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Blefaroptosis/etiología , Mieloma Múltiple/complicaciones , Anciano , Blefaroptosis/diagnóstico por imagen , Blefaroptosis/terapia , Femenino , Humanos , Imagen por Resonancia Magnética , Mieloma Múltiple/diagnóstico , Mieloma Múltiple/diagnóstico por imagen , Tomografía de Emisión de PositronesAsunto(s)
Encefalitis Antirreceptor N-Metil-D-Aspartato/diagnóstico por imagen , Tomografía Computarizada por Tomografía de Emisión de Positrones , Adulto , Encefalitis Antirreceptor N-Metil-D-Aspartato/líquido cefalorraquídeo , Encefalitis Antirreceptor N-Metil-D-Aspartato/etiología , Autoanticuerpos/líquido cefalorraquídeo , Autoanticuerpos/inmunología , Autoantígenos/inmunología , Diagnóstico Diferencial , Femenino , Radioisótopos de Flúor , Fluorodesoxiglucosa F18 , Humanos , Encefalitis Límbica/diagnóstico por imagen , Imagen por Resonancia Magnética , Neoplasias Primarias Desconocidas/diagnóstico por imagen , Radiofármacos , Receptores de N-Metil-D-Aspartato/inmunologíaRESUMEN
Wide-neck intracranial aneurysms remain a challenge to endovascular treatment. We describe our experience in repairing wide-neck aneurysms of the anterior circulation located at arterial branch points using coil embolization assisted by Y-stenting using two Solitaire(®) stents.Six wide-neck intracranial aneurysms located on the middle cerebral artery bifurcation( 3), pericallosal artery( 1), and anterior communicating artery( 2) were repaired by Y-stent-assisted coil embolization using two Solitaire(®) stents. Four cases were incidental findings of aneurysm and two cases were previously treated ruptured aneurysms that had undergone recanalization. All the cases were successfully treated without complications. Follow-up by digital subtraction angiography and magnetic resonance angiography at six months showed the stents to be patent with no recanalization of the aneurysm sacs. Repairing wide-neck aneurysms of the anterior circulation by Y-stent-assisted coil embolization using two Solitaire(®) stents is a simple and safe method of treating complex aneurysms. While the results are promising, larger series with longer term follow-ups are needed to corroborate that this treatment method is superior to other techniques.
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Embolización Terapéutica/instrumentación , Aneurisma Intracraneal/terapia , Stents , Adulto , Anciano , Anciano de 80 o más Años , Angiografía de Substracción Digital , Femenino , Humanos , Imagenología Tridimensional , Angiografía por Resonancia Magnética , Masculino , Persona de Mediana Edad , Radiografía Intervencional , Resultado del TratamientoRESUMEN
INTRODUCTION: Training and information for university nursing students about the organ donation and transplantation process is necessary because it will influence their attitudes toward the subject. We analyzed attitudes toward organ donation among nursing students in a donation and transplantation training course and any changes in opinions as a result of the course. MATERIALS AND METHODS: We questioned 48 students in the third year of nursing (University of Murcia, Spain) who were attending a 32-hour training course about donation and transplantation. We used a descriptive concurrent study, through the completion of a validated opinion survey with 27 items before and after the training course. RESULTS: Attitudes toward donation were favorable in 87% of respondents increasing to 94% after course completion. Before starting the course, 87% believed that there were not enough transplantable organs available to cover needs compared to 96% after the course. Before the course, 46% stated that they did not have complete information about the subject. Taking part in the course has encouraged family discussion about the subject (85% to 90%) and improved knowledge about family opinions (64% to 83%; P = .031). Attitudes toward living donation did not change after the course. However, there was an improvement in knowledge of the Spanish organ distribution system. CONCLUSIONS: Attitudes toward organ donation among third-year nursing students were favorable, and increased after undergoing a course about donation and transplantation. The most important part of the course was the increase in theoretical knowledge about the matter as well as the health education.
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Concienciación , Educación en Enfermería , Estudiantes de Enfermería , Trasplante/psicología , Actitud Frente a la Muerte , Actitud Frente a la Salud , Cadáver , Curriculum , Política de Salud , Promoción de la Salud , Humanos , Política Pública , España , Donantes de Tejidos , Obtención de Tejidos y ÓrganosRESUMEN
We describe the case of a 64-year-old woman with a previous diagnosis of celiac sprue and no clinical or histological response to gluten withdrawal. The patient presented a history of longstanding recurrent watery diarrhea and was found to have collagenous colitis after further investigation of her diarrhea. Immunological study was incompatible with celiac disease and no other cause of villous atrophy was found. We suggest that this patient may have a separate disease entity unrelated to celiac sprue and consisting of a pan-intestinal inflammatory disorder characterized by the combination of a chronic inflammatory infiltrate in the small and lower bowel together with a subepithelial collagenous band in the colon.
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Colágeno/metabolismo , Colon/patología , Duodeno/patología , Enterocolitis/patología , Atrofia , Colon/metabolismo , Colonoscopía , Diarrea/etiología , Diarrea/terapia , Duodenoscopía , Duodeno/metabolismo , Enterocolitis/metabolismo , Enterocolitis/terapia , Femenino , Humanos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patología , Persona de Mediana Edad , Resultado del TratamientoRESUMEN
Monoamine oxidase (MAO) A and B and semicarbazide-sensitive amine oxidase (SSAO) localizations in peripheral human tissues were compared by immunohistochemistry. The primary antibodies used were mouse monoclonal anti-human MAO-A (6G11/E1) and anti-human MAO-B (3F12/G10/2E3) and a rabbit polyclonal anti-bovine SSAO antibody. Immunoreactivities of the samples, obtained from 6 routine autopsy cases, showed different distributions in the tissues studied (heart, lung, duodenum, liver, pancreas, spleen, thyroid gland, adrenal gland and kidney). The relative MAO-A, MAO-B and SSAO distributions indicated a widespread distribution of these enzymes in the human body that is characterized by a matching cellular pattern in only few tissues. These differences suggest that each amine oxidase may play a specific function in, at least some, peripheral tissues.
RESUMEN
We studied the localization of monoamine oxidase (MAO) A and B in human heart, liver, duodenum, blood vessels and kidney by immunohistochemistry. The primary antibodies used were mouse monoclonal anti-human MAO-A (6G11/E1) and anti-human MAO-B (3F12/G10/2E3). Samples were obtained from six routine autopsy cases and fixed in 2% paraformaldehyde. All cardiomyocytes and hepatocytes showed MAO-A and MAO-B immunoreactivity. In the duodenum, both immunoreactivities were present in all cells of the villi, Lieberkühn crypts, muscularis mucosae and muscular layers, whereas Brunner glands were devoid of MAO-A and MAO-B staining. Endothelial cells of lymphatic vessels showed MAO-A but no MAO-B immunoreactivity, whereas arteries and veins presented MAO-A and MAO-B staining in muscular layers and fibroblasts but not in endothelial cells. In the kidney, renal tubuli showed MAO-A and MAO-B immunoreactivities, whereas collecting ducts and the Bowman's capsule showed only MAO-A staining. These data represent the first study of the cellular distribution of MAO-A and MAO-B in these human tissues. They show that both enzymes have a widespread distribution in the human body with a matching pattern in many, but not all tissues, and with strong differences from the pattern of distribution in rodents.
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Monoaminooxidasa/análisis , Anciano , Anciano de 80 o más Años , Especificidad de Anticuerpos , Vasos Sanguíneos/citología , Vasos Sanguíneos/enzimología , Duodeno/citología , Duodeno/enzimología , Femenino , Humanos , Inmunohistoquímica , Riñón/citología , Riñón/enzimología , Hígado/citología , Hígado/enzimología , Masculino , Persona de Mediana Edad , Monoaminooxidasa/inmunología , Miocardio/citología , Miocardio/enzimología , Distribución TisularRESUMEN
In order to better delineate the intracellular signaling pathways underlying glial apolipoprotein E (apoE) expression and release, we have characterized an in vitro model of induction of glial apoE production induced by neuronal death. Exposure of mixed fetal cortical neuron/glia co-cultures to the neurotoxin N-methyl-D-aspartate results in increased apoE expression and release in a time- and concentration-dependent manner. Increased expression of apoE messenger RNA precedes the increase in intracellular apoE, followed by accumulation of the holoprotein in the culture medium. Neuronal injury induced by N-methyl-D-aspartate is accompanied by a reactive astrogliosis as measured by an increase in glial fibrillary acidic protein messenger RNA and protein at 48 and 72h post-lesion, respectively. A similar microgliosis was observed using the microglial marker ED-1. Neuronal injury-induced glial apoE secretion is attenuated by the nuclear factor kappaB inhibitors, aspirin, Bay 11-7082 and MG-132, suggesting that this transcription factor is involved in both constitutive and induced glial apoE expression. The present data show that up-regulation of apoE is an early event in the glial activation triggered by neurodegeneration in vitro and that activation of nuclear factor kappaB directly or indirectly mediates the increase in apoE expression.
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Enfermedad de Alzheimer/metabolismo , Apolipoproteínas E/genética , FN-kappa B/metabolismo , Degeneración Nerviosa/metabolismo , Neuroglía/metabolismo , Neuronas/metabolismo , Enfermedad de Alzheimer/fisiopatología , Animales , Anticuerpos/efectos de los fármacos , Anticuerpos/metabolismo , Apolipoproteínas E/metabolismo , Muerte Celular/efectos de los fármacos , Muerte Celular/fisiología , División Celular/efectos de los fármacos , División Celular/fisiología , Células Cultivadas/efectos de los fármacos , Células Cultivadas/metabolismo , Técnicas de Cocultivo , Relación Dosis-Respuesta a Droga , Embrión de Mamíferos , Agonistas de Aminoácidos Excitadores/farmacología , Proteína Ácida Fibrilar de la Glía/efectos de los fármacos , Proteína Ácida Fibrilar de la Glía/metabolismo , Modelos Animales , N-Metilaspartato/farmacología , FN-kappa B/efectos de los fármacos , FN-kappa B/farmacología , Degeneración Nerviosa/fisiopatología , Neuroglía/efectos de los fármacos , Neuronas/efectos de los fármacos , ARN Mensajero/efectos de los fármacos , ARN Mensajero/metabolismo , Ratas , Ratas Sprague-Dawley , Sinaptofisina/efectos de los fármacos , Sinaptofisina/metabolismo , Factores de Tiempo , Regulación hacia Arriba/efectos de los fármacos , Regulación hacia Arriba/fisiologíaRESUMEN
Age-related changes of MAO-A and -B were studied in human and BL/C57 mouse brain areas (substantia nigra, putamen and cerebellum). [3H]Ro41-1049 and [3H]lazabemide were used as selective radioligands to image and quantify MAO-A and MAO-B respectively by enzyme autoradiography. MAO-A binding was higher in mouse, whereas MAO-B binding was higher in human. With aging, mouse MAO-A was significantly reduced between 4 and 8 weeks and remained unchanged until 19 months followed by a slight increase between 19 and 25 months. In contrast, no clear variation was observed in humans between the age of 17-93 years. In most of the structures studied a clear age-related increase in MAO-B was observed beginning in mouse brain at 4 weeks, whereas in human tissue this increase started at the age of 50-60 years. These results show marked differences in the levels and variations of mouse and human MAO-A and -B associated with aging and should be taken into account when extrapolating experimental data from mouse to human.
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Encéfalo/metabolismo , Monoaminooxidasa/metabolismo , Adolescente , Adulto , Factores de Edad , Anciano , Anciano de 80 o más Años , Animales , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Persona de Mediana Edad , Especificidad de la EspecieRESUMEN
Histological analyses were performed on the brains of APP(V717F) transgenic (Tg)mice previously studied in a battery of behavioral tests. We describe here the regional and age-dependent deposition of amyloid in both heterozygous and homozygous Tg mice. We also report that Tg mice show significant and age-dependent changes in synaptic density measured by synaptophysin immunoreactivity. Surprisingly, a rather marked hippocampal atrophy is observed as early as 3 months of age in Tg mice (20-40%). Statistical analyses revealed that the deficits in object recognition memory are related to the number of amyloid deposits in specific brain regions, whereas deficits in spatial reference and working memory are related to the changes in synaptic density and hippocampal atrophy. Our study suggests that the behavioral deficits observed in Tg mice are only in part related to amyloid deposition, but are also related to neuroanatomical alterations secondary to overexpression of the APP(V717F) transgene and independent of amyloid deposition.
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Enfermedad de Alzheimer/patología , Conducta Animal/fisiología , Encéfalo/patología , Factores de Edad , Enfermedad de Alzheimer/fisiopatología , Péptidos beta-Amiloides/análisis , Animales , Modelos Animales de Enfermedad , Inmunohistoquímica , Trastornos de la Memoria/patología , Trastornos de la Memoria/fisiopatología , Ratones , Ratones Transgénicos , Sinaptofisina/análisisRESUMEN
The transcription factor PU.1 has a pivotal role in both the generation and function of macrophages. To determine whether PU.1 is also involved in microglial regulation, we investigated its expression following hypoxic-ischemia (HI) brain injury and in the BV-2 microglial cell line. We found that microglia constitutively expressed high levels of PU.1 protein in both their 'resting' and 'activated' states.
Asunto(s)
Encéfalo/metabolismo , Hipoxia-Isquemia Encefálica/metabolismo , Microglía/metabolismo , Proteínas Proto-Oncogénicas/biosíntesis , Transactivadores/biosíntesis , Animales , Western Blotting , Línea Celular , Inmunohistoquímica , Ratas , Ratas Wistar , Factores de Tiempo , Regulación hacia ArribaRESUMEN
In human brain, nonartherosclerotic calcification is associated with normal aging and several pathological conditions without any clear significance. In all situations, calcification appears predominantly in the basal ganglia, but is also frequent in the hippocampus and cerebral cortex. alpha-Amino-(3-hydroxi-5-methyl-4-isoxazol-4-il)-propionic acid-induced lesion of the globus pallidus is associated in rats with the formation of calcium deposits similar to those observed in the human brain. To determine whether direct neuronal activation may induce calcification, N-methyl-d-aspartate (NMDA) was microinjected in rat hippocampus, globus pallidus, and lateral prefrontal cortex. Two months later, neuronal death was associated with calcium deposits that were characterized in terms of distribution and size. A unique population of deposits was present in the hippocampus and prefrontal cortex, whereas in the globus pallidus two main groups could be differentiated. Calcification was always associated with a significant microglial reaction as shown by the peripheral benzodiazepine receptor autoradiography. Monoamine oxidase B autoradiography, reflecting the astroglial reaction, was also significantly increased. Our results provide evidence that acute NMDA neuronal activation leads with time to calcification associated with a glial reaction and indicate that nonartherosclerotic calcification in the human brain may develop from an acute NMDA receptor activation. A key role of the metabotropic mGluR1 receptor is also suggested.
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Ganglios Basales/efectos de los fármacos , Agonistas de Aminoácidos Excitadores/toxicidad , Hipocampo/efectos de los fármacos , N-Metilaspartato/toxicidad , Corteza Prefrontal/efectos de los fármacos , Animales , Autorradiografía , Ganglios Basales/patología , Calcinosis/patología , Agonistas de Aminoácidos Excitadores/administración & dosificación , Globo Pálido/efectos de los fármacos , Globo Pálido/patología , Hipocampo/patología , Humanos , Masculino , Microinyecciones , Monoaminooxidasa/análisis , N-Metilaspartato/administración & dosificación , Neuronas/efectos de los fármacos , Neuronas/metabolismo , Neuronas/patología , Especificidad de Órganos , Corteza Prefrontal/patología , Células Piramidales/efectos de los fármacos , Células Piramidales/metabolismo , Células Piramidales/patología , Ratas , Ratas Sprague-Dawley , Receptores de GABA-A/análisis , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico/toxicidadRESUMEN
We studied monoamine oxidase (MAO) A and B localization in human pancreas, thyroid gland, and adrenal gland by immunohistochemistry. The primary antibodies used were mouse monoclonal anti-human MAO-A (6G11/E1) and anti-human MAO-B (3F12/G10/2E3). Samples were obtained from six routine autopsy cases and fixed in 2% paraformaldehyde. Exocrine pancreas showed a widespread distribution of MAO-A, whereas MAO-B was present only in centroacinar cells and epithelial cells of pancreatic ducts. In endocrine pancreas, MAO-A was observed in around 50% of islet cells, whereas MAO-B was less abundant and was restricted to the periphery of islets. Thyroid gland showed strong MAO-A immunoreactivity in all cell types and was MAO-B-negative. In adrenal gland, the capsule displayed MAO-A but not MAO-B immunoreactivity, whereas the cortex showed widespread MAO-A staining but was MAO-B-negative in interstitial cells. Finally, in the medulla only a few scattered cells showed either MAO-A or MAO-B immunoreactivity. To our knowledge, these data represent the first study of the cellular distribution of MAO-A and MAO-B in the three human tissues included.
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Glándulas Suprarrenales/enzimología , Monoaminooxidasa/aislamiento & purificación , Páncreas/enzimología , Glándula Tiroides/enzimología , Anciano , Anciano de 80 o más Años , Especificidad de Anticuerpos , Femenino , Humanos , Inmunohistoquímica , Islotes Pancreáticos/enzimología , Isoenzimas/inmunología , Isoenzimas/aislamiento & purificación , Masculino , Persona de Mediana Edad , Monoaminooxidasa/inmunologíaRESUMEN
Localisation of MAO-A and -B in human lung and spleen was studied by immunohistochemistry. The primary antibodies used were mouse monoclonal anti-human MAO-A (6G11/E1) and anti-human MAO-B (3F12/G10/2E3). Samples of lung and spleen were obtained from 6 routine autopsy cases. Both immunoreactivities showed a homogeneous distribution in lung, where all cell types had both MAO-A and -B staining. In spleen MAO-A and -B showed a very weak immunoreactivity, which was restricted to smooth muscle cells and reticular cells of the white pulp. These data represent the most comprehensive study of MAO-A and -B localisation in the two tissues.
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Pulmón/química , Monoaminooxidasa/análisis , Bazo/química , Anciano , Animales , Células Epiteliales/química , Células Epiteliales/enzimología , Femenino , Humanos , Pulmón/enzimología , Masculino , Ratones , Bazo/enzimologíaRESUMEN
Previous data from our laboratory indicate that 25 mM ibotenic acid induces intracellular calcifications in the rat basal forebrain. Because of the lack of specificity of ibotenic acid for a glutamate receptor subtype, a dose-response study with alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate was undertaken and calcified areas (identified with Alizarin Red staining) as well as astro- and microglial reactions (by autoradiography with [3H]lazabemide and [3H]Ro 5-4864) were quantified at one month post-lesion. alpha-Amino-3-hydroxy-5-methyl-4-isoxazole propionate administered into the globus pallidus induced, in a dose-dependent manner, the formation of calcium deposits and the activation of both glial cells, the microglial reaction being particularly robust. From this study, a dose of 5.4 mM alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate was selected for further experiments. [3H]alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate, [3H]dizocilpine maleate and [3H]PN 200-110 binding in vitro were performed to assess autoradiographically whether the tissue damage was associated with changes in glutamate receptors and calcium channel binding sites. In the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate-treated animals, the specific binding of [3H]alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate was significantly reduced by 28% in the lesioned ventral pallidum, whereas it was unchanged in the globus pallidus and substantia innominata. In these three nuclei, calcifications developed and an increase in both glial markers was measured. In contrast, the binding of [3H]PN 200-110 and [3H]dizocilpine maleate were unaffected. Co-injection of 15 mM 6-nitro-7-sulphamoylbenzo(f)quinoxaline-2,3-dione, a selective alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate/kainate receptor antagonist, prevented the formation of calcium concretions, the microglial reaction and the decrease in [3H]alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate binding but it failed to inhibit totally the astroglial reaction induced by alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate. This may suggest that the microglial reaction and calcification take place through different mechanisms from the astrogliosis associated with the neuronal loss. In conclusion, acute administration of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate in the rat globus pallidus elicits a dose-dependent calcification process associated with a chronic reaction of astrocytes and microglia. alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate-induced injury is accompanied by a slight reduction of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptors in the ventral pallidum, whereas the binding of N-methyl-D-aspartate and L-type calcium channels receptors remains unchanged in any lesioned nucleus.
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Astrocitos/patología , Ganglios Basales/efectos de los fármacos , Agonistas de Aminoácidos Excitadores/farmacología , Antagonistas de Aminoácidos Excitadores/farmacología , Quinoxalinas/farmacología , Receptores AMPA/metabolismo , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiónico/farmacología , Animales , Astrocitos/química , Autorradiografía , Ganglios Basales/enzimología , Ganglios Basales/patología , Química Encefálica/efectos de los fármacos , Calcinosis/inducido químicamente , Calcio/análisis , Calcio/metabolismo , Bloqueadores de los Canales de Calcio/metabolismo , Bloqueadores de los Canales de Calcio/farmacología , Canales de Calcio Tipo L/análisis , Canales de Calcio Tipo L/metabolismo , Relación Dosis-Respuesta a Droga , Proteína Ácida Fibrilar de la Glía/análisis , Isradipino/metabolismo , Isradipino/farmacología , Masculino , Microglía/química , Microglía/patología , Monoaminooxidasa/análisis , Inhibidores de la Monoaminooxidasa/metabolismo , Inhibidores de la Monoaminooxidasa/farmacología , Nimodipina/metabolismo , Nimodipina/farmacología , Ácidos Picolínicos/metabolismo , Ácidos Picolínicos/farmacología , Ensayo de Unión Radioligante , Ratas , Ratas Sprague-Dawley , Receptores AMPA/análisis , Receptores de GABA-A/metabolismo , Receptores de N-Metil-D-Aspartato/análisis , Receptores de N-Metil-D-Aspartato/metabolismo , TritioRESUMEN
We quantified the amount of amyloid beta-peptide (Abeta) immunoreactivity as well as amyloid deposits in a large cohort of transgenic mice overexpressing the V717F human amyloid precursor protein (APP(V717F+/-) TG mice) with no, one, or two mouse apolipoprotein E (Apoe) alleles at various ages. Remarkably, no amyloid deposits were found in any brain region of APP(V717F+/-) Apoe(-/-) TG mice as old as 22 mo of age, whereas age-matched APP(V717F +/-) Apoe(+/-) and Apoe(+/+) TG mice display abundant amyloid deposition. The amount of Abeta immunoreactivity in the hippocampus was also markedly reduced in an Apoe gene dose-dependent manner (Apoe(+/+) > Apoe(+/-) >> Apoe(-/-)), and no Abeta immunoreactivity was detected in the cerebral cortex of APP(V717F+/-) Apoe(-/-) TG mice at any of the time points examined. The absence of apolipoprotein E protein (apoE) dramatically reduced the amount of both Abeta(1-40) and Abeta(1-42) immunoreactive deposits as well as the resulting astrogliosis and microgliosis normally observed in APP(V717F) TG mice. ApoE immunoreactivity was detected in a subset of Abeta immunoreactive deposits and in virtually all thioflavine-S-fluorescent amyloid deposits. Because the absence of apoE alters neither the transcription or translation of the APP(V717F) transgene nor its processing to Abeta peptide(s), we postulate that apoE promotes both the deposition and fibrillization of Abeta, ultimately affecting clearance of protease-resistant Abeta/apoE aggregates. ApoE appears to play an essential role in amyloid deposition in brain, one of the neuropathological hallmarks of Alzheimer's disease.
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Enfermedad de Alzheimer/patología , Precursor de Proteína beta-Amiloide/genética , Amiloide/metabolismo , Apolipoproteínas E/genética , Encéfalo/patología , Enfermedad de Alzheimer/genética , Amiloide/aislamiento & purificación , Precursor de Proteína beta-Amiloide/metabolismo , Animales , Apolipoproteínas E/aislamiento & purificación , Apolipoproteínas E/metabolismo , Corteza Cerebral/patología , Modelos Animales de Enfermedad , Gliosis , Heterocigoto , Hipocampo/patología , Homocigoto , Ratones , Ratones Noqueados , Ratones Transgénicos , Neuroglía/metabolismoRESUMEN
Insulin-like growth factor 1 (IGF-1) plays a critical role in CNS development. IGF-1 can block neuronal apoptosis in vitro and in vivo. IGF-1 is thought to be cleaved into des-N-(1-3)-IGF-1 and an amino terminal glycine-proline-glutamate (GPE tripeptide). Here we report a neuroprotective role for GPE tripeptide, with enhanced survival of the CA1-2 hippocampal neurons following an excitotoxic insult in vitro. Binding and displacement studies suggest uniquely distributed sites of action within the rat including the hippocampal CA1-2, pyriform cortex, amygdala, choroid plexus, blood vessels and to a lesser extent in the cortical regions. A similar pattern of binding was seen in the human. This finding could lead to new strategies to reduce neuronal death after injury and in disease.
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Hipocampo/efectos de los fármacos , Factor I del Crecimiento Similar a la Insulina/química , Fármacos Neuroprotectores/farmacología , Oligopéptidos/farmacología , Animales , Autorradiografía , Sitios de Unión , Supervivencia Celular/efectos de los fármacos , Hipocampo/citología , Hipocampo/metabolismo , Humanos , Neuronas/efectos de los fármacos , Fármacos Neuroprotectores/metabolismo , Oligopéptidos/metabolismo , Técnicas de Cultivo de Órganos , RatasRESUMEN
BACKGROUND: Herpes simplex virus (HSV) can produce persistent mucocutaneous disease in patients with the acquired immunodeficiency syndrome (AIDS). In this case report, we evaluate the efficacy, safety and viral resistance after topical foscarnet in severe genital ulceration due to acyclovir-resistant HSV-2. CASE REPORT: A 45-year-old African woman was known for an HIV infection with severe immunosuppression (CD4 <100/mm3). She had received a long-term prophylaxis with acyclovir (400 mg b.i.d.) for a recurrent genital herpes. Few weeks after stopping this prophylaxis, she developed large genital ulcerations progressing despite valacyclovir treatment (1,000 mg t.i.d.). Cultures were positive for HSV-2, resistance to acyclovir was shown by the plaque reduction assay and topical foscarnet was tried. Treatment consisted of a 20-min application of topical foscarnet 2.4% twice a day. Dramatic improvement was observed with rapid antalgia, and cicatrization of the genital ulcerations was observed after 50 days. HSV could not be detected on the mucosal surface. Initially, HSV-2 was resistant to acyclovir but sensitive to foscarnet. After 1 month of topical treatment, HSV-2 became sensitive to acyclovir and was still sensitive to foscarnet. Finally, after 6 weeks of treatment, no virus could be detected by culture. CONCLUSION: Topical foscarnet (2.4%) is a convenient treatment for chronic genital herpes. Resistance to acyclovir disappears few weeks after stopping this drug and sensitivity to foscarnet persists during the 50 days of treatment.
Asunto(s)
Antivirales/uso terapéutico , Foscarnet/uso terapéutico , Herpes Genital/tratamiento farmacológico , Herpes Simple/tratamiento farmacológico , Úlcera Cutánea/tratamiento farmacológico , Aciclovir/farmacología , Administración Tópica , Antivirales/administración & dosificación , Antivirales/farmacología , Farmacorresistencia Microbiana , Femenino , Foscarnet/administración & dosificación , Enfermedades de los Genitales Femeninos/tratamiento farmacológico , Enfermedades de los Genitales Femeninos/patología , Enfermedades de los Genitales Femeninos/virología , Herpes Genital/patología , Herpes Genital/virología , Herpesvirus Humano 2/efectos de los fármacos , Herpesvirus Humano 2/aislamiento & purificación , Humanos , Pruebas de Sensibilidad Microbiana , Persona de Mediana Edad , Úlcera Cutánea/patología , Úlcera Cutánea/virologíaRESUMEN
Microglial cells play important roles in brain injury and repair and are implicated in diseases such as Alzheimer's disease, Creutzfeldt-Jacob disease, multiple sclerosis, the Aids Dementia Complex and stroke. Despite their importance in neuropathology, the underlying molecular basis for the activation of microglia after brain injury is not understood. We show, using RT-PCR, in situ hybridisation, immunocytochemistry, and electrophoretic mobility shift assay, that the CCAAT-enhancer binding protein alpha (C/EBP alpha), a sequence specific DNA-binding protein, is induced in microglial cells, but not astrocytes or neurons, after hypoxic-ischemic brain injury. These results suggest that C/EBP alpha might regulate gene expression and consequentially have a role in the activation and/or proliferation of microglia following brain injury.