Effects of environmental enrichment on GLUT expression in the visual cortex of amblyopic rats.

OBJECTIVE: To investigate the potential changes of glucose metabolism and glucose transporter protein (GLUT) in the visual cortex of formally deprived amblyopic rats, as well as the effects of enriched environments on the levels of nerve conduction and glucose metabolism in the visual cortex of amblyopic rats. METHODS: 36 rats were randomly divided into three groups: CON + SE (n = 12), MD + SE (n = 12) and MD + EE (n = 12). The right eyelids of both MD + SE and MD + EE groups were sutured. After successful modelling, the MD + EE group was maintained in an enriched environment, and the other two groups were kept in the same environment. Pattern visual evoked potentials (PVEP) was used to confirm models' effect, glucose metabolism was analyzed by Micro-PET/CT (18F-FDG), and the protein as well as mRNA expression levels of GLUT were detected by Western Blot and quantitative RT-PCR (quantitative Reverse Transcription-Polymerase Chain Reaction) analyses, site of GLUT expression by immunofluorescence (IF). RESULTS: After suture modelling, both the MD + EE and MD + SE groups objective visual nerve conduction function decreased, the glucose metabolism in the visual cortex was markedly lower. After the enriched environment intervention, it recovered in the MD + EE group. The expression levels of GLUT1 and GLUT3 were increased in the MD + EE group in comparison with the MD + SE group. GLUT1 was primarily expressed on astrocytes and endothelial cells, but GLUT3 was mainly expressed on neurons. CONCLUSION: Enrichment of the environment exhibited a therapeutic effect on amblyopia, which could be related to the enhancement of glucose metabolism and GLUT expression in the visual cortex.

The role of vasoactive intestinal peptide (VIP) in atropine-related inhibition of the progression of myopia.

OBJECTIVE: This study aimed to investigate the potential involvement of vasoactive intestinal polypeptide (VIP) in myopia development and its contribution to the mechanism of action of the anti-myopia drug, atropine. METHODS: Thirty-three-week-old guinea pigs were randomly divided into normal control (NC, n = 10), monocularly form-deprived (FDM, n = 10), and FDM treated with 1% atropine (FDM + AT, n = 10) groups. The diopter and axial length were measured at 0, 2, and 4 weeks. Guinea pig eyeballs were removed at week four, fixed, and stained for morphological changes. Immunohistochemistry (IHC) and in situ hybridization (ISH) were performed to evaluate VIP protein and mRNA levels. RESULTS: The FDM group showed an apparent myopic shift compared to the control group. The results of the H&E staining were as follows: the cells of the inner/outer nuclear layers and retinal ganglion cells were disorganized; the choroidal thickness (ChT), blood vessel lumen, and area were decreased; the sclera was thinner, with disordered fibers and increased interfibrillar space. IHC and ISH revealed that VIP's mRNA and protein expressions were significantly up-regulated in the retina of the FDM group. Atropine treatment attenuated FDM-induced myopic shift and fundus changes, considerably reducing VIP's mRNA and protein expressions. CONCLUSIONS: The findings of elevated VIP mRNA and protein levels observed in the FDM group indicate the potential involvement of VIP in the pathogenesis and progression of myopia. The ability of atropine to reduce this phenomenon suggests that this may be one of the molecular mechanisms for atropine to control myopia.

Highly Pathogenic Avian Influenza A (H5N1) Virus in Swans, Central China, 2021.

Six highly pathogenic avian influenza (HPAI) H5N1 viruses (clade 2.3.4.4b) were detected in migratory birds in Hubei Province in November 2021. Phylogenetic analysis indicated that the viruses in the study included two different reassortants between H5N1 viruses that were circulating in Eurasia and low-pathogenic avian influenza viruses (LPAIVs). Several amino acid substitutions that contributed to the enhanced replication or virulence in mammals were observed in these viruses, suggesting a potential threat of the H5N1 viruses to human health. IMPORTANCE Here, we obtained the whole-genomes of six H5N1 viruses from dead or rescued wild birds in Hubei Province. These viruses were divided into two genotypes and had different evolutionary trajectories from previously reported H5N1 viruses in China. Extensive reassortment events between high-pathogenic (HP) and low-pathogenic (LP) avian influenza viruses (AIVs) were observed in these viruses. Moreover, a key amino acid analysis also suggests a potential threat of H5N1 viruses to public health. Our work explored the prevalent patterns of H5N1 viruses in wild birds and replenished the viral population data of H5N1 viruses in central China.

eHAPAC: A Privacy-Supported Access Control Model for IP-Enabled Wireless Sensor Networks.

The implementation of IP technology in wireless sensor networks has promoted the development of many smart scenarios. To enhance secure access in IP-enabled wireless sensor networks, access control to sensor nodes is a necessary process. However, access control currently faces two challenges, feasibility and preservation of user access privacy. In this paper, we propose eHAPAC, a novel privacy-preserving access control model for IP-enabled wireless sensor networks. The contributions of our paper include three parts. First, this paper integrates the Hidra access control protocol and APAC privacy-preserving model, addressing the issue of privacy-preserving access control in resource-constrained devices. Second, this paper proposes an enhanced Hidra protocol to implement the unlinkability of protocol message exchanges. Third, to solve the problem of third party credibility, this paper improves the group signature-based APAC model and utilizes blockchain technology to manage the storage and publication of public group signature keys. Security analysis and performance evaluation prove that our protocol is secure and effective.