RESUMEN
The response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is largely impacted by the level of virus exposure and status of the host immunity. The nature of protection shown by direct asymptomatic contacts of coronavirus disease 2019 (COVID-19)-positive patients is quite intriguing. In this study, we have characterized the antibody titer, SARS-CoV-2 surrogate virus neutralization, cytokine levels, single-cell T-cell receptor (TCR), and B-cell receptor (BCR) profiling in asymptomatic direct contacts, infected cases, and controls. We observed significant increase in antibodies with neutralizing amplitude in asymptomatic contacts along with cytokines such as Eotaxin, granulocyte-colony stimulating factor (G-CSF), interleukin 7 (IL-7), migration inhibitory factor (MIF), and macrophage inflammatory protein-1α (MIP-1α). Upon single-cell RNA (scRNA) sequencing, we explored the dynamics of the adaptive immune response in few representative asymptomatic close contacts and COVID-19-infected patients. We reported direct asymptomatic contacts to have decreased CD4+ naive T cells with concomitant increase in CD4+ memory and CD8+ Temra cells along with expanded clonotypes compared to infected patients. Noticeable proportions of class switched memory B cells were also observed in them. Overall, these findings gave an insight into the nature of protection in asymptomatic contacts.
Asunto(s)
Inmunidad Adaptativa/inmunología , COVID-19/inmunología , Genómica/métodos , SARS-CoV-2/inmunología , Análisis de la Célula Individual/métodos , Inmunidad Adaptativa/genética , Adulto , Anticuerpos Antivirales/inmunología , COVID-19/genética , COVID-19/virología , Citocinas/inmunología , Citocinas/metabolismo , Femenino , Perfilación de la Expresión Génica/métodos , Humanos , Masculino , Células B de Memoria/inmunología , Células B de Memoria/metabolismo , Células B de Memoria/virología , Persona de Mediana Edad , SARS-CoV-2/fisiología , Análisis de Secuencia de ARN/métodos , Linfocitos T/inmunología , Linfocitos T/metabolismo , Linfocitos T/virología , Adulto JovenRESUMEN
Water scarcity and salinity are major challenges facing agriculture today, which can be addressed by engineering plants to grow in the boundless seawater. Understanding the mangrove plants at the molecular level will be necessary for developing such highly salt-tolerant agricultural crops. With this objective, we sequenced the genome of a salt-secreting and extraordinarily salt-tolerant mangrove species, Avicennia marina, that grows optimally in 75% seawater and tolerates >250% seawater. Our reference-grade ~457 Mb genome contains 31 scaffolds corresponding to its chromosomes. We identified 31,477 protein-coding genes and a salinome consisting of 3246 salinity-responsive genes and homologs of 614 experimentally validated salinity tolerance genes. The salinome provides a strong foundation to understand the molecular mechanisms of salinity tolerance in plants and breeding crops suitable for seawater farming.
Asunto(s)
Avicennia/genética , Genoma de Planta/genética , Tolerancia a la Sal/genética , Sales (Química)/metabolismo , Agricultura/métodos , Avicennia/metabolismo , ADN de Plantas/química , ADN de Plantas/genética , Perfilación de la Expresión Génica/métodos , Regulación de la Expresión Génica de las Plantas , Tamaño del Genoma/genética , Genómica/métodos , RNA-Seq/métodos , Salinidad , Agua de Mar , Análisis de Secuencia de ADN/métodosRESUMEN
Heavy metal pollution of agricultural soils is one of the most severe ecological problems in the world. Prosopis juliflora, a phreatophytic tree species, grows well in heavy metal laden industrial sites and is known to accumulate heavy metals. Heavy Metal ATPases (HMAs) are ATP driven heavy metal pumps that translocate heavy metals across biological membranes thus helping the plant in heavy metal tolerance and phytoremediation. In the present study we have isolated and characterized a novel 28.9 kDa heavy metal ATPase peptide (PjHMT) from P. juliflora which shows high similarity to the C-terminal region of P1B ATPase HMA1. It also shows the absence of the invariant signature sequence DKTGT, and the metal binding CPX motif but the presence of conserved regions like MVGEGINDAPAL (ATP binding consensus sequence), HEGGTLLVCLNS (metal binding domain) and MLTGD, GEGIND and HEGG motifs which play important roles in metal transport or ATP binding. PjHMT, was found to be upregulated under cadmium and zinc stress. Heterologous expression of PjHMT in yeast showed a higher accumulation and tolerance of heavy metals in yeast. Further, transgenic tobacco plants constitutively expressing PjHMT also showed increased accumulation and tolerance to cadmium. Thus, this study suggests that the transport peptide from P. juliflora may have an important role in Cd uptake and thus in phytoremediation.
Asunto(s)
Adenosina Trifosfatasas/genética , Metales Pesados/metabolismo , Nicotiana/genética , Prosopis/genética , Secuencia de Aminoácidos/genética , Cadmio/metabolismo , Cadmio/toxicidad , Regulación de la Expresión Génica de las Plantas , Metales Pesados/toxicidad , Péptidos/genética , Raíces de Plantas/genética , Raíces de Plantas/crecimiento & desarrollo , Plantas Modificadas Genéticamente/genética , Plantas Modificadas Genéticamente/crecimiento & desarrollo , Prosopis/efectos de los fármacos , Prosopis/crecimiento & desarrollo , Suelo/química , Nicotiana/efectos de los fármacos , Nicotiana/crecimiento & desarrollo , Zinc/metabolismo , Zinc/toxicidadAsunto(s)
Ferritinas/genética , Regulación de la Expresión Génica de las Plantas , Hierro/análisis , Oryza/genética , Plantas Modificadas Genéticamente/metabolismo , Ferritinas/metabolismo , Histocitoquímica , Oryza/metabolismo , Plantas Modificadas Genéticamente/química , Plantas Modificadas Genéticamente/genéticaRESUMEN
Normal growth and development of plants is greatly dependent on the capacity to overcome environmental stresses. Environmental stress conditions like high salinity, drought, high incident light and low or high temperature cause major crop losses worldwide. A common denominator in all these adverse conditions is the production of reactive oxygen species (ROS) within different cellular compartments of the plant cell. Plants have developed robust mechanisms including enzymatic or nonenzymatic scavenging pathways to counter the deleterious effects of ROS production. There are a number of general reviews on oxidative stress in plants and few on the role of ROS scavengers during stress conditions. Here we review the regulation of antioxidant enzymes during salt stress in halophytes, especially mangroves. We conclude that (i) antioxidant enzymes protect halophytes from deleterious ROS production during salt stress, and (ii) genetic information from mangroves and other halophytes would be helpful in defining the roles of individual isoforms. This information would be critical in using the appropriate genes for oxidative stress defence for genetic engineering of enhanced stress tolerance in crop systems.
Asunto(s)
Antioxidantes/metabolismo , Avicennia/metabolismo , Estrés Oxidativo/fisiología , Adaptación Fisiológica/efectos de los fármacos , Avicennia/efectos de los fármacos , Avicennia/enzimología , Avicennia/genética , Catalasa/metabolismo , Ferritinas/metabolismo , Genes de Plantas , Estrés Oxidativo/efectos de los fármacos , Estrés Oxidativo/genética , Peroxidasa/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Cloruro de Sodio/farmacología , Superóxido Dismutasa/metabolismoRESUMEN
Salinization poses an increasingly serious problem in coastal and agricultural areas with negative effects on plant productivity and yield. Avicennia marina is a pantropical mangrove species that can survive in highly saline conditions. As a first step towards the characterization of genes that contribute to combating salinity stress, the construction of a cDNA library of A. marina genes is reported here. Random expressed sequence tag (EST) sequencing of 1,841 clones produced 1,602 quality reads. These clones were classified into functional categories, and BLAST: comparisons revealed that 113 clones were homologous to genes earlier implicated in stress responses, of which the dehydrins are the most predominant in this category. Of the ESTs analyzed, 30% showed homology to previously uncharacterized genes in the public plant databases. Of these 30%, 52 clones were selected for reverse Northern analysis: 26 were shown to be up-regulated and five shown to be down-regulated. The results obtained by reverse Northern analysis were confirmed by Northern analysis for three clones.