Asunto(s)
Antígenos CD/análisis , Médula Ósea/patología , Células Madre Hematopoyéticas/inmunología , Síndrome de Inmunodeficiencia Adquirida del Simio/inmunología , Virus de la Inmunodeficiencia de los Simios , Animales , Antígenos CD34 , Antígenos de Diferenciación/análisis , Médula Ósea/inmunología , Separación Celular , Ensayo de Unidades Formadoras de Colonias , ADN Viral/análisis , Ensayo de Inmunoadsorción Enzimática , Células Madre Hematopoyéticas/patología , Macaca mulatta , Reacción en Cadena de la Polimerasa , Provirus/genética , Provirus/aislamiento & purificación , Síndrome de Inmunodeficiencia Adquirida del Simio/patología , Virus de la Inmunodeficiencia de los Simios/aislamiento & purificación , Linfocitos T/inmunología , Linfocitos T/patologíaAsunto(s)
Anticuerpos Antihepatitis/análisis , Hepatitis D/diagnóstico , ARN Viral/análisis , Animales , Antígenos Virales/análisis , Modelos Animales de Enfermedad , Hepatitis B/complicaciones , Hepatitis B/etiología , Hepatitis D/complicaciones , Hepatitis D/etiología , Virus de la Hepatitis Delta/inmunología , Virus de la Hepatitis Delta/aislamiento & purificación , Antígenos de Hepatitis delta , Hepatitis Viral Animal/diagnóstico , Hepatitis Viral Animal/etiología , Inmunoensayo/métodos , Pan troglodytesRESUMEN
9-(2-Phosphonylmethoxyethyl)adenine (PMEA) is a potent and selective inhibitor of the in vitro replication of a number of retroviruses, including HIV-1 and HIV-2, simian immunodeficiency virus (SIV), simian AIDS-related virus (SRV), feline immunodeficiency virus (FIV) and Moloney murine sarcoma virus (MSV). PMEA causes a dose-dependent suppression of the induction of anti-SIVmacgp120 antibodies in SIV mac-infected rhesus monkeys. Complete suppression of anti-SIVmacgp120 antibodies was achieved in SIV-infected animals treated with PMEA at 2 x 10 or 2 x 5 mg/kg per day for 29 days. No toxic side-effects were noted during this treatment period. Antibodies against SIVmac gp120 appeared 1-2 weeks after PMEA treatment was stopped, but the antibody titre reached in these animals was significantly lower than in the SIVmac-infected animals who had not been treated with PMEA. Our data strongly suggest that PMEA should be pursued for its potential in the treatment of AIDS and other retrovirus infections.