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1.
Talanta ; 273: 125971, 2024 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-38521020

RESUMEN

T-2 is one of the most potent cytotoxic food-borne mycotoxins. In this work, we have developed and characterized an electrochemical microfluidic immunosensor for T-2 toxin quantification in wheat germ samples. T-2 toxin detection was carried out using a competitive immunoassay method based on monoclonal anti-T-2 antibodies immobilized on the poly(methyl methacrylate) (PMMA) microfluidic central channel. The platinum wire working electrode at the end of the channel was in situ modified by a single-step electrodeposition procedure with reduced graphene oxide (rGO)-nanoporous gold (NPG). T-2 toxin in the sample was allowed to compete with T-2-horseradish peroxidase (HRP) conjugated for the specific recognizing sites of immobilized anti-T-2 monoclonal antibodies. The HRP, in the presence of hydrogen peroxide (H2O2), catalyzes the oxidation of 4-tert-butylcatechol (4-TBC), whose back electrochemical reduction was detected on the nanostructured electrode at -0.15 V. Thus, at low T-2 concentrations in the sample, more enzymatically conjugated T-2 will bind to the capture antibodies, and, therefore, a higher current is expected. The detection limits found for electrochemical immunosensor, and commercial ELISA procedure were 0.10 µg kg-1 and 10 µg kg-1, and the intra- and inter-assay coefficients of variation were below 5.35% and 6.87%, respectively. Finally, our microfluidic immunosensor to T-2 toxin will significantly contribute to faster, direct, and secure in situ analysis in agricultural samples.


Asunto(s)
Técnicas Biosensibles , Grafito , Nanopartículas del Metal , Micotoxinas , Nanoporos , Toxina T-2 , Grafito/química , Inmunoensayo/métodos , Microfluídica , Oro/química , Técnicas Biosensibles/métodos , Peróxido de Hidrógeno/química , Técnicas Electroquímicas/métodos , Límite de Detección , Nanopartículas del Metal/química
2.
Medicina (Kaunas) ; 57(11)2021 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-34833355

RESUMEN

Background and Objectives: Nutritional deficiencies are one of the main triggers for the development of gastrointestinal diseases, such as ulcerative colitis (UC). Therefore, the objective of the present work consisted of determining the nutrients present in the bone broth (BB) and evaluating their anti-inflammatory properties in a murine model of UC, induced by intrarectal administration of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS), and acetic acid (AcOH). The BB was prepared from the femur of bovine cattle and cooked in distilled water for 8 h at 100 ± 2 °C. Materials and Methods: The BB was administered ad libitum to BALB/c mice for 10 days before the induction of UC. Colon samples were collected for histological analysis and determination of cytokine expression levels by qPCR. Results: It was found that amino acids (AA) are the main nutritional contribution of BB, 54.56% of these correspond to essential AA. The prophylactic administration of BB in the murine model of UC reduced histological damage, decreased the expression of IL-1ß (61.12%), IL-6 (94.70%), and TNF-α (68.88%), and increased the expression of INF-γ (177.06%), IL-4 (541.36%), and IL-10 (531.97%). Conclusions: This study shows that BB has anti-inflammatory properties, and its consumption can decrease the symptoms of UC.


Asunto(s)
Colitis Ulcerosa/etiología , Colitis Ulcerosa/terapia , Desnutrición/complicaciones , Nutrientes/uso terapéutico , Animales , Antiinflamatorios/uso terapéutico , Bovinos , Citocinas , Modelos Animales de Enfermedad , Ratones , Ratones Endogámicos BALB C , Ácido Trinitrobencenosulfónico/uso terapéutico
3.
Transbound Emerg Dis ; 68(4): 2229-2238, 2021 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-33048439

RESUMEN

Beijing genotype Mycobacterium tuberculosis strains associate with increased virulence, resistance and/or higher transmission rates. This study describes a specific Beijing strain predominantly identified in the Panamanian province of Colon with one of the highest incidences of tuberculosis in the country. Retrospective mycobacterial interspersed repetitive unit/variable number of tandem repeats analysis of 42 isolates collected between January and August 2018 allowed to identify a cluster (Beijing A) with 17 (40.5%) Beijing isolates. Subsequent prospective strain-specific PCR-based surveillance from September 2019 to March 2020 confirmed the predominance of the Beijing A strain (44.1%) in this province. Whole-genome sequencing revealed higher-than-expected diversity within the cluster, suggesting long-term prevalence of this strain and low number of cases caused by recent transmission. The Beijing A strain belongs to the Asian African 3 (Bmyc13, L2.2.5) branch of the modern Beijing sublineage, with their closest isolates corresponding to cases from Vietnam, probably introduced in Panama between 2000 and 2012.


Asunto(s)
Mycobacterium tuberculosis , Tuberculosis/epidemiología , Animales , Beijing , Células Clonales , Genotipo , Repeticiones de Minisatélite , Mycobacterium tuberculosis/genética , Panamá/epidemiología , Prevalencia , Estudios Prospectivos , Estudios Retrospectivos
4.
Surg Radiol Anat ; 42(5): 603-606, 2020 May.
Artículo en Inglés | MEDLINE | ID: mdl-31707432

RESUMEN

PURPOSE: Understanding anatomical variations of the facial artery and its branches is important for dental and medical practitioners. METHODS: Routine cadaveric dissection of the head and neck was performed to demonstrate the origin and branches of the facial artery. RESULTS: Facial artery emerged from a common linguofacial trunk off the external carotid artery. On the face, the facial artery first gave off a pre-masseteric branch. Immediately after, an aberrant artery emerged from the facial artery that coursed along the ramus of the mandible, which upon further dissection and examination was found to anastomose with inferior alveolar artery within the ramus of the mandible. CONCLUSIONS: We report a unique anastomosis between facial and inferior alveolar arteries, vessels that have not been previously shown to communicate. This case report may provide useful information for oral and maxillofacial surgeons as well as dentists performing inferior alveolar nerve blocks.


Asunto(s)
Proceso Alveolar/irrigación sanguínea , Variación Anatómica/efectos de los fármacos , Arteria Carótida Externa/anomalías , Músculo Masetero/irrigación sanguínea , Anciano de 80 o más Años , Cadáver , Femenino , Humanos
5.
PLoS One ; 9(7): e102613, 2014.
Artículo en Inglés | MEDLINE | ID: mdl-25058417

RESUMEN

Mycoplasma pneumoniae causes a range of airway and extrapulmonary pathologies in humans. Clinically, M. pneumoniae is associated with acute exacerbations of human asthma and a worsening of experimentally induced asthma in mice. Recently, we demonstrated that Community Acquired Respiratory Distress Syndrome (CARDS) toxin, an ADP-ribosylating and vacuolating toxin synthesized by M. pneumoniae, is sufficient to induce an asthma-like disease in BALB/cJ mice. To test the potential of CARDS toxin to exacerbate preexisting asthma, we examined inflammatory responses to recombinant CARDS toxin in an ovalbumin (OVA) murine model of asthma. Differences in pulmonary inflammatory responses between treatment groups were analyzed by histology, cell differentials and changes in cytokine and chemokine concentrations. Additionally, assessments of airway hyperreactivity were evaluated through direct pulmonary function measurements. Analysis of histology revealed exaggerated cellular inflammation with a strong eosinophilic component in the CARDS toxin-treated group. Heightened T-helper type-2 inflammatory responses were evidenced by increased expression of IL-4, IL-13, CCL17 and CCL22 corresponding with increased airway hyperreactivity in the CARDS toxin-treated mice. These data demonstrate that CARDS toxin can be a causal factor in the worsening of experimental allergic asthma, highlighting the potential importance of CARDS toxin in the etiology and exacerbation of human asthma.


Asunto(s)
Asma/patología , Proteínas Bacterianas/toxicidad , Toxinas Bacterianas/toxicidad , Hiperreactividad Bronquial/patología , Sistema Respiratorio/efectos de los fármacos , Animales , Asma/inducido químicamente , Asma/inmunología , Hiperreactividad Bronquial/inducido químicamente , Hiperreactividad Bronquial/inmunología , Líquido del Lavado Bronquioalveolar/química , Líquido del Lavado Bronquioalveolar/citología , Quimiocina CCL17/biosíntesis , Quimiocina CCL17/inmunología , Quimiocina CCL22/biosíntesis , Quimiocina CCL22/inmunología , Eosinófilos/inmunología , Eosinófilos/patología , Humanos , Inflamación/inducido químicamente , Inflamación/inmunología , Inflamación/patología , Interleucina-13/biosíntesis , Interleucina-13/inmunología , Interleucina-4/biosíntesis , Interleucina-4/inmunología , Ratones , Ratones Endogámicos BALB C , Ovalbúmina/administración & dosificación , Proteínas Recombinantes/toxicidad , Sistema Respiratorio/inmunología , Sistema Respiratorio/patología , Células Th2/inmunología , Células Th2/patología
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