Clinicopathological and bacteriological studies on lamb bacterial enteritis and monitoring the oregano oil and vitamins A,D3,E effect on its treatment.

OBJECTIVE: The objective of the study was to assess the effect of A,D3,E (I/M) and oregano oil extract 15% on some clinicopathological parameters during lamb bacterial enteritis treatment. MATERIALS AND METHODS: Sixty Barki lambs, 20 apparently healthy (control group) and, 40 suffered from bacterial enteritis [enteric group (EG)], were subdivided into four treated groups (TGs): antibiotic group (AG), antibiotic + A,D3,E group (A + A,D3,E), antibiotic + oregano oil (AOG), and oregano group (OG). Fecal swabs were collected from EG then aseptically cultured, isolated, phenotypically identified, genotypically confirmed, and sequenced by PCR 16srRNA. Paper disk diffusion test was used for estimation of oregano oil extract 15% antibacterial activity. After blood sample aspiration from all animals, they were clinicopathologically and statistically analyzed. RESULTS: Escherichia coli, followed by Salmonella species and then Klebsiella species, was the main causative agents of lamb diarrhea and were susceptible to oregano oil extract 15%. A + A,D3,E and AOG showed significant (p < 0.05) enhancement of some clinicopathological parameters more than AG or OG. Matrix metalloproteinases (MMP-2 and MMP-9) and total antioxidant capacity (TAC), yielded area under the curve, sensitivity, negative predictive value as 1, 100% and 100% respectively, were determined in both EG and TGs. CONCLUSION: Oregano oil extract 15% has good antibacterial properties against enteric bacteria in vitro and in vivo. The combination between antibiotic and antioxidant vitamins or oregano plant extract of 15% has a good impact on some clinicopathological alterations in lamb bacterial enteritis treatment. TAC, MMP-9, and MMP-2 may be good markers for the disease and its treatment follow-up.

Ultrastructural alterations in sperm formation of the beetle, Blaps polycresta (Coleoptera: Tenebrionidae) as a biomonitor of heavy metal soil pollution.

Little is known about ultrastructural alterations induced by heavy metals pollution in insects. Therefore, the main objective of the present study is to elucidate ultrastructural changes in sperm formation of the tenebrionid beetle, Blaps polycresta as a biomonitor of heavy metal soil pollution. Metal percentages in testicular tissues of adult insects collected from reference and polluted sites were estimated using energy-dispersive X-ray microanalysis (EDX). Only cadmium, among eight detected metals, showed significantly higher percentages in the polluted testes compared with the reference ones. Ultrastructure investigation revealed severe alterations both in spermatogenic and spermiogenic stages of the polluted insects. Some cells were totally eroded. No spermatozoa were observed in all the examined cysts. Most degenerations were confined to the flagella of spermatids having enlarged vacuolated cytoplasm and malformed mitochondrial derivatives. Groups of multiple axial filaments were appeared in the form of bi-and tetra-flagellate spermatids. Electron dense vesicles were observed in almost all stages of the polluted testes. It is a novel trend in which ultrastructural alterations in sperm formation of insects could be used as a promising biomonitoring and risk assessment tool for heavy metal soil pollution.

Ultrastructure alterations induced by gamma irradiation in spermiogenesis of the ground beetle, Blaps sulcata: reference to environmental radiation protection.

Ultrastructure alterations in spermiogenesis of the ground beetle, Blaps sulcata (Coleoptera: Tenebrionidae) were studied in normal adult males and in two male groups irradiated by gamma rays of 3 and 16 µSv/h dose rate. Ultrastructure examination of irradiated sperms revealed many alterations both in the head and in the flagellum regions of mature sperm. Alterations of the head region included nuclei with uncondensed chromatin materials and irregular nuclear envelope. Abnormal flagella contained malformed mitochondrial derivatives and damaged axonemes in addition to the absence of accessory bodies. Bi- and multi-flagellate sperms (with two, three, and four axonemes) were the most common alterations. Sperm cells with residual bodies were also obtained. Agglutinated sperms and sperms with enlarged and/or vacuolated cytoplasm were common. Sperm abnormalities were more pronounced in males irradiated by 16 µSv/h dose rate than those irradiated by 3 µSv/h. Spermiogenesis alterations induced by irradiation of B. sulcata may be used as a monitoring model for predicting the effects of environmental radioactivity.

X-ray metal assessment and ovarian ultrastructure alterations of the beetle, Blaps polycresta (Coleoptera, Tenebrionidae), inhabiting polluted soil.

X-ray analysis was applied to estimate the percentages of heavy metals in ovarian tissues of the tenebrionid beetle, Blaps polycresta. Calcium, phosphorus, sulfur, cadmium, copper, and zinc were the most common detected metals in ovaries of insects collected from reference and polluted sites. Only cadmium showed significantly higher percentages in the polluted ovaries compared with the reference ones. Ultrastructure investigation revealed severe alterations in polluted ovaries both in the tropharium and in the vitellarium. Contraction of nuclear membrane of trophocytes was observed; therefore, cavities and spaces appeared in the cytoplasm followed by nuclear pyknosis. In the vitellarium, fragmentation of chromatin materials in nuclei of the follicular cells was detected. The cytoplasm was poor in the rough endoplasmic reticulum and mitochondria. Damage of yolk bodies occurred in addition to break off in the layer of microvilli. Accumulation of electron-dense vesicles and multivesicular bodies were observed in both reference and polluted ovaries. These alterations in ovarian ultrastructure of B. polycresta show the severe impact of cadmium pollution on cell organelles of insects and could be used as an interesting tool for monitoring heavy metals inside the body organs due to soil pollution.

Ecological, morphological, and histological studies on Blaps polycresta (Coleoptera: Tenebrionidae) as biomonitors of cadmium soil pollution.

Soil pollution in Egypt became far more serious than before due to either the heavy usage of different toxic pesticides or aerosol deposition of industrial pollutants. The present mentioned ground beetle, Blaps polycresta Tschinkel 1975 (Coleoptera: Tenebrionidae), showed ecological, morphological, and histological alterations in adult insects as biomonitors. Two cultivated sites (reference and polluted) were chosen for sampling the insects. The results indicated a significant increase in soil cadmium concentration of the polluted site leading to sex-specific difference in cadmium accumulation in gonads and alimentary canal of insects that being higher in males than females. The cadmium pollution leads significantly to a decrease in population density, a reduction in body weight, an increase in mortality rate, and an increase in sex ratio of the insects. The results also revealed a striking decrease in body length of the polluted insects with a marked increase in the percentage of deformed gonads and alimentary canal of both sexes. Some histopathological alterations were also recorded in testis, ovary, and midgut of the polluted insects. Our results confirmed that beetles are a good bioindicator for soil pollution, and the different studied parameters could be easily employed as sensitive monitors for cadmium soil pollution.