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Per- and polyfluoroalkyl substances (PFAS) are found in a variety of places including cosmetics, rain jackets, dust, and water. PFAS have also been applied to occupational gear to protect against water and oils. However, PFAS have been identified as immunosuppressants and perfluorooctanoic acid (PFOA), a specific PFAS, has been identified as carcinogenic. Since there is a risk for dermal exposure to these compounds, there is a need to characterize their dermal absorption. Using in vitro flow-through diffusion, skin permeabilities were determined for 14C-labeled perfluorooctanoic acid (PFOA), perfluorohexanoic acid (PFHxA), and perfluorobutanoic acid (PFBA) using porcine skin. Tests were conducted over 8 h with either acetone or artificial perspirant as the vehicle. PFBA was found to have greater permeability than PFHxA, likely due to having a smaller molecular weight. The dosing vehicle did not appear to impact permeability rates but impacted the disposition through the skin model. While these PFAS compounds showed a low permeability rate through the skin membranes, they can stay in the skin, acting as a reservoir.
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OBJECTIVE: To determine the feasibility of catheterizing the portal vein to obtain serial portal vein blood samples in steers. We hypothesized that the portal vein catheterization would be a successful continuous sampling technique with minimal adverse effects in steers. METHODS: 2 groups of steers were used: a pilot group (n = 2) and experimental group (n = 6). In both groups, steers were sedated with xylazine. The right rib spaces were clipped and aseptically prepped. The portal vein was visualized via ultrasound, and a 14-gauge catheter was placed percutaneously and advanced into the portal vein. A guide wire was passed through the catheter, followed by a tissue dilator and then a vascular balloon catheter. In the pilot group, blood chemistries were performed prior to portal vein catheterization and then again once the catheter was placed (with samples from both the jugular vein and portal catheter). The liver was also examined at necropsy for any gross lesions in both groups. RESULTS: All steers tolerated the portal vein catheters well, with the catheters lasting for the full length of the study period (7 days). The only observed adverse reaction was a superficial abscess at the catheter site (n = 3). On necropsy, 1 liver had gross discoloration, but no other abnormalities were noted. There were no significant changes in biochemistry profiles before or after portal vein catheterization. CONCLUSIONS: Portal vein catheterization is a novel and feasible serial sampling technique of the portal vein. CLINICAL RELEVANCE: This technique can be used in future pharmacokinetic, nutrition, metabolism, or toxicity studies.
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The solid-state properties of supramolecular polymers that feature metal-ligand (ML) complexes are, in addition to the general nature of the monomer, significantly affected by the choice of ligand and metal salt. Indeed, the variation of these components can be used to alter the structural, thermal, mechanical, and viscoelastic properties over a wide ranges. Moreover, the dynamic nature of certain ML complexes can render the resulting metallosupramolecular polymers (MSPs) stimuli-responsive, enabling functions such as healing, reversible adhesion, and mechanotransduction. We here report MSPs based on the bidentate ligand 6-(1'-methylbenzimidazolyl) pyridine (MBP), which is easily accessible and forms threefold coordination complexes with various transition metal ions. Thus, a poly(ethylene-co-butylene) telechelic was end-functionalized with two MBP ligands and the resulting macromonomer was assembled with the triflate salts of either Zn2+, Fe2+, or Ni2+. All three MSPs microphase separate and adopt, depending on the metal ion and thermal history, lamellar or hexagonal morphologies with crystalline domains formed by the ML complexes. The melting transitions are well below 200 °C, and this permits facile (re)processing. Furthermore, defects can be readily and fully healed upon exposure to UV-light. While the three MSPs display similar moduli in the rubbery regime, their extensibility and tensile strength depend on the nature of the ML complex, which similarly affects the long-range order and dynamic behavior.
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The importance of protein glycosylation in the biomedical field requires methods that not only quantitate structures by their monosaccharide composition, but also resolve and identify the many isomers expressed by mammalian cells. The art of unambiguous identification of isomeric structures in complex mixtures, however, did not yet catch up with the fast pace of advance of high-throughput glycomics. Here, we present a strategy for deducing structures with the help of a deci-minute accurate retention time library for porous graphitic carbon chromatography with mass spectrometric detection. We implemented the concept for the fundamental N-glycan type consisting of five hexoses, four N-acetylhexosamines and one fucose residue. Nearly all of the 40 biosynthetized isomers occupied unique elution positions. This result demonstrates the unique isomer selectivity of porous graphitic carbon. With the help of a rather tightly spaced grid of isotope-labeled internal N-glycan, standard retention times were transposed to a standard chromatogram. Application of this approach to animal and human brain N-glycans immediately identified the majority of structures as being of the bisected type. Most notably, it exposed hybrid-type glycans with galactosylated and even Lewis X containing bisected N-acetylglucosamine, which have not yet been discovered in a natural source. Thus, the time grid approach implemented herein facilitated discovery of the still missing pieces of the N-glycome in our most noble organ and suggests itselfâin conjunction with collision induced dissociationâas a starting point for the overdue development of isomer-specific deep structural glycomics.
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Glicómica , Polisacáridos , Animales , Encéfalo , Fucosa , Glicosilación , HumanosRESUMEN
BACKGROUND: Epistemological biases in environmental epidemiology prevent the full understanding of how racism's societal impacts directly influence health outcomes. With the ability to focus on "place" and the totality of environmental exposures, environmental epidemiologists have an important opportunity to advance the field by proactively investigating the structural racist forces that drive disparities in health. OBJECTIVE: This commentary illustrates how environmental epidemiology has ignored racism for too long. Some examples from environmental health and male infertility are used to illustrate how failing to address racism neglects the health of entire populations. DISCUSSION: While research on environmental justice has attended to the structural sources of environmental racism, this work has not been fully integrated into the mainstream of environmental epidemiology. Epidemiology's dominant paradigm that reduces race to a mere data point avoids the social dimensions of health and thus fails to improve population health for all. Failing to include populations who are Black, Indigenous, and people of color (BIPOC) in health research means researchers actually know very little about the effect of environmental contaminants on a range of population health outcomes. This commentary offers different practical solutions, such as naming racism in research, including BIPOC in leadership positions, mandating requirements for discussing "race", conducting far more holistic analyses, increasing community participation in research, and improving racism training, to address the myriad of ways in which structural racism permeates environmental epidemiology questions, methods, results and impacts.
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Racismo , Racismo Sistemático , Salud Ambiental , Humanos , MasculinoRESUMEN
BACKGROUND: Diffuse gliomas are the most common malignant tumors of the central nervous system with poor treatment efficacy. Infiltration of immune cells into tumors during immunosurveillance is observed in multiple tumor entities and often associated with a favorable outcome. The aim of this study was to evaluate the infiltration of immune cells in gliomas and their association with cerebrospinal fluid (CSF) cytokine concentrations. METHODS: We applied immunohistochemistry in tumor tissue sections of 18 high-grade glioma (HGG) patients (4 anaplastic astrocytoma, IDH-wildtype WHO-III; 14 glioblastomas (GBM), IDH-wildtype WHO-IV) in order to assess and quantify leucocytes (CD45) and macrophages (CD68, CD163) within the tumor core, infiltration zone and perivascular spaces. In addition, we quantified the concentrations of 30 cytokines in the same patients' CSF and in 14 non-inflammatory controls. RESULTS: We observed a significantly higher percentage of CD68+ macrophages (21-27%) in all examined tumor areas when compared to CD45+ leucocytes (ca. 3-7%); CD163+ cell infiltration was between 5 and 15%. Compared to the tumor core, significantly more macrophages and leucocytes were detectable within the perivascular area. The brain parenchyma showing a lower tumor cell density seems to be less infiltrated by macrophages. Interleukin (IL)-7 was significantly downregulated in CSF of GBM patients compared to controls. Additionally, CD68+ macrophage infiltrates showed significant correlations with the expression of eotaxin, interferon-γ, IL-1ß, IL-2, IL-10, IL-13, IL-16 and vascular endothelial growth factor. CONCLUSIONS: Our findings suggest that the infiltration of lymphocytes is generally low in HGG, and does not correlate with cytokine concentrations in the CSF. In contrast, macrophage infiltrates in HGG are associated with CSF cytokine changes that possibly shape the tumor microenvironment. Although results point towards an escape from immunosurveillance or even exploitation of immune cells by HGG, further studies are necessary to decipher the exact role of the immune system in these tumors.
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Astrocitoma/líquido cefalorraquídeo , Neoplasias Encefálicas/líquido cefalorraquídeo , Citocinas/líquido cefalorraquídeo , Glioblastoma/líquido cefalorraquídeo , Leucocitos , Macrófagos , Adulto , Anciano , Anciano de 80 o más Años , Antígenos CD , Antígenos de Diferenciación Mielomonocítica , Astrocitoma/patología , Neoplasias Encefálicas/patología , Recuento de Células , Quimiocina CCL11/líquido cefalorraquídeo , Femenino , Glioblastoma/patología , Humanos , Inmunohistoquímica , Interferón gamma/líquido cefalorraquídeo , Interleucinas/líquido cefalorraquídeo , Leucocitos/citología , Linfocitos Infiltrantes de Tumor/citología , Macrófagos/citología , Masculino , Persona de Mediana Edad , Receptores de Superficie Celular , Microambiente Tumoral , Factor A de Crecimiento Endotelial Vascular/líquido cefalorraquídeoRESUMEN
AIMS: Data regarding the efficacy of catheter ablation in heart failure patients with severely dilated left atrium and reduced left ventricular ejection fraction (LVEF) are scanty. We sought to assess the efficacy of catheter ablation in patients with reduced LVEF and severe left atrial (LA) enlargement, and to compare it to those patients with preserved left ventricular function and equally dilated left atrium. METHODS AND RESULTS: Three patient groups with paroxysmal or persistent atrial fibrillation (AF) undergoing a first pulmonary vein isolation (PVI) were considered: Group 1 included patients with normal or mildly abnormal LA volume (≤41 mL/m2) and normal LVEF; Group 2 included patients with severe LA enlargement (>48 mL/m2) and normal LVEF; and Group 3 included patients with severe LA enlargement and reduced LVEF. Time to event analysis was used to investigate AF recurrences. The study cohort includes 439 patients; Group 3 had a higher prevalence of cardiovascular risk factors. LA enlargement was associated with a two-fold in risk of AF recurrence, on the contrary only a smaller non-significant increase of 30% was shown with the further addition of LVEF reduction. CONCLUSIONS: The long-term outcome of patients with severe LA dilatation and reduced LVEF is comparable to those with severe LA enlargement but preserved LVEF. Long-term efficacy of PVI is certainly affected by the enlargement of the left atrium, but less so by the addition of a reduced LVEF. CA remains the best strategy for rhythm control both in paroxysmal and persistent AF in this subgroup of patients.
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Fibrilación Atrial , Ablación por Catéter , Venas Pulmonares , Fibrilación Atrial/complicaciones , Fibrilación Atrial/diagnóstico , Fibrilación Atrial/cirugía , Ablación por Catéter/efectos adversos , Ablación por Catéter/métodos , Atrios Cardíacos/diagnóstico por imagen , Atrios Cardíacos/cirugía , Humanos , Venas Pulmonares/cirugía , Recurrencia , Volumen Sistólico , Resultado del Tratamiento , Función Ventricular IzquierdaRESUMEN
Self-healing or healable polymers can recuperate their function after physical damage. This process involves diffusion of macromolecules across severed interfaces until the structure of the interphase matches that of the pristine material. However, monitoring this nanoscale process and relating it to the mechanical recovery remain elusive. We report that studying diffusion across healed interfaces and a correlation of contact time, diffusion depth, and mechanical properties is possible when two metallosupramolecular polymers assembled with different lanthanoid salts are mended. The materials used display similar properties, while the metal ions can be tracked with high spatial resolution by energy-dispersive x-ray spectrum imaging. We find that healing actual defects requires an interphase thickness in excess of 100 nm, 10 times more than previously established for self-adhesion of smooth films of glassy polymers.
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Background Short ECG P-wave duration has recently been demonstrated to be associated with higher risk of atrial fibrillation (AF). The aim of this study was to assess the rate of AF recurrence after pulmonary vein isolation in patients with a short P wave, and to mechanistically elucidate the observation by computer modeling. Methods and Results A total of 282 consecutive patients undergoing a first single-pulmonary vein isolation procedure for paroxysmal or persistent AF were included. Computational models studied the effect of adenosine and sodium conductance on action potential duration and P-wave duration (PWD). About 16% of the patients had a PWD of 110 ms or shorter (median PWD 126 ms, interquartile range, 115 ms-138 ms; range, 71 ms-180 ms). At Cox regression, PWD was significantly associated with AF recurrence (P=0.012). Patients with a PWD <110 ms (hazard ratio [HR], 2.20; 95% CI, 1.24-3.88; P=0.007) and patients with a PWD ≥140 (HR, 1.87, 95% CI, 1.06-3.30; P=0.031) had a nearly 2-fold increase in risk with respect to the other group. In the computational model, adenosine yielded a significant reduction of action potential duration 90 (52%) and PWD (7%). An increased sodium conductance (up to 200%) was robustly accompanied by an increase in conduction velocity (26%), a reduction in action potential duration 90 (28%), and PWD (22%). Conclusions One out of 5 patients referred for pulmonary vein isolation has a short PWD which was associated with a higher rate of AF after the index procedure. Computer simulations suggest that shortening of atrial action potential duration leading to a faster atrial conduction may be the cause of this clinical observation.
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Potenciales de Acción , Fibrilación Atrial , Ablación por Catéter , Electrocardiografía/métodos , Sistema de Conducción Cardíaco , Complicaciones Posoperatorias , Potenciales de Acción/efectos de los fármacos , Potenciales de Acción/fisiología , Adenosina/farmacología , Antiarrítmicos/farmacología , Fibrilación Atrial/diagnóstico , Fibrilación Atrial/fisiopatología , Ablación por Catéter/efectos adversos , Ablación por Catéter/métodos , Simulación por Computador , Femenino , Sistema de Conducción Cardíaco/efectos de los fármacos , Sistema de Conducción Cardíaco/fisiopatología , Humanos , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias/diagnóstico , Complicaciones Posoperatorias/etiología , Complicaciones Posoperatorias/fisiopatología , Venas Pulmonares/cirugía , Recurrencia , Canales de Sodio/fisiologíaRESUMEN
Research is needed to understand the presence of the SARS-CoV-2 virus in semen, sexual transmissibility, and impact on sperm quality. Several studies have examined men recovering from COVID-19, but large-scale community-based testing is needed to ascertain the effects on the male reproductive tract, and the potential for prolonged transmission.
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COVID-19/virología , SARS-CoV-2/patogenicidad , Semen/virología , Enfermedades Virales de Transmisión Sexual/virología , Espermatozoides/virología , Animales , COVID-19/diagnóstico , COVID-19/transmisión , Prueba de COVID-19 , Interacciones Huésped-Patógeno , Humanos , Masculino , Análisis de Semen , Enfermedades Virales de Transmisión Sexual/diagnóstico , Enfermedades Virales de Transmisión Sexual/transmisión , Espermatozoides/patología , VirulenciaRESUMEN
A number of recent studies have shown the importance of the mammalian gut microbiome in host health. In the context of endangered species, a few studies have examined the relationship between the gut microbiome in wild versus captive populations due to digestive and other health issues. Unfortunately, the results seem to vary across taxa in terms of captive animals having higher, lower, or equivalent microbiome diversity relative to their wild counterparts. Here, we focus on the black rhinoceros as captive animals suffer from a number of potentially dietary related health effects. We compared gut microbiomes of wild and captive black rhinos to test for differences in taxonomic diversity (alpha and beta) and in functional diversity of the microbiome. We incorporated a more powerful metagenomic shotgun sequencing approach rather than a targeted amplification of the 16S gene for taxonomic assignment of the microbiome. Our results showed no significant differences in the alpha diversity levels between wild and captive black rhinos, but significant differences in beta diversity. We found that bacterial taxa traditionally associated with ruminant guts of domesticated animals had higher relative abundances in captive rhinos. Our metagenomic sequencing results suggest that unknown gut microbes of wild rhinos are being replaced by those found in conventional human-domesticated livestock. Wild rhinos have significantly different functional bacterial communities compared to their captive counterparts. Functional profiling results showed greater abundance of glycolysis and amino acid synthesis pathways in captive rhino microbiomes, representing an animal receiving sub-optimal nutrition with a readily available source of glucose but possibly an imbalance of necessary macro and micronutrients. Given the differences observed between wild and captive rhino gut microbiomes, we make a number of recommendations for potentially modifying captive gut microbiome to better reflect their wild counterparts and thereby hopefully improve overall rhino health in captivity.
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Microbioma Gastrointestinal/genética , Microbiota/genética , Perisodáctilos/microbiología , Animales , Animales Salvajes/microbiología , Animales de Zoológico/microbiología , Heces/microbiología , Mamíferos/microbiologíaRESUMEN
Mucosal plasma cells (PC) and Ig production are essential to fend pathogens and to maintain mucosal homeostasis. In human Helicobacter pylori infection, mucosal PC express inducible NO synthase (iNOS), which positively correlates with clearance of experimental human infection. To characterize Ig genes and specificities of antral mucosal iNOS+ and iNOS- PC in H. pylori infection, we sequenced rearranged Ig genes from single cell-sorted PC from biopsy specimens of chronically infected patients and analyzed them with respect to their molecular features. The binding specificity of individual PC's Ig was determined following recombinant expression. We identified high rates of somatic hypermutations, especially targeting RGYW/WRCY hotspot motifs in the individual Ig genes, indicating T cell-dependent maturation. For seven of 14 recombinantly expressed Ig, Ag specificity could be determined. Two clones reacted to H. pylori proteins, and five were found to be polyreactive against LPSs, dsDNA, and ssDNA. All specific Ig originated from iNOS+ PC. H. pylori-specific Ig are encoded by V and J family genes previously shown to be also used in rearranged Ig loci of MALT B cell lymphomas. In summary, mucosal iNOS+ PC producing H. pylori-specific Ig accumulate in infection and appear to be a product of T cell-dependent B cell maturation. Moreover, the Ig's molecular features partly resembled that of MALT B cell lymphoma Ig genes, suggestive of a mechanism in which a progressive molecular evolution of pathogen-specific B cells to MALT B cell lymphoma occurs.
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Infecciones por Helicobacter/inmunología , Helicobacter pylori/fisiología , Mucosa Intestinal/inmunología , Linfoma de Células B de la Zona Marginal/inmunología , Células Plasmáticas/inmunología , Antro Pilórico/inmunología , Linfocitos T/inmunología , Adulto , Proteínas Bacterianas/inmunología , Enfermedad Crónica , Epítopos , Femenino , Humanos , Inmunoglobulinas/metabolismo , Lipopolisacáridos/inmunología , Linfoma de Células B de la Zona Marginal/genética , Masculino , Persona de Mediana Edad , Óxido Nítrico Sintasa de Tipo II/metabolismo , Hipermutación Somática de Inmunoglobulina , Adulto JovenRESUMEN
Equipping a polymeric material with the ability to heal an inflicted damage is a crucial advantage for many applications. The incorporation of reversible and dynamic supramolecular interactions into polymeric systems has proven to be a promising route towards such materials. In this article, recent developments in the field of healable materials are highlighted with a particular focus on the design principles, driving forces, and mechanisms that allow healing to occur.
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While coupling mechanical and chemical processes is widespread in living organisms, the idea to harness the mechanically induced dissociation of weak covalent and non-covalent bonds to create artificial materials that respond to mechanical stimulation has only recently gained attention. Here we summarize our activities that mainly revolve around the exploitation of non-covalent interactions in (supramolecular) polymeric materials with the goal to translate mechanical stresses into useful, pre-defined events. Focusing on mechano- chromic polymers that alter their optical absorption or fluorescence properties, several new operating principles, mechanosensitive entities, and materials systems were developed. Such materials are expected to be useful for technical applications that range from the detection of very small forces in biological systems to the monitoring of degradation processes and damage in coatings and structural objects.
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BACKGROUND & AIMS: The adductor pollicis muscle thickness (APMT) is a promising method for evaluation of muscle loss and, consequently, malnutrition in adult and elderly patients. However, to date, there have been no studies of its applicability to the pediatric population. Within this context, we sought to evaluate the association of APMT with anthropometric variables, body mass index (BMI), pediatric Subjective Global Assessment (SGA) of nutrition, nutritional screening, and clinical outcomes in hospitalized pediatric patients. METHODS: This was a cross-sectional study of inpatients aged 4-8.9 years, recruited via convenience sampling from a pediatric hospital in Porto Alegre, Rio Grande do Sul, Brazil. Data collection took place between December 2014 and February 2016. Patients admitted to the intensive care unit, those unable to feed orally, and those with cerebral palsy or Down syndrome were excluded from the study. General and socioeconomic information was collected and the SGA Ped and STRONGkids were administered at hospital admission. Clinical data were collected from the electronic medical record. Anthropometric parameters and APMT were measured by properly calibrated examiners. Data analysis was carried out in SPSS version 21.0. The significance level was set at 5%. RESULTS: The sample consisted of 447 patients. Most (55.9%) were male; the mean age was 6.2 ± 1.4 years. Low APMT was significantly associated with underweight, short stature, low body fat percentage, and poor muscle reserve (p < 0.001). There were also significant associations of moderate and severe malnutrition (assessed by the SGA Ped) and high nutritional risk (assessed by the STRONGkids instrument) with reduced APMT (p < 0.001). Regarding clinical outcomes, a longer hospital stay was observed in patients with reduced APMT (p = 0.001). A receiver operating characteristic (ROC) curve, plotted considering the SGA Ped as the gold standard, suggested APMT cutoff points of 10.2 mm for boys and 9.5 mm for girls. Stratification by age yielded APMT cutoff points of 9.8 mm for boys younger than 6 years and 10.2 mm for those older than 6 years, and 9.2 mm and 9.8 mm for girls younger and older than 6 years, respectively. CONCLUSION: The APMT is an efficient parameter for the detection of malnutrition in hospitalized pediatric patients.
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Hospitalización , Músculo Esquelético/fisiología , Evaluación Nutricional , Estado Nutricional/fisiología , Pulgar/fisiología , Niño , Trastornos de la Nutrición del Niño/diagnóstico , Preescolar , Estudios Transversales , Femenino , Humanos , Masculino , Curva ROCRESUMEN
The cell surface of the oral pathogen Tannerella forsythia is heavily glycosylated with a unique, complex decasaccharide that is O-glycosidically linked to the bacterium's abundant surface (S-) layer, as well as other proteins. The S-layer glycoproteins are virulence factors of T. forsythia and there is evidence that protein O-glycosylation underpins the bacterium's pathogenicity. To elucidate the protein O-glycosylation pathway, genes suspected of encoding pathway components were first identified in the genome sequence of the ATCC 43037 type strain, revealing a 27-kb gene cluster that was shown to be polycistronic. Using a gene deletion approach targeted at predicted glycosyltransferases (Gtfs) and methyltransferases encoded in this gene cluster, in combination with mass spectrometry of the protein-released O-glycans, we show that the gene cluster encodes the species-specific part of the T. forsythia ATCC 43037 decasaccharide and that this is assembled step-wise on a pentasaccharide core. The core was previously proposed to be conserved within the Bacteroidetes phylum, to which T. forsythia is affiliated, and its biosynthesis is encoded elsewhere on the bacterial genome. Next, to assess the prevalence of protein O-glycosylation among Tannerella sp., the publicly available genome sequences of six T. forsythia strains were compared, revealing gene clusters of similar size and organization as found in the ATCC 43037 type strain. The corresponding region in the genome of a periodontal health-associated Tannerella isolate showed a different gene composition lacking most of the genes commonly found in the pathogenic strains. Finally, we investigated whether differential cell surface glycosylation impacts T. forsythia's overall immunogenicity. Release of proinflammatory cytokines by dendritic cells (DCs) upon stimulation with defined Gtf-deficient mutants of the type strain was measured and their T cell-priming potential post-stimulation was explored. This revealed that the O-glycan is pivotal to modulating DC effector functions, with the T. forsythia-specific glycan portion suppressing and the pentasaccharide core activating a Th17 response. We conclude that complex protein O-glycosylation is a hallmark of pathogenic T. forsythia strains and propose it as a valuable target for the design of novel antimicrobials against periodontitis.
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The utility of therapeutic vaccination of bulls against Tritrichomonas foetus has been advocated in previous studies, but anecdotal reports suggest this practice does not clear infections and may additionally confound diagnostic testing by reducing parasite burdens below detectable limits. The objective of this study was to characterize the systemic humoral immune response to therapeutic vaccination in T. foetus-infected bulls over a period of four months using an indirect ELISA and to compare the dynamics of this response to culture and PCR results to establish the existence of a relationship (or lack thereof) between immunization and infection status. A study population of 4- to 6-year-old T. foetus-infected beef bulls (nâ¯=â¯20) was divided equally into a treatment group and a control group. The treatment group received two doses of commercially prepared whole cell killed vaccine 2 weeks apart while the control group received injections of vaccine diluent. Blood samples were collected at each injection and at 4 subsequent dates every 4 weeks thereafter (i.e. 0, 2, 6, 10, 14, and 18 wks) to measure IgG1 and IgG2 antibody subisotype response via an indirect ELISA. Preputial smegma samples were collected at the four monthly intervals following vaccination for diagnosis of infection via InPouch™ culture, Modified Diamond's Medium (MDM) culture, and PCR. Humoral response for both IgG isotypes from week 2 through week 18 were significantly increased in vaccinates compared to controls. No significant decrease in infection prevalence was detected in the treatment group for any of the diagnostic methods used. The apparent lack of pathogen clearance during a stimulated immune response suggests that therapeutic vaccination may not be a useful T. foetus management practice.
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Inmunidad Humoral , Infecciones Protozoarias en Animales/prevención & control , Vacunas Antiprotozoos/inmunología , Tritrichomonas foetus/inmunología , Vacunación/veterinaria , Animales , Anticuerpos Antiprotozoarios/sangre , Bovinos , Inmunoglobulina G/sangre , Masculino , Infecciones Protozoarias en Animales/inmunologíaRESUMEN
The mucosal immune system is relevant for homeostasis, immunity, and also pathological conditions in the gastrointestinal tract. Inducible NO synthase (iNOS)-dependent production of NO is one of the factors linked to both antimicrobial immunity and pathological conditions. Upregulation of iNOS has been observed in human Helicobacter pylori infection, but the cellular sources of iNOS are ill defined. Key differences in regulation of iNOS expression impair the translation from mouse models to human medicine. To characterize mucosal iNOS-producing leukocytes, biopsy specimens from H. pylori-infected patients, controls, and participants of a vaccination trial were analyzed by immunohistochemistry, along with flow cytometric analyses of lymphocytes for iNOS expression and activity. We newly identified mucosal IgA-producing plasma cells (PCs) as one major iNOS(+) cell population in H. pylori-infected patients and confirmed intracellular NO production. Because we did not detect iNOS(+) PCs in three distinct infectious diseases, this is not a general feature of mucosal PCs under conditions of infection. Furthermore, numbers of mucosal iNOS(+) PCs were elevated in individuals who had cleared experimental H. pylori infection compared with those who had not. Thus, IgA(+) PCs expressing iNOS are described for the first time, to our knowledge, in humans. iNOS(+) PCs are induced in the course of human H. pylori infection, and their abundance seems to correlate with the clinical course of the infection.
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Helicobacter pylori/inmunología , Inmunoglobulina A/inmunología , Óxido Nítrico Sintasa de Tipo II/biosíntesis , Células Plasmáticas/enzimología , Células Plasmáticas/inmunología , Biopsia , Femenino , Mucosa Gástrica/microbiología , Infecciones por Helicobacter/inmunología , Infecciones por Helicobacter/microbiología , Humanos , Inmunoglobulina A/biosíntesis , Inmunohistoquímica , Masculino , Óxido Nítrico/metabolismo , Estudios Prospectivos , Antro Pilórico/microbiología , Antro Pilórico/patologíaRESUMEN
Objetivos: Comparar a Avaliação Nutricional Subjetiva Global (ANSG) e a Triagem de Risco para Estado Nutricional e Crescimento (STRONGkids) com a avaliação antropométrica, na admissão hospitalar, e associá-las ao tempo de internação em crianças hospitalizadas. Métodos: Estudo transversal com pacientes de 4 a 8,9 anos internados em um hospital pediátrico de Porto Alegre, Rio Grande do Sul. A amostragem foi realizada por conveniência, e a coleta de dados ocorreu entre junho e outubro de 2014. Não foram incluídos pacientes internados em Unidade de Terapia Intensiva e aqueles sem condições de alimentação por via oral. Foram coletadas informações gerais e socioeconômicas, e aplicados os questionários de ANSG e STRONGkids na admissão hospitalar. Dados clínicos e antropométricos foram coletados do prontuário eletrônico. Resultados: Avaliaram-se 317 pacientes com idade média de 76,1±17,5 meses e, na maioria, com motivo de internação cirúrgico (21,5%). Segundo a avaliação antropométrica, 5% dos pacientes eram desnutridos, 74,1% eram eutróficos e 20,8% apresentavam excesso de peso. Houve associação significativa entre a desnutrição classificada pela avaliação antropométrica e a desnutrição moderada e grave definida pela ANSG (p<0,001). Também houve associação significativa entre excesso de peso, eutrofia e desnutrição, classificados pela avaliação antropométrica, e risco nutricional baixo, médio e alto, respectivamente (p<0,001). Houve concordância significativa, porém muito fraca, entre a STRONGkids e a avaliação antropométrica (kappa=0,148; p=0,001). Conclusões: Todas as avaliações feitas por meio das ferramentas testadas associaram-se (embora em fraca intensidade) com o tempo de internação hospitalar. Além disso, a STRONGkids apresentou maior concordância, embora ainda fraca, com a avaliação antropométrica quando comparada à ANSG. São necessários mais estudos verificando a concordância dessas ferramentas com outros métodos objetivos de avaliação nutricional.
Aims: To compare the Subjective Global Nutritional Assessment (SGNA) and the Screening Tool for Risk on Nutritional Status and Growth (STRONGkids) protocols with anthropometric measurements at admission, and associate them to length of stay in hospitalized children. Methods: Cross-sectional study with patients from four to 8.9 years admitted to a pediatric hospital in Porto Alegre, Rio Grande do Sul, Brazil. The sample was selected by convenience, and data collection occurred between June and October 2014. Patients in the Intensive Care Unit and those unable to feed orally were not included. We collected general and socioeconomic information and applied STRONGkids and SGNA protocols at admission. Clinical and anthropometric data were retrieved from electronic medical records. Results: We evaluated 317 patients with a mean age of 76.1±17.5 months, most of them admitted for surgery (21.5%). According to anthropometric measurements, 5% of patients were malnourished, 74.1% had normal weight and 20.8% were overweight. There was a statistically significant association between malnutrition classified by anthropometric measurements and moderate and severe malnutrition defined by SGNA (p<0.001). There was also a significant association between overweight, normal weight and malnutrition classified by anthropometric measurements and low, medium and high nutritional risk, respectively (p<0.001). There was a statistically significant agreement, although very weak, between STRONGkids and anthropometric measurements (kappa=0.148; p=0.001). Conclusions: All protocols were associated (though in low intensity) to length of hospital stay. In addition, STRONGkids showed greater agreement, although still weak, with anthropometric measurements when compared to the SGNA. Further studies are needed to verify the agreement of these protocols with other objective methods of nutritional assessment.