Bioassessment of Cd and Pb at Multiple Growth Stages of Wheat Grown in Texturally Different Soils Using Diffusive Gradients in Thin Films and Traditional Extractants: A Comparative Study.

The bioavailability of heavy metals in soil is a crucial factor in determining their potential uptake by plants and their subsequent entry into the food chain. Various methods, including traditional chemical extractants and the diffusive gradients in thin films (DGT) technique, are employed to assess this bioavailability. The bioavailability of heavy metals, particularly cadmium (Cd) and lead (Pb), is also influenced by soil texture and their concentrations in the soil solution. The primary objectives of this experiment were to compare and correlate the assessment of the Cd and Pb bioavailability using the DGT technique and traditional extractants across two soil textural classes: sandy clay loam (SCL) and clay loam (CL) at two contamination levels: aged contaminated (NC) and artificially contaminated (AC). The specific objectives included assessing the bioavailability of Cd and Pb at different growth stages of the wheat plant and correlating the DGT-based bioassessments of Cd and Pb with their concentrations in various plant parts at different growth stages. This study also compared the effectiveness of the DGT method and traditional extraction techniques in assessing the bioavailable fractions of Cd and Pb in soil. The regression analysis demonstrated strong positive correlations between the DGT method and various extraction methods. The results showed that the wheat plants grown in the AC soils exhibited lower root, shoot, and grain weights compared to those grown in the NC soils, indicating that metal contamination negatively impacts plant performance. The concentrations of Cd and Pb in the wheat tissues varied across different growth stages, with the highest levels observed during the grain filling (S3) and maturity (S4) stages. It is concluded that the in situ assessment of Cd and Pb though DGT was strongly and positively correlated with the Cd and Pb concentration in wheat plant parts at the maturity stage. A correlation and regression analysis of the DGT assessment and traditional extractants showed that the DGT method provides a reliable tool for assessing the bioavailability of Cd and Pb in soils and helped in developing sustainable soil management strategies to ensure the safety of agricultural products for human consumption.

Molasses-based waste water irrigation: a friend or foe for carrot (Daucus carota L.) growth, yield and nutritional quality.

Management of molasses-based wastewater generated in yeast and sugar industries is a major environmental concern due to its high chemical oxygen demand and other recalcitrant substances. Several strategies have been used to reduce the inland discharge of wastewater but the results are not satisfactory due to high operating cost. However, reuse of molasses-based wastewater irrigation in agriculture has been a major interest nowadays to reduce the freshwater consumption. Thus, it is crucial to monitor the impacts of molasses-based waste water irrigation on growth, metabolism, yield and nutritional quality of crops for safer consumer's health. In present study, carrot seeds of a local cultivar (T-29) were germinated on filter paper in Petri dishes under controlled conditions. The germinated seeds were then transplanted into pots and irrigated with three different treatments normal water (T0), diluted molasses-based wastewater (T1), and untreated molasses-based wastewater (T2), in six replicates. Results revealed that carrot irrigated with untreated molasses-based waste water had exhibited significant reductions in growth, yield, physiology, metabolism, and nutritional contents. Additionally, accumulation of Cd and Pb contents in carrot roots irrigated with untreated molasses-based waste water exceed the permissible limits suggested by WHO and their consumption may cause health risks. While, diluted molasses-based waste water irrigation positively enhanced the growth, yield of carrot plants without affecting the nutritional quality. This strategy is cost effective, appeared as most appropriate alternative mean to reduce the freshwater consumption in water deficit regions of the world.

Investigating the combined effects of ß-sitosterol and biochar on nutritional value and drought tolerance in Phaseolus vulgaris under drought stress.

Climate change-induced drought stress decreases crop productivity, but the application of ß-sitosterol (BS) and biochar (BC) boosts crop growth and yield. A pot experiment was conducted to examine the effects of the alone and combined application of BS and BC on the growth and yield of Phaseolus vulgaris under drought stress. The synergistic application of BS and BC increased plant height (46.9cm), shoot dry weight (6.9g/pot), and root dry weight (2.5g/pot) of P. vulgaris plants under drought stress. The trend of applied treatments for photosynthetic rate remained as BC (15%)

Plasma-Based Modification of Tin Halide Perovskite Interfaces for Photovoltaic Applications.

Tin halide perovskites represent the most suitable alternative to their lead-based counterparts for sustainable photovoltaics. One of the most important drawbacks of this class of materials is the intrinsic tendency of tin (II) to oxidize under certain conditions and as a consequence of aging. Here, we explore plasma processing to gently treat the surface of the tin perovskite films. As shown by chemical, optical, and morphological analyses, this treatment by generating transient active species on the surface of the material impacts its aging, inhibiting the tendency of tin (II) to oxidize. Plasma-treated stored devices show a power conversion efficiency slightly higher and narrower in the distribution than that of the reference devices. The positive impact of this noninvasive technique, which can be easily implemented in large-area manufacturing facilities, increases the potential of lead-free alternative perovskite photovoltaics.

Microbial-inoculated biochar for remediation of salt and heavy metal contaminated soils.

Numerous harmful contaminants (i.e. salt and heavy metals) have become major threats to soil and are being introduced into the soil through human and geological activities. These contaminants are raising global concerns about their toxic effects on food safety, human health and reclamation mechanisms. Microbial-inoculated biochar can improve soil environment by immobilizing and transforming contaminants in soil and altering the physico-chemical and biochemical properties of soil. In this review we will discuss the positive effects of microbial-modified biochar on physicochemical properties of contaminated soil. It can decrease the pH, EC while increase CEC, OM and other biochemical properties of soil. Additionally, we discuss the efficacy of biochar as a microbial carrier for salt and heavy metals-contaminated soil and plant growth in those soils. This review provides a better understanding of the potential of microbial biochar can be used for bioremediation of contaminated soil, which will help the researcher to modify biochar in a targeted way for specific applications.

Probiotic set yogurt infused with orange pulp: A multifaceted evaluation of nutritional, antioxidant, and physical attributes.

Fortification of yogurt with orange pulp tends to increase its protein network strength resulting in reduced syneresis. The aim of the current study was to prepare set-type orange yogurt with cow milk, skim milk powder, guar gum, and orange pulp at 0%, 1.0%, 2.0%, 3.0%, and 4.0% concentrations, respectively. The changes in proximate, total soluble solid, antioxidant activity, ascorbic acid, and syneresis were assessed. Yogurt was stored for consecutive three weeks during that duration all attributes were evaluated weekly. Set-type orange pulp incorporated yogurt significantly increased the fat (3.91% to 4.9%), protein (3.90% to 3.94%), moisture (84% to 84.80%), total soluble solids (16.01% to 18.51%), ascorbic acid (16.99% to 20.43%), and syneresis (28.90% to 29.94%), respectively. Overall results indicate that 4% orange pulp-enriched set-type yogurt presented more stable parameters as compared to other formulas.

Optimization of Extraction Conditions for Biological Attributes of Newly Developed NARC-G1 Garlic Using Response Surface Methodology and Its GC-MS Characterization.

The present paper is a comprehensive study on the optimization of ultrasonic assisted extraction and characterization of valued components from the newly developed garlic cultivar NARC-G1. The response surface methodology was employed to optimize the extraction time, solvent-to-solid ratio and extraction temperature, for optimal total phenolic contents (TPC), DPPH radical scavenging activity, α-amylase inhibition, and α-glucosidase inhibition. Under the optimized conditions (23 min extraction time, 60 °C temperature, and 39 : 1 solvent-to-solute ratio) the optimal TPC (13.76 mg GAE/g), DPPH radical scavenging activity (62.76 %), α-amylase inhibition (71.43 %), and α-glucosidase inhibition (79.43 %) were obtained. Significant correlations were observed between actual values and predicted values for the selected responses. Gas chromatography-mass spectrometry (GC-MS), revealed 26 high-value bioactives, with diallyl disulphide being the most abundant (16.22 %), followed by 2,3-dihydro-3,5-dihydroxy-6-methyl, 4H-pyran-4-one and 5-Hydroxymethylfurfural. Additionally, in silico investigation indicated that stigmasterol, arbutin, squalene, α-tocopherol and linoleic acid were responsible for antihyperglycemic potential. Moreover, antimicrobial activity revealed that S. mutans (19.25±1.98 mm) and L. monocytogenes (19.34±2.04 mm) were the most sensitive, while P. aeruginosa was the least sensitive bacterial strains against the optimized NARC-G1 garlic extract. This research effectively explains the optimal recovery of high-valued components from NARC-G1 and may contribute to nutra-pharmaceutical and functional food developments.

Initial Experience of Pancreaticoduodenectomy in a Newly Developed Hepato-Pancreato-Biliary Unit Serving in a Lower-Middle-Income Country.

Introduction: Pancreaticoduodenectomy (PD) is the only potentially curative treatment for pancreatic head adenocarcinoma. This study aimed to determine the short-term outcomes of PD performed over 1 year at a newly established hepato-pancreatico-biliary unit in Khyber Pakhtunkhwa province of Pakistan. Material and Methods: A retrospective analysis of a prospectively maintained hospital information system (HIS) was undertaken of all patients referred to the unit between May 2021 and August 2022. Data were collected from the medical records of patients in the HIS. Data were analyzed for primary location, age, complications, and operative parameters. Results: The primary sites of disease were ampulla (n = 18, 52.9%), pancreas (n = 11, 32.4%), and duodenum (n = 5, 14.7%). The median duration of surgery was 7 h. 16 (47.1%) patients required blood transfusion either intraoperatively or in the perioperative period. Patients with pre-operative biliary drainage (PBD) were more likely to have multidrug-resistant positive bile cultures with a P-value of 0.2 (n = 12 [35.3%] vs. n = 5 [14.7%]). Overall morbidity was 38.2%. The most common complications were wound infection (n = 12, 35.3%), delayed gastric emptying (n = 6, 17.6%), and type B pancreatic fistula (n = 3, 8.8%). The complication rate was higher in patients with biliary stenting (n = 11 [32.4%] vs. n = 2 [5.9%]; P = 0.06). The median length of hospital stay for patients without complications was less (6 vs. 12 days; P < 0.001). The complication rate was lower in total laparoscopic PD (TLPD) with P = 0.4 (TLPD: 2.9%, open: 23.5%, laparoscopic assisted: 11.8%). 90-day mortality was zero. Conclusion: Short-term outcomes for PD in our facility are comparable to high-volume centers. PBD can significantly increase operative time, hospital stay, and morbidity.

Development of hybrid polyvinylpyrrolidone/carboxymethyl cellulose/collagen incorporated oregano scaffolds via direct ink write printing for potential wound healing applications.

Additive manufacturing can develop regenerative scaffolds for wound healing. 3D printing offers meticulous porosity, mechanical integrity, cell adhesion and cost-effectiveness. Herein, we prepared ink composed of carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), collagen, and oregano extract for the fabrication of tissue constructs. The blend was optimized to form a homogeneous ink and rheological characterization demonstrated shear thinning behavior. The scaffolds were printed using Direct Ink Write (DIW) at a flow speed of 4 mm3/s and a layer height of 0.18 mm. The fabricated scaffolds demonstrated an ultimate tensile strength (UTS) and toughness of 730 KPa and 2.72 MJ/m3, respectively. Scanning Electron Microscopy (SEM) revealed an average pore size of 300 ± 30 µm. Fourier transform infrared spectroscopy (FTIR) analysis confirmed that all materials were present. The contact angle of the composite scaffold was 68° ± 1°. Moreover, the scaffolds presented 82 % mass loss (degradation) in phosphate buffer saline (PBS) over 14 days. The composite scaffold exhibited inhibition zones of 9 mm and 12 mm against Staphylococcus aureus and Escherichia coli, respectively. The PVP/CMC/collagen/oregano 3D printed scaffolds exhibited excellent biocompatibility with the mesenchymal stem cells and humman dermal fibroblast cells, confirmed by water-soluble tetrazolium - 8 (WST-8) assay (test conducted for 7 days). The enhanced angiogenic potential of said scaffold was assesed by release of vascular endothelial growth factor followed by further validation through in-vivo CAM assay. Thus, confirming suitability for the potential wound healing application.

Electrophoretically deposited Asphaltum punjabianum (Shilajit) coatings on polyvinylalcohol/carboxymethylcellulose hydrogels.

The present study aims to develop Asphaltum punjabianum (namely Shilajit) coated Polyvinyl alcohol (PVA)/Carboxymethyl cellulose (CMC) hydrogels and examine their structural, morphological, degradation, and biological properties. Hydrogels were produced at two different concentrations: 70:30 PVA/CMC and 90:10 PVA/CMC. Following that, Shilajit was applied to the synthesized hydrogels using electrophoretic deposition for a duration of 3 min at 30 V. The scanning electron microscopy images showed that the hydrogel's surface had a regular distribution of irregular Shilajit particles. Fourier transform infrared spectroscopy (FTIR) analysis demonstrated the presence of hydrogen bonding between PVA and CMC hydrogels and Shilajit, indicating the successful deposition of Shilajit on the hydrogel. The hydrogels coated with Shilajit exhibited strong antimicrobial activity, resulting in an inhibition zone measuring 34 mm against Escherichia coli (E. coli) and 41 mm against Staphylococcus aureus (S. aureus). The hydrogels exhibited a cell viability of 80 % with mesenchymal stem cells (MSCs), and the release of collagen II also increased. Furthermore, the PVA/CMC/Shilajit hydrogel exhibited a lower degradation rate compared to the PVA/CMC hydrogel. The results of the swelling, degradation, and drug release studies indicate that the shilajit coating is appropriate for the long-term process of tissue and cartilage regeneration.

Advanced Sensing System for Sleep Bruxism across Multiple Postures via EMG and Machine Learning.

Diagnosis of bruxism is challenging because not all contractions of the masticatory muscles can be classified as bruxism. Conventional methods for sleep bruxism detection vary in effectiveness. Some provide objective data through EMG, ECG, or EEG; others, such as dental implants, are less accessible for daily practice. These methods have targeted the masseter as the key muscle for bruxism detection. However, it is important to consider that the temporalis muscle is also active during bruxism among masticatory muscles. Moreover, studies have predominantly examined sleep bruxism in the supine position, but other anatomical positions are also associated with sleep. In this research, we have collected EMG data to detect the maximum voluntary contraction of the temporalis and masseter muscles in three primary anatomical positions associated with sleep, i.e., supine and left and right lateral recumbent positions. A total of 10 time domain features were extracted, and six machine learning classifiers were compared, with random forest outperforming others. The models achieved better accuracies in the detection of sleep bruxism with the temporalis muscle. An accuracy of 93.33% was specifically found for the left lateral recumbent position among the specified anatomical positions. These results indicate a promising direction of machine learning in clinical applications, facilitating enhanced diagnosis and management of sleep bruxism.

Potential magnetic drug targeting with magnetite nanoparticles in cancer treatment by enhancer-modifier natural herb and loaded drug.

In the present study, we prepared magnetite nanoparticles (MNPs) loaded with natural Moringa oleifera (M. olf) herb and Epilim (Ep) drug to evaluate the anti-cancerous activity against brain cancer cells. All the samples were prepared via co-precipitation approach modified with different concentrations of M. olf and Ep drug at room temperature. The MNPs loaded with drug and natural herb were studied in terms of crystal structure, morphology, colloidal stability, size distribution, and magnetic properties. Field emission scanning electron microscopy (FESEM) images exhibited the morphologies of samples with spherical shape as well as the particles size of 9 nm for MNPs and up to 23 nm for its composites. The results of vibrating sample magnetometer (VSM) indicated the magnetization saturation (Ms) of 42.510 emu/g for MNPs. This value reduced to 16-35 emu/g upon loading MNPs with different concentrations of M. olf and Ep. Fourier transform infrared spectroscopy (FTIR) indicated the chemical interaction between the Ep, M.olf and MNPs. Brunauer-Emmett-Teller (BET) analysis confirmed the largest surface area for MNPs (422.61 m2/g) which gradually reduced on addition of M. olf and Ep indicating the successful loading. The zeta potential measurements indicated that the MNPs and MNPs loaded with M. olf and Ep are negatively charged and can be dispersed in the suspension. Furthermore, U87 human glioblastoma cell line was used for the in vitro cellular studies to determine the efficacy of synthesized MNPs against cancer cells. The results confirmed the anti-proliferative activity of the MNPs loaded with M. olf and Ep.

Gelatin Methacryloyl (GelMA) - 45S5 Bioactive Glass (BG) Composites for Bone Tissue Engineering: 3D Extrusion Printability and Cytocompatibility Assessment Using Human Osteoblasts.

3D extrusion printing has been widely investigated for low-volume production of complex-shaped scaffolds for tissue regeneration. Gelatin methacryloyl (GelMA) is used as a baseline material for the synthesis of biomaterial inks, often with organic/inorganic fillers, to obtain a balance between good printability and biophysical properties. The present study demonstrates how 45S5 bioactive glass (BG) addition and GelMA concentrations can be tailored to develop GelMA composite scaffolds with good printability and buildability. The experimental results suggest that 45S5 BG addition consistently decreases the compression stiffness, irrespective of GelMA concentration, albeit within 20% of the baseline scaffold (without 45S5 BG). The optimal addition of 2 wt % 45S5 BG in 7.5 wt % GelMA was demonstrated to provide the best combination of printability and buildability in the 3D extrusion printing route. The degradation decreases and the swelling kinetics increases with 45S5 BG addition, irrespective of GelMA concentration. Importantly, the dissolution in simulated body fluid over 3 weeks clearly promoted the nucleation and growth of crystalline calcium phosphate particles, indicating the potential of GelMA-45S5 BG to promote biomineralization. The cytocompatibility assessment using human osteoblasts could demonstrate uncompromised cell proliferation or osteogenic marker expression over 21 days in culture for 3D printable 7.5 wt % GelMA -2 wt % 45S5 BG scaffolds when compared to 7.5 wt % GelMA. The results thus encourage further investigations of the GelMA/45S5 BG composite system for bone tissue engineering applications.

Occurrence, spatial distribution and ecological risk assessment of Organophosphate Esters in surface water and sediments from the Ravi River and its tributaries.

Organophosphate esters (OPEs) are widely used as substitutes for brominated flame retardants and characterized as emerging contaminants. Due to their toxicity and persistent nature, OPEs are becoming a matter of greater concern worldwide. However, information about the pollution profile of OPEs and associated ecological risks is still scarce in environmental matrices of the South Asian region, particularly Pakistan. Hence, the current study was conducted to investigate the occurrence, spatial distribution patterns, ecological risks and riverine flux of 10 organophosphate esters in surface water and sediments of Ravi River and its four tributaries. The concentrations of ∑10OPEs were in the range of 19.2 - 105 ng/L, with the dominance of chlorinated-OPEs (51 %) in surface water, whereas in case of sediments, the ∑10OPEs concentrations ranged from 20.7 to 149 ng/g dw, with high abundance of non - chlorinated alkyl-OPEs, which contributed about 56 % to total OPE concentration. The correlation analysis signified a strong positive relation of OPEs with TOC (p < 0.05, R = 0.76) in sediments; and in addition to this, field-based LogKoc values were estimated to be higher than predicted LogKoc. Moreover, a significantly positive correlation (p < 0.05, R = 0.88) was observed between LogKoc and LogKow, implying that hydrophobicity plays a significant role in OPE distribution in different environmental matrices. The global comparison revealed that contamination status of OPEs in the present study was comparatively lower than other regional findings, furthermore, principal component analysis suggested vehicular emissions, industrial discharges, household supplies and atmospheric deposition as main sources of OPEs occurrence in current study region. Furthermore, the riverine flux of ∑10OPEs was estimated to be 0.68 tons/yr and the ecological risk assessment indicated that all OPEs, except EHDPP and TCrP, showed negligible or insignificant ecological risks for aquatic organisms.

An outbreak of Providencia rettgeri bacteremia at a Ptyas mucosus farm in Hainan, China.

Aim: To describe the histopathology and etiology of an outbreak of respiratory disease at a Ptyas mucosus farm in Hainan, China. Methods and results: The etiology was confirmed by gross examination and microscopic analysis. The bacterial isolates from blood and internal organs were identified by biochemical analysis and 16S rRNA gene sequencing. The virulence and antibiotic resistance characteristics of the isolates were further demonstrated by polymerase chain reaction (PCR), disk diffusion testing, and LD50 analysis in Kunming mice. Histopathological analysis of the diseased P. mucosus revealed systemic lesions, including severe airway obstruction with large numbers of inflammatory cells and cellulose exudates in the lungs; severe multifocal hepatocyte vacuolar degeneration and necrosis in the liver with excessive inflammatory exudates and chronic granuloma; splenic hemorrhage and partial loss of splenic structure; and renal vascular and interstitial congestion. Providencia rettgeri was isolated from the blood and multiple internal organs (liver, spleen, kidneys, and lungs). All examined isolates (H1, H4, and H13) were multidrug-resistant but sensitive to four antibiotics-cefepime, imipenem, chloramphenicol, and ciprofloxacin. Both H1 and H4 carried five resistance genes [bla OXA, tet(A), tet(B), tet(E), and aac (3)-IIa], whereas H13 only carried the tet(A) gene. The dominant virulence pattern of the three isolates was hlyA + ZapA + luxS + rsbA. The virulence of H1 strain was tested, and its 50% lethal dose (LD50) in mice was 2.29 × 108 CFU ml-1. Conclusion: To our knowledge, this is the first study to describe an outbreak of bacteremia caused by P. rettgeri in farmed rat snakes. Significance and impact of the study: The results highlight that P. rettgeri is an emerging bacterial pathogen in farmed reptiles.

Photo-catalytic and biological applications of phyto-functionalized zinc oxide nanoparticles synthesized using a polar extract of Equisetum diffusum D.

In this study, zinc oxide nanoparticles (ZnO NPs) were fabricated using Equisetum diffusum D extract and their diverse properties and applications were studied. Phytochemical analysis confirmed the presence of phenols and flavonoids in the plant extract, playing a crucial role in the stabilization and reduction of the synthesized nanoparticles. The greenly synthesized ZnO NPs were characterized through a range of analytical techniques. UV-visible spectrophotometry has been employed to investigate their optical characteristics. FTIR spectroscopy was employed to identify the functional groups responsible for the synthesis of the ZnO NPs. The structural properties were evaluated using XRD. The morphology and size distribution of the synthesized NPs were examined using SEM, DLS, and elemental spectra evaluated using EDX. The charge that develops at the interface was analyzed using zeta potential which accounts for stability of the NPs. The ZnO NPs exhibited excellent photocatalytic degradation of cationic (methylene blue), anionic (methyl orange), and nonionic (p-nitrophenol) dyes under sunlight exposure with photocatalytic degradation of 85.61%, 79.10%, and 89.95% respectively. Additionally, the nanoparticles displayed antimicrobial activity against Gram-positive and Gram-negative bacteria, and noteworthy antioxidant potential. The anti-inflammatory activity of the ZnO NPs, attributed to their ability to inhibit protein denaturation, was dose-dependent. Overall, our findings highlight the versatile properties of the greenly synthesized ZnO NPs, showcasing their potential in environmental remediation, and antimicrobial formulations, and as promising candidates for further exploration in the biomedical fields, including drug delivery and therapeutics.

Comparative study on efficiency of surface enhanced coal fly ash and raw coal fly ash for the removal of hazardous dyes in wastewater: optimization through response surface methodology.

Crystal violet (CV) dye, because of its non-biodegradability and harmful effects, poses a significant challenge for wastewater treatment. This study addresses the efficiency of easily accessible coal fly ash (CFA)-based adsorbents such as raw coal fly ash (RCFA) and surface enhanced coal fly ash (SECFA), in removing CV dye from waste effluents. Various analytical techniques such as FTIR, XRD, SEM, TEM, BET, zeta sizer and zeta potential were employed for the characterization of the adsorbents and dye-loaded samples. BET revealed that RCFA possesses a surface area of 19.370 m2 g-1 and SECFA of 27.391 m2 g-1, exhibiting pore volumes of 0.1365 cm3 g-1 and 0.1919 cm3 g-1 respectively. Zeta-sizer and potential analysis showed the static charges of RCFA as -27.3 mV and SECFA as -28.2 mV, with average particle sizes of 346.6 and 315.3 nm, respectively. Langmuir and Freundlich adsorption isotherms were also employed for adsorption studies. Employing central composite design (CCD) of response surface methodology (RSM), the maximum CV removal was 81.52% for RCFA and 97.52% for SECFA, providing one minute contact time, 0.0125 g adsorbent dose and 10 ppm dye concentration. From the thermodynamic studies, all the negative values of ΔG° showed that all the adsorption processes of both adsorbents were spontaneous in nature.

Evaluation of the anti-inflammatory, antinociceptive, and antipyretic potential of ethanolic extract of Aristida depressa Retz through in vitro, in vivo, and in silico models.

Uncontrolled inflammation is a crucial factor in the development of many diseases. Anti-inflammatory molecules based on natural sources are being actively studied, among which Aristida depressa Retz (Ar.dp) has been traditionally used as a paste to heal inflammation. The present study aimed to evaluate the anti-inflammatory, analgesic, and antipyretic potential of an ethanolic extract of A. depressa through a battery of in vivo and in vitro models. The ethanolic extract of A. depressa was prepared by maceration and chemically characterized using high-performance liquid chromatography, which revealed the presence of quercetin, vanillic acid, chlorogenic acid, p-coumaric acid, m-coumaric acid, ferulic acid, cinnamic acid, and sinapic acid; its antioxidant capacity was then screened with the DPPH in vitro assay, which indicated moderate scavenging capacity. A protein denaturation assay was next performed to evaluate the in vitro anti-inflammatory potential of Ar.dp, which showed significant inhibition (44.44%) compared to the standard drug (diclofenac sodium), with 89.19% inhibition at a concentration of 1 mg/mL. The in vivo safety profile of Ar.dp was evaluated in accordance with the OECD-425 acute toxicity guidelines and found to be safe up to 5 g/kg. The in vivo anti-inflammatory potentials of Ar.dp were evaluated at three different doses (125, 250, and 500 mg/kg) in acute (carrageenan-induced edema: 84.60%, histamine-induced paw edema: 84%), sub-chronic (cotton-pellet-induced granuloma: 57.54%), and chronic (complete-Freund's-adjuvant-induced arthritis: 82.2%) models. Our results showed that Ar.dp had significant (p < 0.05) anti-inflammatory effects over diclofenac sodium in the acute and chronic models. Histopathology studies indicated reduced infiltration of paw tissues with inflammatory cells in Ar.dp-treated animals. Similarly, Ar.dp showed significant (p < 0.05) analgesic (yeast-induced-pyrexia model: 23.53%) and antipyretic (acetic-acid-induced writhing model: 51%) effects in a time-dependent manner. In silico studies on the interactions of COX-1 and COX-2 with the eight ligands mentioned earlier confirmed the inhibition of enzymes responsible for inflammation and fever. Based on the findings of the present study, it is concluded that Ar.dp has anti-inflammatory, analgesic, and antipyretic properties that are likely linked to its pharmacologically active phenolic bioactive molecules.