RESUMEN
Aging of hematopoietic stem cells (HSCs) is accompanied by impaired self-renewal ability, myeloid skewing, immunodeficiencies and increased susceptibility to malignancies. Although previous studies highlighted the pivotal roles of individual metabolites in hematopoiesis, comprehensive and high-resolution metabolomic profiles of different hematopoietic cells across ages are still lacking. In this study, we created a metabolome atlas of different blood cells across ages in mice. We reveal here that purine, pyrimidine and retinol metabolism are enriched in young hematopoietic stem and progenitor cells (HSPCs), whereas glutamate and sphingolipid metabolism are concentrated in aged HSPCs. Through metabolic screening, we identified uridine as a potential regulator to rejuvenate aged HSPCs. Mechanistically, uridine treatment upregulates the FoxO signaling pathway and enhances self-renewal while suppressing inflammation in aged HSCs. Finally, we constructed an open-source platform for public easy access and metabolomic analysis in blood cells. Collectively, we provide a resource for metabolic studies in hematopoiesis that can contribute to future anti-aging metabolite screening.
RESUMEN
The completely autotrophic nitrogen removal over nitrite (CANON) process is significantly hindered by prolonged start-up periods and unstable nitrogen removal efficiency. In this study, a novel umbrella basalt fiber (BF) carrier with good biological affinity and adsorption performance was used to initiate the CANON process. The CANON process was initiated on day 64 in a sequencing batch reactor equipped with umbrella BF carriers. During this period, the influent NH4+-N concentration gradually increased from 100 to 200 mg·L-1, and the dissolved oxygen was controlled below 0.8 mg L-1. Consequently, an average ammonia nitrogen removal efficiency (ARE) and total nitrogen removal efficiency (TNRE) of â¼90 and 80% were achieved, respectively. After 130 days, ARE and TNRE remained stable at 92 and 81.1%, respectively. This indicates a reliable method for achieving rapid start-up and stable operation of the CANON process. Moreover, Candidatus Kuenenia and Candidatus Brocadia were identified as dominant anammox genera on the carrier. Nitrosomonas was the predominant genus among ammonia-oxidizing bacteria. Spatial differences were observed in the microbial population of umbrella BF carriers. This arrangement facilitated autotrophic nitrogen removal in a single reactor. This study indicates that the novel umbrella BF carrier is a highly suitable biocarrier for the CANON process.
Asunto(s)
Procesos Autotróficos , Reactores Biológicos , Nitritos , Nitrógeno , Nitrógeno/química , Nitritos/química , Eliminación de Residuos Líquidos/métodos , Bacterias/metabolismoRESUMEN
Background: Cancer-targeted T-cell receptor T (TCR-T) cells hold promise in treating cancers such as hematological malignancies and breast cancers. However, approaches to obtain cancer-reactive TCR-T cells have been unsuccessful. Methods: Here, we developed a novel strategy to screen for cancer-targeted TCR-T cells using a special humanized mouse model with person-specific immune fingerprints. Rare steady-state circulating hematopoietic stem and progenitor cells were expanded via three-dimensional culture of steady-state peripheral blood mononuclear cells, and then the expanded cells were applied to establish humanized mice. The human immune system was evaluated according to the kinetics of dendritic cells, monocytes, T-cell subsets, and cytokines. To fully stimulate the immune response and to obtain B-cell precursor NAML-6- and triple-negative breast cancer MDA-MB-231-targeted TCR-T cells, we used the inactivated cells above to treat humanized mice twice a day every 7 days. Then, human T cells were processed for TCR ß-chain (TRB) sequencing analysis. After the repertoires had been constructed, features such as the fraction, diversity, and immune signature were investigated. Results: The results demonstrated an increase in diversity and clonality of T cells after treatment. The preferential usage and features of TRBV, TRBJ, and the V-J combination were also changed. The stress also induced highly clonal expansion. Tumor burden and survival analysis demonstrated that stress induction could significantly inhibit the growth of subsequently transfused live tumor cells and prolong the survival of the humanized mice. Conclusions: We constructed a personalized humanized mouse model to screen cancer-targeted TCR-T pools. Our platform provides an effective source of cancer-targeted TCR-T cells and allows for the design of patient-specific engineered T cells. It therefore has the potential to greatly benefit cancer treatment.
RESUMEN
Short-term exposure to ozone has been linked to multiple allergic diseases, but the relationship between ozone exposure and allergic conjunctivitis (AC) remains unclear. This study aimed to investigate the association between short-term exposure to ozone and the risk of AC. We conducted a time-stratified case-crossover study across five Chinese cities from 2014 to 2022. Daily outpatient visit records for AC were identified in five hospitals using either the diagnosis name or ICD-10 code H10.1. Data on air pollution and meteorological conditions were also collected. We first examined the city-specific association between short-term ozone exposure and AC using conditional logistic regression. A random-effects meta-analysis was then conducted to obtain overall estimates. During the study period, 130,093 outpatient visits for AC occurred, with 58.8% (76,482) being male and 41.2% (53,611) female. A one-standard-deviation (SD) increase in ozone was associated with an 8.3% increase (95% CI: 3.8%, 13.0%) in AC outpatient visits. Similar positive associations were observed when adjusting for other pollutants (PM2.5, CO, SO2 and NO2) in two-pollutant and multi-pollutant models. Furthermore, the positive association remained consistent when using mixed-effects regression models or further adjusting for meteorological conditions. In addition, no effect modification of the AC-ozone association by sex, age and season was apparent. This study provides evidence supporting a positive association between short-term ozone exposure and AC risk in China. This highlights the potential value of mitigating ozone pollution to reduce the risk of ocular surface disorders.
Asunto(s)
Contaminantes Atmosféricos , Contaminación del Aire , Ciudades , Conjuntivitis Alérgica , Estudios Cruzados , Exposición a Riesgos Ambientales , Ozono , Ozono/análisis , China/epidemiología , Humanos , Contaminantes Atmosféricos/análisis , Masculino , Femenino , Contaminación del Aire/estadística & datos numéricos , Conjuntivitis Alérgica/epidemiología , Adulto , Exposición a Riesgos Ambientales/estadística & datos numéricos , Persona de Mediana Edad , Adolescente , Adulto Joven , Niño , Preescolar , AncianoRESUMEN
Background: Vertical stiffness (Kvert) can be used to evaluate sports performance and injury risk in players. The My Jump 2 smartphone application (App), is increasingly being used by researchers, coaches, and players in the competitive sports field. We aimed to analyze the reliability and concurrent validity of the My Jump 2 app for measuring Kvert in male college players. Methods: Twenty male college players (10 soccer players, 10 basketball players; age, 20.2 ± 1.3 years old; weight, 76.4 ± 6.0â kg; height, 178.3 ± 4.7â cm) volunteered to take part in this study. Three drop jumps were performed by participants from 30â cm to 40â cm on a force platform and retested after three days. All the jumps were recorded by both the Force platform and the My Jump 2 app. Data obtained from the above two devices were compared using the paired t tests, intraclass correlation coefficient (ICC), coefficient of variation (CV), Pearson product moment correlation coefficient (r), Bland-Altman plots, and one-way regression. Results: There was almost perfect agreement between measurement instruments for the Kvert value (ICC > 0.972, 95% CI = 0.954-0.992, P < 0.01). Almost perfect agreement was observed between evaluators (ICC > 0.989, 95% CI = 0.981-0.997, P < 0.05). Also, the My Jump 2 app showed excellent intra-rater reliability in all participants (ICC = 1.000, 95% CI = 1.000-1.000, P < 0.001). The My Jump 2 showed good variability when measuring Kvert at T1 30â cm (CV = 5.4%), T1 40â cm (CV = 6.7%), T2 30â cm (CV = 5.0%), and T2 40â cm (CV = 10.3%). The test-retest reliability of My Jump 2 was moderate to good at 30â cm (ICC = 0.708, 95% CI = 0.509-0.827); however, it was lower to moderate at 40â cm (ICC = 0.445, 95% CI = 0.222-0.625). Very large correlations were observed between the force platform and the My Jump 2 for Kvert (r > 0.9655, P < 0.001). Conclusion: The My Jump 2 smartphone application showed excellent reliability and intra-rater consistency in measuring Kvert in male college players. While demonstrating excellent intra-rater consistency and strong agreement with force platform measurements, it showed slightly lower reliability at higher jump heights. Overall, the My Jump 2 app is a valid tool for evaluating Kvert in college players with careful consideration of its limitations, particularly at higher jump heights.
RESUMEN
Transcriptional regulation mechanisms underlying chilling injury (CI) development have been widely investigated in model plants and cold-sensitive fruits, such as banana (Musa acuminata). However, unlike the well-known NAC and WRKY transcription factors (TFs), the function and deciphering mechanism of heat shock factors (HSFs) involving in cold response are still fragmented. Here, we showed that hot water treatment (HWT) alleviated CI in harvested banana fruits accomplishing with reduced reactive oxygen species (ROS) accumulation and increased antioxidant enzyme activities. A cold-inducible but HWT-inhibited HSF, MaHsf24, was identified. Using DNA affinity purification sequencing (DAP-seq) combined with RNA-seq analyses, we found three heat shock protein (HSP) genes (MaHSP23.6, MaHSP70-1.1 and MaHSP70-1.2) and three antioxidant enzyme genes (MaAPX1, MaMDAR4 and MaGSTZ1) were the potential targets of MaHsf24. Subsequent electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) and dual-luciferase reporter (DLR) analyses demonstrated that MaHsf24 repressed the transcription of these six targets via directly binding to their promoters. Moreover, stably overexpressing MaHsf24 in tomatoes increased cold sensitivity by suppressing the expressions of HSPs and antioxidant enzyme genes, while HWT could recover cold tolerance, maintaining higher levels of HSPs and antioxidant enzyme genes, and activities of antioxidant enzymes. In contrast, transiently silencing MaHsf24 by virus-induced gene silencing (VIGS) in banana peels conferred cold resistance with the upregulation of MaHSPs and antioxidant enzyme genes. Collectively, our findings support the negative role of MaHsf24 in cold tolerance, and unravel a novel regulatory network controlling bananas CI occurrence, concerning MaHsf24-exerted inhibition of MaHSPs and antioxidant enzyme genes.
RESUMEN
BACKGROUND: Growing evidence indicated that to develop of atherosclerosis observed more often by people with Alzheimer's disease (AD), but the underlying mechanism is not fully clarified. Considering that amyloid-ß (Aß) deposition in the brain is the key pathophysiology of AD and plasma Aß is closely relate to Aß deposition in the brain, in the present study, we investigated the relationships between atherosclerosis and plasma Aß levels. METHODS: This was a population based cross-sectional study. Patients with high risk of atherosclerosis from Qubao Village, Xi'an were underwent carotid ultrasound for assessment of atherosclerosis. Venous blood was collected on empty stomach in the morning and plasma Aß1-40 and Aß1-42 levels were measured using ELISA. Multivariate logistic regression analysis was performed to investigate the relationships between carotid atherosclerosis (CAS) and plasma Aß levels. RESULTS: Among 344 patients with high risk of atherosclerosis, 251(73.0%) had CAS. In the univariate analysis, the plasma Aß levels had no significant differences between CAS group and non-CAS group (Aß1-40: 53.07 ± 9.24 pg/ml vs. 51.67 ± 9.11pg/ml, p = 0.211; Aß1-42: 40.10 ± 5.57 pg/ml vs. 40.70 pg/ml ± 6.37pg/ml, p = 0.285). Multivariate logistic analysis showed that plasma Aß levels were not associated with CAS (Aß1-40: OR = 1.019, 95%CI: 0.985-1.054, p = 0.270;Aß1-42: OR = 1.028, 95%CI: 0.980-1.079, p = 0.256) in the total study population. After stratified by hypertension, CAS was associated with plasma Aß1-40 positively (OR = 1.063, 95%CI: 1.007-1.122, p = 0.028) in the non-hypertension group, but not in hypertensive group. When the plasma Aß concentrations were classified into four groups according to its quartile, the highest level of plasma Aß1-40 group was associated with CAS significantly (OR = 4.465, 95%CI: 1.024-19.474, p = 0.046). CONCLUSION: Among patients with high risk of atherosclerosis, CAS was associated with higher plasma Aß1-40 level in non-hypertension group, but not in hypertension group. These indicated that atherosclerosis is associated with plasma Aß level, but the relationship may be confounded by hypertension.
Asunto(s)
Péptidos beta-Amiloides , Aterosclerosis , Fragmentos de Péptidos , Humanos , Masculino , Femenino , Péptidos beta-Amiloides/sangre , Estudios Transversales , Anciano , Persona de Mediana Edad , Aterosclerosis/sangre , Aterosclerosis/epidemiología , Fragmentos de Péptidos/sangre , Factores de Riesgo , Hipertensión/sangre , Hipertensión/epidemiologíaRESUMEN
As a UNESCO World Cultural Heritage, the aesthetic value of bronze artifacts from the Shang and Chow Dynasties has had a profound influence on Chinese traditional culture and art. To facilitate the digital preservation and protection of these Shang and Chow bronze artifacts (SCB), it becomes imperative to categorize their decorative patterns. Therefore, a SCB pattern classification method of differential evolution called Shang and Chow Bronze Convolutional Neural Network (SCB-CNN) is proposed. Firstly, the original bronze decorative patterns of Shang and Chow dynasties are collected, and the samples are expanded through image augmentation technology to form a training dataset. Secondly, based on the classical convolutional neural network structure, the recognition and classification of bronze patterns are implemented by adjusting the network parameters. Then, the initial parameters of the convolutional neural network are optimized by differential evolution algorithm, and the optimized SCB-CNN is simulated. Finally, comparative experiments were conducted between the optimized SCB-CNN, the unoptimized model, VGG-Net, and GoogleNet. The experimental results indicate that the optimized SCB-CNN significantly reduces training time while maintaining fast prediction speed, convergence speed, and high accuracy. This study provides new insights for the inheritance and innovation research of SCB patterns.
Asunto(s)
Algoritmos , Redes Neurales de la Computación , Humanos , China , Arqueología/métodos , Historia AntiguaRESUMEN
Bidirectional electron transfer is about that exoelectrogens produce bioelectricity via extracellular electron transfer at anode and drive cytoplasmic biochemical reactions via extracellular electron uptake at cathode. The key factor to determine above bioelectrochemical performances is the electron transfer efficiency under biocompatible abiotic/biotic interface. Here, a graphene/polyaniline (GO/PANI) nanocomposite electrode specially interfacing exoelectrogens (Shewanella loihica) and augmenting bidirectional electron transfer was conducted by in-situ electrochemical modification on carbon paper (CP). Impressively, the GO/PANI@CP electrode tremendously improved the performance of exoelectrogens at anode for wastewater treatment and bioelectricity generation (about 54 folds increase of power density compared to blank CP electrode). The bacteria on electrode surface not only showed fast electron release but also exhibited high electricity density of extracellular electron uptake through the proposed direct electron transfer pathway. Thus, the cathode applications of microbial electrosynthesis and bio-denitrification were developed via GO/PANI@CP electrode, which assisted the close contact between microbial outer-membrane cytochromes and nanocomposite electrode for efficient nitrate removal (0.333 mM/h). Overall, nanocomposite modified electrode with biocompatible interfaces has great potential to enhance bioelectrochemical reactions with exoelectrogens.
Asunto(s)
Fuentes de Energía Bioeléctrica , Electrodos , Grafito , Grafito/química , Transporte de Electrón , Fuentes de Energía Bioeléctrica/microbiología , Compuestos de Anilina/química , Compuestos de Anilina/metabolismo , Materiales Biocompatibles/química , Materiales Biocompatibles/metabolismo , Shewanella/metabolismo , Nanocompuestos/química , Técnicas Electroquímicas/métodosRESUMEN
Texture softening is a physiological indicator of fruit ripening, which eventually contributes to fruit quality and the consumer's acceptance. Despite great progress having been made in identification of the genes related to fruit softening, the upstream transcriptional regulatory pathways of these softening-related genes are not fully elucidated. Here, a novel bHLH gene, designated as MabHLH28, was identified because of its significant upregulation in banana fruit ripening. DAP-Seq analysis revealed that MabHLH28 bound to the core sequence of 'CAYGTG' presented in promoter regions of fruit softening-associated genes, such as the genes related to cell wall modification (MaPG3, MaPE1, MaPL5, MaPL8, MaEXP1, MaEXP2, MaEXPA2, and MaEXPA15) and starch degradation (MaGWD1 and MaLSF2), and these bindings were validated by EMSA and DLR assays. Transient overexpression and knockdown of MabHLH28 in banana fruit resulted in up- and down-regulation of softening-related genes, thereby hastening and postponing fruit ripening. Furthermore, overexpression of MabHLH28 in tomato accelerated the ripening process by elevating the accumulation of softening-associated genes. In addition, MabHLH28 showed interaction withMaWRKY49/111 and itself to form protein complexes, which could combinatorically strengthen the transcription of softening-associated genes. Taken together, our findings suggest that MabHLH28 mediates fruit softening by upregulating the expression of softening-related genes either alone or in combination with MaWRKY49/111.
RESUMEN
Protein S-acylation catalyzed by protein S-acyl transferases (PATs) is a reversible lipid modification regulating protein targeting, stability, and interaction profiles. PATs are encoded by large gene families in plants, and many proteins including receptor-like cytoplasmic kinases (RLCKs) and receptor-like kinases (RLKs) are subject to S-acylation. However, few PATs have been assigned substrates, and few S-acylated proteins have known upstream enzymes. We report that Arabidopsis (Arabidopsis thaliana) class A PATs redundantly mediate pollen tube guidance and participate in the S-acylation of POLLEN RECEPTOR KINASE1 (PRK1) and LOST IN POLLEN TUBE GUIDANCE1 (LIP1), a critical RLK or RLCK for pollen tube guidance, respectively. PAT1, PAT2, PAT3, PAT4, and PAT8, collectively named PENTAPAT for simplicity, are enriched in pollen and show similar subcellular distribution. Functional loss of PENTAPAT reduces seed set due to male gametophytic defects. Specifically, pentapat pollen tubes are compromised in directional growth. We determine that PRK1 and LIP1 interact with PENTAPAT, and their S-acylation is reduced in pentapat pollen. The plasma membrane (PM) association of LIP1 is reduced in pentapat pollen, whereas point mutations reducing PRK1 S-acylation affect its affinity with its interacting proteins. Our results suggest a key role of S-acylation in pollen tube guidance through modulating PM receptor complexes.
RESUMEN
This study investigates the effects of a 12-week brisk walking exercise regimen on motor function improvements in elderly women. Twenty-six elderly women, aged 84.2 ± 3.2 years, participated in a 12-week brisk walking exercise program. Fitness assessments and blood biomarker analyses (including CHO, HDLC, LDLC, TC) were conducted pre- and post-intervention. Additionally, targeted metabolomics was employed to measure short-chain fatty acids, amino acids, and vitamin metabolites. The intervention led to significant enhancements in participants' flexibility (p < 0.05), lower limb muscle strength (p < 0.01), and cardiorespiratory endurance (p < 0.01), while muscle mass showed no significant changes. Fifteen significant differential metabolites were identified (VIP > 1.0, FC > 1.2 or < 0.8, and p < 0.05), with arginine, ornithine, aspartic acid, glutamine, phenylalanine, tyrosine, and pantothenic acid playing key roles across seven metabolic pathways. A 12-week brisk walking exercise program significantly enhanced flexibility, lower limb muscle strength, and cardiorespiratory endurance among elderly women. These improvements did not extend to muscle mass or upper limb muscle strength. The observed enhancement in exercise capacity may be attributed to improved regulation of neurotransmitters.
Asunto(s)
Ejercicio Físico , Caminata , Femenino , Humanos , China , Ejercicio Físico/fisiología , Extremidad Inferior , Fuerza Muscular , Aptitud Física/fisiología , Caminata/fisiología , Anciano de 80 o más AñosRESUMEN
KCNH2 (Potassium Voltage-Gated Channel Subfamily H Member) encodes a voltage-activated potassium channel role as rapidly activating-delayed rectifier potassium channel that plays an essential role in the final repolarization of the ventricular action potential. Mutations in this gene can cause long QT syndrome and short QT syndrome. Transcript variants encoding distinct isoforms were also identified. In this study, we generated induced pluripotent stem cells (iPSC) from a healthy individual by electroporation of peripheral blood mononuclear cells and generated a KCNH2 heterozygous knockout human iPSC line via CRISPR/Cas9 gene editing. The resulting iPSCs had a normal karyotype, were free of genomically integrated epitomal plasmids, expressed pluripotency markers, and maintained trilineage differentiation potential.
Asunto(s)
Canal de Potasio ERG1 , Heterocigoto , Células Madre Pluripotentes Inducidas , Síndrome de QT Prolongado , Células Madre Pluripotentes Inducidas/metabolismo , Humanos , Canal de Potasio ERG1/genética , Canal de Potasio ERG1/metabolismo , Síndrome de QT Prolongado/genética , Síndrome de QT Prolongado/metabolismo , Síndrome de QT Prolongado/patología , Línea Celular , Sistemas CRISPR-Cas , Técnicas de Inactivación de Genes , Diferenciación Celular , Edición Génica , Arritmias CardíacasRESUMEN
Guavas are typical tropical fruit with high nutritional and commercial value. Because of their thin skin and high metabolic rate, guavas are highly susceptible to water loss, physical damage, and spoilage, severely limiting their shelf-life. Guavas can typically only be stored for approximately one week at room temperature, making transportation, storage, and handling difficult, resulting in low profit margins in the industry. This review focuses on the physiological and biochemical changes and their molecular mechanisms which occur in postharvest guavas, and summarizes the various management strategies for extending the shelf-life of these sensitive fruits by means of physical and chemical preservation and their combinations. This review also suggests future directions and reference ideas for the development of safe and efficient shelf-life extension techniques.
RESUMEN
Although the synthesis of mechanically interlocked molecules has been extensively researched, selectively constructing homogeneous linear [4]catenanes remains a formidable challenge. Here, we selectively constructed a homogeneous linear metalla[4]catenane in a one-step process through the coordination-driven self-assembly of a bidentate benzothiadiazole derivative ligand and a binuclear half-sandwich rhodium precursor. The formation of metalla[4]catenanes was facilitated by cooperative interactions between strong sandwich-type π-π stacking and non-classical hydrogen bonds between the components. Moreover, by modulating the aromatic substituents on the binuclear precursor, two homogeneous metalla[2]catenanes were obtained. The molecular structures of these metallacatenanes were unambiguously characterized by single-crystal X-ray diffraction analysis. Additionally, reversible structural transformation between metal-catenanes and the corresponding metallarectangles could be achieved by altering their concentration, as confirmed by mass spectrometry and NMR spectroscopy studies.
RESUMEN
Electrochemical redox flow desalination is an emerging method to obtain freshwater; however, the costly requirement for continuously supplying and regenerating redox species limits their practical applications. Recycling of spent lithium-ion batteries is a growing challenge for their sustainable utilization. Existing battery recycling methods often involve massive secondary pollution. Here, we demonstrate a redox flow system to couple redox flow desalination with lithium recovery from spent lithium-ion batteries. The spontaneous reaction between a battery cathode material (LiFePO4) and ferricyanide enables the continuous regeneration of the redox species required for desalination. Several critical operating parameters are optimized, including current density, the concentrations of redox species, salt concentrations of brine, and the amounts of added LiFePO4. With the addition of 0.5920 g of spent LiFePO4 in five consecutive batches, the system can operate over 24 h, achieving 70.46 % lithium recovery in the form of LiCl aqueous solution at the concentration of 6.716 g·L-1. Simultaneously, the brine (25 mL, 10000 ppm NaCl) was desalinated to freshwater. Detailed cost analysis shows that this redox flow system could generate a revenue of ¥ 13.66 per kg of processed spent lithium-ion batteries with low energy consumption (0.77 MJ kg-1) and few greenhouse gas emissions indicating excellent economic and environmental benefits over existing lithium-ion battery recycling technologies, such as pyrometallurgical and hydrometallurgical methods. This work opens a new approach to holistically addressing water and energy challenges to achieve sustainable development.
Asunto(s)
Suministros de Energía Eléctrica , Litio , Sales (Química) , Reciclaje/métodos , Agua , Iones , Oxidación-ReducciónRESUMEN
Affected by global warming, the permafrost in Northeast China (NEC) has been continuously degrading in recent years. Many researchers have focused on the spatial and temporal distribution characteristics of permafrost in NEC, however, few studies have delved into the field scale. In this study, based on the Optimal Parameters-based Geographical Detector (OPGD) model and Receiver Operating Characteristic (ROC) test, the spatial stratified heterogeneity of permafrost distribution and the indicating performance of environmental variables on permafrost in NEC at the field scale were analyzed. Permafrost spatial distribution data were obtained from the Engineering Geological Investigation Reports (EGIR) of six highways located in NEC and a total of 19 environmental variables related to heat transfer, vegetation, soil, topography, moisture, and ecology were selected. The H-factors (variables with the highest contribution in factor detector results and interaction detector results): slope position (γ), surface frost number (SFN), elevation (DEM), topographic diversity (TD), and annual snow cover days (ASCD) were found to be the major contributors to the distribution of permafrost at the field scale. Among them, γ has the highest contribution and is a special explanatory variable for permafrost. In most cases, interaction can improve the impact of variables, especially the interaction between H-factors. The risk of permafrost decreases with the increase of TD, RN, and SBD, and increases with the increase of SFN. The performance of SFN to indicate permafrost distribution was found to be the best among all variables (AUC = 0.7063). There is spatial heterogeneity in the distribution of permafrost on highways in different spatial locations. This study summarized the numerical and spatial location between permafrost and different environmental variables at the field scale, and many results were found to be informative for environmental studies and engineering construction in NEC.
Asunto(s)
Hielos Perennes , Suelo , Geografía , Análisis Espacial , ChinaRESUMEN
OBJECTIVE: Insomnia is a common sleep disorder, often associated with some mental disorders or mental health concerns, especially when accompanied by depression or anxiety, but very limited research has been reported in China. The purpose of this study was to investigate the prevalence of insomnia and associations with depression, anxiety in Chinese adults. METHODS: We conducted this large-sample cross-sectional study (51774 adults) in Guangdong province from October to December 2022. We used multistage stratified equal-volume random sampling under a complex sampling design to select the sample and standardized structured questionnaires to collect the necessary information. Descriptive analysis and logistic regression model were used for statistical analysis. RESULTS: The weighted prevalence of insomnia was 24.8 %. Insomnia was significantly associated with depression (OR:11.29, 95 %CI: 9.58-13.29), and anxiety (OR:10.98, 95 %CI: 8.78-13.72). Risk factors as being associated with insomnia were female, higher years of education, suffering from chronic diseases, previous drinking and current drinking, while protective factors were living in a rural area, married or cohabited, divorce or separation and being older. Risk factors as being associated with depression in the insomnia group included 10-16 years of education and suffering from chronic diseases, while protective factors were being older, married or cohabited, and normal BMI. Risk factors associated with anxiety in the insomnia group included 7-12 years of education and suffering from chronic diseases, while protective factors were being older, married or cohabited, and having a normal BMI. CONCLUSIONS: Insomnia is associated with the development of depression and anxiety. Women and unhealthy lifestyle were at high risk for insomnia, had chronic diseases is an important factor, and insomnia with depression or anxiety.
Asunto(s)
Trastornos del Inicio y del Mantenimiento del Sueño , Adulto , Humanos , Femenino , Masculino , Trastornos del Inicio y del Mantenimiento del Sueño/epidemiología , Estudios Transversales , Depresión/epidemiología , Depresión/psicología , Prevalencia , Ansiedad/epidemiología , Ansiedad/psicología , Enfermedad Crónica , China/epidemiologíaRESUMEN
Vascular calcification (VC) is a critical complication in chronic kidney disease (CKD), where transcription factors (TFs) and microRNAs (miRs) could potentially play a pivotal role in its pathogenesis and progression. To explore the potential molecular mechanism by which the TF D-box-binding protein (DBP) regulates the miR-195-5p/cyclin D1 (CCND1) axis and its impact on aortic VC in CKD rats, we established a rat model of CKD with VC through a 5/6 nephrectomy procedure. This model was treated with lentivirus overexpressing DBP or CCND1 to analyze their roles in aortic VC. Additionally, an in vitro cell model of VC was induced by high phosphorus. This model underwent transfection with lentivirus overexpressing DBP or miR-195-5p mimic/inhibitor to confirm their regulatory roles in aortic VC in vitro. We assessed the interactions between DBP and miR-195-5p, as well as between miR-195-5p and CCND1. Our results indicated that the expression of DBP and miR-195-5p was reduced, while CCND1 levels were elevated in both the rat and cell models. Overexpression of miR-195-5p inhibited VC in vascular smooth muscle cells (VSMCs). Bioinformatics prediction and dual luciferase assays confirmed that DBP could act as a TF to enhance miR-195-5p expression, with Ccnd1 identified as a downstream target gene of miR-195-5p. Overexpression of DBP inhibited aortic calcification in CKD rats, whereas overexpression of CCND1 produced the opposite effect. In conclusion, the TF DBP can inhibit CCND1 expression through transcriptional activation of miR-195-5p, thereby preventing VC in rats with CKD.
Asunto(s)
Ciclina D1 , MicroARNs , Insuficiencia Renal Crónica , Calcificación Vascular , Animales , MicroARNs/genética , MicroARNs/metabolismo , Calcificación Vascular/metabolismo , Calcificación Vascular/genética , Calcificación Vascular/patología , Insuficiencia Renal Crónica/metabolismo , Insuficiencia Renal Crónica/genética , Insuficiencia Renal Crónica/patología , Ciclina D1/metabolismo , Ciclina D1/genética , Ratas , Masculino , Ratas Sprague-Dawley , Regulación hacia Abajo , Músculo Liso Vascular/metabolismo , Músculo Liso Vascular/patología , Modelos Animales de Enfermedad , Miocitos del Músculo Liso/metabolismo , Miocitos del Músculo Liso/patologíaRESUMEN
Hematopoietic stem and progenitor cells generate all the lineages of blood cells throughout the lifespan of vertebrates. The emergence of hematopoietic stem and progenitor cells is finely tuned by a variety of signaling pathways. Previous studies have revealed the roles of pattern-recognition receptors such as Toll-like receptors and RIG-I-like receptors in hematopoiesis. In this study, we find that Nlrc3, a nucleotide-binding domain leucine-rich repeat containing family gene, is highly expressed in hematopoietic differentiation stages in vivo and vitro and is required in hematopoiesis in zebrafish. Mechanistically, nlrc3 activates the Notch pathway and the downstream gene of Notch hey1. Furthermore, NF-kB signaling acts upstream of nlrc3 to enhance its transcriptional activity. Finally, we find that Nlrc3 signaling is conserved in the regulation of murine embryonic hematopoiesis. Taken together, our findings uncover an indispensable role of Nlrc3 signaling in hematopoietic stem and progenitor cell emergence and provide insights into inflammation-related hematopoietic ontogeny and the in vitro expansion of hematopoietic stem and progenitor cells.