafted plants, detected by quantitative reverse transcription-polymerase chain reaction correlates with the branching phenotype of the plants. The dad2-1 mutant cannot be reverted by grafting, indicating that this gene acts predominantly in the shoot of the plant. Double-mutant analysis indicates that the DAD2 gene acts in the same pathway as the DAD1 and DAD3 genes because the dad1-1dad2-1 and dad2-1dad3 double mutants are indistinguishable from the dad2-1 mutant. However, the dad1-1dad3 double mutant has an additive phenotype, with decreased height of the plants, delayed flowering, and reduced germination rates compared to the single mutants. This result, together with the observation that the dad1-1 and dad3 mutants cannot be reverted by grafting to each other, suggests that the DAD1 and DAD3 genes act in the same pathway, but not in a simple stepwise fashion.
Asunto(s)
Genes de Plantas/genética , Petunia/crecimiento & desarrollo , Petunia/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Tallos de la Planta/crecimiento & desarrollo , Regulación de la Expresión Génica de las Plantas , Mutación , Petunia/metabolismo , Tallos de la Planta/genética , Tallos de la Planta/metabolismoRESUMEN
RNA interference (RNAi) can be used to study gene function by effecting degradation of the targeted transcript. However, the effectiveness of transgene-induced RNAi among multiple target genes has not been compared systematically. To this end, we developed a relative quantitative RT-PCR protocol that allows use of a single internal standard over a wide range of target gene expression levels. Using this method in an analysis of transgenic Arabidopsis thaliana RNAi lines targeting 25 different endogenes revealed that independent, homozygous, single-copy (sc) T4 lines targeting the same gene generally reduce transcript levels to the same extent, whereas multi-copy RNAi lines differed in the degree of target reduction and never exceeded the effect of sc transgenes. The maximal reduction of target transcript levels varied among targets. These observations suggest that each target sequence possesses an inherent degree of susceptibility to dsRNA-mediated degradation.
Asunto(s)
Plantas Modificadas Genéticamente/genética , Interferencia de ARN , Transcripción Genética/genética , Arabidopsis/genética , Secuencia de Bases , ADN Complementario/genética , Amplificación de Genes/genética , Genes de Plantas/genética , Homocigoto , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Moldes Genéticos , TransgenesRESUMEN
Histone proteins play a central role in chromatin packaging, and modification of histones is associated with chromatin accessibility. SET domain [Su(var)3-9, Enhancer-of-zeste, Trithorax] proteins are one class of proteins that have been implicated in regulating gene expression through histone methylation. The relationships of 22 SET domain proteins from maize (Zea mays) and 32 SET domain proteins from Arabidopsis were evaluated by phylogenetic analysis and domain organization. Our analysis reveals five classes of SET domain proteins in plants that can be further divided into 19 orthology groups. In some cases, such as the Enhancer of zeste-like and trithorax-like proteins, plants and animals contain homologous proteins with a similar organization of domains outside of the SET domain. However, a majority of plant SET domain proteins do not have an animal homolog with similar domain organization, suggesting that plants have unique mechanisms to establish and maintain chromatin states. Although the domains present in plant and animal SET domain proteins often differ, the domains found in the plant proteins have been generally implicated in protein-protein interactions, indicating that most SET domain proteins operate in complexes. Combined analysis of the maize and Arabidopsis SET domain proteins reveals that duplication of SET domain proteins in plants is extensive and has occurred via multiple mechanisms that preceded the divergence of monocots and dicots.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Duplicación de Gen , Regulación de la Expresión Génica de las Plantas/fisiología , N-Metiltransferasa de Histona-Lisina , Metiltransferasas/genética , Proteínas de Plantas/genética , Transcripción Genética , Zea mays/genética , Secuencia de Aminoácidos , Animales , Arabidopsis/clasificación , Proteínas de Arabidopsis/química , Secuencia de Bases , Análisis por Conglomerados , Cartilla de ADN , Histona Metiltransferasas , Metiltransferasas/química , Datos de Secuencia Molecular , Filogenia , Proteínas de Plantas/química , Proteína Metiltransferasas , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Zea mays/clasificaciónRESUMEN
The monopodial shoot axis of petunia (Petunia hybrida Vilm) has two different patterns of branch development. Basal lateral branching develops acropetally and is limited to a discrete number of nodes that correlate with the late rosette phase of growth (Zone II). Two zones of suppressed buds immediately precede and follow this zone of branching. Apical branching occurs in response to flowering, develops in a basipetal direction, and is restricted to the distal-most nodes on the monopodial axis. When grown under a short-day regime, an extension to the basal branching zone occurs, and growth of the main shoot axis is retarded. The sym1 mutant has an overall decrease in basal lateral branching compared with wild type whereas the three dad mutants have increased basal branching. The dad1-1 and dad2-1 mutants have no initial zone of suppressed branching whereas the dad3 mutant has a similar Zone II to wild type, but with a greater potential to form branches within this zone. The dad1-1 mutant exhibits delayed flowering, but the dad1-1 sym1 double mutant flowers at a similar node number to wild-type and branching is similar to dad1-1 indicating that these two aspects of the mutant dad1-1 phenotype are independent.
RESUMEN
Sequence similarity and profile searching tools were used to analyze the genome sequences of Arabidopsis thaliana, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans and Drosophila melanogaster for genes encoding three families of histone deacetylase (HDAC) proteins and three families of histone acetyltransferase (HAT) proteins. Plants, animals and fungi were found to have a single member of each of three subfamilies of the GNAT family of HATs, suggesting conservation of these functions. However, major differences were found with respect to sizes of gene families and multi-domain protein structures within other families of HATs and HDACs, indicating substantial evolutionary diversification. Phylogenetic analysis identified a new class of HDACs within the RPD3/HDA1 family that is represented only in plants and animals. A similar analysis of the plant-specific HD2 family of HDACs suggests a duplication event early in dicot evolution, followed by further diversification in the lineage leading to Arabidopsis. Of three major classes of SIR2-type HDACs that are found in animals, fungi have representatives only in one class, whereas plants have representatives only in the other two. Plants possess five CREB-binding protein (CBP)-type HATs compared with one to two in animals and none in fungi. Domain and phylogenetic analyses of the CBP family proteins showed that this family has evolved three distinct types of CBPs in plants. The domain architecture of CBP and TAF(II)250 families of HATs show significant differences between plants and animals, most notably with respect to bromodomain occurrence and their number. Bromodomain-containing proteins in Arabidopsis differ strikingly from animal bromodomain proteins with respect to the numbers of bromodomains and the other types of domains that are present. The substantial diversification of HATs and HDACs that has occurred since the divergence of plants, animals and fungi suggests a surprising degree of evolutionary plasticity and functional diversification in these core chromatin components.
Asunto(s)
Acetiltransferasas/genética , Arabidopsis/enzimología , Arabidopsis/genética , Histona Desacetilasas/genética , Filogenia , Proteínas Represoras , Proteínas de Saccharomyces cerevisiae/genética , Factores de Transcripción , Acetiltransferasas/clasificación , Acetiltransferasas/fisiología , Empalme Alternativo , Secuencia de Aminoácidos , Animales , Proteína de Unión a CREB , Caenorhabditis elegans/enzimología , Caenorhabditis elegans/genética , Cromatina/metabolismo , Proteínas de Drosophila , Drosophila melanogaster/enzimología , Drosophila melanogaster/genética , Proteínas Fúngicas/genética , Genoma de Planta , Histona Acetiltransferasas , Histona Desacetilasa 1 , Histona Desacetilasas/clasificación , Histona Desacetilasas/fisiología , Datos de Secuencia Molecular , Proteínas Nucleares/genética , Estructura Terciaria de Proteína , Saccharomyces cerevisiae/enzimología , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/clasificación , Proteínas de Saccharomyces cerevisiae/fisiología , Schizosaccharomyces/enzimología , Schizosaccharomyces/genética , Homología de Secuencia de Aminoácido , Sirtuinas/genética , Transactivadores/genética , Factores de Transcripción TFII/genéticaRESUMEN
Transgenes designed to overexpress anthocyanin genes An6 (encoding dihydroflavonol-4-reductase) or Hf1 (encoding flavonoid-3',5'-hydroxylase) in Petunia hybrida L. produced flower colour phenotypes similar to those caused by sense cosuppression of chalcone synthase (Chs) genes. However, unlike Chs, sense cosuppression of An6 and Hf1 resulted in female infertility in transgenotes exhibiting complete phenotypic suppression of anthocyanins. Female sterility appeared to be due to embryo abortion, with discolouration of ovules first appearing about 4 d post-fertilization, followed by gradual collapse of the ovule. Pollen from cosuppressed, female-sterile transgenotes placed on wild-type stigmas produced normal seed set, indicating that sterility of cosuppressed plants was maternally controlled. We suggest an hypothesis that cosuppression of An6 and Hf1 leads to accumulation of dihydroflavonols in the seed coat, a maternal tissue, and that this accumulation inhibits embryo growth, either directly or indirectly. In this hypothesis, direct inhibition of embryo growth would require that dihydroflavonols diffuse from the seed coat into the embryo and act there, whereas indirect inhibition would require that dihydroflavonols interfere with some capacity of the seed coat to promote embryo growth.