The first outbreak of Lumpy Skin Disease in Indonesia.

This study describes the first outbreak of Lumpy Skin Disease (LSD) in cattle in the Bengkalis region, Indonesia, and vaccination to control the epidemic. Data on the outbreak and vaccination was obtained from the local veterinary authority of the Bengkalis region, Indonesia. Climatological data was provided by the Meteorological, Climatological, and Geophysical Agency of Riau Province. Over the 5.5 months, the outbreak caused 10.4% (94/906) morbidity and 0.6% (6/906) mortality of cattle on infected farms. Temporally, three epidemic waves occurred during the outbreak period. Villages with cattle populations of > 150 animals (n = 36) were 5.3 times more likely to be infected with LSD compared to villages with smaller cattle populations (n = 107) (CI: 2.56-10.90, P < 0.01). The vaccination campaign covered 43.8% of cattle in villages within a 10 km radius of the cases. However, vaccination in villages with larger cattle populations (n = 29) was 0.63 less likely to cover 50% of the cattle populations compared to villages with smaller cattle populations (n = 41) (CI: 0.39-1.02, P = 0.05). By the time the first two and the major waves ceased, vaccination had covered only 0.0% (n = 6036), 27.8% (n = 6,036) and 9.7% (n = 5,697) of the cattle in the 10 km radius of the respective spatial clusters. The outbreak was statistically associated with rainfall and its interaction with temperature (F(2, 13) = 5.822, R2 = 0.47, P = 0.016). This study indicates that the LSD outbreak had low morbidity and mortality. Despite the low vaccination rate, the outbreak ceased, possibly due to plummeting of the abundance of insect vectors.

The Epidemiology and Control of Bovine Viral Diarrhoea Virus in Tropical Indonesian Cattle.

This review aims to update the knowledge of the epidemiology of Bovine viral diarrhoea virus (BVDV) in Indonesia and Southeast Asia and provide a perspective on the control options for BVDV in the Indonesian cattle population in the future. Studies on BVDV in Indonesia, since its first report in that country, and the updated beef and dairy cattle industries are reviewed. In ten of 34 provinces, BVDV is endemic. The subgenotypes of BVDV-1a and BVDV-1c are predominant in Indonesian cattle. However, BVDV is currently not a priority disease to control in Indonesia. Cattle imports from Australia appear to be potentially the most significant source of transmission of BVDV into native cattle, but the control of BVDV conducted in the local quarantine facilities is currently not achieving the aim of controlling BVDV; thus, complementary measures are needed. With the small-scale nature of the vast majority of cattle breeding in the country, the control of BVDV in provinces in which cattle breeding is economically essential may need to be organised by regional and provincial governments. Gaps in our knowledge of BVDV are identified in this review, and strategies for the control of BVDV in Indonesia are discussed.

Factors affecting enduring participation in a pig farming program in Southern Papua, Indonesia.

In mid-2016, the local government of Papua, Indonesia, launched a subsidised program to improve pig farming in the area, with 250 participants. This study aimed to investigate factors associated with enduring participation in pig farming among the program participants. Two years after the commencement of the program, one hundred of the 250 participants were recruited into the study and divided into two groups: participants who continued to engage in pig farming ("remain") and those who had quit pig farming ("quit"). Data were collected from interviews, including personal data and events on the farms between April 2017 and March 2018. Multiple correspondence analysis, followed by a two-step cluster analysis and multivariate regression, was used to explore factors associated with the durability of pig farmings. Results indicated that associated factors included feed and water security, the use of concrete material for the pig house floor, the avoidance of swill feeding, reduced pig mortality, and continued pig husbandry training. This study highlights that a good feeding regimen and effective control of pig diseases should be priority pig husbandry techniques to be extended through training and assistance to improve traditional pig farming in Papua.

Development of ELISA against milk haptoglobin for diagnosis of subclinical mastitis in goats.

The study described the development of a haptoglobin-based diagnostic tool for mastitis in Ettawa crossbreed goats. Fifty eight milk samples were collected from a flock of goats in Yogyakarta, Central Java, Indonesia. All samples were tested for mastitis using the California Mastitis Test (CMT), Somatic Cell Count (SCC), and Polymerase Chain Reaction (PCR) to identify Staphylococcus aureus, Streptococcus uberis and Streptococcus agalactiae. The presence of haptoglobin mRNA and proteins in the milk somatic cells was detected using Sanger sequencing and SDS-PAGE, respectively. Milk haptoglobin levels were subsequently estimated using an indirect enzyme-linked immunosorbent assay (ELISA) developed in this study. The Receiver Operating Characteristic (ROC) analysis was performed to compare the sensitivity and specificity of CMT, SCC, and the ELISA using the PCR as the reference standard. Kappa test was used to determine the agreement between the three imperfect tests. Results indicated that somatic cells of goat milk expressed a haptoglobin mRNA with a size of 174 bp and two haptoglobin proteins with molecular weights of 18 kDa and 32 kDa. The PCR test showed that 81% of samples were diagnosed positive for mastitis. At a specificity level of 50%, the ROC indicated that the ELISA was more sensitive compared to SCC or CMT (consecutively, 96%, 94%, and 92%). Kappa values between haptoglobin ELISA and CMT or SCC were high (0.84 and 0.81, respectively). This study indicates that somatic cells of goat milk were capable of synthesizing and secreting haptoglobin. Milk haptoglobin can be a potential target for an early detection of mastitis in goats.

Productivity, absence of a bull and endoparasitic nematodiosis in beef cattle farms in an upland area of East Java, Indonesia.

BACKGROUND AND AIM: Cattle are an important economic asset for the rural community in East Java Province, Indonesia. The study aimed to provide updated data of cattle farm demography, productivity, as well as the role of the absence of a bull and nematodiosis in reduced productivity of beef cattle in an upland rural area of the province. MATERIALS AND METHODS: The study was conducted in Sukowono village, Bondowoso region. A Census survey was conducted to collect data through interviews with farmers. Further, 102 fecal samples were taken systematically and processed using a double centrifugation method to investigate the endoparasitic nematodiosis in the cattle population. The demographic data, productivity, and nematodiosis were analyzed descriptively. The difference between proportions was analyzed using Chi-square with 95% confidence limit. The associations were described in risk ratio with 95% confidence interval (CI). RESULTS: The total cattle population was 814 heads; the range of farm size was 1-7 (median: 2) cattle. Female cattle comprised 81.8% (666/814) of the cattle population but, only 5.5% (23/422) farmers kept both bull and mature female cattle. Pregnancy rate was 26.8% (145/542) of mature female cattle. The delayed first calving time appeared in 24.8% (62/250) of heifers and calving interval of >14 months occurred in 83.2% (149/179) of multiparous cows. The prevalence of endoparasitic nematodiosis was 43.1% (44/102, 95%, CI: 38.1-52.1%). Either the absence of the bull or the nematodiosis did not associate with pregnancy rate or calving interval of cows. CONCLUSION: This study indicates that the productivity of the cattle in the study area was low but may not associate with the absence of a bull or nematodiosis.

Infection with Bovine Viral Diarrhea Virus in Cattle in Southern Papua, Indonesia.

The aim of this preliminary study was to determine the prevalence of Bovine Viral Diarrhea Virus (BVDV) and its association with reproductive problems in cattle in Timika, Southern Papua, Indonesia, an emerging area for beef production. Serum from 77 beef cattle was collected from four villages and tested, using both antibody and antigen ELISA kits for BVDV. Data of the villages of origin, age, breed, sex and the number of parities of the cattle were collected. The pregnancy status of the cattle was determined by rectal examination. Results showed that the prevalence of BVDV antibody in individual cattle in Timika was 11.7% (CI: 6.1 - 20.0%), while at the farm level the antibody prevalence was 18.4% (CI: 8.9 - 33.7%).). Seropositivity for BVDV increased with age (P=0.02), from 0% (CI: 0 - 37.2%) in less than two-years old to 28.6% (CI: 11.3 - 55.0%) in cattle older than eight years of age. BVDV antibody was 2.9 times more prevalent in non-pregnant cows than in antibody negative cows (CI: 1.02 - 8.14, P=0.04). BVD antigen was not detected in the present study. This study reported for the first time, evidence of infection with BVDV in cattle in Papua and indicated that BVDV infection may be associated with infertility.

Investigations of selected pathogens among village pigs in Central Papua, Indonesia.

Village pig husbandry is an important part of livestock production in Papua Province, Eastern Indonesia. However, high level of disease and mortality constrains production. The aim of this study was to investigate the prevalence of the selected pathogens in village pigs in the Jayawijaya Region of Papua Province, Indonesia. Two studies were conducted: Study 1 determined the prevalence of selected pathogens in dead or moribund pigs sent to the main local market for sale. Study 2 recorded the prevalence of the selected pathogens, on pig farms in the Subdistrict of Wamena that had not recorded a case of pig mortality during the duration of Study 1. Blood samples of individuals from both groups were tested for CSF antigen and antibody, as well as antibody against PCV2. Organs with evident pathological changes from Study 1 and tonsilar swabs from Study 2 were subjected to bacteriological culture and identification of Streptococcus suis and Streptococcus zooepidemicus. Faecal samples from both studies were examined for eggs of strongyle parasites, Trichuris suis, Ascaris suum, Strongyloides ransomi and coccidia. The main infections in both studies were CSF, PCV2 and strongyle parasites, but prevalence was higher in Study 1 (P < 0.05). T. suis and S. zooepidemicus were prevalent in pigs in Study 1, but rare in healthy pigs (P < 0.05). Infections with coccidia, A. suum and S. ransomi were common but did not differ between groups (P < 0.05), with S. suis infections uncommon in both studies. This suggests that infections with CSF, PCV2, strongyle and T. suis are important pathogens in village pig farms in Jayawijaya. Local pig husbandry practices, such as confining pigs and heat-treating pig feeds, may be practical solutions to help minimize infection in village pigs in Jayawijaya.

Traditional pig farming practices and productivity in the Jayawijaya region, Papua Province, Indonesia.

The objective of the current survey was to provide an update on pig farming practices in the Jayawijaya region, Papua Province, Indonesia. A structured semi-close-ended questionnaire was used to interview 367 farmers across the Jayawijaya region. Results showed that farms, on average, comprised of 8.8 pigs (CI 8.5-9.1). The average litter size was 6.0 (CI 5.7-6.3) piglets, the farrowing frequency was once a year, and the annual mortality rate was 50.2% (CI 48.4-51.9). On average, 43.4% farms (CI 36.4-50.7) allowed pigs to roam freely during daylight hours. Farmers used pigs for their own consumption (62.4%, CI 57.4-67.4), as a gift (56.6%, CI 51.5-61.7), or for sale (50.7%, CI 45.6-55.8). Veterinary services were used intensively by just 11.7% of farmers (CI 8.2-16.5). Furthermore, 34.2% (CI 29.3-39) of farmers would sell sick pigs, and 63.1% (CI 58.2-68.1) would slaughter and consume them. It was also recorded that 68.6% of farmers (CI 63.7-73.4) would eat sick pigs that had died naturally. These findings suggest that traditional pig farms in Jayawijaya are of low productivity. Moreover, the free roaming of pigs and the sale and consumption of sick pigs have the potential to allow pathogens to circulate between pig and human populations.