RESUMEN
Fibrillar collagens I and III, nonfibrillar collagen IV, and the glycoproteins fibronectin and laminin, are elements of the myocardial extracellular matrix (ECM). Alterations in the normal concentrations and ratios of these elements may reflect remodeling in response to physiologic stress. In the case of patients' post-heart transplantation (HTx), specific patterns of alteration may herald myocardial dysfunction. Right ventricular biopsies were taken from the same 28 HTx patients before implantation and 1 week, 2 weeks, and 1, 2, and 3 years after HTx. The above-noted five ECM proteins, six matrix metalloproteinases (MMPs) and two of their tissue inhibitors (TIMPs) were detected by immunohistochemistry and scored as cells per square millimeter or semiquantitatively. The total connective tissue fibers were detected by connective tissue stain and morphometry. Variations in these ECM components were followed in the same patient cohort over 3 years. In summary, during the first 2 weeks after HTx, a predominant increase in connective tissue occurred. Increases in MMP-8 and MMP-9 were found. By 3 years after transplantation, there was a decrease of connective tissue fibers and a significant reduction of all ECM components and an increase in MMPs and TIMPs. These findings may reflect a pattern of remodeling specific to the transplanted heart.
Asunto(s)
Proteínas de la Matriz Extracelular/biosíntesis , Trasplante de Corazón , Metaloproteinasas de la Matriz/biosíntesis , Miocardio/química , Inhibidores Tisulares de Metaloproteinasas/biosíntesis , Adolescente , Adulto , Antígenos CD/biosíntesis , Niño , Femenino , Ventrículos Cardíacos/química , Humanos , Inmunohistoquímica , Masculino , Metaloproteinasa 8 de la Matriz/biosíntesis , Metaloproteinasa 9 de la Matriz/biosíntesis , Persona de Mediana Edad , Factores de TiempoRESUMEN
This study was undertaken to investigate whether there might be differences in the distribution of extracellular matrix (ECM) proteins and matrix metalloproteinases (MMPs), depending on their specific sites within the heart. We investigated 33 explanted human hearts, 15 with dilated cardiomyopathy (DCM) and 18 with ischemic cardiomyopathy (ICM). Transmural samples from the right ventricle, the interventricular septum and the left ventricle, either from near the apex or from near the base were taken from every heart. Frozen sections were processed for connective tissue staining and immunohistochemistry for collagens type I, III, IV, laminin and fibronectin, as well as MMP-1, -2 and -9. Volume densities of laminin in ICM as well as of fibronectin and collagen types I and IV in DCM showed significant differences between right and left ventricular sites. The volume densities of matrix proteins usually did not reveal significant differences among the three left ventricular sites tested in both DCM and ICM. MMPs partly showed differences between the right and the left ventricular myocardium. These results suggest that the distributions of ECM proteins and MMPs differ between the two ventricles in both end-stage DCM and ICM. This gives rise to the hypothesis that a specific pattern of ECM degradation exists in the right and left ventricular myocardium.
Asunto(s)
Cardiomiopatía Dilatada/metabolismo , Proteínas de la Matriz Extracelular/metabolismo , Ventrículos Cardíacos/metabolismo , Metaloproteinasas de la Matriz/metabolismo , Isquemia Miocárdica/metabolismo , Remodelación Ventricular , Cardiomiopatía Dilatada/patología , Femenino , Trasplante de Corazón , Ventrículos Cardíacos/patología , Humanos , Masculino , Persona de Mediana Edad , Isquemia Miocárdica/patologíaRESUMEN
HISTORY AND CLINICAL FINDINGS: A 48-year-old patient from Pakistan was referred with scleral icterus, ascites and lower limb edema. DIAGNOSIS: The patient was suffering from post-hepatitis liver cirrhosis, CHILD stage B, with underlying chronic hepatitis B and C coinfection. TREATMENT AND COURSE: One year after diagnosis, orthotopic liver transplantation (OLT) was performed. To avoid recurrence of the hepatitis B in the transplant, the patient was given a treatment with lamivudine preoperatively. A prophylaxis with a combination of lamivudine and hepatitis B immunoglobulin peri- and postoperatively has been given for more than 5 years. CONCLUSION: The use of lamivudine and hepatitis B immunoglobulin improves transplant survival in patients who have undergone OLT on the basis of post-hepatitis liver cirrhosis secondary to chronic hepatitis B. Costs are reduced by administering the hepatitis B immunoglobulin intramuscularly and by managing the preoperative and aftercare of the patient on an outpatient basis.
Asunto(s)
Antivirales/uso terapéutico , Hepatitis B Crónica/tratamiento farmacológico , Cirrosis Hepática/cirugía , Trasplante de Hígado , Premedicación , Atención Ambulatoria/economía , Hepatitis B Crónica/complicaciones , Hepatitis B Crónica/prevención & control , Hepatitis C Crónica/complicaciones , Humanos , Inmunización Pasiva , Inmunoglobulinas/administración & dosificación , Inmunoglobulinas/uso terapéutico , Inyecciones Intramusculares , Lamivudine/uso terapéutico , Cirrosis Hepática/etiología , Masculino , Persona de Mediana Edad , Cuidados Posoperatorios/economía , Cuidados Preoperatorios/economía , Prevención SecundariaRESUMEN
BACKGROUND: Adenocarcinomas of the small and the large intestine share risk factors and morphological features but both tumor types seem to follow different genetic pathways. The aim of this study on small intestinal carcinomas was to analyze alternative mechanisms of activation of pathways that are typically affected in colorectal cancer. METHODS: Twenty-one sporadic carcinomas were investigated for mutations in KRAS, BRAF, the beta-catenin gene CTNNB1, and the mutational cluster region of APC. Immunohistochemical analysis was performed with a monoclonal antibody for beta-catenin, the transcriptionally active downstream component of wnt signaling. RESULTS: Oncogene mutations were found in 13 (62%) small intestinal adenocarcinomas. Twelve tumors displayed a KRAS mutation, and a novel BRAF mutation at codon 603/604 was seen in one carcinoma without KRAS mutation. One tumor harbored a CTNNB1 mutation consisting of an insertion of 247 nucleotides deriving from chromosome 9. APC mutations were identified in 2 tumors. Immunohistochemistry demonstrated nuclear accumulation of beta-catenin in 5 carcinomas. These carcinomas included the tumor with a CTNNB1 mutation but not those with APC mutations. CONCLUSIONS: Our data show frequent activation of the RAS-RAF-MAPK pathway through mutations of either KRAS or, infrequently, BRAF. Activation of the wnt pathway through accumulation of beta-catenin may have a role in a subset of small intestinal adenocarcinomas but in contrast to colorectal carcinoma, accumulation of beta-catenin is generally not caused by inactivating APC or activating CTNNB1 mutations.
Asunto(s)
Adenocarcinoma/genética , Regulación Neoplásica de la Expresión Génica , Genes ras/genética , Neoplasias Intestinales/genética , Proteínas Quinasas Activadas por Mitógenos/genética , Mutación , Proteínas Proto-Oncogénicas/genética , Quinasas raf/genética , Adenocarcinoma/metabolismo , Anciano , Anciano de 80 o más Años , Proteínas del Citoesqueleto/análisis , Análisis Mutacional de ADN , Femenino , Genes APC , Humanos , Inmunohistoquímica , Neoplasias Intestinales/metabolismo , Masculino , Persona de Mediana Edad , Transactivadores/análisis , Proteínas Wnt , beta CateninaRESUMEN
Anorectal melanomas represent a very small group of mucosal melanoma with unknown etiology and poor prognosis. In view of their location, a history of sun exposure is not likely to have had an impact on their development. Recent epidemiologic data indicate a bimodal age distribution. To date there is no information whether an infection with the human papilloma virus plays a role in the tumorigenesis of anorectal melanoma. The lesions can be misdiagnosed as hemorrhoids on clinical examination. On histological examination amelanotic types have been misdiagnosed as lymphoma, sarcoma,and undifferentiated carcinoma. Useful immunohistochemical markers are S 100 protein, HMB-45, Melan A, and MiTF (microphthalmia-transcription-factor). Therapy includes local excision or abdominoperineal resection followed by optional inguinal and parailiac lymph node dissection, and consecutive chemo- and immunotherapy. The poor long-term prognosis of anorectal melanomas correlates with their advanced tumor size and depth of infiltration at diagnosis. The overall 5-year survival rates range between <5 and 22% in different series.
Asunto(s)
Neoplasias del Ano/epidemiología , Neoplasias del Ano/patología , Melanoma/epidemiología , Melanoma/patología , Neoplasias del Recto/epidemiología , Neoplasias del Recto/patología , Neoplasias del Ano/etiología , Neoplasias del Ano/cirugía , Diagnóstico Diferencial , Humanos , Melanoma/etiología , Melanoma/cirugía , Pronóstico , Neoplasias del Recto/etiología , Neoplasias del Recto/cirugíaRESUMEN
The history of gallbladder involvement by a malignant melanoma in a 65-year-old woman is reported. The gallbladder, clinically resected for cholecystitis, harboured a polypoid dark pigmented tumour. The tumour was identified as a malignant melanoma immunohistochemically by positive reactions for gp100 (HMB45), melan A, and MiTF. Clinically, the patient was treated for cutaneous malignant melanoma by local excision 10 years earlier. The literature of pigmented lesions of the gallbladder is reviewed. In conclusion, the most important differential diagnosis of pigmented lesions of the gallbladder is the secondary gallbladder melanoma.
Asunto(s)
Neoplasias de la Vesícula Biliar/patología , Vesícula Biliar/patología , Melanoma/patología , Neoplasias Primarias Secundarias/patología , Anciano , Colecistitis/cirugía , Diagnóstico Diferencial , Femenino , Humanos , PigmentaciónRESUMEN
Recent morphological and molecular genetic findings have greatly expanded our understanding of gastrointestinal stromal tumors (GISTs). GISTs are now defined by their overexpression of CD117 (KIT), the receptor for the stem cell factor, and can thus be discriminated from smooth muscle tumors. Cytogenetically, GISTs are characterized even in early lesions by frequent entire or partial loss of the chromosomes 14 and 22 and terminal deletions of the chromosomal arm 1p. During tumor progression further chromosomal imbalances accumulate. Following the first report on activating KIT mutations in GISTs, several studies have addressed the role of wild-type and mutant KIT in GISTs and demonstrated activating KIT mutations in the majority of cases. Moreover, KIT tyrosine phosphorylation is even present in KIT mutation-negative GISTs, implicating KIT activation as a central event in the pathogenesis of GISTs. Imatinib (STI571/Glivec) is a selective inhibitor of BCR/ABL, PDGFR and KIT receptor-tyrosine kinases. First therapeutic applications of imatinib in patients with progressive GISTs have yielded promising results. This review focuses on the morphological and molecular findings in GISTs which have opened up a new therapeutic perspective.
Asunto(s)
Neoplasias Gastrointestinales/genética , Neoplasias Gastrointestinales/patología , Neoplasias de los Tejidos Conjuntivo y Blando/genética , Neoplasias de los Tejidos Conjuntivo y Blando/patología , Antígenos CD/genética , Diferenciación Celular , Humanos , Proteínas Proto-Oncogénicas c-kit/genética , Factores de RiesgoRESUMEN
Calnexin (Cnx) has been characterized as a membrane-bound protein that transiently interacts in a unique chaperone system with newly synthesized glycoproteins in order to allow the establishment of their proper tertiary and, in most cases, quarternary structures. The aim of the study was to identify and to locate the expression of Cnx in the three major salivary glands of humans by different methods. Strong expression of Cnx protein and mRNA were generally found in serous salivary secretory units. With regard to mucous secretory units, expression of Cnx was only detectable at a low level in mucous acinar cells of sublingual glands, but not of submandibular glands. Expression of Cnx was always preserved in the surface epithelium of intralobar and interlobular duct segments. In addition, expression of Cnx was detected in sebaceous glands of parotid tissues, with a distribution pattern resembling that seen in sebaceous glands of the normal skin. In conclusion, production of saliva is associated with the expression of Cnx. Synthesis of molecules in mucous secretory units is not necessarily associated with a strong Cnx expression, whereas synthesis in serous secretory units apparently is. The tissue-specific Cnx expression is also paralleled by the observation that the secretions produced by the major salivary glands differ in their composition and amount.
Asunto(s)
Calnexina/biosíntesis , Glándulas Salivales/metabolismo , Western Blotting , Humanos , Inmunohistoquímica , Hibridación in Situ , Glándulas Salivales/citología , Glándulas Sebáceas/citología , Glándulas Sebáceas/metabolismoRESUMEN
BACKGROUND: The causes of Crohn disease (CD) are still regarded as unknown, but impaired mucosal immunoregulation with activation of T-helper-1 (Th-1) cytokine responses is probably involved and may contribute to the morphological changes. We investigated a possible role of osteopontin (Opn) in the pathogenesis of CD. This glycoprotein has been suggested to be involved in the generation of Th-1-type immune responses; moreover, it carries anti-inflammatory activities. METHODS: Ileal samples from CD patients--both actively inflamed and inactive areas as well as unaffected intestinal specimens from controls (normal ileum)--were investigated by Western blot analysis, immunohistochemistry and in situ hybridization. RESULTS: In normal gut, Opn was found to be regularly expressed by plasma cells (CD 38) and a subset of lamina propria mononuclear cells (MNC) as well as by intestinal epithelial cells (IEC). In active CD, immunohistochemistry and in situ hybridization analysis revealed a loss of Opn expression by IEC adjacent to ulcerative lesions, whereas especially plasma cells (CD 38) in the vicinity of such lesions were found to express the molecule. In addition, a slight overexpression of Opn protein was found in metaplastic crypts. However, quantitative analysis of total Opn protein in the ileal mucosa of CD patients did not reveal any difference vis-à-vis control tissues. CONCLUSIONS: The constitutive expression of Opn in normal gut indicates that it is involved in intestinal immune homeostasis. Downregulation of Opn expression in IEC might favour the disintegration of the epithelial barrier. The expression of Opn in lamina propria plasma cells could contribute to disease chronification, probably by affecting cell survival.
Asunto(s)
Enfermedad de Crohn/metabolismo , Íleon/metabolismo , Sialoglicoproteínas/metabolismo , Adulto , Anciano , Western Blotting , Enfermedad de Crohn/patología , Regulación hacia Abajo , Humanos , Ileítis/metabolismo , Ileítis/patología , Íleon/patología , Inmunohistoquímica , Hibridación in Situ , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patología , Persona de Mediana Edad , Osteopontina , ARN Mensajero/análisis , Sialoglicoproteínas/biosíntesisRESUMEN
OBJECTIVE: We asked whether a scoring system [index of pulmonary vascular disease (IPVD)] that quantifies the individual pulmonary vascular pathology would relate to postoperative survival in patients with congenital heart disease and pulmonary hypertension (PH). METHODS: Lung biopsy specimens from 28 patients at a median age of 6 months (1 month to 21 years) were analysed qualitatively and morphometrically. The IPVD and other morphometric parameters were related to haemodynamic findings and survival. RESULTS: Mean pulmonary artery pressure (PAP) was 44 mmHg (15-72 mmHg), and the resistance to pulmonary perfusion was 5 U x m(2) (0.9-14 U x m(2)). There were three early (in-hospital) and three late deaths during the follow-up period of 2.5 years (6 months to 7 years). Incipient plexiform lesions were observed in one infant with trisomy 21 and complete atrioventricular septal defect (cAVSD). An IPVD score above the upper critical limit (>2.2) was not observed during the first year of life. On discriminant analysis, morphometric parameters could not predict mortality ( P=0.08). CONCLUSIONS: The IPVD is not helpful to predict surgical mortality during the first year of life. Patients with trisomy 21 and cAVSD may show advanced pulmonary vascular disease in infancy.
Asunto(s)
Cardiopatías Congénitas/patología , Hemodinámica , Pulmón/patología , Niño , Preescolar , Cardiopatías Congénitas/mortalidad , Cardiopatías Congénitas/cirugía , Humanos , Hipertensión Pulmonar/etiología , Lactante , Pulmón/irrigación sanguínea , Complicaciones Posoperatorias/etiología , Estudios RetrospectivosRESUMEN
Although carcinoma cuniculatum clinically appears to be malignant, histological evaluation often results in a false diagnosis of a benign papillomatous lesion or pseudoepitheliomatous hyperplasia, because the tumor usually displays a well differentiated tissue. In this report morphological and immunohistological features in a rare case of carcinoma cuniculatum of the oral cavity are described. Both clinical features and histomorphological evaluation must be taken into consideration when diagnosing the tumor.
Asunto(s)
Carcinoma/patología , Mucosa Bucal/patología , Neoplasias de la Boca/patología , Papiloma/patología , Anciano , Anciano de 80 o más Años , Carcinoma/diagnóstico , Carcinoma/cirugía , Diagnóstico Diferencial , Humanos , Imagen por Resonancia Magnética , Masculino , Neoplasias de la Boca/diagnóstico , Neoplasias de la Boca/cirugía , Papiloma/diagnóstico , Papiloma/cirugía , Colgajos QuirúrgicosRESUMEN
Deleted in Malignant Brain Tumors 1 (DMBT1) has been proposed as a candidate tumor suppressor gene for brain, lung, and digestive tract cancer. In particular, alterations of the gene and/or a loss of expression have been observed in gastric, colorectal, and esophageal carcinomas. Initial evidence has accumulated that DMBT1 may represent a multifunctional protein. Because the consequences of a loss of DMBT1 function may be different depending on its original function in a particular tissue, we wondered if it is appropriate to assume a uniform role for DMBT1 in digestive tract carcinomas. We hypothesized that a systematic characterization of DMBT1 in the human alimentary tract would be useful to improve the understanding of this molecule and its role in digestive tract carcinomas. Our data indicate that the expression pattern and subcellular distribution of DMBT1 in the human alimentary tract is reminiscent of epithelial mucins. Bovine gallbladder mucin is identified as the DMBT1 homologue in cattle. An elaborate alternative splicing may generate a great variety of DMBT1 isoforms. Monolayered epithelia display transcripts of 6 kb and larger, and generally show a lumenal secretion of DMBT1 indicating a role in mucosal protection. The esophagus is the only tissue displaying an additional smaller transcript of approximately 5 kb. The stratified squamous epithelium of the esophagus is the only epithelium showing a constitutive targeting of DMBT1 to the extracellular matrix (ECM) suggestive of a role in epithelial differentiation. Squamous cell carcinomas of the esophagus show an early loss of DMBT1 expression. In contrast, adenocarcinomas of the esophagus commonly maintain higher DMBT1 expression levels. However, presumably subsequent to a transition from the lumenal secretion to a targeting to the ECM, a loss of DMBT1 expression also takes place in adenocarcinomas. Regarding DMBT1 as a mucin-like molecule is a new perspective that is instructive for its functions and its role in cancer. We conclude that DMBT1 is likely to play a differential role in the genesis of digestive tract carcinomas. However, although DMBT1 originally has divergent functions in monolayered and multilayered epithelia, carcinogenesis possibly converges in a common pathway that requires an inactivation of its functions in the ECM.
Asunto(s)
Aglutininas , Carcinoma de Células Escamosas/metabolismo , Sistema Digestivo/metabolismo , Neoplasias Esofágicas/metabolismo , Receptores de Superficie Celular/fisiología , Adenocarcinoma/genética , Adenocarcinoma/metabolismo , Adulto , Empalme Alternativo , Animales , Northern Blotting , Proteínas de Unión al Calcio , Carcinoma de Células Escamosas/genética , Bovinos , Proteínas de Unión al ADN , Neoplasias Esofágicas/genética , Humanos , Isoformas de Proteínas , ARN Mensajero/genética , ARN Mensajero/metabolismo , Receptores de Superficie Celular/biosíntesis , Receptores de Superficie Celular/genética , Proteínas Supresoras de TumorRESUMEN
We report a Turkish family with parental consanguinity and at risk for sialidosis type II, an inherited autosomal recessive disorder caused by lysosomal alpha-N-acetyl-neuraminidase (sialidase, NEU1) deficiency. The proband was a premature male infant that presented with hydrops, hepatomegaly, respiratory distress syndrome, and anemia and that died of respiratory insufficiency 2 months after birth despite intensive care. An abnormally increased [14C]methylamine incorporation and an isolated deficiency of lysosomal alpha-N-acetyl-neuraminidase were found in cultured skin fibroblasts. A previous pregnancy of the mother terminated in a spontaneous abortion in the 13th week of gestation. A successive pregnancy showed hydrops fetalis, and an enzymatic assay of cultured amniotic fluid cells indicated a deficiency of alpha-N-acetyl-neuraminidase. Following pregnancy termination at 20 weeks gestation, light microscopy of fetal tissues revealed classic vacuolation not only in liver, bone marrow, brain, and kidney, but also in endocrine organs such as the thyroid gland, adrenal gland, hypophysis, and testes, and in the thymus. DNA analysis of the family showed that both the proband and the third sibling had a novel homozygous nonsense point mutation at nucleotide 87 in exon 1 of the alpha-N-acetyl-neuraminidase (neu1) gene causing a substitution of tryptophan at codon 29 by a termination codon (W29X). DNA sequencing of polymerase chain reaction products identified the parents as heterozygous carriers. To detect neu1 mRNA expression, a real-time reverse transcription/polymerase chain reaction was performed, and similar rates of neu1 mRNA expression were found in the fibroblasts of the fetus, the 2nd sibling, and in controls. The very early termination codon with complete loss of neuraminidase activity is probably the molecular basis of the unusually severe vacuolation pattern in this form of congenital sialidosis.
Asunto(s)
Codón sin Sentido/genética , Feto/patología , Enfermedades del Recién Nacido/genética , Enfermedades del Recién Nacido/patología , Neuraminidasa/genética , Diagnóstico Prenatal , Anomalías Múltiples/diagnóstico , Anomalías Múltiples/enzimología , Anomalías Múltiples/genética , Anomalías Múltiples/patología , Aborto Espontáneo/enzimología , Aborto Espontáneo/genética , Aborto Espontáneo/patología , Adulto , Secuencia de Bases , Células Cultivadas , Consanguinidad , Exones/genética , Femenino , Feto/metabolismo , Fibroblastos , Homocigoto , Humanos , Inmunohistoquímica , Lactante , Recién Nacido , Enfermedades del Recién Nacido/diagnóstico , Enfermedades del Recién Nacido/enzimología , Masculino , Neuraminidasa/deficiencia , Neuraminidasa/metabolismo , Mutación Puntual/genética , Embarazo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Turquía , Ultrasonografía PrenatalRESUMEN
We report on a female preterm infant with hepatic failure and neonatal tissue siderosis of hemochromatotic type diagnosed by using both histochemistry and atomic absorption spectroscopy. The infant presented with meconium ileus, signs of rapidly progressive hepatic failure, and hyperferritinemia (7132 ng/ml). Despite surgery and intensive care the infant died 32 days after birth. Postmortem examination showed a wrinkled liver with extensive collapse of the hepatic architecture and regenerating nodules as well as hepatic and extrahepatic iron accumulation of hemochromatotic type, sparing the reticuloendothelial system. Atomic absorption spectroscopy confirmed an increase in the iron content of various organs: liver, heart, pancreas, oral salivary gland, kidney, and adrenal gland. The increase in the iron content of various organs was determined by comparing the analysis of the propositus with those of 5 gestationally age-related preterm infants who had died in the intensive care unit: 2 died of meconium aspiration syndrome, the other 3 of hyaline membrane disease, bronchopulmonary dysplasia, and immaturity, respectively. We also compared the analysis of 15 fetuses having a a condition predisposing to iron accumulation (trisomy 21, trisomy 18, cytomegalovirus, amnion infection syndrome, Rhesus- and ABO-incompatibility, congenital hemolysis, anti-phospholipid syndrome, congenital heart disease). Delta F508, the most frequent mutation seen in cystic fibrosis patients, was excluded by gene sequencing. Different noxae causing iron accumulation in the neonatal period have led to the statement that neonatal hemochromatosis may collect different etiologies, such as metabolic disorders, infections, chromosomal aberrations, and immunological disorders. In this study, we report the singular evidence of neonatal iron accumulation of hemochromatotic type in an infant presenting with meconium ileus and propose a classification of the neonatal disorders associated with iron accumulation.
Asunto(s)
Hemocromatosis/patología , Obstrucción Intestinal/congénito , Fallo Hepático/patología , Siderosis/patología , ADN/análisis , Análisis Mutacional de ADN , Resultado Fatal , Femenino , Hemocromatosis/complicaciones , Hemocromatosis/metabolismo , Histocitoquímica , Humanos , Recién Nacido , Obstrucción Intestinal/complicaciones , Hierro/análisis , Hierro/metabolismo , Fallo Hepático/etiología , Fallo Hepático/metabolismo , Meconio , Reacción del Azul Prusia , Siderosis/complicaciones , Siderosis/metabolismo , Espectrofotometría AtómicaRESUMEN
Cirrhosis is a late stage finding in chronic liver diseases of different aetiology. It is defined morphologically as a diffuse process with the presence of fibrosis and structurally abnormal nodules. The consequences of cirrhosis are both, mechanical and functional. The mechanical complications result from intra- and extrahepatic shunting of blood and portal hypertension while the functional relevance bases upon a failure of liver cells to perform their physiological role in metabolism, synthesis and secretion. Beside these complications that are directly linked to liver function cirrhosis in itself is a risk factor for hepatocellular carcinoma.
Asunto(s)
Hipertensión Portal/patología , Cirrosis Hepática/patología , Carcinoma Hepatocelular/patología , Transformación Celular Neoplásica/patología , Humanos , Hipertensión Portal/etiología , Hígado/patología , Cirrosis Hepática/etiología , Neoplasias Hepáticas/patologíaRESUMEN
OBJECTIVE: The development of accelerated graft arteriosclerosis is a major cause of late death after orthotopic heart transplantation. The influence and the extent of peritransplant injury, especially of cardiomyocyte or capillary endothelial cell edema is discussed. METHODS: A morphometric ultrastructural analysis of myocardial biopsies from 29 donor hearts (21 male, age 34+/-11 years) was performed. Right ventricular biopsies were obtained before cardioplegia (A), immediately following cardioplegia (B) (Custodiol, Dr. F. Köhler Chemie GmbH, Alsbach-Hähnlein, Germany), before implantation (C), after 30 (D) or 60 (E) min of reperfusion and 1 week after transplantation (F). Mean ischemic time was 185+/-68 min. Quantitative electron microscopy was carried out in five samples per heart and time point and in 30 test fields per sample by 'random systematic sampling' and 'point and intersection counting'. As parameters for cell edema the volume density of myofibrils in cardiomyocytes and the mean barrier thickness of capillary endothelia were analyzed. P-values of less than 0.05 were regarded as significant. Significant differences in contrast to the previous values are marked by *. RESULTS: The volume density of myofibrils (vol.%) was as follows: (B) 63.6+/-3.2, (C) 61.8+/-3.2, (D) 62.9+/-3.2, (E) 63.6+/-4.5. The mean barrier thickness (nm) was as follows: (A) 353+/-21, (B) 376+/-59, (C) 416+/-71*, (D) 473+/-45*; (E) 453+/-50*, (F) 379+/-39. CONCLUSIONS: Apart from a generally accepted edema of cardiomyocytes a relevant capillary endothelial cell edema develops during clinical heart transplantation. In contrast to cardiomyocytes the cell edema of endothelia shows a more pronounced and significant progression during cold ischemia and early reperfusion. After 60 min of reperfusion it is still significantly more pronounced than at the onset of ischemia. After 1 week there are no statistical differences compared to the initial values. Thus, an edema of capillary endothelia probably will trigger inhomogeneities in capillary perfusion. Peritransplant injury of endothelia may contribute to the later development of accelerated allograft arteriosclerosis.
Asunto(s)
Endotelio Vascular/diagnóstico por imagen , Trasplante de Corazón , Daño por Reperfusión Miocárdica/diagnóstico por imagen , Miocardio/ultraestructura , Adulto , Edema/patología , Femenino , Paro Cardíaco Inducido , Humanos , Masculino , Persona de Mediana Edad , Miofibrillas/ultraestructura , UltrasonografíaRESUMEN
Crohn's disease (CD) is a chronic inflammatory intestinal disorder with disturbance and injury of the intestinal mucosal barrier, in which various proinflammatory molecules as well as molecules with antiinflammatory activity and cytoprotective function are found to be expressed. We investigated whether clusterin, a multifunctional cytoprotective protein, is upregulated in Crohn's disease, because augmented expression of clusterin is seen in many organs following various forms of tissue injury. Human actively and inactively inflamed ileal tissues from CD patients as well as normal intestinal specimens from control patients (normal ileum) were investigated by Western blot analysis, immunohistochemisty and in situ hybridization. As compared with controls, a strongly enhanced expression of clusterin was found in CD tissues, correlating with disease activity. Immunohistochemistry and in situ hybridization analysis revealed foci of crypts almost completely lined by clusterin expressing enterocytes in CD, a feature that was never seen in controls. Such crypts appeared especially within the morphologically intact mucosa apart from erosive or ulcerative lesions. Besides epithelia, clusterin was also expressed by inflammatory mononuclear cells. Enhanced expression of clusterin by crypt epithelia might reflect a cytoprotective function of the protein in order to prevent further injury of the intestinal mucosal barrier in CD.
Asunto(s)
Enfermedad de Crohn/metabolismo , Glicoproteínas/metabolismo , Íleon/metabolismo , Chaperonas Moleculares/metabolismo , Adulto , Anciano , Western Blotting , Estudios de Casos y Controles , Clusterina , Enfermedad de Crohn/genética , Enfermedad de Crohn/patología , Glicoproteínas/genética , Humanos , Íleon/patología , Inmunohistoquímica , Hibridación in Situ , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patología , Persona de Mediana Edad , Chaperonas Moleculares/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Regulación hacia ArribaRESUMEN
Sialidosis is a lysosomal storage disease caused by the deficiency of alpha-N-acetylneuraminidase (NEU1; sialidase), the key enzyme for the intralysosomal catabolism of sialylated glycoconjugates. We have identified a homozygous transversion in the last intron (IVSE +1 G>C) in neu1 of a sialidosis patient. Sequencing of the truncated cDNA revealed an alternatively spliced neu1 transcript which lacks the complete sequence of exon 5. Skipping of exon 5 leads to a frameshift and results in a premature termination codon. This is the first description of an intronic point mutation causing a complete deficiency of the lysosomal neuraminidase activity.
Asunto(s)
Lisosomas/enzimología , Mucolipidosis/genética , Neuraminidasa/genética , Sitios de Empalme de ARN/genética , Secuencia de Aminoácidos , Exones/genética , Fibroblastos/enzimología , Fibroblastos/metabolismo , Eliminación de Gen , Humanos , Datos de Secuencia Molecular , Mucolipidosis/enzimología , Mucolipidosis/patología , Mutación , Neuraminidasa/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de AminoácidoRESUMEN
INTRODUCTION: Since the first description of two children affected with human immunodeficiency virus (HIV), various diagnostic procedures have been established. However, the morphologic study of biopsy and autopsy material from children with acquired immunodeficiency syndrome (AIDS) is still of fundamental importance. The morphology has contributed remarkably to the progress in understanding the pathogenesis of the primary tissue lesions and of the sequelae directly or indirectly associated with HIV infection. The aim of this study was to evaluate the HIV pathology in pediatric AIDS (PAIDS) through a systematic review of the English-language literature and to draw up a practical checklist protocol for the postmortem. MATERIALS AND METHODS: Information on HIV pathology in childhood was retrieved from a MEDLINE search (January 1994-January 2001) of the original reports and bibliographic article reviews published in English. Citations from papers retrieved were screened and retrieved papers were evaluated. RESULTS: Based on the screened data, we propose a practical, organ-oriented checklist protocol for the postmortem according to the HIV pathogenesis. CONCLUSIONS: Evidence-based medicine is a paradigm now exerting increasing influence in related fields such as surgery, general practice, psychiatry, and pathology. This article is a summary of the literature on PAIDS pathology. The protocol that we propose is particularly useful for pediatric pathology programs and for electronic data processing.
Asunto(s)
Autopsia/métodos , Infecciones por VIH/patología , MEDLINE , Síndrome de Inmunodeficiencia Adquirida/patología , Médula Ósea/patología , Sistema Cardiovascular/patología , Niño , Sistema Digestivo/patología , Procesamiento Automatizado de Datos , Sistema Endocrino/patología , Femenino , Humanos , Riñón/patología , Pulmón/patología , Ganglios Linfáticos/patología , Masculino , Sistema Nervioso/patología , Especificidad de Órganos , Revisiones Sistemáticas como AsuntoRESUMEN
Desmosis coli is a disturbance of the intramural connective tissue mesh network of the colonic wall which can lead to a hypoperistalsis syndrome with chronic constipation in the absence of any anomaly of the vegetative gut innervation. The condition typically occurs in infants and adolescents; however, as an incomplete form, desmosis coli can also cause chronic constipation in adults, as demonstrated in this case report.